HPLC波长切换法同时测定参麦颗粒中6种成分的含量
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摘要
目的建立HPLC波长切换法同时测定参麦颗粒中的尿囊素、原儿茶醛、儿茶素、表儿茶素、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B的含量。方法采用Agilent C_(18)(4. 6 mm×250 mm,5μm);以乙腈-0. 5%冰醋酸溶液为流动相,梯度洗脱;柱温30℃。结果尿囊素、原儿茶醛、儿茶素、表儿茶素、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B的线性范围分别为3. 87～96. 75μg/ml (r=0. 999 9)、1. 29～32. 25μg/ml(r=0. 999 3)、0. 73～18. 25μg/ml (r=0. 999 2)、1. 56～39. 00μg/ml (r=0. 999 8)、1. 19～29. 75μg/ml (r=0. 999 6)和0. 86～21. 50μg/ml (r=0. 999 5),平均加样回收率分别为99. 46%、98. 28%、96. 97%、98. 55%、97. 99%、97. 26%,RSD分别为1. 10%、0. 96%、1. 43%、1. 29%、0. 77%、1. 36%。结论所建立的HPLC波长切换法可同时测定参麦颗粒中尿囊素、原儿茶醛、儿茶素、表儿茶素、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B的含量,可以为参麦颗粒的质量控制提供依据。
Objective To determine the content of allantoin,protocatechuic aldehyde, (+)-catechin,L-epicatechin,methylophiopogonanone A and methylophiopogonanone B in Shenmai granules simultaneously by HPLC wavelength switching method. Methods The Agilent C_(18) column (4. 6 mm × 250 mm,5 μm) was adopted,the column temperature was set at 30 ℃,and the mobile phase consisted of acetonitrile-0. 5% glacial acetic acid solution with gradient elution. Results Allantoin,protocatechuic aldehyde, (+)-catechin,L-epicatechin,methylophiopogonanone A and methylophiopogonanone B showed a good linear relationship in the range of 3. 87 ～ 96. 75 μg/ml (r = 0. 999 9),1. 29 ～ 32. 25 μg/ml (r = 0. 999 3),0. 73 ～ 18. 25 μg/ml (r = 0. 999 2),1. 56 ～ 39. 00 μg/ml (r = 0. 999 8),1. 19 ～29. 75 μg/ml (r = 0. 999 6),and 0. 86 ～ 21. 50 μg/ml (r = 0. 999 5),respectively. The average recoveries were99. 46%,98. 28%,96. 97%,98. 55%,97. 99% and 97. 26%,respectively; the RSD was 1. 10%,0. 96%,1. 43%,1. 29%,0. 77% and 1. 36%,respectively. Conclusion The established HPLC wavelength switching method for simultaneous determination of the content of allantoin,protocatechuic aldehyde, (+)-catechin,L-epicatechin,methylophiopogonanone A and methylophiopogonanone B in Shenmai granules can be applied to the quality control of Shenmai granules.
Objective To determine the content of allantoin,protocatechuic aldehyde, (+)-catechin,L-epicatechin,methylophiopogonanone A and methylophiopogonanone B in Shenmai granules simultaneously by HPLC wavelength switching method. Methods The Agilent C_(18) column (4. 6 mm × 250 mm,5 μm) was adopted,the column temperature was set at 30 ℃,and the mobile phase consisted of acetonitrile-0. 5% glacial acetic acid solution with gradient elution. Results Allantoin,protocatechuic aldehyde, (+)-catechin,L-epicatechin,methylophiopogonanone A and methylophiopogonanone B showed a good linear relationship in the range of 3. 87 ～ 96. 75 μg/ml (r = 0. 999 9),1. 29 ～ 32. 25 μg/ml (r = 0. 999 3),0. 73 ～ 18. 25 μg/ml (r = 0. 999 2),1. 56 ～ 39. 00 μg/ml (r = 0. 999 8),1. 19 ～29. 75 μg/ml (r = 0. 999 6),and 0. 86 ～ 21. 50 μg/ml (r = 0. 999 5),respectively. The average recoveries were99. 46%,98. 28%,96. 97%,98. 55%,97. 99% and 97. 26%,respectively; the RSD was 1. 10%,0. 96%,1. 43%,1. 29%,0. 77% and 1. 36%,respectively. Conclusion The established HPLC wavelength switching method for simultaneous determination of the content of allantoin,protocatechuic aldehyde, (+)-catechin,L-epicatechin,methylophiopogonanone A and methylophiopogonanone B in Shenmai granules can be applied to the quality control of Shenmai granules.
引文
[1]中华人民共和国卫生部药典委员会.卫生部颁药品标准(中药成方制剂第十三册)[M].北京:人民卫生出版社,1997:119.
[2]乔宇,廖李,汪兰,等.不同山药品种尿囊素含量的测定[J].湖北农业科学,2014,53(22):5528-5530.
[3]明鹤,杨太新,杜艳华.HPLC分析不同施肥山药中尿囊素及薯蓣皂苷元含量[J].中药材,2014,37(4):558-560.
[4]谭亮,董琦,肖远灿,等.RP-HPLC法同时测定枸杞子中5个酚酸类成分的含量[J].药物分析杂志,2013,33(3):376-381,394.
[5]冯怡,李媛,付荣杰,等.儿茶及儿茶提取物的质量标准研究[J].中成药,2004,26(4):325-328.
[6]吴发明,杨瑞山,张思荻,等.高效液相色谱法同时测定麦冬中3个黄酮成分的含量[J].中国药学杂志,2016,51(8):655-658.
[7]吴发明,张思荻,曾俊,等.HPLC-ELSD法测定不同产地麦冬中4种代表性成分的含量[J].药物分析杂志,2016,36(8):1370-1376.
[8]文庆,聂平,丁野,等.HILIC-HPLC法测定山药中尿囊素的含量[J].中南药学,2014,12(2):169-171.
[2]乔宇,廖李,汪兰,等.不同山药品种尿囊素含量的测定[J].湖北农业科学,2014,53(22):5528-5530.
[3]明鹤,杨太新,杜艳华.HPLC分析不同施肥山药中尿囊素及薯蓣皂苷元含量[J].中药材,2014,37(4):558-560.
[4]谭亮,董琦,肖远灿,等.RP-HPLC法同时测定枸杞子中5个酚酸类成分的含量[J].药物分析杂志,2013,33(3):376-381,394.
[5]冯怡,李媛,付荣杰,等.儿茶及儿茶提取物的质量标准研究[J].中成药,2004,26(4):325-328.
[6]吴发明,杨瑞山,张思荻,等.高效液相色谱法同时测定麦冬中3个黄酮成分的含量[J].中国药学杂志,2016,51(8):655-658.
[7]吴发明,张思荻,曾俊,等.HPLC-ELSD法测定不同产地麦冬中4种代表性成分的含量[J].药物分析杂志,2016,36(8):1370-1376.
[8]文庆,聂平,丁野,等.HILIC-HPLC法测定山药中尿囊素的含量[J].中南药学,2014,12(2):169-171.