EMS处理对橡胶草种子萌发的影响
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摘要
为探索EMS处理对橡胶草种子萌发的影响,确定EMS诱变橡胶草种子的最适宜条件。本研究以橡胶草品系CXCH为材料,EMS为诱变剂,采用4个EMS处理浓度(0.10%、0.20%、0.30%和0.40%),3个处理时间(4、8和12 h),分别对诱变后的种子发芽率、发芽势、发芽指数、成苗率和相对成苗率进行分析。研究发现,EMS诱变处理不能显著抑制橡胶草种子发芽率,却能显著降低橡胶草种子的发芽活力(发芽势和发芽指数)和成苗情况(成苗率和相对成苗率),且EMS处理浓度对橡胶草种子的影响要大于处理时间的影响,并最终确定EMS处理浓度为0.30%、诱导时间为12 h时为橡胶草种子最适宜诱变条件。
In order to explore the effect of ethyl methyl sulfonate(EMS) treatment on seed germination of Taraxacum kok-saghyz Rodin(TKS) and optimize the conditions of EMS mutagenesis of TKS seeds,seeds of TKS line CXCH were treated with EMS at four EMS concentrations(0.10%, 0.20%, 0.30% and0.40%) for 3 different durations of time(4, 8 and 12 hours) for mutagenesis. The treated seeds were germinated and cultured into seedlings, and their germination rate, germination vigor, germination index,seedling rate and relative seedling rate were analyzed. The results showed that the EMS treatment had little effect on the germination rate of TKS seeds, but had a significant inhibitive effect on germination vigor, germination index, seedling rate and relative seedling rate, and that the concentration of EMS had a higher inhibition effect on the seeds than the duration of treatment time. The seeds treated with EMS at a concentration of 0.30% for 12 hours were optimal for EMS mutagenesis.
In order to explore the effect of ethyl methyl sulfonate(EMS) treatment on seed germination of Taraxacum kok-saghyz Rodin(TKS) and optimize the conditions of EMS mutagenesis of TKS seeds,seeds of TKS line CXCH were treated with EMS at four EMS concentrations(0.10%, 0.20%, 0.30% and0.40%) for 3 different durations of time(4, 8 and 12 hours) for mutagenesis. The treated seeds were germinated and cultured into seedlings, and their germination rate, germination vigor, germination index,seedling rate and relative seedling rate were analyzed. The results showed that the EMS treatment had little effect on the germination rate of TKS seeds, but had a significant inhibitive effect on germination vigor, germination index, seedling rate and relative seedling rate, and that the concentration of EMS had a higher inhibition effect on the seeds than the duration of treatment time. The seeds treated with EMS at a concentration of 0.30% for 12 hours were optimal for EMS mutagenesis.
引文
[1]林伯煌,魏小弟.橡胶草的研究进展[J].安徽农业科学,2009,37(13):5 950-5 951.
[2]罗士苇,吴相钰,冯午.橡胶草的研究部分II-新疆产橡胶草的化学分析及其橡胶含量之测定[J].中国科学,1951,2(3):381-387.
[3]Van Beilen J B,Poirier Y.Establishment of new crops for the production of natural rubber[J].Trends Biotechnol,2007,25(11):522-529.
[4]仇建,张继川,罗世巧,等.橡胶草的研究进展[J].植物学报,2015,50(1):133-141.
[5]赵平娟,安锋,林位夫,等.大力开展巴西橡胶树替代产胶植物及技术研发的建议[J].中国农学通报,2012,28(34):124-130.
[6]Greene E A,Codomo C A,Taylor N E.et al.Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis[J].Genetics,2003,164(2):731-740.
[7]樊双虎,郭文柱,路小铎,等.玉米EMS突变体库构建及突变体初步鉴定[J].安徽农业科学,2014(11):3 162-3 165,3 185.
[8]石海春,谭义川,夏伟,等.19份玉米EMS诱变系的遗传差异评价[J].华北农学报,2016,31(1):110-116.
[9]王峰,徐飚,杨正林,等.EMS诱变水稻矮生资源的鉴定评价[J].核农学报,2011,25(2):197-201.
[10]薛芳,褚洪雷,胡志伟,等.EMS对新春11小麦抗性淀粉和农艺性状的诱变效果[J].麦类作物学报,2010,30(3):431-434.
[11]张纪元,张平平,姚金保,等.以EMS诱变创制软质小麦宁麦9号高分子量谷蛋白亚基突变体[J].作物学报,2014,40(9):1 579-1 584.
