基于溶血率的金银花与山银花质量差异研究
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摘要
通过研究金银花药材与山银花药材的体外溶血作用,对二者的内在质量差异进行研究.分析3批金银花药材与3批山银花药材体外溶血作用,计算溶血率,运用不同的统计分析方法分析二者是否存在差异.结果显示,运用SPSS相似度软件对二者进行分析时,金银花样品与3批山银花样品相似度分别为73.3%、60.0%、27.6%;金银花样品在溶血率—质量浓度曲线上出现溶血率最大值时的质量浓度较山银花大,且溶血率最大值小于山银花;采用"Smirnov"法分析,2批山银花样品与金银花样品具有显著差异.实验表明,金银花和山银花在体外溶血方面存在差异,说明金银花药材与山银花药材内在质量存在差异.
In this paper,by studying the in vitro hemolysis of two groups of Flos Lonicerae and Chinese honeysuckle,the internal quality differences between these two groups were studied.The in vitro hemolysis of three batches of Flos Lonicerae and that of three batches of Radix Lonicerae were analyzed,and the hemolysis rate was calculated.Different statistic analysis methods were used to analyze whether differences existed in these two groups.The results showed that when using SPSS similarity software to analyze them,the similarity between honeysuckle samples and three batches of Flos Lonicerae samples was 73.3%,60.6% and 27.6%.The curve of the hemolysis and mass concentration showed that the maximum hemolysis rate of honeysuckle sample was higher than that of honeysuckle sample,and the maximum hemolysis rate of honeysuckle sample was lower than that of honeysuckle sample.The analysis done by Smirnov method showed that there existed noticeable differences between these two groups of samples.It is concluded that there are differences in vitro hemolysis between honeysuckle and Flos Lonicerae,and the intrinsic quality of honeysuckle is also different from that of Flos Lonicerae.
In this paper,by studying the in vitro hemolysis of two groups of Flos Lonicerae and Chinese honeysuckle,the internal quality differences between these two groups were studied.The in vitro hemolysis of three batches of Flos Lonicerae and that of three batches of Radix Lonicerae were analyzed,and the hemolysis rate was calculated.Different statistic analysis methods were used to analyze whether differences existed in these two groups.The results showed that when using SPSS similarity software to analyze them,the similarity between honeysuckle samples and three batches of Flos Lonicerae samples was 73.3%,60.6% and 27.6%.The curve of the hemolysis and mass concentration showed that the maximum hemolysis rate of honeysuckle sample was higher than that of honeysuckle sample,and the maximum hemolysis rate of honeysuckle sample was lower than that of honeysuckle sample.The analysis done by Smirnov method showed that there existed noticeable differences between these two groups of samples.It is concluded that there are differences in vitro hemolysis between honeysuckle and Flos Lonicerae,and the intrinsic quality of honeysuckle is also different from that of Flos Lonicerae.
引文
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[2]康帅,张继,王亚丹,等.金银花与山银花的生药学鉴别研究[J].药物分析杂志,2014,34(11):1913-1921.
[3]刘玉琴.山银花中皂苷含量测定及指纹图谱研究[D].长沙:湖南中医药大学,2014.
[4]杨倩茹,赵媛媛,郝江波,等.金银花与山银花化学成分及其差异的研究进展[J].中草药,2014,45(8):1150-1156.
[5]黄娜,高慧敏,陈两绵,等.金银花与山银花的体外溶血作用分析[J].中国实验方剂学杂志,2017,23(12):9-12.
[6]沈放,杨黎江,彭永芳,等.重楼皂苷类化合物溶血作用研究[J].时珍国医国药,2010,21(9):2280-2281.
[7]张国松,封传华,罗晓健,等.柴胡总皂苷提取物体外溶血作用[J].中国实验方剂学杂志,2011,17(20):166-169.
[8]刘墨样,于红艳,黄樱,等.桔梗总皂苷与其总次皂苷溶血效应的比较研究[J].扬州大学学报(农业与生命科学版),2006,27(4):26-28.
[9]程大任,付锐,窦德强,等.人参皂苷溶血及抗溶血作用研究[J].中国现代中药,2007,9(4):19-23.
[10]杨广,项峥,康廷国,等.人参皂苷溶血测定的实验条件研究[J].辽宁中医药大学学报,2008,10(6):190-191.
[11]刘涛,王鸿,李青苗,等.不同采收期金银花的产地加工方法考察[J].中国实验方剂学杂志,2016,22(10):28-31.
[12]杨光.人参皂苷溶血规律研究[D].沈阳:辽宁中医药大学,2008.