厚朴叶不同溶剂提取物抗氧化活性比较分析
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摘要
采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除法,2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2/-azino-bis(3-ethylbenzthiazoline-6-sulonic acid),ABTS]自由基清除法以及铁离子还原能力(ferric reducing antioxidant power,FRAP)测定,评价厚朴叶7种不同溶剂提取物的抗氧化活性,并考察总酚含量与抗氧化活性的关系。结果表明,厚朴叶不同溶剂提取物具有良好的抗氧化活性,但抗氧化能力存在一定差异。其中正丁醇提取物的DPPH自由基清除率和ABTS自由基清除率最高,EC50值分别为40.67μg/m L和26.67μg/m L;乙醇提取物的铁离子还原能力最强,在浓度为0.1 mg/m L时,其FRAP值为24.54%;含量测定显示,乙醇提物中总酚含量最高,为174.14 mg/g;相关系数表明,总酚含量与抗氧化能力具有较好的相关性,提示乙醇可以作为厚朴叶抗氧化活性物质提取的优选溶剂。
Seven different solvent extracts of Magnolia officinalis leaves were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay,2,2′-azino-bis(3-ethylbenzthiazoline-6-sulonic acid)(ABTS)free radical scavenging assay and ferric reducing antioxidant power(FRAP)assay. The relationship between the antioxidant activity and the content of total phenol was investigated. The results showed that all the extracts of Magnolia officinalis leaves had good antioxidant activity. The n-butyl alcohol extract had the highest DPPH radical scavenging and ABTS radical scavenging activity,which EC50 were 40.67 μg/m L and 26.67 μg/m L,respectively. The ethanol extract had the strongest ferric reducing antioxidant power,with the FRAP value of24.54 % at a concentration of 0.1 mg/m L. The content of total phenol in ethanol extract was highest 174.14 mg/g.Correlation analysis showed there was a good correlation between total phenol content and antioxidant capacity.It was suggested that ethanol could be one of the best solvents used for extract antioxidant ingredients from Magnolia officinalis leaves.
Seven different solvent extracts of Magnolia officinalis leaves were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay,2,2′-azino-bis(3-ethylbenzthiazoline-6-sulonic acid)(ABTS)free radical scavenging assay and ferric reducing antioxidant power(FRAP)assay. The relationship between the antioxidant activity and the content of total phenol was investigated. The results showed that all the extracts of Magnolia officinalis leaves had good antioxidant activity. The n-butyl alcohol extract had the highest DPPH radical scavenging and ABTS radical scavenging activity,which EC50 were 40.67 μg/m L and 26.67 μg/m L,respectively. The ethanol extract had the strongest ferric reducing antioxidant power,with the FRAP value of24.54 % at a concentration of 0.1 mg/m L. The content of total phenol in ethanol extract was highest 174.14 mg/g.Correlation analysis showed there was a good correlation between total phenol content and antioxidant capacity.It was suggested that ethanol could be one of the best solvents used for extract antioxidant ingredients from Magnolia officinalis leaves.
引文
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[2]权婧婧,房艺丹,雷迎,等.厚朴叶的开发利用研究进展[J].安徽农业科学,2011,39(33):20396-20397
