臭氧水灌洗阴道对正常阴道微生态及乳杆菌影响的研究
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摘要
目的探讨臭氧水灌洗阴道对正常阴道微生态及阴道乳杆菌的影响。方法选取2016年4月至10月于北京清华长庚医院女性职工中招募阴道微生态检查结果正常的志愿者30例,非月经期每天接受臭氧水灌洗阴道5min,连续3 d。分别在初次阴道臭氧水灌洗前、末次灌洗后的第1周、第2周、第3周留取阴道分泌物,进行阴道微生态检测及阴道微生物16S rRNA高通量测序后对菌群多样性和丰度进行分析。结果微生态检查结果正常的女性接受臭氧水灌洗阴道前后,其阴道微生态、阴道内菌群多样性及乳杆菌无显著差异(cor.p绝对值<0.2)。结论臭氧水灌洗阴道不破坏正常阴道微生态环境,对乳杆菌无显著影响。
Objective To explore the changes of normal vaginal micro-ecological environment after vaginal lavage with ozonated water and its effect on lactobacillus.Methods From April to October 2016,30 volunteers with normal vaginal microecological examination results were recruited from female workers in Beijing Tsinghua Changgung Hospital and selected to receive vaginal lavage with ozonated water,during non-menstrual periods 5 minutes per day,for 3 consecutive days.The vaginal secretions were taken before the first vaginal ozone lavage,the first week,the second week and the third week after the last lavage,and the microecological examination and the difference in the diversity and abundance of the vaginal microbial community were analyzed after 16 S rRNA high-through put sequencing.Results Before and after healthy women received ozone water for vaginal lavage,there were no significant differences in the microflora evaluation of vaginal secretions,vaginal microbial community diversity or lactobacillus(|cor. p| <0.2).Conclusion Ozonated water for vaginal lavage does not destroy the normal vaginal microecology and has no significant effect on lactobacilli.
Objective To explore the changes of normal vaginal micro-ecological environment after vaginal lavage with ozonated water and its effect on lactobacillus.Methods From April to October 2016,30 volunteers with normal vaginal microecological examination results were recruited from female workers in Beijing Tsinghua Changgung Hospital and selected to receive vaginal lavage with ozonated water,during non-menstrual periods 5 minutes per day,for 3 consecutive days.The vaginal secretions were taken before the first vaginal ozone lavage,the first week,the second week and the third week after the last lavage,and the microecological examination and the difference in the diversity and abundance of the vaginal microbial community were analyzed after 16 S rRNA high-through put sequencing.Results Before and after healthy women received ozone water for vaginal lavage,there were no significant differences in the microflora evaluation of vaginal secretions,vaginal microbial community diversity or lactobacillus(|cor. p| <0.2).Conclusion Ozonated water for vaginal lavage does not destroy the normal vaginal microecology and has no significant effect on lactobacilli.
引文
[1]廖秦平,陈锐.大数据在妇产科微生态领域中的应用现状及展望[J].中国实用妇科与产科杂志,2018,34(1):52-55.
[2]Antonio MA,Hawes SE,Hillier SL.The identification of vaginal lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species[J].J Infect Dis,1999,180(6):1950-1956.
[3]Ravel J,Gajer P,Abdo Z,et al.Vaginal microbiome of reproductiveage women[J].Proc Natl Acad Sci U S A,2011,108(Suppl1):4680-4687.
[4]廖秦平,牛小溪.人体微生态研究现状与展望[J].中国实用妇科与产科杂志,2017,33(8):777-778.
[5]Melkumyan AR,Priputnevich TV,Ankirskaya AS,et al.Effects of antibiotic treatment on the lactobacillus composition of vaginal microbiota[J].Bull Exp Biol Med,2015,158(6):766-768.
[6]Kuroda K,Yamashita M,Murahata Y,et al.Use of ozonated water as a new therapeutic approach to solve current concerns around antitumor treatment[J].Exp Ther Med,2018,16(3):1597-1602.
[7]Mirmortazavi A,Rajati HH,Fata A,et al.Kinetics of antifungal activity of home-generated ozonated water on Candida albicans[J].Curr Med Mycol,2018,4(2):27-31.
[8]Nogales CG,Ferrari PH,Kantorovich EO,et al.Ozone therapy in medicine and dentistry[J].J Contemp Dent Pract,2008,9(4):75-84.
