豨莶草治疗MRL/lpr小鼠狼疮性肾炎疗效初观察
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摘要
目的观察豨莶草对MRL/lpr小鼠狼疮性肾炎的改善情况。方法通过观察小鼠的一般状况以及检测尿蛋白含量、血清中IL-1β、IL-6、TNF-α水平和肾组织病理的改变来评价豨莶草对狼疮性肾炎疗效。结果与对照组相比,豨莶草可以改善小鼠的一般状况,提高小鼠的体质量,显著降低尿蛋白含量、减少血清中炎性细胞因子IL-6、TNF-α的水平,肾脏脏组织病理损害明显减轻;各组生存分析结果显示无统计学差异(P> 0.05)。结论豨莶草可在一定程度上起到缓解狼疮性肾炎的作用,且豨莶草副作用相较于强的松更低,可为狼疮性肾炎的治疗提供新的可能。
Objective To observe the therapeutic effect of Siegesbeckiae Herba on lupus nephritis(LN) in MRL/lpr mice. Methods Mice are weighed and the urinary protein content is detected by using bicinchoninic acid(BCA), the serum level of inflammatory cytokines IL-1β, IL-6, TNF-α are measured and the pathological changes of the kidneys are examined microscopically. Results Compared with the control group, Siegesbeckiae Herba can improve the appearance and the body weight of MPL/lpr mice. The urinary protein content in the Siegesbeckiae Herba group is significantly lower than that in the control group, it can also decreased the level of inflammatory cytokines IL-6 and TNF-α. And the pathological damage of mice kidneys are obviously alleviated. The survival curve show no statistical difference. Conclusion Siegesbeckiae Herba plays a key role in therapeutic effect on lupus nephritis. Also, the side effects of the Siegesbeckiae Herba is lower than that of prednisone group, which could be a new possible option for the treatment of lupus nephritis.
Objective To observe the therapeutic effect of Siegesbeckiae Herba on lupus nephritis(LN) in MRL/lpr mice. Methods Mice are weighed and the urinary protein content is detected by using bicinchoninic acid(BCA), the serum level of inflammatory cytokines IL-1β, IL-6, TNF-α are measured and the pathological changes of the kidneys are examined microscopically. Results Compared with the control group, Siegesbeckiae Herba can improve the appearance and the body weight of MPL/lpr mice. The urinary protein content in the Siegesbeckiae Herba group is significantly lower than that in the control group, it can also decreased the level of inflammatory cytokines IL-6 and TNF-α. And the pathological damage of mice kidneys are obviously alleviated. The survival curve show no statistical difference. Conclusion Siegesbeckiae Herba plays a key role in therapeutic effect on lupus nephritis. Also, the side effects of the Siegesbeckiae Herba is lower than that of prednisone group, which could be a new possible option for the treatment of lupus nephritis.
引文
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[12]丁朝霞,杨少锋,吴启富,等.白芍总苷对MRL/lpr小鼠狼疮性肾炎的影响[J].南方医科大学学报,2011,31(4):656-660.
[13]Swenson J,Olgun S,Radjavi A,et al.Clinical efficacy of buprenorphine to minimize distress in MRL/lpr mice[J].European Journal of Pharmacology,2012,567(1):67-76.
[14]王婷,梅静.中药在减轻肾病综合征激素副作用过程中的应用[J].新疆中医药,2011,29(2):24-25.
[15]夏正坤.糖皮质激素临床应用再认识[J].医学研究生学报,2018(2):113-117.
[16]Zhang Z,Liu D,Zhang X,et al.Erythropoietin Treatment Ameliorates Lupus Nephritis of MRL/lpr Mice[J].Inflammation,2018:1-12.
[17]Nie X,Deng R,Xiang L,et al.Reno-protective effect and mechanism study of Huang Lian Jie Du Decoction on lupus nephritis MRL/lpr mice[J].BMCComplementary and Alternative Medicine,2016,16(1):448.
[18]Alberto S M,Pablo M L,Mario S J,et al.Effects of 12-week Aerobic Exercise on Arterial Stiffness,Inflammation and Cardiorespiratory Fitness in Women with Systemic LUPUS Erythematosus:Non-Randomized Controlled Trial[J].J Clin Med,2018,11(24):6444-6452.
