响应面法优化超声波辅助北苍术多酚提取工艺及其DPPH自由基清除能力研究
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摘要
探讨北苍术多酚的最佳提取工艺,并评价北苍术多酚的DPPH自由基清除能力。采用超声波辅助提取方法,以乙醇浓度、液料比和超声时间进行单因素实验;以北苍术多酚提取量为响应值,进一步采用响应面法优化北苍术多酚的提取工艺条件,以抗坏血酸为对照,通过测定北苍术多酚清除DPPH自由基的能力来评价其抗氧化活性。实验结果表明,北苍术多酚最优提取工艺条件为:乙醇浓度74%(质量分数),液料比25mL/g,超声时间37 min,在此条件下北苍术多酚提取量达到(11.67±0.15)mg/g,与预测值相对误差为1.97%。该优化工艺可行;优化的北苍术多酚提取工艺科学、合理,且提取的北苍术多酚具有较好的DPPH自由基清除能力。
To optimize the extraction technology of polyphenols from Atractylodes chinensis(DC.) Koidz.and evaluate its antioxidant activity.The ultrasonic assisted extraction method was carried out.The effect of ethanol concentration,liquid-solid ratio and ultrasonic time on the yield of polyphenols from Atractylodes chinensis(DC.) Koidz.were investigated by single-factor experiment.Response surface methodology was then used to optimize the extraction technology conditions of polyphenols from Atractylodes chinensis(DC.) Koidz.The antioxidant activity was evaluated by DPPH free radicals scavenging method taking ascorbic acid as the control.The results showed that the optimal extraction technology of polyphenols was as follows:ethanol concentration 74%,liquid-solid ratio 25:1 mL/g and ultrasonic time 37 min,and the experimentally measured yield of polyphenols was(11.67±0.15) mg/g,with a relative error of only 1.97% compared to the model-predicted value.Thus,the conditions are optimal for polyphenols extraction.The IC_(50) of polyphenols was 2.10 mg/mL in DPPH radical scavenging,which was lower than that of scorbic acid.The optimization of ultrasonic assisted extraction of polyphenols from Atractylodes chinensis(DC.) Koidz.was reasonable and scientific,and the polyphenols from Atractylodes chinensis(DC.) Koidz.had antioxidant activity.
To optimize the extraction technology of polyphenols from Atractylodes chinensis(DC.) Koidz.and evaluate its antioxidant activity.The ultrasonic assisted extraction method was carried out.The effect of ethanol concentration,liquid-solid ratio and ultrasonic time on the yield of polyphenols from Atractylodes chinensis(DC.) Koidz.were investigated by single-factor experiment.Response surface methodology was then used to optimize the extraction technology conditions of polyphenols from Atractylodes chinensis(DC.) Koidz.The antioxidant activity was evaluated by DPPH free radicals scavenging method taking ascorbic acid as the control.The results showed that the optimal extraction technology of polyphenols was as follows:ethanol concentration 74%,liquid-solid ratio 25:1 mL/g and ultrasonic time 37 min,and the experimentally measured yield of polyphenols was(11.67±0.15) mg/g,with a relative error of only 1.97% compared to the model-predicted value.Thus,the conditions are optimal for polyphenols extraction.The IC_(50) of polyphenols was 2.10 mg/mL in DPPH radical scavenging,which was lower than that of scorbic acid.The optimization of ultrasonic assisted extraction of polyphenols from Atractylodes chinensis(DC.) Koidz.was reasonable and scientific,and the polyphenols from Atractylodes chinensis(DC.) Koidz.had antioxidant activity.
引文
[1]李云霞,李沈明,商春丽.承德发展苍术种植的可行性分析[J].中草药,2013,44(9):1215-1218.
[2]李万娟,郭艳玲,商春丽,等.北苍术化学成分的GC-MS分析[J].中国实验方剂学杂志,2016,22(6):66-70.
[3]PING J,JULIE T C,CORNELIUSEN B,et al.Lipase inhibition and antiobesity effect of Atractylodes lancea[J].Planta Med,2014,80(7):577-582.
[4]KOONRUNGSESOMBOON N,NA BANGCHANG K,KARBWANG J.Terapeutic potential and pharmacological activities of Atractylodes lancea(Tunb.)DC[J].Asian Pac J Trop Med,2014,7(6):421-428.
