厚朴多酚的提取及体内抗氧化研究
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摘要
抗氧化活性是研究动物抗衰老的重要方面。以厚朴树皮为原料,比较超声波法和浸提法提取厚朴多酚的含量差异,并研究了厚朴多酚对KM小鼠体内抗氧化活性和有害性。结果表明:采用超声波提取法提取得到的多酚纯度为76.67%,高于浸提法(64.11%);厚朴多酚进行分组对比试验,灌胃饲养试验表明:KM小鼠血清中抑制羟自由基的能力和抗氧化成分(T-SOD、CAT、GSH-Px)的活性都得到提升,而有害的成分丙二醛MDA的含量有所降低。因此,厚朴多酚有抗氧化作用,有可能使动物健康生活,从而达到抗衰老的效果。
Antioxidant activity is an important aspect of studying animal anti-aging. Using the bark of Magnolia officinalis as raw material, the extraction content of magnolia was compared by ultrasonic method and extraction method. Choosing polyphenols of Magnolia officinalis to test the antioxidant activity and harmfulness of KM mice will contribute to further research on animal health and longevity. The purity of polyphenols extracted by ultrasonic extraction was 76.67%, which was higher than extraction method(64.11%); the results of group comparison intragastric feeding test of polyphenols of Magnolia officinalis showed that the activity of hydroxyl radical suppression and the activity of antioxidant components(T-SOD, CAT and GSH-Px) in KM mouse serum, while the content of harmful components malondialdehyde MDA decreased. Therefore, the polyphenols of Magnolia officinalis have antioxidative effect, which may make the animals live healthfully, so as to achieve the anti-aging effect.
Antioxidant activity is an important aspect of studying animal anti-aging. Using the bark of Magnolia officinalis as raw material, the extraction content of magnolia was compared by ultrasonic method and extraction method. Choosing polyphenols of Magnolia officinalis to test the antioxidant activity and harmfulness of KM mice will contribute to further research on animal health and longevity. The purity of polyphenols extracted by ultrasonic extraction was 76.67%, which was higher than extraction method(64.11%); the results of group comparison intragastric feeding test of polyphenols of Magnolia officinalis showed that the activity of hydroxyl radical suppression and the activity of antioxidant components(T-SOD, CAT and GSH-Px) in KM mouse serum, while the content of harmful components malondialdehyde MDA decreased. Therefore, the polyphenols of Magnolia officinalis have antioxidative effect, which may make the animals live healthfully, so as to achieve the anti-aging effect.
引文
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[2]苏德辉.厚朴皮中厚朴酚与和厚朴酚分离与纯化方法的研究[D].南宁:广西大学,2012.
[3]乔青青.厚朴抑菌成分的高效提取及应用研究[D].郑州:河南工业大学,2012.
[4]邓世明,程永现,周俊,等.长缘厚朴中的新苯醌及新木脂素类化合物[J].植物分类与资源学报,2001,23(1):121-125.
[5]姚明光,陈立新,代岩,等.厚朴皮与厚朴叶酚性成分的比较[J].中草药,1991,22(8):351-351.
[6]张光弼,张丽兰,高占武,等.不同产地厚朴的含量测定[J].黑龙江医药,1998,11(5):278-279.
[7]王洪燕,周先礼,黄帅,等.凹叶厚朴中生物碱成分的研究[J].华西药学杂志,2007,22(l):30-33.
[8]龙飞.厚朴资源综合利用研究——厚朴叶药用价值的初步研究[D].成都:成都中医药大学,2003.
[9]Jackson J H,Gajewski E,Schraufstatter I U,et al.Damage to the bases in DNA induced by stimulated human neutrophils[J].Journal of Clinical Investigation,1989,84(5):1644.
[10]Ames B N,Shigenaga M K,Hagen T M.Oxidants,antioxidants,and the degenerative diseases of aging[J].Proceedings of the National Academy of Sciences,1993,90(17):7915-7922.
[11]袁琳,孙东坡,程寒,等.厚朴酚两种提纯工艺的比较研究[J].天然产物研究与开发,2013,25(10):1445-1447.
[12]Chang C,Hsu Y,Lin M.Magnolol protects against cerebral ischaemic injury of rat heatstroke[J].Clinical&Experimental Pharmacology&Physiology,2003,30(5-6):387-392.
[13]Li H B.Protective effect of magnolol on TBHP-induced injury in H460cells partially via a p53 dependent mechanism[J].Archives of Pharmacal Research,2007,30(7):850-857.
[14]付云贺.厚朴酚抗炎作用及机制研究[D].吉林长春:吉林大学.
[15]李胜华,伍贤进,郁建平,等.多穗柯总黄酮提取工艺及其含量动态变化研究[J].食品科学,2008,29(6):139-141.
[16]徐辉艳,孙晓东,张佩君,等.红枣汁中总酚含量的福林法测定[J].食品研究与开发,2009,30(3):126-128.
[17]陈哲.银杏多肽的提取、分离纯化及体内抗氧化活性研究[D].合肥:合肥工业大学,2012.
[18]保志娟,杨雪琼,邹永明,等.厚朴酚与和厚朴酚清除DPPH·的作用[J].云南大学学报(自然科学版),2005,27(1):60-63.
[19]孟洁,胡迎芬,胡博路,等.厚朴抗氧化作用研究[J].中国油脂,2000,25(4):30-32.
[20]胡迎芬,孟洁.厚朴提取物对猪油抗氧化作用的研究[J].食品科学,2000,21(7):29-31.