[19]滕天立,徐世芳,陈峰阳,等.中药豨莶草的化学成分及其药理作用研究进展[J].中国现代应用药学,2015,32(2):250-260.
[20]傅旭春,蒋芳萍,范珈祯,等.豨莶草对单克隆抗体组合诱导的小鼠类风湿关节炎作用[J].浙江大学学报(医学版),2013,42(5):556-560.
[2]Zharkova O,Celhar T,Cravens P D,et al.Pathways leading to an immunological disease:systemic lupus erythematosus[J].Rheumatology,2017,56(Suppl 1):155-166.
[3]Pan Q,Li Y,Ye L,et al.Geographical distribution,a risk factor for the incidence of lupus nephritis in China[J].BMC Nephrology,2014,15(1):67-67.
[4]国家药典委员会.中华人民共和国药典[S].北京:中国医药科技出版社,2010.
[5]谢欢,王鹏.豨莶药性考[J].江西中医药大学学报,2016,28(5):7-8.
[6]于静,王建欣,苏素文,等.豨莶草提取物对抗多柔比星致大鼠急性肝肾损伤的作用及其机制[J].医药导报,2014,33(4):422-426.
[7]Wang R,Chen W H,Shi Y P.Ent-kaurane and ent-pimarane diterpenoids from Siegesbeckia pubescens.[J].Journal of Natural Products,2010,73(1):17-21.
[8]Wu J,Qu Y,Deng J,et al.Molecular docking studies of kirenol a traditional Chinese medicinal compound against rheumatoid arthritis cytokine drug targets(TNF-α,IL-1 and IL-6)[J].Biomedical Research,2017,28(5):1992-1995.
[9]张媛.豨莶草提取物对系统性红斑狼疮治疗作用初步研究[D].沈阳:辽宁中医药大学,2011.
[10]毋静,曲源,赖蓉,等.豨莶草提取物治疗系统性红斑狼疮及减少糖皮质激素副作用临床研究[C].全国中西医结合风湿病学术会议,2014:91-93.
[11]汪卫,于健宁,刘小贤.槐杞黄颗粒对MRL/Lpr狼疮小鼠肾损伤治疗作用的影响[J].中国中西医结合杂志,2018,38(4):478-483.
[12]丁朝霞,杨少锋,吴启富,等.白芍总苷对MRL/lpr小鼠狼疮性肾炎的影响[J].南方医科大学学报,2011,31(4):656-660.
[13]Swenson J,Olgun S,Radjavi A,et al.Clinical efficacy of buprenorphine to minimize distress in MRL/lpr mice[J].European Journal of Pharmacology,2012,567(1):67-76.
[14]王婷,梅静.中药在减轻肾病综合征激素副作用过程中的应用[J].新疆中医药,2011,29(2):24-25.
[15]夏正坤.糖皮质激素临床应用再认识[J].医学研究生学报,2018(2):113-117.
[16]Zhang Z,Liu D,Zhang X,et al.Erythropoietin Treatment Ameliorates Lupus Nephritis of MRL/lpr Mice[J].Inflammation,2018:1-12.
[17]Nie X,Deng R,Xiang L,et al.Reno-protective effect and mechanism study of Huang Lian Jie Du Decoction on lupus nephritis MRL/lpr mice[J].BMCComplementary and Alternative Medicine,2016,16(1):448.
[18]Alberto S M,Pablo M L,Mario S J,et al.Effects of 12-week Aerobic Exercise on Arterial Stiffness,Inflammation and Cardiorespiratory Fitness in Women with Systemic LUPUS Erythematosus:Non-Randomized Controlled Trial[J].J Clin Med,2018,11(24):6444-6452.
[19]滕天立,徐世芳,陈峰阳,等.中药豨莶草的化学成分及其药理作用研究进展[J].中国现代应用药学,2015,32(2):250-260.
[20]傅旭春,蒋芳萍,范珈祯,等.豨莶草对单克隆抗体组合诱导的小鼠类风湿关节炎作用[J].浙江大学学报(医学版),2013,42(5):556-560.