[12]Caldwell D G,Mccallum N,Shaw P,et al.A structured mutant population for forward and reverse genetics in Barley(Hordeum vulgare L.)[J].The Plant Journal,2004,40(1):143-150.
[13]Cooper J L,Till B J,Laport R G,et al.TILLING to detect induced mutations in soybean[J].BMC Plant Biology,2008,8(8):9.
[14]吴秀红.EMS诱发大豆不同品种M2代与M3代农艺性状变异比较[J].中国农学通报,2012,28(27):49-52.
[15]殷冬梅,杨秋云,杨海棠,等.花生突变体的EMS诱变及分子检测[J].中国农学通报,2009,25(5):53-56.
[16]曲高平,孙妍妍,庞红喜,等.甘蓝型油菜EMS突变体库构建及抗除草剂突变体筛选[J].中国油料作物学报,2014(1):25-31.
[17]Menda N,Semel Y,Peled D,et al.In silico screening of a saturated mutation library of tomato[J].The Plant Journal,2004,38(5):861-872.
[18]杨建华,崔霞,常培培,等.EMS诱变番茄自交系TTD302A的突变表型鉴定和分析[J].中国蔬菜,2014,1(4):21-28.
[19]Wang J G,Guo Y,Che D D,et al.RAPD analysis of M1generation of Gladiolus hybridus Hort treated by EMS[J].Journal of Northeast Agricultural University:English Edition,2009(1):5-9.
[20]梅凌锋,唐艳梅,唐晓敏,等.3种化学诱变剂对广金钱草种子萌发的影响[J].广东药学院学报,2015,31(3):310-315.
[21]吴兴兰,孙超,燕丽萍,等.EMS对紫花苜蓿中苜一号种子萌发和生长的影响[J].山东林业科技,2015,2:1-3.
[22]卢银,刘梦洋,王彦华,等.EMS处理对大白菜种子萌发及主要生化指标的影响[J].中国蔬菜,11:20-24.
[23]马海新,庞胜群,杨邦杰,等.EMS诱变对加工番茄种子萌发的影响[J].种子,2015,34(5):28-33.
[24]Lin T,Xu X,Ruan J,et al.Genome analysis of Taraxacum kok-saghyz Rodin provides new insights into rubber biosynthesis[J].National Science Review,2018,5:78-87.
[25]Kim Y,Schumaker K S,Zhu J K.EMS mutagenesis of Arabidopsis[J].Methods in Molecular Biology,2006,323:101-103.
[26]刘威,杨峰,任旭东,等.EMS对种子萌发影响的进展[J].分子植物育种,2017,15(11):4 585-4 589.
[27]Liu J X.Effects of induced concentration and time of EMS on seed germination of common Buckwheat[J].Agricultural Science&Technology,2015,16(10):2 081-2 083.
[28]邢飞,王晓雪,任旭东,等.EMS诱变技术在水稻育种中的应用[J].南方农业,2016,06(10):247-249.
[29]曾新华.不同诱变方法对油菜种子诱变效果及突变体的研究[D].武汉:华中农业大学,2010.
[30]胡志峰,魏臻武,唐嘉.EMS诱变处理金花菜种子条件分析[J].草地学报,2017,25(2):361-365.
[31]王学征,朱娜娜,高清宇,等.EMS诱变西瓜种子条件分析[J].东北农业大学学报,2015,46(7):35-39.
[32]袁小环,武菊英,杨学军,等.基于半致死浓度的观赏草萌发期和幼苗期耐盐性评价[J].中国草地学报,2012(6):49-53.
[2]罗士苇,吴相钰,冯午.橡胶草的研究部分II-新疆产橡胶草的化学分析及其橡胶含量之测定[J].中国科学,1951,2(3):381-387.
[3]Van Beilen J B,Poirier Y.Establishment of new crops for the production of natural rubber[J].Trends Biotechnol,2007,25(11):522-529.
[4]仇建,张继川,罗世巧,等.橡胶草的研究进展[J].植物学报,2015,50(1):133-141.
[5]赵平娟,安锋,林位夫,等.大力开展巴西橡胶树替代产胶植物及技术研发的建议[J].中国农学通报,2012,28(34):124-130.
[6]Greene E A,Codomo C A,Taylor N E.et al.Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis[J].Genetics,2003,164(2):731-740.
