慢性牙周炎患者外周血CD4~+CD25~+调节性T细胞表达
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摘要
目的探讨外周血单个核细胞CD4~+CD25~+调节性T细胞(Tregulatory, Treg)在慢性牙周炎患者中的表达。方法慢性牙周炎患者40例,其中轻中度组22例和重度组18例;同期31例牙周健康者为对照组。采用流式细胞术检测3组CD4~+CD25~+CD127~(low/-)Treg、CD4~+CD25~+FoxP3~+Treg表达;采用ELISA法测定3组血浆转化生长因子-β(transforming grow factor-β, TGF-β)水平;Pearson法分析CD4~+CD25~+CD127~(low/-)Treg与CD4~+CD25~+FoxP3~+Treg,CD4~+CD25~+CD127~(low/-)Treg、CD4~+CD25~+FoxP3~+Treg与牙周袋探诊深度的相关性。结果轻中度组、重度组CD4~+CD25~+FoxP3~+Treg[(2.88±1.50)%、(2.52±1.03)%]、CD4~+CD25~+CD127~(low/-)Treg表达[(4.42±1.49)%、(3.85±1.02)%]、血浆TGF-β水平[(1 054.18±370.35)、(1 003.78±398.76)ng/L]低于对照组[(3.57±1.11)%、(6.27±1.67)%、(1 296.60±303.17)ng/L](P<0.05),轻中度组与重度组比较差异无统计学意义(P>0.05);Pearson线性相关分析结果显示,CD4~+CD25~+FoxP3~+Treg与CD4~+CD25~+CD127~(low/-)Treg表达呈正相关(r=0.776,P<0.001),CD4~+CD25~+FoxP3~+Treg、CD4~+CD25~+CD127~(low/-)Treg均与牙周袋探诊深度呈负相关(r=-0.333,P=0.005;r=-0.551,P<0.001)。结论慢性牙周炎CD4~+CD25~+Treg介导的免疫应答减弱,可能促进疾病的发生、发展。
Objective To investigate the expression of CD4~+ CD25~+ regulatory T cells(Treg) in peripheral blood of patients with chronic periodontitis(CP). Methods Forty patients with CP were divided into mild to moderated group(n=22) and severe group(n=18), and another 30 healthy volunteers were as controls(control group). The levels of CD4~+ CD25~+ CD127~(low/-) Treg and CD4~+ CD25~+ FoxP3~+Treg in peripheral blood were detected by flow cytometry in three groups. The levels of transforming growth factor-β(TGF-β) was detected by ELISA technique. The correlations of CD4~+CD25~+CD127~(low/-)Treg with CD4~+CD25~+FoxP3~+Treg, as well as CD4~+CD25~+CD127~(low/-) Treg and CD4~+CD25~+FoxP3~+Treg with periodontal probing depth were analyzed by Pearson correlation method. Results The levels of CD4~+CD25~+FoxP3~+Treg((2.88±1.50)%,(2.52±1.03)%), CD4~+CD25~+CD127~(low/-) Treg((4.42±1.49)%,(3.85±1.02)%) and TGF-β((1 054.18±370.35),(1 003.78±398.76) ng/L) in mild to moderate group and severe group were significantly lower than those in control group((3.57±1.11)%,(6.27±1.67)%,(1 296.60±303.17) ng/L)(P<0.05), and showed no significant differences between mild to moderate group and severe group(P>0.05). Pearson correlation analysis indicated that CD4~+CD25~+FoxP3~+Treg was positively with CD4~+CD25~+CD127~(low/-) Treg(r=0.776, P<0.001), while CD4~+CD25~+FoxP3~+Treg and CD4~+CD25~+CD127~(low/-) Treg were negatively correlated with periodontal probing depth(r=-0.333, P=0.005; r=-0.551, P<0.001). Conclusion CD4~+CD25~+ Treg mediated immune responses in CP are weakened and might contribute to the development and progression of CP.
Objective To investigate the expression of CD4~+ CD25~+ regulatory T cells(Treg) in peripheral blood of patients with chronic periodontitis(CP). Methods Forty patients with CP were divided into mild to moderated group(n=22) and severe group(n=18), and another 30 healthy volunteers were as controls(control group). The levels of CD4~+ CD25~+ CD127~(low/-) Treg and CD4~+ CD25~+ FoxP3~+Treg in peripheral blood were detected by flow cytometry in three groups. The levels of transforming growth factor-β(TGF-β) was detected by ELISA technique. The correlations of CD4~+CD25~+CD127~(low/-)Treg with CD4~+CD25~+FoxP3~+Treg, as well as CD4~+CD25~+CD127~(low/-) Treg and CD4~+CD25~+FoxP3~+Treg with periodontal probing depth were analyzed by Pearson correlation method. Results The levels of CD4~+CD25~+FoxP3~+Treg((2.88±1.50)%,(2.52±1.03)%), CD4~+CD25~+CD127~(low/-) Treg((4.42±1.49)%,(3.85±1.02)%) and TGF-β((1 054.18±370.35),(1 003.78±398.76) ng/L) in mild to moderate group and severe group were significantly lower than those in control group((3.57±1.11)%,(6.27±1.67)%,(1 296.60±303.17) ng/L)(P<0.05), and showed no significant differences between mild to moderate group and severe group(P>0.05). Pearson correlation analysis indicated that CD4~+CD25~+FoxP3~+Treg was positively with CD4~+CD25~+CD127~(low/-) Treg(r=0.776, P<0.001), while CD4~+CD25~+FoxP3~+Treg and CD4~+CD25~+CD127~(low/-) Treg were negatively correlated with periodontal probing depth(r=-0.333, P=0.005; r=-0.551, P<0.001). Conclusion CD4~+CD25~+ Treg mediated immune responses in CP are weakened and might contribute to the development and progression of CP.
