3,4-亚甲二氧苯基甲酰吗啉与AMPA受体的结合作用研究
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摘要
目的研究3,4-亚甲二氧苯基甲酰吗啉(化合物1)与AMPA受体的结合作用。方法采用放射性配体受体结合试验测定化合物1与AMPA受体结合的IC50值;采用分子对接技术研究化合物1与AMPA受体的结合能力和结合模式。结果化合物1与AMPA受体结合的IC50值为1.74×10~(-9)mol·L~(-1),具有较强的结合能力;化合物1与AMPA受体的结合能为-5.46 kcal·mol~(-1),同样表明结合能力较强。结合的驱动力主要是氢键、疏水作用和静电作用。化合物1结构中O原子可与AMPA受体的Ser108、Ser242氨基酸残基形成氢键,这可能是该化合物与AMPA受体结合的重要位点。结论化合物1可与AMPA结合,且结合能力较强。
OBJECTIVE To investigate the binding of 3,4-methylenedioxyphenyl formylmorpholine(compound 1) to AMPA receptor. METHODS The potency of compound 1 binding to AMPA receptor was evaluated by radioligand binding assay. Meanwhile, the binding mode of compound 1 to AMPA receptor was evaluated by molecular docking. RESULTS Compound 1 showed high affinity of AMPA receptor with the IC50 value of 1.74×10~(-9) mol·L~(-1). Meanwhile, the results from molecular docking indicated that compound 1 could bind to AMPA receptor by high affinity with the ΔE value of -5.46 kcal·mol~(-1). Hydrogen bond, hydrophobic force and electrostatic interaction played key roles in binding. Moreover, the O atoms of compound 1 could form hydrogen bond with Ser108 and Ser242 of AMPA receptor, which was recommended as the key binding site. CONCLUSION The results demonstrate that compound 1 could bind to AMPA receptor with high affinity,.
OBJECTIVE To investigate the binding of 3,4-methylenedioxyphenyl formylmorpholine(compound 1) to AMPA receptor. METHODS The potency of compound 1 binding to AMPA receptor was evaluated by radioligand binding assay. Meanwhile, the binding mode of compound 1 to AMPA receptor was evaluated by molecular docking. RESULTS Compound 1 showed high affinity of AMPA receptor with the IC50 value of 1.74×10~(-9) mol·L~(-1). Meanwhile, the results from molecular docking indicated that compound 1 could bind to AMPA receptor by high affinity with the ΔE value of -5.46 kcal·mol~(-1). Hydrogen bond, hydrophobic force and electrostatic interaction played key roles in binding. Moreover, the O atoms of compound 1 could form hydrogen bond with Ser108 and Ser242 of AMPA receptor, which was recommended as the key binding site. CONCLUSION The results demonstrate that compound 1 could bind to AMPA receptor with high affinity,.
引文
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[2]WARD S E,BAX B D,HARRIESIES M.Challenges for and current status of research into positive modulators of AMPAreceptors[J].Br J Pharmacol,2010,160(2):181-190.
[3]LYNCH G.Glutamate-based therapeutic approaches:ampakines[J].Curr Opin Pharmacol,2006,6(1):82-88.
[4]WU X L,FAN W T,PAN Y L,et al.Synthesis,crystal structure and anti-fatigue effects of some benzamide derivatives[J].Molecules,2014,19(1):1034-1046.
[5]FAN W T,WU X L,PAN Y L,et al.1-(1,3-Benzodioxol-5-yl-carbo-nyl)piperidine,a modulator of a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor,ameliorates exercise-induced fatigue in mice[J].Bio Pharm Bull,2014,37(1):13-17.
[6]FAN W T,WU X L,PAN Y L,et al.Synthesis and antifatigue activities of new benzamide derivatives[J].Acta Pharm Sin(药学学报),2014,49(10):1442-1445.
[7]HONORE T,LAURIDCEN J,KROGSGAARD-LARSEN P.The binding of[3H]AMPA,a structural analogue of glutamic acid,to rat brain membranes[J].J Neurochem,1982,38(1):173-178.
[8]TIKHONOVA I G,LAVROV M I,PALYULIN V A,et al.The binding site for allosteric modulators of AMPA receptor[J].Dokl Biochem Biophys,2004(399):351-353.
[9]CHEN Y,MA X M,MEI Q B,et al.Anti-fatigue activity screening of novel positive allosteric modulators on AMPAreceptor[J].Chin Pharmacol Bull(中国药理学通报),2018,34(3):382-387.