静原鸡ELOVL2基因内含子4多态性分析
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摘要
为了明确静原鸡ELOVL2基因的遗传特性,试验采用PCR-SSCP技术对静原鸡ELOVL2基因内含子4遗传多样性进行检测。结果表明:在ELOVL2基因内含子4扩增片段上共检测到4种基因型、3种等位基因、2个SNPs位点。其中A和B为优势等位基因(0.47),基因型AB为优势基因型(0.42);静原鸡ELOVL2基因内含子4遗传纯合度与杂合度基本相同,且偏离了Hardy-Weinberg平衡(P<0.01)。说明静原鸡ELOVL2基因内含子4序列突变程度较高且呈中度多态,杂合子或纯合子无明显优势。
引文
[1]顾亚玲,张娟,何生虎.静原鸡品种选育与饲养管理[M].银川:阳光出版社,2017.
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[4]GUILLOU H,ZADRAVEC D,MARTIN P G,et al.The key roles of elongases and desaturases in mammalian fatty acid metabolism:Insights from transgenic mice[J].Prog Lipid Res,2010,49(2):186-199.
[5]LEONARD A E,KELDER B,BOBIK E G,et al.Identification and expression of mammalian long-chain PUFA elongation enzymes[J].Lipids,2002,37(8):733-740.
[6]LEMAITRE R N,TANAKA T,TANG W,et al.Genetic loci associated with plasma phospholipid n-3 fatty acids:a meta-analysis of genome-wide association studies from the CHARGE Consortium.[J].PLo S Genet,2011(7):e1002193.
[7]孙晓艳.小鼠elovl2与△6 desaturase及线虫sfat1三基因共转促进EPA向DHA转化的研究[D].西安:陕西师范大学,2012.
[8]母童,张娟,赵平,等.静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究[J].浙江农业学报,2017(8):1290-1296.
[9]夏德佳,孙琳,史杰萍,等.多不饱和脂肪酸代谢限速酶基因多态性与吉林省汉族偏执型精神分裂症的病例对照研究[J].卫生研究,2011,40(2):154-157.
[10]邢华苑,王颜刚.ELOVL6基因rs9997926位点多态性与2型糖尿病病人胰岛素抵抗的相关性[J].青岛大学医学院学报,2017,53(1):84-86.
[11]LI X,GAN Z W,DING Z,et al.Genetic variants in the ELOVL5but not ELOVL2 gene associated with polyunsaturated fatty acids in han Chinese breast milk[J].Biomed Environ Sci,2017,30(1):64-67.
[2]ZHANG J,ZHAO S,LEI Z,et al.Identification of polymorphisms in the bovine paraoxonase 1 gene[J].J Anim Vet Adv,2013,12(2):229-233.
[3]NAGANUMA T,SATO Y,SASSA T,et al.Biochemical characterization of the very long-chain fatty acid elongase ELOVL7[J].Febs Lett,2011,585(20),3337-3341.
[4]GUILLOU H,ZADRAVEC D,MARTIN P G,et al.The key roles of elongases and desaturases in mammalian fatty acid metabolism:Insights from transgenic mice[J].Prog Lipid Res,2010,49(2):186-199.
[5]LEONARD A E,KELDER B,BOBIK E G,et al.Identification and expression of mammalian long-chain PUFA elongation enzymes[J].Lipids,2002,37(8):733-740.
[6]LEMAITRE R N,TANAKA T,TANG W,et al.Genetic loci associated with plasma phospholipid n-3 fatty acids:a meta-analysis of genome-wide association studies from the CHARGE Consortium.[J].PLo S Genet,2011(7):e1002193.
[7]孙晓艳.小鼠elovl2与△6 desaturase及线虫sfat1三基因共转促进EPA向DHA转化的研究[D].西安:陕西师范大学,2012.
[8]母童,张娟,赵平,等.静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究[J].浙江农业学报,2017(8):1290-1296.
[9]夏德佳,孙琳,史杰萍,等.多不饱和脂肪酸代谢限速酶基因多态性与吉林省汉族偏执型精神分裂症的病例对照研究[J].卫生研究,2011,40(2):154-157.
[10]邢华苑,王颜刚.ELOVL6基因rs9997926位点多态性与2型糖尿病病人胰岛素抵抗的相关性[J].青岛大学医学院学报,2017,53(1):84-86.
[11]LI X,GAN Z W,DING Z,et al.Genetic variants in the ELOVL5but not ELOVL2 gene associated with polyunsaturated fatty acids in han Chinese breast milk[J].Biomed Environ Sci,2017,30(1):64-67.