Wnt2在奶牛胎盘发育过程中的表达与定位
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摘要
胎盘发育异常是体细胞克隆失败的主要原因之一,但其机制知之甚少,而Wnt信号通路广泛参与细胞命运决定、胎儿发育和胎盘形成等生命过程。采用qRT-PCR和原位杂交等方法检测Wnt2在奶牛胎盘中的表达与定位,发现Wnt2主要富集在子叶中并定位于子叶滋养层细胞上。结果表明,Wnt2在奶牛胎盘发育过程中发挥着重要的调节功能。为解决奶牛胎盘发育异常的问题找到新的分子作用靶点。
The placenta dysplasia is one of the main causes for the failure of the somatic cell clone,but its mechanism is poorly understood.Wnt signaling pathway is widely involved in life processes such as cell fate determination,fetal development and placental formation and so on.Real-time quantitative PCR and in situ hybridization were employed to detect the expression and localization of Wnt2 during bovine placenta and found that Wnt2 mRNA was more abundant in cotyledon tissues and located mainly in cotyledon trophoblastic cells.These results indicated that Wnt2 may play an an important regulating function during placental development in dairy cows.New molecular targets are found to solve the placenta dysplasia.
The placenta dysplasia is one of the main causes for the failure of the somatic cell clone,but its mechanism is poorly understood.Wnt signaling pathway is widely involved in life processes such as cell fate determination,fetal development and placental formation and so on.Real-time quantitative PCR and in situ hybridization were employed to detect the expression and localization of Wnt2 during bovine placenta and found that Wnt2 mRNA was more abundant in cotyledon tissues and located mainly in cotyledon trophoblastic cells.These results indicated that Wnt2 may play an an important regulating function during placental development in dairy cows.New molecular targets are found to solve the placenta dysplasia.
引文
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[10]DENICOL A C,BLOCK J,KELLEY D E,et al.The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo[J].FASEB Journal,2014,28(9):3975-3986.
[11]DENICOL A C,DOBBS K B,McLEAN K M,et al.Canonical WNT signaling regulates development of bovine embryos to the blastocyst stage[J].Scientific Reports,2013,3(7):1266.
[12]POLLHEIMER J,LOREGGER T,SONDEREGGERS,et al.Activation of the canonical wingless/t-cell factor signaling pathway promotes invasive differentiation of human trophoblast[J].American Journal of Pathology,2006,168(4):1134-1147.
[13]MARTIN K,JRGEN P.Human placental trophoblast invasion and differentiation:aparticular focus on Wnt signaling[J].Frontiers in Genetics,2013,4(4):190.
[2]KOHANGHADR H R,LEFEBVRE R C,FECTEAUG,et al.Ultrasonographic and histological characterization of the placenta of somatic nuclear transfer-derived pregnancies in dairy cattle[J].Theriogenology,2008,69(2):218-230.
[3]WANG H,DEY S K.Roadmap to embryo implantation:clues from mouse models[J].Nature Reviews Genetics,2006,7(3):185-199.
[4]MONKLEY S J,DELANEY S J,PENNISI D J,et al.Targeted disruption of the Wnt2 gene results in placentation defects[J].Development,1996,122(11):3343-3353.
[5]HABERMEHL K H.Die altersbestimmung beim hauswiederkuer[M].Berlin:Verlag Paul Parey,1975.
[6]DAS S K,WANG X N,PARIA B C,et al.Heparinbinding EGF-like growth factor gene is induced in the mouse uterus temporally by the blastocyst solely at the site of its apposition:apossible ligand for interaction with blastocyst EGF-receptor in implantation[J].Development,1994,120(5):1071-1083.
[7]LIN J,SHI L,ZHANG M,et al.Defects in Trophoblast cell lineage account for the impaired in vivo development of cloned embryos generated by somatic nuclear transfer[J].Cell Stem Cell,2011,8(4):371-375.
[8]SUZUKI Y,KOSHI K,IMAI K,et al.Bone morphogenetic protein 4accelerates the establishment of bovine trophoblastic cell lines[J].Reproduction,2011,142(5):733-743.
[9]TSAMPALAS M,GRIDELET V,BERNDT S,et al.Human chorionic gonadotropin:a hormone with immunological and angiogenic properties[J].Journal of Reproductive Immunology,2010,85(1):93-98.
[10]DENICOL A C,BLOCK J,KELLEY D E,et al.The WNT signaling antagonist Dickkopf-1 directs lineage commitment and promotes survival of the preimplantation embryo[J].FASEB Journal,2014,28(9):3975-3986.
[11]DENICOL A C,DOBBS K B,McLEAN K M,et al.Canonical WNT signaling regulates development of bovine embryos to the blastocyst stage[J].Scientific Reports,2013,3(7):1266.
[12]POLLHEIMER J,LOREGGER T,SONDEREGGERS,et al.Activation of the canonical wingless/t-cell factor signaling pathway promotes invasive differentiation of human trophoblast[J].American Journal of Pathology,2006,168(4):1134-1147.
[13]MARTIN K,JRGEN P.Human placental trophoblast invasion and differentiation:aparticular focus on Wnt signaling[J].Frontiers in Genetics,2013,4(4):190.