[5]RESCH M,STEIGEL A,CHEN Z L,et al.5-Lipoxygenase and cyclooxygenase-1 inhibitory active compounds from Atractylodes lancea[J].J Nat Prod,1998,61(3):347-350.
[6]XU K,YANG Y N,FENG Z M,et al.Six new compounds from Atractylodes lancea and their hepatoprotective activities[J].Bioorganic&Medicinal Chemistry Letters,2016,26(21):5187-5192.
[7]CHEN F,REN C G,ZHOU T,et al.A novel exopolysaccharide elicitor from endophytic fungus Gilmaniella sp.AL12 on volatile oils accumulation in Atractylodes lancea[J].Scientific Reports,2016,6:34735.
[8]ZHEN Ouyang,LEI Zhang,MING Zhao,et al.Identification and quantification of sesquiterpenes and polyacetylenes in Atractylodes lancea from various geographical origins using GC-MS analysis[J].Rev Bras Farmacogn,2012,22(5):957-963.
[9]KUO Xu,FENG Ziming,YANG Yanan,et al.Eight new eudesmane-and eremophilane-type sesquiterpenoids from Atractylodes lancea[J].Fitoterapia,2016,114:115-121.
[10]NAKAI Y,SAKAKIBARA I,HIRAKURA K,et al.A new acetylenic compound from the rhizomes of Atractylodes chinensis and its absolute configuration[J].Chem Pharm Bull,2005,53(12):1580-1581.
[11]WANG K T,CHEN LG,YANG L L,et al.Analysis of the sesquiterpenoids in processed Atractylodis Rhizoma[J].Chem Pharm Bull,2007,55(1):50-56.
[12]LIU Qiutao,ZHANG Shanshan,YANG Xihui,et al.Differentiation of essential oils in Atractylodes lancea and Atractylodes koreana by gas chromatography with mass spectrometry[J].J Sep Sci,2016,39(24):4773-4780.
[13]CHU Shasha,JIANG Guohua,LIU Zhilong.Insecticidal compounds from the essential oil of Chinese medicinal herb Atractylodes chinensis[J].Pest Manag Sci,2011,67(10):1253-1257.
[14]TAKAEDA O,MIKI E,TERABAYASHI S,et al.A comparative study on essential oil components of wild and cultivated Atractylodes lancea and A.chinensis[J].Planta Med,1996,62(5):444-449.
[15]赵光远,许艳华,陈美丽,等.石榴渣多酚提取及抗氧化活性研究[J].食品工业科技,2017,38(5):228-232.
[16]郭彩霞,任晓婷,张生万,等.响应面法优化超声波辅助提取猕猴桃果皮多酚工艺研究[J].食品工业科技,2017,38(11):244-250.
[17]张国强,郭晓东,薛文华,等.西藏野生卷叶黄精多酚的提取及其抗氧化活性分析[J].食品科学,2017,38(6):236-241.
[18]黄雅,陈华国,周欣,等.黔产接骨草中总多酚的含量测定及抗氧化活性研究[J].天然产物研究与开发,2017,29(2):255-263.
[19]卫阳飞,刘东花,张宏曦,等.窄叶鲜卑花叶中黄酮和多酚的超声提取工艺及抗氧化性研究[J].中药材,2017,40(1):158-163.
[20]徐彩红,马丽鑫,李桂杰,等.响应面法优化米糠多酚的醇提工艺[J].食品工业科技,2017,38(16):194-198.
[21]CHEW K,KHOO M,NG S,et al.Effect of ethanol concentration,extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts[J].International Food Research Journal,2011,18(4):571-578.
[22]YUE T,SHAO D,YUAN Y,et al.Ultrasound assisted extraction,HPLC analysis,and antioxidant activity of polyphenols from unripe apple[J].Journal of Separation Science,2012,35(16):2138-2145.
[23]胡明明,张国文,何力,等.响应面法优化超声提取花生壳多酚[J].南昌大学学报(理科版),2011,35(3):241-246.
[24]樊燕鸽,张娟梅,黄做华.响应面法优化怀菊水溶性总多酚的超声提取工艺[J].食品工业科技,2016,37(5):268-272.
[2]李万娟,郭艳玲,商春丽,等.北苍术化学成分的GC-MS分析[J].中国实验方剂学杂志,2016,22(6):66-70.
[3]PING J,JULIE T C,CORNELIUSEN B,et al.Lipase inhibition and antiobesity effect of Atractylodes lancea[J].Planta Med,2014,80(7):577-582.
