驼血中降脂多肽的分离
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摘要
以骆驼血为原料,经过超声波破碎、透析等预处理后,样品在胃蛋白酶和胰蛋白酶共同作用以及底物浓度为5%,酶添加量为5%,最适温度和最适pH酶解4 h的条件下,样品中大分子的蛋白质可以较好地被水解成小分子多肽。使用不同分子质量的超滤管将酶解液分为大于10ku、3～10 ku、小于3 ku 3个组分,分别测定其HMG﹣CoA还原酶抑制率。结果表明:酶解液分子质量在3～10 ku范围内具有较高的HMG﹣CoA还原酶抑制率,其HMG﹣CoA还原酶抑制率为11%,其抑制率是市面已售降脂药Pravachol的4倍。
The study used camel blood as a raw material. After pretreatment with ultrasonication,dialysis, etc., the sample was co-administered with pepsin and trypsin at substrate concentration of 5%,enzyme addition of 5%, optimum temperature and optimum pH, and enzymatic hydrolysis for 4 h. The macromolecular protein in the sample can be better hydrolyzed into a small molecule polypeptide. The enzymatic hydrolysates were divided into three components of greater than 10 ku, 3～10 ku and less than 3 ku using different molecular weight ultrafiltration tubes, and the HMG-CoA reductase inhibition rate was measured. The results showed that the molecular weight of the hydrolysate in the range of 3～10 ku had a higher inhibition rate of HMG-CoA reductase, and the inhibition rate of HMG-CoA reductase was 11%.The inhibition rate was the commercially available lipid-lowering drug Pravachol 4 times.
The study used camel blood as a raw material. After pretreatment with ultrasonication,dialysis, etc., the sample was co-administered with pepsin and trypsin at substrate concentration of 5%,enzyme addition of 5%, optimum temperature and optimum pH, and enzymatic hydrolysis for 4 h. The macromolecular protein in the sample can be better hydrolyzed into a small molecule polypeptide. The enzymatic hydrolysates were divided into three components of greater than 10 ku, 3～10 ku and less than 3 ku using different molecular weight ultrafiltration tubes, and the HMG-CoA reductase inhibition rate was measured. The results showed that the molecular weight of the hydrolysate in the range of 3～10 ku had a higher inhibition rate of HMG-CoA reductase, and the inhibition rate of HMG-CoA reductase was 11%.The inhibition rate was the commercially available lipid-lowering drug Pravachol 4 times.
引文
[1]Shuangquan,Burentegusi,Miyamoto T.Microflora in traditional fermented camel’s milk from Inner Mongolia,China[J].Milchwissenschaft﹣milk Science International,2004,59(11):649﹣652.
[2]火婷,刘永峰,刘毅,等.驼血的研究进展[J].草原与草业,2016,28(02):23﹣27.
[3]钟耀广,南庆贤.国内外畜血研究动态[J].中国农业科技导报,2003,(03):26﹣29.
[4]杨闯.生物活性肽在营养保健中的应用[J].食品科学,2003,24(11):153.
[5]苏秀兰.生物活性肽的研究进展[J].内蒙古医学院学报,2006,28(05):471.
[6]董海英,王海滨.鸭血、鸭骨氨基酸分析与评价[J].肉类工业,2009,(09):24﹣26.
[7]崔凤霞.海参胶原蛋白生化性质及胶原肽活性研究[D].青岛:中国海洋大学,2007.
[8]KADIMI T,MAHGOUB O,PURCHAS RW.A review of the growth,and of the carcass and meat quality characteristics of the one﹣humped camel(Camelus dromedaries)[J].Meat Science,2008,80:555﹣569.
[9]刘毅,刘永峰,王宁丽,等.驼血多肽氨基酸成分分析[J].食品工业,2017,38(08):294﹣296.
[10]邓苙,陈静,杨通念,等.血脂异常的危险因素研究进展[J].养生保健指南,2017,(9):1﹣12.
[11]The synthesis of cholesterol[J].Journal of Lipid Re﹣search,2015,56(2):319﹣330.
[12]鲁云风,文祯中,原国辉,等.3种血液抗凝剂的抗凝效果及对RAPD反应影响的研究[J].南阳师范学院学报(自然科学版),2004,(12):55﹣57.
