韩国野山参对大鼠的亚慢性毒性试验
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摘要
目的通过90 d喂养试验观察韩国野山参提取物对大鼠的亚慢性毒性,为进一步开发野山参的药用及食用价值提供依据。方法 SD大鼠80只,体重60~90 g,随机分为对照组和野山参提取物低、中、高剂量组(2.66 g/kg·BW、5.33g/kg·BW和8.00 g/kg·BW),拌饲给予,每组20只,雌雄各半,连续90 d。每周观察大鼠的体重和进食量,实验中期测定血常规,实验结束时测定血常规和血生化、解剖脏器并计算脏器系数和进行病理学检查。结果结果提示韩国野山参提取物对大鼠体重、进食量、食物利用率、病理组织学指标均未产生明显影响,试验结束时,中剂量组雄鼠嗜酸性细胞百分比高于对照组,雌雄大鼠的总蛋白及白蛋白水平高于对照组,其余血液学指标均无显著变化。结论韩国野山参提取物未见明显的亚慢性毒性损害。
Objective To observe the sub-chronic toxicity of wild ginseng extracts to rats by feeding test for 90 days,and to provide basis for further developing wild ginseng as medicine and health food. Methods Eighty SD rats weighing from 60 g to 90 g were randomly divided into control group and low,medium and high dose groups according to the dosage of wild ginseng extracts( 2. 66 g/kg·BW,5. 33 g/kg·BW and 8. 00 g/kg ·BW),given by gavage,each group with 20 rats with half male and half female,lasted for 90 days. The rats' weight and food intake of each group were observed weekly. Hemocytology test was done in the middle of the experiment. The blood and blood biochemistry of the rats in each group were examined at the end of the experiment. At the end of the experiment, the organ coefficients were calculated and the pathological examination of the organs of the rats was performed. Results Ninety days feeding test showed that wild ginseng extracts had no significant impact on the rats ' weight,food intake,food utilization and histopathological parameters in experimental rats. At the end of the experiment,the eosinophil of male rats in the medium dose group was higher than that in the control group. The total protein and albumin levels of male and female rats in the medium dose group were higher than that in control group. No significant changes were observed in other hematological parameters. Conclusion South Korea wild ginseng extracts without obvious sub-chronic toxicity on the experimental rats.
Objective To observe the sub-chronic toxicity of wild ginseng extracts to rats by feeding test for 90 days,and to provide basis for further developing wild ginseng as medicine and health food. Methods Eighty SD rats weighing from 60 g to 90 g were randomly divided into control group and low,medium and high dose groups according to the dosage of wild ginseng extracts( 2. 66 g/kg·BW,5. 33 g/kg·BW and 8. 00 g/kg ·BW),given by gavage,each group with 20 rats with half male and half female,lasted for 90 days. The rats' weight and food intake of each group were observed weekly. Hemocytology test was done in the middle of the experiment. The blood and blood biochemistry of the rats in each group were examined at the end of the experiment. At the end of the experiment, the organ coefficients were calculated and the pathological examination of the organs of the rats was performed. Results Ninety days feeding test showed that wild ginseng extracts had no significant impact on the rats ' weight,food intake,food utilization and histopathological parameters in experimental rats. At the end of the experiment,the eosinophil of male rats in the medium dose group was higher than that in the control group. The total protein and albumin levels of male and female rats in the medium dose group were higher than that in control group. No significant changes were observed in other hematological parameters. Conclusion South Korea wild ginseng extracts without obvious sub-chronic toxicity on the experimental rats.
引文
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[6]邱雪,洪铁,孟勤,等.人参皂苷单体Rb1、Re及Rg1对肾上腺素所致小鼠耳廓微循环障碍的改善作用[J].吉林大学学报(医学版),2009,35(2):314-317.
[7]胡同童,陈立,李伟,等.鲜人参与生晒参对高脂饮食和STZ诱导的2型糖尿病大鼠的降血糖作用[J].人参研究,2014,26(2):16-19.
[8]Giannakis IM,Chen SL,Karam SM,et al,Helicobacter phlori evolution during progression from chroncic atrophic gastritis to gastric cancer and its impact on gastric stem cells[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(11):4358-4363.
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[10]府伟灵.临床生物化学检验[M].5版.北京:人民卫生出版社,2012:1-1.
[11]王逸,鲍勇刚,贾韦国,等.人参蛋白研究进展[J].中草药,2013,44(19):2782-2786.
[12]Kuo YH,Lkegami F,lambein F.Neuroactive and other free amino acids in seed and young plants of Panax ginseng[J].phytochemsitry,2003,62:1087-1091.
[2]李世洋,王爽.野山参的来源、形态、产地、类别及鉴别[J].亚太传统医药,2008,4(1):37-39.
[3]董亚娟,张文驹,陈家宽,等.人参年龄的研究进展[J].中草药,2007,38(3):467-470.
[4]郭秀丽,高淑莲.人参化学成分和药理研究进展[J].中医临床研究,2012,4(14):26-27.
[5]姚根兰,张娅萍,欧阳柳凤,等.人参抗疲劳作用的研究进展[J].世界中西医结合杂志,2015,10(8):1174-1177.
[6]邱雪,洪铁,孟勤,等.人参皂苷单体Rb1、Re及Rg1对肾上腺素所致小鼠耳廓微循环障碍的改善作用[J].吉林大学学报(医学版),2009,35(2):314-317.
[7]胡同童,陈立,李伟,等.鲜人参与生晒参对高脂饮食和STZ诱导的2型糖尿病大鼠的降血糖作用[J].人参研究,2014,26(2):16-19.
[8]Giannakis IM,Chen SL,Karam SM,et al,Helicobacter phlori evolution during progression from chroncic atrophic gastritis to gastric cancer and its impact on gastric stem cells[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(11):4358-4363.
[9]刘科亮,郑华,高俊,等.2种取血方式下SD大鼠血常规及生化指标变化的探讨[J].预防医学情报杂志,2016,32(4):383-386.
[10]府伟灵.临床生物化学检验[M].5版.北京:人民卫生出版社,2012:1-1.
[11]王逸,鲍勇刚,贾韦国,等.人参蛋白研究进展[J].中草药,2013,44(19):2782-2786.
[12]Kuo YH,Lkegami F,lambein F.Neuroactive and other free amino acids in seed and young plants of Panax ginseng[J].phytochemsitry,2003,62:1087-1091.