不同品种升麻的HPLC指纹图谱研究
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摘要
目的研究3个不同品种升麻的指纹图谱。方法采用YMC-Pack-ODS-C18(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;流速:1.0mL·min~(-1);柱温:30℃;检测波长:320nm;进样量:10μL。结果建立了3个不同品种升麻的指纹图谱,分别鉴别出兴安升麻特征峰17个,大三叶升麻特征峰19个,升麻特征峰23个。结论该方法重复性好,简单可靠,为升麻药材定性鉴别提供理论基础。
OBJECTIVE To study the fingerprints of three different species of Cimicifuga Rhizoma. METHODS YMC-Pack-ODS-C18 column(250 mm×4.6 mm, 5 μm) was taken for study, the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at the flow rate of was 1.0 mL·min~(-1), the column temperature was 30 ℃, the detection wavelength was 320 nm and the injection volume was 10 μL. RESULTS The fingerprints of three different species of Cinicifuga Rhizoma were established. Seventeen characteristic peaks of Cimicifuga dahurica(Turcz.) Maxim, 19 characteristic peaks of Cimicifuga heracleifolia Kom. and 23 characteristic peaks of Cimicifuga foetida L. were pointed out, respectively.CONCLUSION This method is reproducible, simple and reliable, can provide a theoretical basis for the qualitative identification of Cinicifuga Rhizoma.
OBJECTIVE To study the fingerprints of three different species of Cimicifuga Rhizoma. METHODS YMC-Pack-ODS-C18 column(250 mm×4.6 mm, 5 μm) was taken for study, the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at the flow rate of was 1.0 mL·min~(-1), the column temperature was 30 ℃, the detection wavelength was 320 nm and the injection volume was 10 μL. RESULTS The fingerprints of three different species of Cinicifuga Rhizoma were established. Seventeen characteristic peaks of Cimicifuga dahurica(Turcz.) Maxim, 19 characteristic peaks of Cimicifuga heracleifolia Kom. and 23 characteristic peaks of Cimicifuga foetida L. were pointed out, respectively.CONCLUSION This method is reproducible, simple and reliable, can provide a theoretical basis for the qualitative identification of Cinicifuga Rhizoma.
引文
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[3]ZENG H L,LIU Z L,SONG Z Q,et al.Study on HPLCfingerprint and chemical constituent difference of different species of Aurantii Fructus Immaturus[J].China J Chin Mater Med(中国中药杂志),2016,41(17):3272-3278.
[4]XU Y,LI X E,QIN M J,et al.Studies on the HPLCfingerprint of Flos rosae rugosae[J].Chin Wild Plant Res(中国野生植物资源),2010,29(4):31-33.
[5]HAO P Y,GUO L,XU L,et al.Study on HPLC fingerprint in different species of Dendrobii caulis[J].Pharm Clin Chin Mater Med(中药与临床),2013,4(2):1-3.
[6]中国药典.一部[S].2015:73.
[7]高璟春,张金超,朱国元,等.升麻族植物药理活性研究进展[J].中草药,2006,37(10):5-8.
[8]GAO J C,ZHANG J C,ZHU G Y,et al.Advances in studies on pharmacological activity of tribe Cimicifugeae[J].Chin Tradit Herb Drugs(中草药),2006,37(10):5-8.
[9]王微,康云鹰.论中药指纹图谱利弊及其应用前景[J].现代中医药,2009,3(2):70-72.
[10]YAO L W,LIU Y,ZHENG X W,et al.Application of the fingerprint and characteristic fingerprint technology in traditional Chinese medicinal materials and Chinese patent medicine[J].Chin J New Drugs(中国新药杂志),2018,27(8):934-939.
[11]YANG Z F,DENG W,ZONG L,et al.HPLC fingerprint of the different producing areas and different varieties of Gastrodia elata[J].West China J Pharm Sci(华西药学杂志),2016,31(2):188-191.
[12]WANG B,ZHANG Z Q,SUN Y T,et al.HPLC fingerprint of Cimicifuga and its processed products[J].Chin Tradit Pat Med(中成药),2011,33(7):1106-1109.
[13]GONG D D,DONG J J,SUN G X,et al.A novel pattern for predicting the quality of Chinese herba preparation intelligently by high performance liquid chromatographic formula fingerprints[J].Chin J Chromatogr(色谱),2017,35(6):643-649.
[14]SUN B,SUN T.Application and analysis of fingerprint in traditional Chinese medicine by HPLC[J].Lishizhen Med Mater Med Res(时珍国医国药),2006,17(1):109-110.