不同营养物质和煤阶对厌氧发酵产氢特性的影响
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摘要
为进一步优选煤制生物氢的培养基条件和发酵底物,利用河南义马矿井水中分离出的产氢菌(CQ-2),在恒温箱培养条件下,讨论不同产氢培养基的碳源(葡萄糖、甘露醇、乳糖和柠檬酸钠)和氮源(豆面、蛋白胨、酵母膏、尿素、硝酸钾和硝酸铵)的选择,并进行不同煤阶煤的产氢实验。结果表明:培养基底物中最适合的氮源和碳源分别为蛋白胨和葡萄糖;低阶煤的产气效果较好,中、高阶煤产气效果较差;降解后煤的质量和元素成分都有不同程度的变化,与产气能力有较好的相关性;发酵后末端液相产物的pH值降低,Eh值上升,OD_(600)降低,证明CQ-2有良好的生物降解度和产氢能力。
In order to further optimize the medium condition and fermentation substrate of biological hydrogen by coal,in adoption of bacteria separation techniques,a strain of hydrogenogens named as CQ-2 was obtained from mine water of Yima colliery,He'nan Province,then an optimization of carbon and nitrogen sources was made and a simulation experiment for coal producing hydrogen was conducted.The results show that the optimal carbon source and nitrogen source are glucose and peptone respectively.CQ-2 has a better effect on decomposing low-rank coals to produce hydrogen than that it has on high-rank coals.The degraded coals have changed to varying degree,which performs a good relevance with the capacity of this hydrogenogens strain for decomposing coals and producing hydrogen as well.The pH value and the OD600 of the terminal liquid product have experienced a decline while the Eh value has growed,indicating the significant impact of the hydrogenogens strain on coal decomposition and hydrogen production.
In order to further optimize the medium condition and fermentation substrate of biological hydrogen by coal,in adoption of bacteria separation techniques,a strain of hydrogenogens named as CQ-2 was obtained from mine water of Yima colliery,He'nan Province,then an optimization of carbon and nitrogen sources was made and a simulation experiment for coal producing hydrogen was conducted.The results show that the optimal carbon source and nitrogen source are glucose and peptone respectively.CQ-2 has a better effect on decomposing low-rank coals to produce hydrogen than that it has on high-rank coals.The degraded coals have changed to varying degree,which performs a good relevance with the capacity of this hydrogenogens strain for decomposing coals and producing hydrogen as well.The pH value and the OD600 of the terminal liquid product have experienced a decline while the Eh value has growed,indicating the significant impact of the hydrogenogens strain on coal decomposition and hydrogen production.
引文
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[21]李建涛,刘向荣,皮淑颖,等.山西临汾褐煤微生物降解工艺条件的优化[J].煤炭转化,2017,40(2):65-72.LI Jianbo,LIU Xiangrong,PI Shuying,et al.Optimization of Technological Conditions for Microorganisms Degrading Shanxi Linfen Lignite[J].Coal Conversion,2017,40(2):65-72.
[22]苏现波,徐影,吴昱,等.盐度、pH对低煤阶煤层生物甲烷生成的影响[J].煤炭学报,2011,36(8):1302-1306.SU Xian Bo,XU Ying,WU Yu,et al.Effect of Salinity and pH on Biogenic Methane Production of Low Rank Coal[J].Journal of China Coal Society,2011,36(8):1302-1306.
[2]YANG Guang,WANG Jianlong.Co-fermentation of Sewage Sludge with Ryegrass for Enhancing Hydrogen Production:Performance Evaluation and Kinetic Analysis[J].Bioresource Technology,2017,243:1027-1036.
[3]ASSAWAMONGKHOLSIRI T,REUNGSANG A,PLANGKANG P,et al.Repeated Batch Fermentation for Photo-hydrogen and Lipid Production from Wastewater of a Sugar Manufacturing Plant[J].International Journal of Hydrogen Energy,2018,43(7):3605-3617.
[4]夏大平,陈曦,王闯,等.褐煤酸碱预处理-微生物气化联产H2-CH4的实验研究[J].煤炭学报,2017,42(12):3221-3228.XIA Daping,CHEN Xi,WANG Chuang,et al.Experimental Study on the Production of H2-CH4from Lignite Jointly with Acid-alkali Pretreatment-microbial Gasification[J].Journal of China Society,2017,42(12):3221-3228.
[5]NTAIKOU I,ANTONOPOULOU G,LYBERATOS G.Biohydrogen Production from Biomass and Wastes via Dark Fermentation:a Review[J].Waste and Biomass Valorization,2010,1(1):21-39.
[6]MOTA M P,PEIXOTO F M.Influence of Aerobic Fitness on Age-related Lymphocyte DNA Damage in Humans:Relationship with Mitochondria Respiratory Chain and Hydrogen Peroxide Production[J].AGE,2010,32(3):337-346.
[7]林明.高效产氢发酵新菌种的产氢机理及生态学研究[D].哈尔滨:哈尔滨工业大学,2002.LI Ming.Study on Mechanism and Ecology of Hydrogen Production by New Strains of High-efficiency Hydrogen-producing Fermentation[D].Harbin:Harbin Institute of Technology,2002.
