重楼水提取液对大鼠离体胃肠运动的影响及双向调节作用观察
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摘要
目的观察重楼水提取液对大鼠离体胃肠运动的影响及其双向调节作用。方法采用常规离体小肠平滑肌灌流法,观察重楼水提取液对正常、抑制(阿托品诱导)、亢进(乙酰胆碱诱导)的离体胃肠平滑肌收缩幅度和频率的影响。结果低、高剂量重楼水提液使大鼠离体胃平滑肌收缩幅度减小和频率变慢(P均<0. 01),使大鼠离体小肠平滑肌收缩幅度增大(P <0. 05或<0. 01);低剂量重楼水提液使大鼠离体小肠平滑肌收缩频率减慢(P均<0. 01),但高剂量重楼水提液作用后收缩频率无明显变化(P均> 0. 05)。低、高剂量重楼水提液可使阿托品诱导的舒张胃肠平滑肌收缩幅度增大且频率加快(P <0. 05或<0. 01),可使乙酰胆碱诱导的兴奋胃肠平滑肌收缩幅度减小(P <0. 05或<0. 01)。结论重楼水提液可抑制大鼠离体胃平滑肌收缩、兴奋大鼠离体小肠平滑肌收缩,并且对阿托品、乙酰胆碱诱导的离体胃肠运动具有双向调节作用。
Objective To investigate the effects of Paris polyphylla water extracts on rats' isolated gastrointestinal motility and bidirectional regulation. Methods Routine smooth muscle reperfusion of isolated small intestine was applied to observe the effects of Paris polyphylla water extracts on the amplitude and frequency of contraction of isolated gastrointestinal smooth muscles,which were normal,inhibited( with induction of atropine) or hyperfunctional( with induction of acetylcholine). Results Low-dose and high-dose Paris polyphylla water extracts reduced the amplitude and frequency of contraction of isolated gastric smooth muscle in rats( both P < 0. 01),and increased the amplitude of contraction of isolated intestinal smooth muscle in rats( P < 0. 05 or P < 0. 01). The contraction frequency of isolated intestinal smooth muscle in rats was slowed down by low-dose Paris polyphylla water extracts( P < 0. 01),but there was no significant change in contraction frequency after high dose( P > 0. 05). Low and high doses of Paris polyphylla water extract increased and accelerated the contraction amplitude of diastolic gastrointestinal smooth muscle induced by atropine( P < 0. 05 or P < 0. 01),and decreased the contraction amplitude of gastrointestinal smooth muscle induced by acetylcholine( P < 0. 05 or P < 0. 01).Conclusion Paris polyphylla water extracts can inhibit the contraction of isolated gastric smooth muscle and excited isolated small intestinal smooth muscle of rats,and it has a bidirectional regulation effect on the isolated gastrointestinal movement induced by atropine and acetylcholine.
Objective To investigate the effects of Paris polyphylla water extracts on rats' isolated gastrointestinal motility and bidirectional regulation. Methods Routine smooth muscle reperfusion of isolated small intestine was applied to observe the effects of Paris polyphylla water extracts on the amplitude and frequency of contraction of isolated gastrointestinal smooth muscles,which were normal,inhibited( with induction of atropine) or hyperfunctional( with induction of acetylcholine). Results Low-dose and high-dose Paris polyphylla water extracts reduced the amplitude and frequency of contraction of isolated gastric smooth muscle in rats( both P < 0. 01),and increased the amplitude of contraction of isolated intestinal smooth muscle in rats( P < 0. 05 or P < 0. 01). The contraction frequency of isolated intestinal smooth muscle in rats was slowed down by low-dose Paris polyphylla water extracts( P < 0. 01),but there was no significant change in contraction frequency after high dose( P > 0. 05). Low and high doses of Paris polyphylla water extract increased and accelerated the contraction amplitude of diastolic gastrointestinal smooth muscle induced by atropine( P < 0. 05 or P < 0. 01),and decreased the contraction amplitude of gastrointestinal smooth muscle induced by acetylcholine( P < 0. 05 or P < 0. 01).Conclusion Paris polyphylla water extracts can inhibit the contraction of isolated gastric smooth muscle and excited isolated small intestinal smooth muscle of rats,and it has a bidirectional regulation effect on the isolated gastrointestinal movement induced by atropine and acetylcholine.
