升麻多糖的提取及其体外抗氧化活性研究
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摘要
目的:研究升麻多糖的提取及其体外抗氧化能力.方法:用水提醇沉法从升麻中提取多糖,并采用ABTS法测定升麻多糖的总抗氧化能力,应用比色法研究升麻多糖对DPPH·的清除能力.结果:当升麻多糖浓度为1.6 mg/mL时,其总抗氧化能力达到了0.64 mmol/L,对DPPH·的清除率也达到了67.94%.结论:升麻多糖具有较好的体外抗氧化能力,有望作为天然抗氧化剂得到开发.
Objective: To study the extraction and antioxidant capacity of cohosh polysaccharides in vitro.Method: Polysaccharides were extracted from cohosh by water extraction and alcohol precipitation.The total antioxidant capacity of cohosh polysaccharides was determined by ABTS method.The DPPH·scavenging capacity of cohosh polysaccharides was studied by colorimetry. Result: When the concentration of cohosh polysaccharides was 1.6 mg/mL,its total antioxidant capacity reached 0.64 mmol/L and the scavenging rate of DPPH·reached67.94%. Conclusion: Cohosh polysaccharides has good antioxidant capacity in vitro and is expected to be developed as a natural antioxidant.
Objective: To study the extraction and antioxidant capacity of cohosh polysaccharides in vitro.Method: Polysaccharides were extracted from cohosh by water extraction and alcohol precipitation.The total antioxidant capacity of cohosh polysaccharides was determined by ABTS method.The DPPH·scavenging capacity of cohosh polysaccharides was studied by colorimetry. Result: When the concentration of cohosh polysaccharides was 1.6 mg/mL,its total antioxidant capacity reached 0.64 mmol/L and the scavenging rate of DPPH·reached67.94%. Conclusion: Cohosh polysaccharides has good antioxidant capacity in vitro and is expected to be developed as a natural antioxidant.
引文
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[8]林晓月,齐珈娆,王丹丹,等.红景天多糖的提取及体外抗氧化活性研究[J].北华大学学报,2016,17(3):330-334.
[9]李培源,卢汝梅,苏炜,等.ABTS法测定福州薯蓣的抗氧化活性研究[J].湖北农业科学,2016,55(12):3173-3175.
[10]陈仕学,田艺,卢忠英,等.响应面法优化都匀毛尖茶多糖的提取及抗氧化性研究[J].食品工业,2015,36(9):83-88.
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[2]吴德松,卿晨.升麻药理学活性研究进展[J].医学综述,2009,15(6):918-920.
[3]姚梅芬,王岳峰,李展,等.升麻饮片质量研究标准[J].科技导报,2009,27(17):73-76.
[4]林玉萍,邱明华,李忠荣,等.升麻属植物的化学成分与生物活性研究[J].天然产物与开发,2002,14(6):58-76.
[5] Tian Ze,Pan Ruile,Si Jianyong.Cytotoxicity of Cycloar-tane Triterpenoids from Aerial Part of CimicifugaFoetida[J].Fitoterapia,2006,77(1):39-42.
[6] Xican Li,Jing Lin,Yaoxiang Gao,et al.Antioxidant ac-tivity and mechanism of Rhizoma Cimicifugae.Chemistry Central Journal,2012,6:140.
[7]吴彦.升麻多糖提取及其抗氧化作用[J].光谱实验室,2013,3(5):2444-2446.
[8]林晓月,齐珈娆,王丹丹,等.红景天多糖的提取及体外抗氧化活性研究[J].北华大学学报,2016,17(3):330-334.
[9]李培源,卢汝梅,苏炜,等.ABTS法测定福州薯蓣的抗氧化活性研究[J].湖北农业科学,2016,55(12):3173-3175.
[10]陈仕学,田艺,卢忠英,等.响应面法优化都匀毛尖茶多糖的提取及抗氧化性研究[J].食品工业,2015,36(9):83-88.
[11]马文华.浙贝母多糖体外抗氧化活性的研究[J].中华中医药学刊,2014,32(5):1191-1193.