摘要
目的考察厚朴远志配伍对大鼠尿液代谢物的影响,探索其特征生物标志物并分析相关代谢通路,为关联探索厚朴缓解远志胃肠动力障碍的可能机制提供参考。方法 SD♂大鼠40只,随机分为对照组、厚朴3. 50 g·kg~(-1)组、远志1. 75 g·kg~(-1)组、配伍3. 5+1. 75 g·kg~(-1)组,连续灌胃3 d,收集大鼠末次给药24 h内的尿液,采用UPLC-Q-TOF-MS检测各组尿液的代谢物,借助Progenesis QI V2. 0、SIMCA-P14. 0、SPSS 20. 0、Metabo Analyst 3. 0等软件,分析各组尿液代谢标志物及其代谢通路。结果对照组与给药组的代谢物能够明显区分,并筛选出2-氨基苯甲酸、D-葡萄糖醛酸-6,3-内酯、3-甲氧基-4-羟基苯乙醛等16个特征代谢标志物,主要涉及酪氨酸代谢、色氨酸代谢,以及苯丙氨酸、酪氨酸、色氨酸生物合成等通路。结论厚朴可能通过调控酪氨酸代谢、色氨酸代谢、初级胆汁酸生物合成和维生素B_6代谢等代谢通路,缓解远志所致胃肠动力障碍。
Aim To study the effect of compatibility of Magnolia officinalis and Polygala tenuifolia on urine metabolites in rats,to explore its characteristic biomarkers and analyze the related metabolic pathways,and to provide references for exploring the possible mechanisms of Magnolia officinalis relieving gastrointestinal motility disorders induced by Polygala tenuifolia.Methods Forty male Sprague-Dawley rats were randomly divided into control group,Polygala tenuifolia1. 75 g · kg~(-1) group,Magnolia officinalis 3. 50 g ·kg~(-1) group, and the compatibility group( 3. 50 +1. 75) g · kg~(-1),continuously orally administered for three days. Urine was collected within 24 hours after the last administration and detected by UPLC-Q-TOFMS in each group. The characteristic biomarkers and corresponding metabolic pathways were analyzed or screened by Progenesis QI V2. 0, SIMCA-P 14. 0,SPSS 20. 0,MetaboAnalyst 3. 0 and other softwares.Results The metabolites in control group and drugadministered group could be clearly distinguished,and a total of 16 characteristic metabolites,2-Aminobenzoic acid, D-Glucurono-6, 3-lactone and Homovanillin,were screened out. The main related pathways included Tyrosine metabolism,tryptophan metabolism,vitamin B_6 metabolism and primary bile acid biosynthesis. Conclusions The mechanism of Magnolia officinalis relieving gastrointestinal motility disorders induced by Polygala tenuifolia might be related with Tyrosine metabolism,tryptophan metabolism,primary bile acid biosynthesis and vitamin B_6 metabolism.
引文
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