[8]李永峰,任南琪,李建政,等.发酵产氢细菌分离培养的厌氧实验操作技术[J].哈尔滨工业大学学报,2004,36(12):1589-1592.LI Yongfeng,REN Nanqi,LI Jianzheng,et al.The Experimental Anaerobic Operation of Isolation and Culture on Hydrogenproducing and Fermentative Bacteria[J].Journal of Harbin Institute of Technology,2004,36(12):1589-1592.
[9]汤桂兰,孙振钧.厌氧污泥发酵制氢工艺试验研究[J].农业工程学报,2007,23(9):201-204.TANG Guilan,SUN Zhenjun.Technology for Biohydrogen Production Through Anaerobic Sewage Sludge Fermentation[J].Journal of Agricultural Engineering,2007,23(9):201-204.
[10]孙学习,李涛,计新静,等.玉米秸秆发酵制氢影响因素及变化规律研究[J].现代化工,2010,30(6):60-62.SUN Xuexi,LI Tao,JI Xinjing,et al.Parameter Effects and Mechanism of Fermentative Bio-hydrogen Production from Cornstalks[J].Modern Chemical Industry,2010,30(6):60-62.
[11]刘雪梅,任南琪,宋福南.微生物发酵生物制氢研究进展[J].太阳能学报,2008,29(5):544-549.LIU Xuemei,REN Nanqi,SONG Funan.Recent Advances in Biohydrogen Production by Microbe Fermentation[J].Journal of Solar Energy,2008,29(5):544-549.
[12]宋永民,陈洪章.汽爆秸秆高温固态发酵沼气的研究[J].环境工程学报,2008,2(11):1564-1570.SONG Yongmin,CHEN Hongzhang.Study on Biogas Production by Thermophilic Solid-state Fermentation from Steam Exploded Corn Stalk[J].Chinese Journal of Environmental Engineering,2008,2(11):1564-1570.
[13]HUANG Cunping,LINKOUS C A.Hydrogen Production via Photolytic Oxidation of Aqueous Sodium Sulfite Solutions[J].Environmental Science and Technology,2010,44(13):5283-5288.
[14]BLAFI-BAKA K,BUCSUA D,PIENTKAB Z,et al.Integration of Biohydrogen Fermentation and Gas Separation Processes to Recover and Enrich Hydrogen[J].International Journal of Hydrogen Energy,2006,31(11):1490-1495.
[15]苏现波,陈鑫,王惠风,等.不同厌氧发酵工艺对煤制氢的影响[J].煤炭转化,2013,36(2):16-19.SU Xianbo,CHEN Xin,WANG Huifeng,et al.Influence of Different Anaerobic Fermentation Technologies on Hydrogen Production from Coal[J].Coal Conversion,2013,36(2):16-19.
[16]YOKOI Haruhiko,MORI Shingo,HIROSE Jun,et al.H2Production from Starch by a Mixed Culture of Clostridium Butyricum and Rhodobacter sp.M-19[J].Biotechnology Letters,1998,20(9):895-899.
[17]于雷,高猛,张作明,等.高温厌氧菌CBZ-S-MEE2利用不同碳源发酵产氢的研究[J].中国酿造,2012,31(9):66-70.YU Lei,GAO Meng,ZHANG Zuoming,et al.Hydrogen Production from Various Carbon Sources Fermented by a Thermophilic Anaerobic Bacterium CBZ-S-MEE2[J].China Brewing,2012,31(9):66-70.
[18]付娜.拜氏梭菌RZF-1108产氢性能及其产氢代谢特性研究[D].哈尔滨:哈尔滨工业大学,2010.FU Na.Hydrogen Production Characters and Hydrogen Metabolism of Clostridium Beijerinckii RZF-1108[D].Harbin:Harbin Institute of Technology,2010.
[19]宋丽.厌氧产氢菌的分离筛选、诱变选育及其产氢特性的研究[D].成都:成都生物研究所,2008.SONG Li.Studies on the Isolation,Irradiation Screening and Characteristic of Anaerobic Hydrogen Producing Bacteria[D].Chengdu:Chengdu Institute of Biology,2008.
[20]RAYMUNDO-PINERO E,CAZORLA-AMOROS D,LINARES-SOLANO A,et al.Structural Characterization of N-containing Activated Carbon Fibers Prepared from a Low Softening Point Petroleum Pitch and a Melamine Resin[J].Carbon,2002,40(4):597-608.
[21]李建涛,刘向荣,皮淑颖,等.山西临汾褐煤微生物降解工艺条件的优化[J].煤炭转化,2017,40(2):65-72.LI Jianbo,LIU Xiangrong,PI Shuying,et al.Optimization of Technological Conditions for Microorganisms Degrading Shanxi Linfen Lignite[J].Coal Conversion,2017,40(2):65-72.
[22]苏现波,徐影,吴昱,等.盐度、pH对低煤阶煤层生物甲烷生成的影响[J].煤炭学报,2011,36(8):1302-1306.SU Xian Bo,XU Ying,WU Yu,et al.Effect of Salinity and pH on Biogenic Methane Production of Low Rank Coal[J].Journal of China Coal Society,2011,36(8):1302-1306.