干旱胁迫对窄叶鲜卑花种子萌发及幼苗生长的影响
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摘要
[目的]研究干旱胁迫对窄叶鲜卑花种子萌发及幼苗生长的影响,为野生种驯化和种群恢复提供参考。[方法]以窄叶鲜卑花种子为实验材料,采用不同浓度聚乙二醇(PEG-6000)模拟干旱胁迫环境,研究干旱协迫强度对窄叶鲜卑花种子的吸胀速率、发芽率、发芽势、发芽指数、活力指数和种子抗旱萌发指数及幼苗生长情况的影响。[结果]随着干旱胁迫程度的加剧,窄叶鲜卑花种子的吸水过程表现出急速吸水期、缓慢上升期和吸水平稳期3个阶段;其发芽率、发芽势、发芽指数、活力指数和种子抗旱萌发指数及苗高、根长,苗重与胁迫强度均呈负相关;当PEG浓度达30%时,种子萌发完全被抑制。另一方面,种子群体萌动、萌发和出苗达50%所需时间随胁迫强度的增加而越发延迟,且各阶段对环境临界水势要求不同,出苗阶段最为严格,表明种子出苗过程对环境水分胁迫较为敏感,耐旱能力较弱。[结论]干旱胁迫显著抑制了窄叶鲜卑花种子的萌发和幼苗生长,也说明水分是窄叶鲜卑花种子萌发和幼苗形成的主导因素之一,在较干旱地区种植需要考虑水分的供给。
[Objective]The effects of drought stress on the seed germination and seedling growth of Sibiraea angustata was investigated in order to provide reference for wild species domestication and population recovery.[Methods]To study the swellingrate,germination percentage,germination potential,germination index,vigor index,seed drought germination index and seedling growth in Sibiraea angustata under different concentrations of polyethylene glycol(PEG-6000)treatments.[Results]With the intensification of the stress level,the absorption process of the seeds of narrow leaves Sibiraea angustatashowed three stages:rapid water absorption period,slow rising period and water absorption period,and the germination rate,germination potential,germination index,vigor index and seed drought-resistance germination index,seedling height and root length,and the seedling weight were negatively correlated with the stress intensity.When the polyethylene glycol concentration reached 30%,the germination of seed was inhibited completely.On the other hand,seed germination and emergence,and 50%of the time delay were decreased with the increase of stress intensity.Among them,the most stringent stage is emergence stage,seed emergence process of environmental was more sensitive,and droughtcapability is weak.[Conclusion]Drought stress significantly inhibited the seed germination and seedling growth,it also suggested that water is one of the dominant factor for the formation of seed germination and seedling growth.Therefore,planting Sibiraea angustatain the arid areas requires consideration of water supply.
[Objective]The effects of drought stress on the seed germination and seedling growth of Sibiraea angustata was investigated in order to provide reference for wild species domestication and population recovery.[Methods]To study the swellingrate,germination percentage,germination potential,germination index,vigor index,seed drought germination index and seedling growth in Sibiraea angustata under different concentrations of polyethylene glycol(PEG-6000)treatments.[Results]With the intensification of the stress level,the absorption process of the seeds of narrow leaves Sibiraea angustatashowed three stages:rapid water absorption period,slow rising period and water absorption period,and the germination rate,germination potential,germination index,vigor index and seed drought-resistance germination index,seedling height and root length,and the seedling weight were negatively correlated with the stress intensity.When the polyethylene glycol concentration reached 30%,the germination of seed was inhibited completely.On the other hand,seed germination and emergence,and 50%of the time delay were decreased with the increase of stress intensity.Among them,the most stringent stage is emergence stage,seed emergence process of environmental was more sensitive,and droughtcapability is weak.[Conclusion]Drought stress significantly inhibited the seed germination and seedling growth,it also suggested that water is one of the dominant factor for the formation of seed germination and seedling growth.Therefore,planting Sibiraea angustatain the arid areas requires consideration of water supply.
引文
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[7]卫阳飞.柳茶对四氧嘧啶糖尿病小鼠降糖作用和对高脂血症大鼠游离脂肪酸影响的实验研究[D].甘肃兰州:兰州大学,2012.
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[10]李文娆,张岁岐,山仑.水分胁迫下紫花苜蓿和高粱种子萌发特性及幼苗耐旱性[J].生态学报,2009,29(6):556-563.
[11]何学青,呼天明,许岳飞,等.PEG-6000模拟水分胁迫对柳稷种子萌发及幼苗生长的影响[J].草地学报,2014,22(3):1007-0435.
