组合式压电喷头制备生物芯片的方法研究
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摘要
目的研发基于喷墨印刷技术的滚筒式生物芯片点样仪的组合压电喷头,介绍组合喷头的设计及其连续制备生物芯片的方法。方法该方法采用组合式压电喷头代替传统的单针头;用连续点样方式代替传统静态点样方式;用多样品同时喷点方式代替单个样品喷点方式,采用基于ARM9 S3C2416微处理器的触摸屏控制器来控制系统的运动,最后用激光跟踪仪测量了喷头连续制备生物芯片的精度。结果定位精度为8μm,重复精度为5μm,满足精度要求。结论提供了一种结构简单、方法可靠、精度高、性价比高的生物芯片制备方法。
A combination piezoelectric nozzle of a roller biochip microarrayer based on inkjet printing technology was researched and developed, and the design of combination nozzle and the method for continuous biochip preparation using this nozzle were emphatically described. This method used the combination piezoelectric nozzle instead of the traditional single needle, continuous spotting instead of the traditional static spotting, and simultaneous spraying of multiple samples instead of spraying of single sample. A touch screen controller based on ARM9 S3C2416 microprocessor was used to control the motion of the system, finally, the accuracy of continuous preparation of biochip using the nozzle was tested by a laser tracker. The test results showed that the positioning accuracy was 8 μm and the repeatability accuracy was 5 μm,which both met the accuracy requirement. This article provides a new method to prepare biochips with simple structure, reliable method, high accuracy and high cost-effectiveness.
A combination piezoelectric nozzle of a roller biochip microarrayer based on inkjet printing technology was researched and developed, and the design of combination nozzle and the method for continuous biochip preparation using this nozzle were emphatically described. This method used the combination piezoelectric nozzle instead of the traditional single needle, continuous spotting instead of the traditional static spotting, and simultaneous spraying of multiple samples instead of spraying of single sample. A touch screen controller based on ARM9 S3C2416 microprocessor was used to control the motion of the system, finally, the accuracy of continuous preparation of biochip using the nozzle was tested by a laser tracker. The test results showed that the positioning accuracy was 8 μm and the repeatability accuracy was 5 μm,which both met the accuracy requirement. This article provides a new method to prepare biochips with simple structure, reliable method, high accuracy and high cost-effectiveness.
引文
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[2]范金坪.生物芯片技术及其应用研究[J].中国医学物理学杂志,2009,26(2):1115—1118.FAN Jin-ping.Biochip Technology and Its Application[J].Chinese Journal of Medical Physics,2009,26(2):1115—1118.
[3]刘本明,石晶新,毕树生.生物芯片点样机器人系统设计[J].军民两用技术与产品,2007(11):42—44.LIU Ben-ming,SHI Jing-xin,BI Shu-sheng.Design of a Robot System for Making Biochip[J].Dual-use Technology and Products,2007(11):42—44.
[4]蔡锦达,齐建虹,顾豪.基于ARM9的滚筒式生物芯片点样仪的研发[J].仪器仪表学报,2013,34(10):2198—2204.CAI Jin-da,QI Jian-hong,GU Hao.Research and Development of Roller Biochip Microarrayer Based on ARM9[J].Chinese Journal of Scientific Instrument,2013,34(10):2198—2204.
[5]闫献勇,李建国,吴岚军.双闭环控制的生物芯片点样仪[J].中国仪器仪表,2004(11):37—38.YAN Xian-yong,LI Jian-guo,WU Lan-jun.Two Closedloop Controlled Biochip Microarrayer[J].Chinese Instruments,2004(11):37—38.
[6]ZHOUQ,YU F X,YU S Q.Study on the Control System of Microarrayer[C]//Intelligent Control and Automation,2006.WCICA 2006.The Sixth World Congress on IEEE,2006:8045—8049.
[7]齐建虹,蔡锦达.基于ARM9和自适应模糊PID算法的蜂窝纸板飞剪控制系统[J].包装工程,2013,34(21):75—79.QI Jian-hong,CAI Jin-da.Honeycomb Cardboard Flying Shear Control System Based on ARM9 and Adaptive Fuzzy PID Control Algorithm[J].Packaging Engineering,2013,34(21):75—79.
[8]周静,孙海滨,刘俊成.压电陶瓷在显示技术中的应用[J].硅酸盐通报,2009,28(5):2—7.ZHOU Jing,SUN Hai-bin,LIU Jun-cheng.Application of Piezoelectric Ceramic Materials on Display Technology[J].Bulletin of the Chinese Ceramic Society,2009,28(5):2—7.
[9]崔艳美,刘向锋,高志.大位移压电陶瓷驱动器的设计与试验[J].功能材料与器件学报,2007,13(6):507—511.CUI Yan-mei,LIU Xiang-feng,GAO Zhi.Design and Experiment Research on the Actuators for Large Displacement[J].Journal of Runctional Materials and Devices,2007,13(6):507—511.
[10]SAMUEL K M.Making Chips to Probe Genes[J].IEEE SPECTRUM,2001(38):54—60.
[11]王东爱,王岭松.包装机械的模块化设计研究[J].包装工程,2008,29(11):41—43.WANG Dong-ai,WANG Ling-song.Research on Modular Design of Packaging Machine[J].Packaging Engineering,2008,29(11):41—43.
[12]蔡锦达,顾豪,齐建虹.回转式高速点样仪的研制[J].机械设计,2014(11):77—82.CAI Jin-da,GU Hao,QI Jian-hong.Research and Development of Roller Biochip Microarrayer Based on ARM9[J].Chinese Journal of Scientific Instrument,2014(11):77—82.
[13]谢印忠,庄松林,张保洲.基于线阵CCD的光谱仪定标研究[J].仪器仪表学报,2011,32(3):546—550.XIE Yin-zhong,ZHUANG Song-lin,ZHANG Bao-zhou.Research on Calibration of Spectrometers Based on Linear CCD[J].Chinese Journal of Scientific Instrument,2011,32(3):546—550.
[14]李建国,霍荣玲,郭少鹏.生物芯片点样仪[J].现代科学仪器,2007(6):21—26.LI Jian-guo,HUO Rong-ling,GUO Shao-peng.Biochip Microarrayer[J].Modern Scientific Instruments,2007(6):21—26.
[15]孙莹,万秋华,佘容华,等.基于光电编码器的空间相机调焦控制系统设计[J].半导体光电,2009,30(6):970—974.SUN Ying,WAN Qiu-hua,SHE Rong-hua,et al.Design of Focusing Control System for Space Remote-Sensing Camera Based on Photoelectric Encoder[J].Semiconductor Optoelectronics,2009,30(6):970—974.
[16]黄浩,王晏民,范亮,等.基于激光跟踪测量系统的叶片平整度检测[J].北京建筑工程学院学报,2010,26(3):25—28.HUANG Hao,WANG Yan-ming,FAN Liang,et al.Detecting of the Blade Flatness Based on Laser Tracking Measurement System,2010,26(3):25—28.