人参皂苷分析测定方法的研究进展
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摘要
综述近年人参皂苷分析测定方法的研究新进展。人参皂苷有多种分析测定方法,主要有比色法、高效液相色谱法、超高效液相色谱法、高效液相色谱-串联质谱联用法、超高效液相色谱-串联质谱联用法及胶束电动毛细管色谱法等。对以上几种方法在检测人参皂苷类成分中的具体应用分别进行了介绍,并在此基础上进行了总结与展望。
This paper reviews the new research progress on the analytic determination methods of ginsenoside in recent years. There are many analytic determination methods of ginsenosides,mainly including colorimetry,high performance liquid chromatography,ultra high performance liquid chromatography,high performance liquid chromatography-tandem mass spectrometry,ultra high performance liquid chromatography-tandem mass spectrometry and micellar electrokinetic capillary chromatography. The specific application of the above methods in the determination of ginsenosides are introduced respectively,and on this basis,the summary and prospect are carried out.
This paper reviews the new research progress on the analytic determination methods of ginsenoside in recent years. There are many analytic determination methods of ginsenosides,mainly including colorimetry,high performance liquid chromatography,ultra high performance liquid chromatography,high performance liquid chromatography-tandem mass spectrometry,ultra high performance liquid chromatography-tandem mass spectrometry and micellar electrokinetic capillary chromatography. The specific application of the above methods in the determination of ginsenosides are introduced respectively,and on this basis,the summary and prospect are carried out.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015:8-9.
[2]赵莉,郜玉钢,姬庆.人参化学成分的免疫作用及其机制的研究进展[J].中南药学,2015,13(7):741-745.
[3]林美妤,刘海宇,刘金平,等.人参皂苷的化学研究进展[J].人参研究,2011,23(4):43-46.
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[6]万玉华,戴洁,黄春静,等.紫外分光光度法测定参须中人参总皂苷的含量[J].中国中医药现代远程教育,2012,10(24):146-147.
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[8]王磊,孙德水,郑东梅.人参糖果中人参总皂苷含量的测定[J].人参研究,2013,25(4):28-30.
[9]庞威,赵宏峰,弥宏,等.紫外-可见分光光度法测定振源口服液(无糖型)中人参总皂苷的含量[J].中国医药指南,2014,29(12):59-60.
[10]张连龙,周华生,成恒嵩.测定参类保健食品中总皂苷的新方法[J].中国食品卫生杂志,2011,23(5):442-445.
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[13]刘志,阮长春,刘天志,等.HPLC法同时测定林下参、鲜人参、生晒参和红参中14种人参皂苷[J].中草药,2012,43(12):2431-2434.
[14]樊红秀,刘婷婷,刘鸿铖,等.超临界萃取人参皂苷及HPLC分析[J].食品科学,2013,34(20):121-126.
[15]袁斌,车金水,金燕,等.快速溶剂萃取-液相色谱法测定人参药材中人参皂苷的含量[J].药物分析杂志,2013,33(7):1267-1270.
[16]郜玉钢,郝建勋,臧埔,等.高效液相色谱法测定农田人参中9种人参皂苷单体含量[J].食品科学,2012,33(2):189-193.
[17]杨艳文,孟凡双,郜玉钢,等.高效液相色谱法同时测定人参制剂中20种人参皂苷方法的建立[J].食品科学,2016,37(22):131-135.
[18]张金秋,夏娟,孙光芝,等.RP-HPLC法测定人参花中11个人参皂苷含量[J].药物分析杂志,2015,35(9):1596-1600.
[19]曾露露,赵婷,李莹,等.高效液相色谱法同时测定黑参中4种稀有人参皂苷的含量[J].中国药学杂志,2017,52(12):1069-1072.
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[22]SUN B S,GU L J,FANG Z M,et al.Simultaneous quantification of 19ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD[J].J Pharm Biomed Anal,2009,50(1):15-22.
[23]LEE S I,KWON H J,LEE Y M,et al.Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD[J].J Pharm Biomed Anal,2012(60):80-85.
[24]崔勇,李青,刘思洁,等.固相萃取-超高效液相色谱法同时测定人参中11种人参皂苷的含量[J].中国卫生检验杂志,2012,22(3):475-477.
[25]PARK H W,IN G,HAN S T,et al.Simultaneous determination of 30ginsenosides in Panax ginseng preparations using ultra performance liquid chromatography[J].J Ginseng Res,2013,37(4):457-467.
[26]逄世峰,孙成贺,李亚丽,等.MCI前处理-UPLC法测定西洋参茎叶中7种人参皂苷含量[J].特产研究,2014(1):49-51.
