苦参碱对IL-1β诱导的小鼠体外骨关节炎的作用研究
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摘要
目的探讨苦参碱对IL-1β诱导小鼠软骨细胞退行性变修复作用。方法提取3～5 dC 57乳鼠软骨细胞,分为空白对照组,骨关节炎(OA)组,骨关节炎加苦参碱(OA+Mat)组,分别处理24 h后,行HE染色,观察各组小鼠的细胞形态及数量,并提取各组小鼠的总RNA,RT-qPCR反应检测炎症及软骨标志基因的表达。结果与OA组相比,OA+Mat组细胞形态与空白对照组一样良好,细胞数量与空白对照组相近,且比OA组多,炎症及软骨标志基因的表达均比OA组高(P<0.05)。结论苦参碱能降低白介素1诱导的小鼠体外骨关节炎症标志基因的表达,对小鼠关节炎有治疗作用,且能上调关节炎细胞中软骨标志基因COL2a1的表达。
Objective Exploring the Effect of Matrine on IL-1β-induced degeneration of mouse chondrocyte. Methods Extraction of C57 mice chondrocytes from 3 to 5 days,divided into blank control group,osteoarthritis(OA) group,osteoarthritis plus Matrine(OA+Mat) group.After treatment for 24 h,HE staining was used to see the cell morphology and quantity of each group.Extracted the Total RNA of each group,detection of inflamMation and expression of cartilage marker genes by RT-qPCR reaction. Results Compared with the OA group,the cell morphology of the OA+Mat group was as good as the control group,the number of cells was similar to that of the control group,and it was more than the OA group. InflamMation and expression of cartilage marker genes were higher than OA(P<0.05). Conclusion Matrine can reduce the expression of inflamMatory marker genes in interleukin-1 induced vitro Mice osteoarthritis.It has a therapeutic effect on arthritis in mice and can up-regulate the expression of the cartilage marker gene COL2 a1 in arthritic cells.
Objective Exploring the Effect of Matrine on IL-1β-induced degeneration of mouse chondrocyte. Methods Extraction of C57 mice chondrocytes from 3 to 5 days,divided into blank control group,osteoarthritis(OA) group,osteoarthritis plus Matrine(OA+Mat) group.After treatment for 24 h,HE staining was used to see the cell morphology and quantity of each group.Extracted the Total RNA of each group,detection of inflamMation and expression of cartilage marker genes by RT-qPCR reaction. Results Compared with the OA group,the cell morphology of the OA+Mat group was as good as the control group,the number of cells was similar to that of the control group,and it was more than the OA group. InflamMation and expression of cartilage marker genes were higher than OA(P<0.05). Conclusion Matrine can reduce the expression of inflamMatory marker genes in interleukin-1 induced vitro Mice osteoarthritis.It has a therapeutic effect on arthritis in mice and can up-regulate the expression of the cartilage marker gene COL2 a1 in arthritic cells.
引文
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[15] 郑堰心,张丽,邓虹珠,等.槐定碱、苦参碱和苦豆碱对LPS诱发结肠上皮细胞炎症模型中细胞因子IL-6和TNF-α水平的影响[J].中国实验方剂学杂志,2014,20(8):133-136.
[2] Krishnan Y,Grodzinsky AJ.Cartilage diseases [M].Matrix Biology,2018:5.
[3] Mollon B,Kandel R,Chahal J.The clinical status of cartilage tissue regeneration in humans [J].Osteoarthritis and Cartilage,2013,21(12):1824-1833.
[4] Gargiulo S,Gamba P,Poli G,G.Metalloproteinases and metalloproteinase inhibitors in age-related diseases [J].Current pharmaceutical design,2014,20(18):2993-3018.
[5] Nelson FRT.The Value of Phenotypes in Knee Osteoarthritis Research [J].Open Orthop J,2018,12(1):105-114.
[6] Dong Y,Feng ZL,Chen HB,et al.Corni Fructus:a review of chemical constituents and pharmacological activities [J].Chin Med,2018,13(1):34.
[7] Jiao Q,Wang R,Jiang Y,et al.Study on the interaction between active components from traditional Chinese medicine andplasma proteins [J].Chem Cent J,2018,12(1):48.
[8] 陈丽,白军.顺铂联合8-硝基白杨素对人卵巢癌细胞裸鼠移植瘤生长的影响[J].中国现代应用药学,2013,30(3):246-250.
[9] Zhang XP,Liu DR,Shi Y.Study progress in therapeutic effects of traditional Chinese medicine monomer in severe acutepancreatitis [J].J Zhejiang Univ Sci B,2007,8(2):147-152.
[10] Wang J,Zhang JS.Study on antihyperlipidemia effects of Chinese medicine [J].Zhongguo Zhong Yao Za Zhi,2007,32(11):1005-1008.
[11] 张明发,沈雅琴.苦参碱抗炎和免疫抑制药理作用的研究进展[J].抗感染药学,2018,15(5):737-743.
[12] 任广慧,黄伟,肖旭,等.氧化苦参碱对胶原蛋白诱导大鼠关节炎症及T细胞亚群的影响[J].中国药学杂志,2012,47(5):350-353.
[13] Davatchi F,Sadeghi Abdollahi B,Mohyeddin M.Mesenchymal stem cell therapy for knee osteoarthritis:5 years follow-up of three patients [J].Int J Rheum Dis,2016,19(3):219-225.
[14] 胡屹屹,何孔旺,倪艳秀.苦参碱、水苏碱和防己诺林碱抗细菌内毒素的作用研究[J].江西农业大学学报,2012,34(1):129-133.
[15] 郑堰心,张丽,邓虹珠,等.槐定碱、苦参碱和苦豆碱对LPS诱发结肠上皮细胞炎症模型中细胞因子IL-6和TNF-α水平的影响[J].中国实验方剂学杂志,2014,20(8):133-136.