分级醇沉柳茶多糖的含量与单糖组成及抗氧化性研究
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摘要
目的研究分级醇沉的柳茶多糖(SAPs)含量、单糖组成及抗氧化活性。方法采用水提、分级醇沉法制备SAPs,用硫酸-苯酚法测定多糖的含量,1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化HPLC法分析单糖的组成,红外分光光度法研究多糖的构型,DPPH·法评价分级多糖的抗氧化活性。结果分级醇沉SAPs含量的高低顺序为60%> 40%> 80%> 20%;葡萄糖、半乳糖和阿拉伯糖在各级醇沉SAPs三氟乙酸水解产物中的含量均最高,其次是半乳糖醛酸、葡萄糖醛酸、甘露糖和核糖,未检测到鼠李糖和木糖;分级醇沉SAPs的抗氧化性优劣顺序为40%> 80%> 60%> 20%,40%醇沉多糖浓度为0. 4 mg·m L~(-1)时对DPPH·清除能力与0. 04 mg·m L~(-1)抗坏血酸相当,其半数抑制浓度IC50=0. 235 mg·m L~(-1)。结论实验结果为柳茶多糖进一步化学、工艺和药理研究提供了参考。
OBJECTIVE To study the content,monosaccharide composition and antioxidant activity of different Sibiraea angustata polysaccharides( SAPs) which were precipitated using gradient concentrations of ethanol. METHODS Different SAPs were extracted by water and precipitated with gradient concentrations of ethanol. The content of polysaccharides was determined by sulfuric acid-phenol method. The composition of monosaccharide was determined by HPLC with PMP derivatization. Infrared spectrophotometry was used to study the configuration of SAPs. The antioxidant activity of SAPs was evaluated by DPPH· method. RESULTS The order of the contents of different SAPs was 60% > 40% > 80% > 20%. The contents of glucose,galactose and arabinose were the highest in the TFA hydrolysis products of different SAPs,followed by galacturonic acid,glucuronic acid,mannose and ribose,but no rhamnose and xylose were detected. The order of antioxidant activity was 40% > 80% > 60% > 20%. The scavenging ability of 0. 4 mg·m L~(-1) SAPs which were precipitated by 40% ethanol to DPPH· was almost the same as that of 0. 04 mg·m L~(-1) Vit C,and its IC50 value was 0. 235 mg·m L~(-1). CONCLUSION The research provides references for further studies on chemical constituents,techniques and pharmacology of SAPs.
OBJECTIVE To study the content,monosaccharide composition and antioxidant activity of different Sibiraea angustata polysaccharides( SAPs) which were precipitated using gradient concentrations of ethanol. METHODS Different SAPs were extracted by water and precipitated with gradient concentrations of ethanol. The content of polysaccharides was determined by sulfuric acid-phenol method. The composition of monosaccharide was determined by HPLC with PMP derivatization. Infrared spectrophotometry was used to study the configuration of SAPs. The antioxidant activity of SAPs was evaluated by DPPH· method. RESULTS The order of the contents of different SAPs was 60% > 40% > 80% > 20%. The contents of glucose,galactose and arabinose were the highest in the TFA hydrolysis products of different SAPs,followed by galacturonic acid,glucuronic acid,mannose and ribose,but no rhamnose and xylose were detected. The order of antioxidant activity was 40% > 80% > 60% > 20%. The scavenging ability of 0. 4 mg·m L~(-1) SAPs which were precipitated by 40% ethanol to DPPH· was almost the same as that of 0. 04 mg·m L~(-1) Vit C,and its IC50 value was 0. 235 mg·m L~(-1). CONCLUSION The research provides references for further studies on chemical constituents,techniques and pharmacology of SAPs.
引文
[1]赵汝能.甘肃中草药资源志[M].下册.兰州:甘肃科学技术出版社,2004:31-32.
[2]闫志慧,陈立书,朱仝飞,等.藏药柳茶叶乙醇水提取物中化学成分的鉴定[J].应用化学,2014,31(2):237-241.
[3]段博文,李运,刘昕,等.柳茶多糖对小鼠免疫功能的影响[J].中国中药杂志,2010,35(11):1466-1469.
[4]刘爱萍.植物多糖抗辐射效果的Meta分析及柳茶抗辐射损伤实验研究[D].兰州:兰州大学硕士学位论文,2014:1-59.
[5]石磊,张冬梅,齐洁,等.柳茶多糖的分离纯化及气相色谱-质谱分析[J].时珍国医国药,2009,20(9):2191-2192.
[6] Chen Z,Zhang W,Tang XY,et al. Extraction and characterization of polysaccharides from Semen cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC[J]. Carbohydr Polym,2016,144,263-270.
[7]卫阳飞,刘东花,张宏曦,等.窄叶鲜卑花叶中黄酮和多酚的超声提取工艺及抗氧化性研究[J].中药材,2017,40(1):158-163.
[8]施亚琴,杨培全,周俊国,等.红毛五加多糖的提取及含量测定[J].华西药学杂志,1994,9(2):73-74.
[2]闫志慧,陈立书,朱仝飞,等.藏药柳茶叶乙醇水提取物中化学成分的鉴定[J].应用化学,2014,31(2):237-241.
[3]段博文,李运,刘昕,等.柳茶多糖对小鼠免疫功能的影响[J].中国中药杂志,2010,35(11):1466-1469.
[4]刘爱萍.植物多糖抗辐射效果的Meta分析及柳茶抗辐射损伤实验研究[D].兰州:兰州大学硕士学位论文,2014:1-59.
[5]石磊,张冬梅,齐洁,等.柳茶多糖的分离纯化及气相色谱-质谱分析[J].时珍国医国药,2009,20(9):2191-2192.
[6] Chen Z,Zhang W,Tang XY,et al. Extraction and characterization of polysaccharides from Semen cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC[J]. Carbohydr Polym,2016,144,263-270.
[7]卫阳飞,刘东花,张宏曦,等.窄叶鲜卑花叶中黄酮和多酚的超声提取工艺及抗氧化性研究[J].中药材,2017,40(1):158-163.
[8]施亚琴,杨培全,周俊国,等.红毛五加多糖的提取及含量测定[J].华西药学杂志,1994,9(2):73-74.