[13]丁静雨.不同强度超声波促进酶解污泥提取蛋白质的研究[D].郑州:郑州大学,2018.
[14]黄轶,华超,徐江英,等.透析袋电洗脱法在蛋白质回收和纯化中的应用[J].南京军医学院学报,2003,(01):33﹣34.
[15]邓多.BCA法测定Sprague﹣Dawley大鼠肝微粒体蛋白浓度[A].2010年广东省药师周大会论文集[C].广州:广东省药学会,2011:1.
[16]Tagliazucchi D,Martini S,Shamsia S,et al.Biological activities and peptidomic profile of in vitro﹣digested cow,camel,goat and sheep milk[J].International Dairy Journal,2018,81.
[17]刘永峰,刘毅,徐大泽,等.响应曲面法优化驼血多肽酶解工艺研究[J].食品工业,2017,38(03):152﹣156.
[18]罗艳华,王全杰,陈沛海,等.蛋白水解物水解度测定方法的研究[J].皮革与化工,2017,34(02):26﹣31.
[19]马成,张琪,潘一廷,等.基于超滤辅助样品制备方法的优化[J].生物技术通讯,2014,25(01):82﹣86.
[20]王晶,王晴,孙吉叶,等.血脂康抑制HMG﹣CoA还原酶活性的定量测定研究[J].中国新药杂志,2015,24(16):1825﹣1830.
[2]火婷,刘永峰,刘毅,等.驼血的研究进展[J].草原与草业,2016,28(02):23﹣27.
[3]钟耀广,南庆贤.国内外畜血研究动态[J].中国农业科技导报,2003,(03):26﹣29.
[4]杨闯.生物活性肽在营养保健中的应用[J].食品科学,2003,24(11):153.
[5]苏秀兰.生物活性肽的研究进展[J].内蒙古医学院学报,2006,28(05):471.
[6]董海英,王海滨.鸭血、鸭骨氨基酸分析与评价[J].肉类工业,2009,(09):24﹣26.
[7]崔凤霞.海参胶原蛋白生化性质及胶原肽活性研究[D].青岛:中国海洋大学,2007.
[8]KADIMI T,MAHGOUB O,PURCHAS RW.A review of the growth,and of the carcass and meat quality characteristics of the one﹣humped camel(Camelus dromedaries)[J].Meat Science,2008,80:555﹣569.
[9]刘毅,刘永峰,王宁丽,等.驼血多肽氨基酸成分分析[J].食品工业,2017,38(08):294﹣296.
[10]邓苙,陈静,杨通念,等.血脂异常的危险因素研究进展[J].养生保健指南,2017,(9):1﹣12.
[11]The synthesis of cholesterol[J].Journal of Lipid Re﹣search,2015,56(2):319﹣330.
[12]鲁云风,文祯中,原国辉,等.3种血液抗凝剂的抗凝效果及对RAPD反应影响的研究[J].南阳师范学院学报(自然科学版),2004,(12):55﹣57.
[13]丁静雨.不同强度超声波促进酶解污泥提取蛋白质的研究[D].郑州:郑州大学,2018.
[14]黄轶,华超,徐江英,等.透析袋电洗脱法在蛋白质回收和纯化中的应用[J].南京军医学院学报,2003,(01):33﹣34.
[15]邓多.BCA法测定Sprague﹣Dawley大鼠肝微粒体蛋白浓度[A].2010年广东省药师周大会论文集[C].广州:广东省药学会,2011:1.
[16]Tagliazucchi D,Martini S,Shamsia S,et al.Biological activities and peptidomic profile of in vitro﹣digested cow,camel,goat and sheep milk[J].International Dairy Journal,2018,81.
[17]刘永峰,刘毅,徐大泽,等.响应曲面法优化驼血多肽酶解工艺研究[J].食品工业,2017,38(03):152﹣156.
[18]罗艳华,王全杰,陈沛海,等.蛋白水解物水解度测定方法的研究[J].皮革与化工,2017,34(02):26﹣31.
[19]马成,张琪,潘一廷,等.基于超滤辅助样品制备方法的优化[J].生物技术通讯,2014,25(01):82﹣86.
[20]王晶,王晴,孙吉叶,等.血脂康抑制HMG﹣CoA还原酶活性的定量测定研究[J].中国新药杂志,2015,24(16):1825﹣1830.