[3]WANG T,CHEN F,CHEN Z,et al.Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO[J].World Journal of Gastroenterology,2004,10(15):2205-2208
[4]黄晓燕,卫莹芳,张盈娇,等.高效液相色谱法测定厚朴叶不同采收期中厚朴酚、和厚朴酚的含量[J].中国中药杂志,2005,30(9):717-718
[5]HAJIMA HMOODI M,FARAMARZI MA,MOHAMMADI N,et al.Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae[J].Journal of Applied Phycology,2010,22(1):43-50
[6]谢静,费正彬,陈玉莲,等.厚朴叶水提物抗腹泻效果研究[J].安徽农业科学,2017,45(19):107-109
[7]刘晓鹏,姜宁,向东山,等.厚朴叶中厚朴酚及和厚朴酚的提取与测定[J].中国医院药学杂志,2007,27(5):694-696
[8]吴锦玉,吴岩斌,吴建国,等.厚朴叶总黄酮的超声提取工艺研究[J].福建中医药,2013,44(4):51-52
[9]邓芬,张芳芹,王烨,等.厚朴叶子中多糖的超声法提取及抗氧化活性研究[J].食品科技,2011(8):173-175
[10]赖普辉,刘存芳,李星彩,等.不同季节厚朴树叶挥发性物质分析及其抗氧化活性的研究[J].食品工业科技,2012,33(19):101-104
[11]NORIE T,KAZUTAKA N,KANJI I.Antioxidative Capacity of Extracts and Constituents in Cornus capitata Adventitious Roots[J].Journal of Agricultural&Food Chemistry,2003,51(20):5906-5910
[12]GARZON G A,WROLSTAD R E.Major anthocyanins and antioxidant activity of Nasturtium flowers(Tropaeolum majus)[J].Food Chemistry,2009,114(1):44-49
[13]贾东升,李荣乔,谢晓亮,等.连翘叶不同溶剂提取物体外抗氧化活性研究[J].食品研究与开发,2016,37(2):14-18
[14]张燕,张洪斌,贺立静,等.一点红不同营养器官中总黄酮和总酚含量测定[J].中国医药导报,2009,6(36):73-75
[15]ZHANG Hao,JIANG Lu,YE Shu,et al.Systematic evaluation of antioxidant capacities of the ethanolic extract of different tissues of jujube from China[J].Food and Chemical Toxicology,2010,48(6):1461-1465
[16]REN L,HEMAR Y,PERERA C O,et al.Antibacterial and antioxidant activities of aqueous extracts of eight edible mushrooms[J].Bioactive Carbohydrates&Dietary Fibre,2014,3(2):41-51
[17]刘芸,仇农学,杨玺玉.葡萄皮渣提取物总酚含量及体外抗氧化活性、抑菌活性[J].食品科学,2011,32(1):5-9
[18]OZTURK M.Anticholinesterase and antioxidant activities of Savoury(Satureja thymbra L.)with identified major terpenes of the essential oil[J].Food Chemistry,2012,134(1):48-54
[19]FIRIZY M,TALEBPOUR Z,SONBOLI A.Essential oil composition and antioxidant activities of the various extracts of Tanacetum sonbolii Mozaff.(Asteraceae)from Iran[J].Natural Product Research,2012,26(23):2204-2207
[20]郭淼,宋江峰,豆海港.超声波辅助提取草果精油及其抗氧化活性研究[J].食品研究与开发,2017,38(16):58-61
[21]杨少辉,宋英今,王洁华,等.雪莲果体外抗氧化和自由基清除能力[J].食品科学,2010,31(17):166-169
[22]罗兴武,吴菲.豆腐柴保健饮料的研制[J].食品工业科技,2012(2):246-249
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[24]涂宗财,傅志丰,王辉,等.红薯叶不同溶剂提取物抗氧化性及活性成分鉴定[J].食品科学,2015,36(17):1-6
[25]李晨,肖林刚,胡亚鹏,等.金针菇提取物不同组分的抗氧化活性[J].食品研究与开发,2016,37(23):5-9
[26]刘雨潇,周骁昳,刘峰,等.玫瑰茄提取物多酚含量与抗氧化作用研究[J].食品研究与开发,2011,32(3):75-78
[27]谈利红.体外抗氧化评价体系在中药中的拓展应用[D].重庆:重庆医科大学,2016
[28]范琪.厚朴抗氧化活性成分的提取分离及其抗氧化活性测试研究[D].重庆:重庆大学,2014
[29]蔡文国,吴卫,代沙,等.不同种质鱼腥草总酚、黄酮含量及其抗氧化活性[J].食品科学,2013,34(7):42-46
[30]刘曦,祝连彩,王伯初.蓝莓叶不同溶剂提取物抗氧化活性研究[J].食品工业科技,2013,34(12):101-105
[31]崔施展,谢晓亮,贾东升,等.黑枸杞不同溶剂提取物抗氧化活性[J].食品研究与开发,2017,38(9):38-41
[32]宋莉,倪世峰,李亚君,等.夏枯草不同溶剂提取物抗氧化活性研究[J].食品研究与开发,2016,37(17):32-35
[33]陈智坤,梁呈元,李维林,等.薄荷不同溶剂提取物抗氧化活性的研究[J].食品工业科技,2013,34(3):100-103
[34]TUPE P,SAKAT S,NAGOTI D,et al.Comparative study of Mentha arvensis Linn whole plant extracts for antioxidant and antidepressant activity[J].Plana Medica,2010,28(4):527-537