[27]张晓旭,马雪涛,胡蒙,等.超高效液相色谱法检测6种人参皂苷含量[J].中国食品学报,2015,15(5):241-246.
[28]张玉婷,冯克然,曹进,等.UPLC法评价多种人参提取物中人参皂苷的含量[J].食品科学,2013,34(24):102-105.
[29]李薇,吉利娜,宋新波.UPLC法测定人参流动浸膏中8种人参皂苷类成分[J].天津中医药,2014,31(10):624-626.
[30]蔡艳,宋剑,王贵金,等.UPLC法测定人参总皂苷提取物中7种人参皂苷的含量[J].西北药学杂志,2016,31(5):441-444.
[31]刘婷,徐虹.超高效液相色谱法快速测定参麦注射液中6种皂苷类成分含量[J].医药导报,2016,35(11):1259-1261.
[32]孔宏伟,王梅,Rob H J,等.HPLC/LTQ-FTMS用于白参中的丙二酰基人参皂苷的研究[J].世界科学技术-中医药现代化,2009,11(1):190-194.
[33]孙海,张亚玉,王英平,等.利用HPLC/MS对野山参和林下参皂苷的分析[J].特产研究,2011,33(3):52-55.
[34]马浩然,程璐,王富乾,等.HPLC-MS/MS法测定10批参须中人参皂苷Rg1、Re和Rb1的含量[J].中南药学,2017,15(6):807-810.
[35]STAVRIANIDI A,STEKOLSHCHIKOVA E,POROTOVA A,et al.Combination of HPLC–MS and QAMS as a new analytical approach fordetermination of saponins in ginseng containing products[J].J Pharm Biomed Anal,2017(132):87-92.
[36]陈洪岩,姚晨旭,张小龙.高效液相色谱四级杆飞行时间串联质谱法测定启脾丸中7种人参皂苷的含量[J].中国药师,2017,20(3):460-462.
[37]Li L,Luo G A,Liang Q L,et al.Rapid qualitative and quantitative analyses of Asian ginseng in adulterated American ginseng preparations by UPLC/Q-TOF-MS[J].J Pharm Biomed Anal,2010,52(1):66-72.
[38]Zhang H M,Li S L,Zhang H,et al.Holistic quality evaluation of commercial white and red ginseng using a UPLC-QTOF-MS/MS-based metabolomics approach[J].J Pharm Biomed Anal,2012,62(62):258-273.
[39]张晓旭,王洪平,杨阳,等.超高效液相色谱-串联四极杆飞行时间质谱法快速鉴别生晒参中的皂苷类成分[J].中国医药导报,2015,12(9):130-136.
[40]WANG H P,ZHANG Y B,YANG X W,et al.Rapid characterization of ginsenosides in the roots and rhizomes of Panax ginseng by UPLC-DADQTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS[J].J Ginseng Res,2016,40(4):382-394.
[41]CHEN Y J,ZHAO Z Z,CHEN H B,et al.Determination of ginsenosides in Asian and American ginsengs by liquid chromatography-quadrupole/time-of-flight MS:assessing variations based on morphological characteristics[J].J Ginseng Res,2017,41(1):10-22.
[42]钟薇,戴雨霖,李晓宇,等.超高效液相色谱-四极杆-静电场轨道阱质谱法分析不同炮制红参的化学成分差异[J].质谱学报,2015,36(6):529-534.
[43]张勇,黄鑫,李帅坪,等.UPLC Orbitrap HRMS法分析西洋参蒸参弃液浓缩物中皂苷类成分[J].质谱学报,2017,38(1):52-59.
[44]王淑美,王瑞,梁生旺,等.胶束电动毛细管色谱法分离分析人参中人参皂苷Rg1和人参皂苷Re[J].中药材,2009,32(7):1069-1071.
[45]王巍,孟宪生,包永睿,等.正交试验优选HPCE法测定人参中4种皂苷含量的方法研究[J].中药材,2011,34(12):1900-1903.
[46]胡斌,越皓,黄科科,等.纳升电喷雾萃取电离质谱快速测定人参皂苷[J].高等学校化学学报,2011,32(6):1289-1294.
[47]仇永跃,张金英,付迎.近红外光谱法测定生脉胶囊中人参皂苷Rg1和Re的含量[J].齐鲁药事,2011,30(10):585-587.
[48]汪静静,闫述模,杨滨.近红外光谱技术对人参药材人参皂苷含量测定及产地识别的研究[J].光谱学与光谱分析,2015,35(7):1885-1888.