引文
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[15] 石嘉俪,张淳.标准化屋尘螨特异性免疫治疗对变应性鼻炎患者外周血CD4+CD25+CD127low/-调节性T细胞的影响[J].中华实用诊断与治疗杂志,2015,29(3):300-302.
[16] 潘君素,许亚亚,梁军兵,等.CD4+CD25+CD127low/-Treg在慢性阻塞性肺疾病中的表达及意义[J].中华全科医学,2014,12(6):927-929.
[17] 杜毅,于西佼,唐开亮.CD4+CD25+T细胞在牙周炎发生时的免疫调节作用研究[J].牙体牙髓牙周病学杂志,2014,24(3):142-145.
[18] 张黎,张天夫,万惠,等.Th17/Treg细胞在各型慢性牙周炎中含量的比较分析[J].中国实验诊断学,2017,21(11):1965-1968.
[19] 王琳源,关宁,林晓萍.Treg 细胞介导的免疫应答在牙周炎小鼠中的研究[J].实用口腔医学杂志,2015,31(3):318-322.
[2] CHEN X T,CHEN L L,TAN J Y,et al.Cytokine levels in plasma and gingival crevicular fluid in chronic periodontitis[J].Am J Dent,2015,28(1):9-12.
[3] 童若燕,张峰,张晓红,等.Treg细胞与感染性疾病的研究进展[J].皮肤病与性病,2014,36(3):148-151.
[4] 曹采方,孟焕新,阎福华,等.牙周疾病新分类简介(1999年国际研讨会)[J].中华口腔医学杂志,2001,36(5):391-393.
[5] 李厚轩,闫福华.重度牙周炎的诊断标准和牙周炎进展的判断标准[J].中国实用口腔科杂志,2016,9(4):193-196.
[6] GERRIETS V A,KISHTON R J,JOHNSON M O,et al.FoxP3 and Toll-like receptor signaling balance Treg cell anabolic metabolism for suppression[J].Nat Immunol,2016,17(12):1459-1466.
[7] 金熙,李虹.抗原特异性调节性T细胞的研究进展及其在临床中的应用前景[J].华西医学,2018,33(5):132-138.
[8] 马金环.急性胃出血患者早期血液CD4+CD25+T细胞及其细胞因子的变化[J].中国实验诊断学,2017,21(7):1170-1172.
[9] FONTENOT J D,GAVIN M A,RUDENSKY A Y.Pillars article:FoxP3 programs the development and function of CD4+ CD25+ regulatory T cells[J].Nat Immunol,2017,198(3):986-992.
[10] KHALILI A,EBRAHIMPOUR S,MALEKI I,et al.CD4+ CD25+ CD127low FoxP3+ regulatory T cells in Crohn's disease[J].Rom J Intern Med,2018,56(3):158-166.
[11] 祝丽晶,吾慧瑛,侯盼飞.CD4+CD25+FoxP3+调节性T细胞增龄性变化及其与老年肺癌的关系[J].中华实用诊断与治疗杂志,2017,31(12):1197-1200.
[12] CIEBIADA M,KASZTALSKA K,GORSKA-CIEBIADA M,et al.Expression of IL-7 receptor in human peripheral regulatory T cells[J].Arch Med Sci,2013,9(3):555-560.
[13] MOHAMMADNIA-AFROUZI M,ZAVARAN HOSSEINI A,KHALILI A,et al.Decrease of CD4+ CD25+ CD127 low FoxP3+ regulatory T cells with impaired suppressive function in untreated ulcerative colitis patients[J].Autoimmunity,2015,48(8):556-561.
[14] YU N,LI X,SONG W,et al.CD4+ CD25+ CD127 low/- T cells:a more specific Treg population in human peripheral blood[J].Inflammation,2012,35(6):1773-1780.
[15] 石嘉俪,张淳.标准化屋尘螨特异性免疫治疗对变应性鼻炎患者外周血CD4+CD25+CD127low/-调节性T细胞的影响[J].中华实用诊断与治疗杂志,2015,29(3):300-302.
[16] 潘君素,许亚亚,梁军兵,等.CD4+CD25+CD127low/-Treg在慢性阻塞性肺疾病中的表达及意义[J].中华全科医学,2014,12(6):927-929.
[17] 杜毅,于西佼,唐开亮.CD4+CD25+T细胞在牙周炎发生时的免疫调节作用研究[J].牙体牙髓牙周病学杂志,2014,24(3):142-145.
[18] 张黎,张天夫,万惠,等.Th17/Treg细胞在各型慢性牙周炎中含量的比较分析[J].中国实验诊断学,2017,21(11):1965-1968.
[19] 王琳源,关宁,林晓萍.Treg 细胞介导的免疫应答在牙周炎小鼠中的研究[J].实用口腔医学杂志,2015,31(3):318-322.