[12] Scott S J,Jones R A,Williams W A.Review of dataanalysis methods for seed germination[J].Crop Science,1984,24(6):1192-1199.
[13]岳勇丽.干旱胁迫在不同品种杨梅苗期生长和生理生化特性[D].福建福州:福建农林大学,2012.
[14]杨景宁,王彦荣.PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J].草业学报,2012,21(6):23-29.
[15]徐建新,杨洁,吴景,等.聚乙二醇模拟干旱对新疆橡胶草种子萌发与幼苗生长的影响[J].干旱地区农业研究,2015,33(5):96-100.
[16]喻泽莉,何平,张春平,等.干旱胁迫对决明种子萌发及幼苗生理特性的影响[J].西南大学学报:自然科学版,2012,34(2):39-44.
[17]郝楠,李月明,吴玉群,等.玉米种子萌芽期抗旱性的初步筛选[J].辽宁农业科学,2010(5):40-42.
[18]孙霞.聚乙二醇(PEG)模拟干旱胁迫对干旱河谷5种木蓝种子萌发的影响[J].应用与环境生物学报,2010,16(3):317-322.
[19]杨旭东.PEG模拟干旱胁迫对向日葵种子种子活力影响的研究[D].内蒙古呼和浩特:内蒙古农业大学,2016.
[2] Hardegree S P,Emmerich W E.Effect of polyethylene glycol exclusion on the water potential of solution-saturated filter paper[J].Plant Physiology,1990,92(2):462-466.
[3]赵晓英,任继周,王彦荣,等.3种锦鸡儿种子萌发对温度和水分的响应[J].西北植物学报,2005,25(2):211-217.
[4]吴宁.川西北窄叶鲜卑花灌丛的类型和生物量及其与环境因子的关系[J].植物学报,1998,40(9):860-870.
[5]陶婷婷,濑井康雄,王天志,等.窄叶鲜卑花的化学成分[J].中国天然药物,2006,4(4):257-259.
[6]姚莉,鞠洋.窄叶鲜卑花促消化作用的实验研究[J].中国中西医结合消化杂志,2009,17(6):376-378.
[7]卫阳飞.柳茶对四氧嘧啶糖尿病小鼠降糖作用和对高脂血症大鼠游离脂肪酸影响的实验研究[D].甘肃兰州:兰州大学,2012.
[8]谢勇辉,俞颂华,余银芳,等.窄叶鲜卑花的化学成分及药理活性研究进展[J].江西中医学院学报,2014,26(5):97-100.
[9]王进,罗光宏,颜霞,等.干旱胁迫对苦豆子种子吸胀萌发和幼苗生长的影响[J].中草药,2011,42(9):1807-1811.
[10]李文娆,张岁岐,山仑.水分胁迫下紫花苜蓿和高粱种子萌发特性及幼苗耐旱性[J].生态学报,2009,29(6):556-563.
[11]何学青,呼天明,许岳飞,等.PEG-6000模拟水分胁迫对柳稷种子萌发及幼苗生长的影响[J].草地学报,2014,22(3):1007-0435.
[12] Scott S J,Jones R A,Williams W A.Review of dataanalysis methods for seed germination[J].Crop Science,1984,24(6):1192-1199.
[13]岳勇丽.干旱胁迫在不同品种杨梅苗期生长和生理生化特性[D].福建福州:福建农林大学,2012.
[14]杨景宁,王彦荣.PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J].草业学报,2012,21(6):23-29.
[15]徐建新,杨洁,吴景,等.聚乙二醇模拟干旱对新疆橡胶草种子萌发与幼苗生长的影响[J].干旱地区农业研究,2015,33(5):96-100.
[16]喻泽莉,何平,张春平,等.干旱胁迫对决明种子萌发及幼苗生理特性的影响[J].西南大学学报:自然科学版,2012,34(2):39-44.
[17]郝楠,李月明,吴玉群,等.玉米种子萌芽期抗旱性的初步筛选[J].辽宁农业科学,2010(5):40-42.
[18]孙霞.聚乙二醇(PEG)模拟干旱胁迫对干旱河谷5种木蓝种子萌发的影响[J].应用与环境生物学报,2010,16(3):317-322.
[19]杨旭东.PEG模拟干旱胁迫对向日葵种子种子活力影响的研究[D].内蒙古呼和浩特:内蒙古农业大学,2016.