[2]赵莉,郜玉钢,姬庆.人参化学成分的免疫作用及其机制的研究进展[J].中南药学,2015,13(7):741-745.
[3]林美妤,刘海宇,刘金平,等.人参皂苷的化学研究进展[J].人参研究,2011,23(4):43-46.
[4]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.地理标志产品吉林长白山人参[S].北京:中国标准出版社,2009.
[5]曲正义,赵景辉,刘宏群,等.比色法测定保鲜人参中人参总皂苷的含量[J].人参研究,2012,24(1):13-14.
[6]万玉华,戴洁,黄春静,等.紫外分光光度法测定参须中人参总皂苷的含量[J].中国中医药现代远程教育,2012,10(24):146-147.
[7]李青,刘思洁,石矛.食品中人参总皂苷的测定方法研究[J].人参研究,2014,26(3):17-19.
[8]王磊,孙德水,郑东梅.人参糖果中人参总皂苷含量的测定[J].人参研究,2013,25(4):28-30.
[9]庞威,赵宏峰,弥宏,等.紫外-可见分光光度法测定振源口服液(无糖型)中人参总皂苷的含量[J].中国医药指南,2014,29(12):59-60.
[10]张连龙,周华生,成恒嵩.测定参类保健食品中总皂苷的新方法[J].中国食品卫生杂志,2011,23(5):442-445.
[11]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.人参中多种人参皂苷含量的测定液相色谱-紫外检测法[S].北京:中国标准出版社,2008.
[12]赵亮,吕磊,纪松岗,等.高效液相色谱法快速测定人参中8种主要皂苷类成分的含量[J].第二军医大学学报,2008,29(12):1507-1510.
[13]刘志,阮长春,刘天志,等.HPLC法同时测定林下参、鲜人参、生晒参和红参中14种人参皂苷[J].中草药,2012,43(12):2431-2434.
[14]樊红秀,刘婷婷,刘鸿铖,等.超临界萃取人参皂苷及HPLC分析[J].食品科学,2013,34(20):121-126.
[15]袁斌,车金水,金燕,等.快速溶剂萃取-液相色谱法测定人参药材中人参皂苷的含量[J].药物分析杂志,2013,33(7):1267-1270.
[16]郜玉钢,郝建勋,臧埔,等.高效液相色谱法测定农田人参中9种人参皂苷单体含量[J].食品科学,2012,33(2):189-193.
[17]杨艳文,孟凡双,郜玉钢,等.高效液相色谱法同时测定人参制剂中20种人参皂苷方法的建立[J].食品科学,2016,37(22):131-135.
[18]张金秋,夏娟,孙光芝,等.RP-HPLC法测定人参花中11个人参皂苷含量[J].药物分析杂志,2015,35(9):1596-1600.
[19]曾露露,赵婷,李莹,等.高效液相色谱法同时测定黑参中4种稀有人参皂苷的含量[J].中国药学杂志,2017,52(12):1069-1072.
[20]SHI Y,SUN C J,ZHENG B,et al.Simultaneous determination of nine ginsenosides in functional foods by high performance liquid chromatography with diode array detector detection[J].Food Chem,2010,123(4):1322-1327.
[21]KIM S N,HA Y W,SHIN H,et al.Simultaneous quantification of 14ginsenosides in Panax ginseng C.A.Meyer(Korean red ginseng)by HPLC-ELSD and its application to quality control[J].J Pharm Biomed Anal,2007,45(1):164-170.
[22]SUN B S,GU L J,FANG Z M,et al.Simultaneous quantification of 19ginsenosides in black ginseng developed from Panax ginseng by HPLC–ELSD[J].J Pharm Biomed Anal,2009,50(1):15-22.
[23]LEE S I,KWON H J,LEE Y M,et al.Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD[J].J Pharm Biomed Anal,2012(60):80-85.
[24]崔勇,李青,刘思洁,等.固相萃取-超高效液相色谱法同时测定人参中11种人参皂苷的含量[J].中国卫生检验杂志,2012,22(3):475-477.
[25]PARK H W,IN G,HAN S T,et al.Simultaneous determination of 30ginsenosides in Panax ginseng preparations using ultra performance liquid chromatography[J].J Ginseng Res,2013,37(4):457-467.
[26]逄世峰,孙成贺,李亚丽,等.MCI前处理-UPLC法测定西洋参茎叶中7种人参皂苷含量[J].特产研究,2014(1):49-51.
