ERK通路蛋白、ANXA2及FOXJ1蛋白表达与胃癌患者临床分期及预后的相关性研究
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摘要
目的对细胞外调节蛋白激酶(ERK)通路蛋白、膜联蛋白A2(ANXA2)及叉头框蛋白J1(FOXJ1)蛋白表达与胃癌患者临床分期及预后的相关性进行研究,为临床诊断和治疗提供参考。方法对2011年3月至2015年12月期间诊治的180例胃癌患者进行研究,其中0期38例,Ⅰ期者36例,Ⅱ期30例,Ⅲ期37例,Ⅳ期39例。同期健康体检者30例为对照组。分析各研究对象血清ANXA2及FOXJ1蛋白表达与胃癌患者临床分期及预后的相关性。结果与对照组比较,胃癌组Ras、Raf、MEK、ERK1/2、ANXA2和ANXA4、FOXJ1及TAP1蛋白均明显升高,且随着胃癌临床分期的增加,上述蛋白的表达明显增强(P<0.05);ANXA2及FOXJ1蛋白与胃癌患者临床预后有密切的相关性,且随着ANXA2蛋白表达的增强患者的预后变差。结论 ERK信号通路蛋白、ANXA2及FOXJ1蛋白表达与胃癌患者临床分期及预后有明显的相关性。
Objective The present research aimed to explore the relationship of serum ERK signaling pathway proteins and ANXA2,FOXJ1 with the clinical stage and prognosis of gastric cancer patients. Methods 180 cases of gastric cancer patients in our department from Mar2011 to Dec 2015 were enrolled in this part. 38 cases in stage 0,36 cases in stage Ⅰ,30 cases in stage Ⅱ,37 cases in stage Ⅲ and 39 cases in stage Ⅳ. The relationship of serum ERK signaling pathway proteins and ANXA2,FOXJ1 with the clinical stage and prognosis in gastric cancer patients were analyzed. Results Compared with the control group,Ras,Raf,MEK,ERK1 /2,ANXA2,ANXA4,FOXJ1 and TAP1 protein were significantly increased in gastric cancer group. And the expression of these proteins was enhanced with the clinical stage of gastric cancer.ANXA2 and FOXJ1 protein were closely related with the clinical prognosis,and with the enhancement of ANXA2 protein expression,the prognosis was poor. Conclusion ERK signaling pathway proteins and the expression of ANXA2,FOXJ1 have a close relationship with the clinical stage and prognosis of gastric cancer patients.
Objective The present research aimed to explore the relationship of serum ERK signaling pathway proteins and ANXA2,FOXJ1 with the clinical stage and prognosis of gastric cancer patients. Methods 180 cases of gastric cancer patients in our department from Mar2011 to Dec 2015 were enrolled in this part. 38 cases in stage 0,36 cases in stage Ⅰ,30 cases in stage Ⅱ,37 cases in stage Ⅲ and 39 cases in stage Ⅳ. The relationship of serum ERK signaling pathway proteins and ANXA2,FOXJ1 with the clinical stage and prognosis in gastric cancer patients were analyzed. Results Compared with the control group,Ras,Raf,MEK,ERK1 /2,ANXA2,ANXA4,FOXJ1 and TAP1 protein were significantly increased in gastric cancer group. And the expression of these proteins was enhanced with the clinical stage of gastric cancer.ANXA2 and FOXJ1 protein were closely related with the clinical prognosis,and with the enhancement of ANXA2 protein expression,the prognosis was poor. Conclusion ERK signaling pathway proteins and the expression of ANXA2,FOXJ1 have a close relationship with the clinical stage and prognosis of gastric cancer patients.
引文
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[16]奉艳,肖丽,何慧敏,等.ANXA2对Ca CO2细胞凋亡的影响[J].陕西师范大学学报(自然科学版),2015,56(1):75-79.
[17]郭继东,丁照义,王策.膜联蛋白A2在脑胶质瘤组织中的表达及其与细胞凋亡的相关性[J].中国老年学杂志,2013,33(2):417-418.
[18]朴素宙,梁丽梅,王斌.膜联蛋白A2在宫颈癌组织中的表达及其临床意义[J].中国妇幼保健,2013,28(14):2323-2324.