引文
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[15]向德标,阳漾,刘小娟,等.地锦草醇提物对兔离体小肠平滑肌作用及机制研究[J].中国医院药学杂志,2015,35(15):1371-1374.
[16]张佳,邵正威,高雅,等.槲皮素对兔离体肠平滑肌的舒张作用及其机制[J].中国应用生理学杂志,2013,29(2):162-165.
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[18]苏佳,刘泽源,赵振虎,等. PLA2/AA信号途径与重楼皂苷诱导大鼠子宫平滑肌收缩活动的关系研究[J].军事医学科学院院刊,2008,32(3):264.
[19] Corea G,Fattorusso E,Lanzotti V. Antispasmodic saponins from bulbs of red onion,Allium cepa L. var,Tropea[J]. J Agric FoodChem,2005,53(4):935-940.
[2]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:243-244.
[3]卢伟,杨光义,叶方,等.正交试验设计优选重楼总皂苷提取工艺[J].中国药师,2015,18(9):1484-1487.
[4]杨远贵,张霁,张金渝,等.重楼属植物化学成分及药理活性研究进展[J].中草药,2016,47(18):3301-3323.
[5]刘湘丹,王朝晖,徐玉琴,等.中药材重楼研究进展[J].中医药导报,2015,21(15):90-93.
[6]华栋.宽叶重楼的化学成分研究[D].西安:第四军医大学,2015:1-94.
[7]杨光义,胡培,叶方.重楼资源分布与可持续利用研究进展[J].中国药师,2016,19(1):159-162.
[8]Liu ZX,Liu ZX,Tian QJ. Analysis of chemical components of volatileoil from Paris polyphla and their antibacterial activities[J].Zhong Yao Cai,2014,37(4):612-616.
[9]熊伟,修光辉,周霞,等.中药重楼抗炎活性成分的临床应用研究进展[J].云南中医中药杂志,2016,37(9):86-88.
[10]马林喜,王俊丽,费良丹,等.武陵山地区特色药用植物七叶一枝花的研究与应用[J].中央民族大学学报(自然科学版),2014,23(3):75-79.
[11]黄彦峰,何显教,晋玲,等.重楼水提液对小鼠胃肠运动功能的影响[J].医药导报,2014,33(4):442-445.
[12]王慧,张海娟,李志东.忍冬藤提取物对兔离体肠平滑肌的舒张作用及其机制[J].中国农业科学,2017,50(2):372-379.
[13]Ori K,Mimaki Y,Mito K,et al. Jatropham derivatives and steroidalsaponins from the bulbs of Lilium hansonii[J]. Phytochemistry,1992,31(8):2767-2775.
[14]边晶,李洲.双向调节作用理论与临床研究进展[J].中国城乡企业卫生,2016(9):49-51.
[15]向德标,阳漾,刘小娟,等.地锦草醇提物对兔离体小肠平滑肌作用及机制研究[J].中国医院药学杂志,2015,35(15):1371-1374.
[16]张佳,邵正威,高雅,等.槲皮素对兔离体肠平滑肌的舒张作用及其机制[J].中国应用生理学杂志,2013,29(2):162-165.
[17]刘星,余江丽,刘敏,等.近10年甾体皂苷的生物活性研究进展[J].中国中药杂志,2015,40(13):2518-2523.
[18]苏佳,刘泽源,赵振虎,等. PLA2/AA信号途径与重楼皂苷诱导大鼠子宫平滑肌收缩活动的关系研究[J].军事医学科学院院刊,2008,32(3):264.
[19] Corea G,Fattorusso E,Lanzotti V. Antispasmodic saponins from bulbs of red onion,Allium cepa L. var,Tropea[J]. J Agric FoodChem,2005,53(4):935-940.