[27]张晓旭,马雪涛,胡蒙,等.超高效液相色谱法检测6种人参皂苷含量[J].中国食品学报,2015,15(5):241-246.
[28]张玉婷,冯克然,曹进,等.UPLC法评价多种人参提取物中人参皂苷的含量[J].食品科学,2013,34(24):102-105.
[29]李薇,吉利娜,宋新波.UPLC法测定人参流动浸膏中8种人参皂苷类成分[J].天津中医药,2014,31(10):624-626.
[30]蔡艳,宋剑,王贵金,等.UPLC法测定人参总皂苷提取物中7种人参皂苷的含量[J].西北药学杂志,2016,31(5):441-444.
[31]刘婷,徐虹.超高效液相色谱法快速测定参麦注射液中6种皂苷类成分含量[J].医药导报,2016,35(11):1259-1261.
[32]孔宏伟,王梅,Rob H J,等.HPLC/LTQ-FTMS用于白参中的丙二酰基人参皂苷的研究[J].世界科学技术-中医药现代化,2009,11(1):190-194.
[33]孙海,张亚玉,王英平,等.利用HPLC/MS对野山参和林下参皂苷的分析[J].特产研究,2011,33(3):52-55.
[34]马浩然,程璐,王富乾,等.HPLC-MS/MS法测定10批参须中人参皂苷Rg1、Re和Rb1的含量[J].中南药学,2017,15(6):807-810.
[35]STAVRIANIDI A,STEKOLSHCHIKOVA E,POROTOVA A,et al.Combination of HPLC–MS and QAMS as a new analytical approach fordetermination of saponins in ginseng containing products[J].J Pharm Biomed Anal,2017(132):87-92.
[36]陈洪岩,姚晨旭,张小龙.高效液相色谱四级杆飞行时间串联质谱法测定启脾丸中7种人参皂苷的含量[J].中国药师,2017,20(3):460-462.
[37]Li L,Luo G A,Liang Q L,et al.Rapid qualitative and quantitative analyses of Asian ginseng in adulterated American ginseng preparations by UPLC/Q-TOF-MS[J].J Pharm Biomed Anal,2010,52(1):66-72.
[38]Zhang H M,Li S L,Zhang H,et al.Holistic quality evaluation of commercial white and red ginseng using a UPLC-QTOF-MS/MS-based metabolomics approach[J].J Pharm Biomed Anal,2012,62(62):258-273.
[39]张晓旭,王洪平,杨阳,等.超高效液相色谱-串联四极杆飞行时间质谱法快速鉴别生晒参中的皂苷类成分[J].中国医药导报,2015,12(9):130-136.
[40]WANG H P,ZHANG Y B,YANG X W,et al.Rapid characterization of ginsenosides in the roots and rhizomes of Panax ginseng by UPLC-DADQTOF-MS/MS and simultaneous determination of 19 ginsenosides by HPLC-ESI-MS[J].J Ginseng Res,2016,40(4):382-394.
[41]CHEN Y J,ZHAO Z Z,CHEN H B,et al.Determination of ginsenosides in Asian and American ginsengs by liquid chromatography-quadrupole/time-of-flight MS:assessing variations based on morphological characteristics[J].J Ginseng Res,2017,41(1):10-22.
[42]钟薇,戴雨霖,李晓宇,等.超高效液相色谱-四极杆-静电场轨道阱质谱法分析不同炮制红参的化学成分差异[J].质谱学报,2015,36(6):529-534.
[43]张勇,黄鑫,李帅坪,等.UPLC Orbitrap HRMS法分析西洋参蒸参弃液浓缩物中皂苷类成分[J].质谱学报,2017,38(1):52-59.
[44]王淑美,王瑞,梁生旺,等.胶束电动毛细管色谱法分离分析人参中人参皂苷Rg1和人参皂苷Re[J].中药材,2009,32(7):1069-1071.
[45]王巍,孟宪生,包永睿,等.正交试验优选HPCE法测定人参中4种皂苷含量的方法研究[J].中药材,2011,34(12):1900-1903.
[46]胡斌,越皓,黄科科,等.纳升电喷雾萃取电离质谱快速测定人参皂苷[J].高等学校化学学报,2011,32(6):1289-1294.
[47]仇永跃,张金英,付迎.近红外光谱法测定生脉胶囊中人参皂苷Rg1和Re的含量[J].齐鲁药事,2011,30(10):585-587.
[48]汪静静,闫述模,杨滨.近红外光谱技术对人参药材人参皂苷含量测定及产地识别的研究[J].光谱学与光谱分析,2015,35(7):1885-1888.