[7]樊双虎,郭文柱,路小铎,等.玉米EMS突变体库构建及突变体初步鉴定[J].安徽农业科学,2014(11):3 162-3 165,3 185.
[8]石海春,谭义川,夏伟,等.19份玉米EMS诱变系的遗传差异评价[J].华北农学报,2016,31(1):110-116.
[9]王峰,徐飚,杨正林,等.EMS诱变水稻矮生资源的鉴定评价[J].核农学报,2011,25(2):197-201.
[10]薛芳,褚洪雷,胡志伟,等.EMS对新春11小麦抗性淀粉和农艺性状的诱变效果[J].麦类作物学报,2010,30(3):431-434.
[11]张纪元,张平平,姚金保,等.以EMS诱变创制软质小麦宁麦9号高分子量谷蛋白亚基突变体[J].作物学报,2014,40(9):1 579-1 584.
[12]Caldwell D G,Mccallum N,Shaw P,et al.A structured mutant population for forward and reverse genetics in Barley(Hordeum vulgare L.)[J].The Plant Journal,2004,40(1):143-150.
[13]Cooper J L,Till B J,Laport R G,et al.TILLING to detect induced mutations in soybean[J].BMC Plant Biology,2008,8(8):9.
[14]吴秀红.EMS诱发大豆不同品种M2代与M3代农艺性状变异比较[J].中国农学通报,2012,28(27):49-52.
[15]殷冬梅,杨秋云,杨海棠,等.花生突变体的EMS诱变及分子检测[J].中国农学通报,2009,25(5):53-56.
[16]曲高平,孙妍妍,庞红喜,等.甘蓝型油菜EMS突变体库构建及抗除草剂突变体筛选[J].中国油料作物学报,2014(1):25-31.
[17]Menda N,Semel Y,Peled D,et al.In silico screening of a saturated mutation library of tomato[J].The Plant Journal,2004,38(5):861-872.
[18]杨建华,崔霞,常培培,等.EMS诱变番茄自交系TTD302A的突变表型鉴定和分析[J].中国蔬菜,2014,1(4):21-28.
[19]Wang J G,Guo Y,Che D D,et al.RAPD analysis of M1generation of Gladiolus hybridus Hort treated by EMS[J].Journal of Northeast Agricultural University:English Edition,2009(1):5-9.
[20]梅凌锋,唐艳梅,唐晓敏,等.3种化学诱变剂对广金钱草种子萌发的影响[J].广东药学院学报,2015,31(3):310-315.
[21]吴兴兰,孙超,燕丽萍,等.EMS对紫花苜蓿中苜一号种子萌发和生长的影响[J].山东林业科技,2015,2:1-3.
[22]卢银,刘梦洋,王彦华,等.EMS处理对大白菜种子萌发及主要生化指标的影响[J].中国蔬菜,11:20-24.
[23]马海新,庞胜群,杨邦杰,等.EMS诱变对加工番茄种子萌发的影响[J].种子,2015,34(5):28-33.
[24]Lin T,Xu X,Ruan J,et al.Genome analysis of Taraxacum kok-saghyz Rodin provides new insights into rubber biosynthesis[J].National Science Review,2018,5:78-87.
[25]Kim Y,Schumaker K S,Zhu J K.EMS mutagenesis of Arabidopsis[J].Methods in Molecular Biology,2006,323:101-103.
[26]刘威,杨峰,任旭东,等.EMS对种子萌发影响的进展[J].分子植物育种,2017,15(11):4 585-4 589.
[27]Liu J X.Effects of induced concentration and time of EMS on seed germination of common Buckwheat[J].Agricultural Science&Technology,2015,16(10):2 081-2 083.
[28]邢飞,王晓雪,任旭东,等.EMS诱变技术在水稻育种中的应用[J].南方农业,2016,06(10):247-249.
[29]曾新华.不同诱变方法对油菜种子诱变效果及突变体的研究[D].武汉:华中农业大学,2010.
[30]胡志峰,魏臻武,唐嘉.EMS诱变处理金花菜种子条件分析[J].草地学报,2017,25(2):361-365.
[31]王学征,朱娜娜,高清宇,等.EMS诱变西瓜种子条件分析[J].东北农业大学学报,2015,46(7):35-39.
[32]袁小环,武菊英,杨学军,等.基于半致死浓度的观赏草萌发期和幼苗期耐盐性评价[J].中国草地学报,2012(6):49-53.