[19]刘秀鹏,赵素芳,胡晶晶,等.FOXJ1在胃癌组织中的表达及其与临床病理特征和预后的相关性[J].海南医学,2016,44(2):192-194.
[20]刘向强,张志勇,孙力.MicroRNA-499-5p通过靶向FOX04和PDCD4促进大肠癌细胞侵袭和肿瘤转移[J].医学争鸣,2012,33(4):3-4.
[2]黄迪,李飞薄,玉琨,等.GLO1在胃癌组织中的表达研究[J].临床和实验医学杂志,2015,14(13):1051-1054.
[3]沈洁,马娅梅.COX-2及c-met蛋白在疣状胃炎与胃癌中的表达及其意义[J].广州医科大学学报,2014,24(5):102-104.
[4]丁滔,郑江花,洪斌,等.下调Annexin A2的表达在促前列腺癌进展中的作用[J].肿瘤防治研究,2014,41(7):745-750.
[5]邓宇,王雅琪,李云翠,等.H4K16ac调控Ras诱导的乳腺癌细胞迁移、增殖能力机理研究[J].基因组学与应用生物学,2015,40(11):2306-2313.
[6]李继强,陈谦学.缺氧诱导因子1α相关信号通路与肿瘤关系的研究进展[J].医学综述,2015,21(24):4460-4464.
[7]韩晶,吴文英.桂枝茯苓丸对子宫内膜异位症患者血清MAPK、ERK和VEGF水平影响研究[J].中国生化药物杂志,2015,20(12):125-127.
[8]阿茹娜.根蛋白通过Rac1-ERK途径提高MMP-7合成,从而增强结肠癌细胞的侵袭性[J].中国普外基础与临床杂志,2015,22(1):75-76.
[9]陆国玉,吴晓飞,周蕾,等.ERK1和E-cadherin在胃腺癌中的表达及其临床病理意义[J].中国组织化学与细胞化学杂志,2015,5(1):72-77.
[10]林永利,赵海鹰,刘金钢,等.ERK1/2和p-ERK1/2在胆管癌组织中的表达及临床意义[J].中国医药导报,2015,36(7):19-23.
[11]车佳.水飞蓟宾通过降低MEK/ERK途径依赖的MMP-9蛋白表达抑制人乳腺癌细胞生长和侵袭转移机制的实验研究[J].浙江中西医结合杂志,2015,25(4):337-340.
[12]肖高春,童仕伦,郑勇斌,等.PI3K/AKT及MEK/ERK信号通路在肿瘤血管内皮细胞迁移中的作用[J].重庆医学,2015,44(11):1452-1456.
[13]燕翔,焦顺昌.肿瘤浸润淋巴细胞与非小细胞肺癌预后关系的Meta分析[J].中国医学科学院学报,2015,37(4):406-414.
[14]侯俊杰,周颖,谭岩,等.膜联蛋白A2与肿瘤进展的相关性[J].中国免疫学杂志,2015,31(11):1573-1577.
[15]徐凯,冒晓蓓,褚晓源.叉头框蛋白M1在肿瘤信号转导中的作用[J].医学研究生学报,2014,27(11):1226-1228.
[16]奉艳,肖丽,何慧敏,等.ANXA2对Ca CO2细胞凋亡的影响[J].陕西师范大学学报(自然科学版),2015,56(1):75-79.
[17]郭继东,丁照义,王策.膜联蛋白A2在脑胶质瘤组织中的表达及其与细胞凋亡的相关性[J].中国老年学杂志,2013,33(2):417-418.
[18]朴素宙,梁丽梅,王斌.膜联蛋白A2在宫颈癌组织中的表达及其临床意义[J].中国妇幼保健,2013,28(14):2323-2324.
[19]刘秀鹏,赵素芳,胡晶晶,等.FOXJ1在胃癌组织中的表达及其与临床病理特征和预后的相关性[J].海南医学,2016,44(2):192-194.
[20]刘向强,张志勇,孙力.MicroRNA-499-5p通过靶向FOX04和PDCD4促进大肠癌细胞侵袭和肿瘤转移[J].医学争鸣,2012,33(4):3-4.