[4]KOONRUNGSESOMBOON N,NA BANGCHANG K,KARBWANG J.Terapeutic potential and pharmacological activities of Atractylodes lancea(Tunb.)DC[J].Asian Pac J Trop Med,2014,7(6):421-428.
[5]RESCH M,STEIGEL A,CHEN Z L,et al.5-Lipoxygenase and cyclooxygenase-1 inhibitory active compounds from Atractylodes lancea[J].J Nat Prod,1998,61(3):347-350.
[6]XU K,YANG Y N,FENG Z M,et al.Six new compounds from Atractylodes lancea and their hepatoprotective activities[J].Bioorganic&Medicinal Chemistry Letters,2016,26(21):5187-5192.
[7]CHEN F,REN C G,ZHOU T,et al.A novel exopolysaccharide elicitor from endophytic fungus Gilmaniella sp.AL12 on volatile oils accumulation in Atractylodes lancea[J].Scientific Reports,2016,6:34735.
[8]ZHEN Ouyang,LEI Zhang,MING Zhao,et al.Identification and quantification of sesquiterpenes and polyacetylenes in Atractylodes lancea from various geographical origins using GC-MS analysis[J].Rev Bras Farmacogn,2012,22(5):957-963.
[9]KUO Xu,FENG Ziming,YANG Yanan,et al.Eight new eudesmane-and eremophilane-type sesquiterpenoids from Atractylodes lancea[J].Fitoterapia,2016,114:115-121.
[10]NAKAI Y,SAKAKIBARA I,HIRAKURA K,et al.A new acetylenic compound from the rhizomes of Atractylodes chinensis and its absolute configuration[J].Chem Pharm Bull,2005,53(12):1580-1581.
[11]WANG K T,CHEN LG,YANG L L,et al.Analysis of the sesquiterpenoids in processed Atractylodis Rhizoma[J].Chem Pharm Bull,2007,55(1):50-56.
[12]LIU Qiutao,ZHANG Shanshan,YANG Xihui,et al.Differentiation of essential oils in Atractylodes lancea and Atractylodes koreana by gas chromatography with mass spectrometry[J].J Sep Sci,2016,39(24):4773-4780.
[13]CHU Shasha,JIANG Guohua,LIU Zhilong.Insecticidal compounds from the essential oil of Chinese medicinal herb Atractylodes chinensis[J].Pest Manag Sci,2011,67(10):1253-1257.
[14]TAKAEDA O,MIKI E,TERABAYASHI S,et al.A comparative study on essential oil components of wild and cultivated Atractylodes lancea and A.chinensis[J].Planta Med,1996,62(5):444-449.
[15]赵光远,许艳华,陈美丽,等.石榴渣多酚提取及抗氧化活性研究[J].食品工业科技,2017,38(5):228-232.
[16]郭彩霞,任晓婷,张生万,等.响应面法优化超声波辅助提取猕猴桃果皮多酚工艺研究[J].食品工业科技,2017,38(11):244-250.
[17]张国强,郭晓东,薛文华,等.西藏野生卷叶黄精多酚的提取及其抗氧化活性分析[J].食品科学,2017,38(6):236-241.
[18]黄雅,陈华国,周欣,等.黔产接骨草中总多酚的含量测定及抗氧化活性研究[J].天然产物研究与开发,2017,29(2):255-263.
[19]卫阳飞,刘东花,张宏曦,等.窄叶鲜卑花叶中黄酮和多酚的超声提取工艺及抗氧化性研究[J].中药材,2017,40(1):158-163.
[20]徐彩红,马丽鑫,李桂杰,等.响应面法优化米糠多酚的醇提工艺[J].食品工业科技,2017,38(16):194-198.
[21]CHEW K,KHOO M,NG S,et al.Effect of ethanol concentration,extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts[J].International Food Research Journal,2011,18(4):571-578.
[22]YUE T,SHAO D,YUAN Y,et al.Ultrasound assisted extraction,HPLC analysis,and antioxidant activity of polyphenols from unripe apple[J].Journal of Separation Science,2012,35(16):2138-2145.
[23]胡明明,张国文,何力,等.响应面法优化超声提取花生壳多酚[J].南昌大学学报(理科版),2011,35(3):241-246.
[24]樊燕鸽,张娟梅,黄做华.响应面法优化怀菊水溶性总多酚的超声提取工艺[J].食品工业科技,2016,37(5):268-272.