[9]Almaz ME,Sonmez IS.Ozone therapy in the management and prevention of caries[J].J Formos Med Assoc,2015,114(1):3-11.
[10]Tara F,Zand-Kargar Z,Rajabi O,et al.The effects of ozonated olive oil and clotrimazole cream for treatment of vulvovaginal candidiasis[J].Altern Ther Health Med,2016,22(4):44-49.
[11]Cardia M.Treatment of vaginal trichomoniasis by ozone[J].C RSoc Fr Gyncol,1956,26(1):28-33.
[12]袁懿翀,瑞娜,冯丽华.臭氧水溶液治疗阴道炎的临床研究进展[J].中国妇幼保健,2008,23(3):420-422.
[13]鲁建云,李苗苗,高丽华,等.臭氧水浓度衰减及其杀菌作用[J].中南大学学报(医学版),2018,43(2):143-146.
[14]中华医学会妇产科学分会感染性疾病协作组.阴道微生态评价的临床应用专家共识[J].中华妇产科杂志,2016,51(10):721-723.
[15]Chu TY,Chang YC,Ding DC.Cervicovaginal secretions protect from human papillomavirus infection:effects of vaginal douching[J].Taiwan J Obstet Gynecol,2013,52(2):241-245.
[16]Schmitt C,Sobel JD,Meriwether C.Bacterial vaginosis:treatment with clindamycin cream versus oral metronidazole[J].Obstet Gynecol,1992,79(6):1020-1023.
[2]Antonio MA,Hawes SE,Hillier SL.The identification of vaginal lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species[J].J Infect Dis,1999,180(6):1950-1956.
[3]Ravel J,Gajer P,Abdo Z,et al.Vaginal microbiome of reproductiveage women[J].Proc Natl Acad Sci U S A,2011,108(Suppl1):4680-4687.
[4]廖秦平,牛小溪.人体微生态研究现状与展望[J].中国实用妇科与产科杂志,2017,33(8):777-778.
[5]Melkumyan AR,Priputnevich TV,Ankirskaya AS,et al.Effects of antibiotic treatment on the lactobacillus composition of vaginal microbiota[J].Bull Exp Biol Med,2015,158(6):766-768.
[6]Kuroda K,Yamashita M,Murahata Y,et al.Use of ozonated water as a new therapeutic approach to solve current concerns around antitumor treatment[J].Exp Ther Med,2018,16(3):1597-1602.
[7]Mirmortazavi A,Rajati HH,Fata A,et al.Kinetics of antifungal activity of home-generated ozonated water on Candida albicans[J].Curr Med Mycol,2018,4(2):27-31.
[8]Nogales CG,Ferrari PH,Kantorovich EO,et al.Ozone therapy in medicine and dentistry[J].J Contemp Dent Pract,2008,9(4):75-84.
[9]Almaz ME,Sonmez IS.Ozone therapy in the management and prevention of caries[J].J Formos Med Assoc,2015,114(1):3-11.
[10]Tara F,Zand-Kargar Z,Rajabi O,et al.The effects of ozonated olive oil and clotrimazole cream for treatment of vulvovaginal candidiasis[J].Altern Ther Health Med,2016,22(4):44-49.
[11]Cardia M.Treatment of vaginal trichomoniasis by ozone[J].C RSoc Fr Gyncol,1956,26(1):28-33.
[12]袁懿翀,瑞娜,冯丽华.臭氧水溶液治疗阴道炎的临床研究进展[J].中国妇幼保健,2008,23(3):420-422.
[13]鲁建云,李苗苗,高丽华,等.臭氧水浓度衰减及其杀菌作用[J].中南大学学报(医学版),2018,43(2):143-146.
[14]中华医学会妇产科学分会感染性疾病协作组.阴道微生态评价的临床应用专家共识[J].中华妇产科杂志,2016,51(10):721-723.
[15]Chu TY,Chang YC,Ding DC.Cervicovaginal secretions protect from human papillomavirus infection:effects of vaginal douching[J].Taiwan J Obstet Gynecol,2013,52(2):241-245.
[16]Schmitt C,Sobel JD,Meriwether C.Bacterial vaginosis:treatment with clindamycin cream versus oral metronidazole[J].Obstet Gynecol,1992,79(6):1020-1023.