利用籼稻RIL分析穗颈瘟与主要农艺性状相关性
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摘要
明确稻瘟病抗性与水稻主要农艺性状的相关性,对于抗源改良及新抗源的创制具有指导意义。以重组自交系C55为材料,连续2年在水尾病圃通过田间自然诱发稻瘟病考察C55各家系的颈瘟病情指数,在泸县考察C55各家系的主要农艺性状,分析颈瘟病指与主要农艺性状的相关性;选取田间最高颈瘟病指<23.0%的15个家系组成抗病组,选取单株粒重>34.0g的15个家系组成感病组,通过t检验比较2组间主要性状的差异显著性。结果表明:播始天数、单株穗数及株高与颈瘟病指的简单相关呈极显著负相关(P<0.01),穗实粒数、穗着粒数、千粒重及单穗重与颈瘟病指的简单相关呈极显著正相关(P<0.01);播始天数、株高及结实率与颈瘟病指的偏相关呈极显著负相关(P<0.01),单穗重与颈瘟病指的偏相关呈极显著正相关(P<0.01)。抗、感2组间颈瘟病指、穗实粒数、穗着粒数、单穗重及单株粒重的差异达5%或1%显著水平。单株产量高的家系其抗瘟性相对弱,15个抗病组家系可作为抗源利用,并对抗源改良方式进行了讨论。
Rice blast was one of the most important diseases affecting rice yield.Using varieties resistant to rice blast in production was the most effective,economic and environmental protection measures to prevent and control rice blast.The transformation of resistance sources had become an important link in rice blast resistance breeding.The relationship between rice blast resistance and main agronomic traits was significant for the creation and improvement of new resistance sources.Using the recombinant inbred line C55 as the material,the neck blast disease index of C55 families was investigated by natural induction in Shuiwei disease nursery for 2 years.The main agronomic characters of C55 families were investigated in Luxian County,and the correlation between neck blast disease index and main agronomic characters was analyzed.Fifteen families with highest blast disease index<23.0% in the fields and 15 families with grain weight >34.0 g per plant were divided into disease-resistant and susceptible groups.T test was used to compare the differences of main characters between the two groups.The results showed that:by simple correlation analysis,the days from sowing to beginning ear,panicle number per plant and the plant height were significantly negatively correlated with neck blast disease index(P<0.01).There were a significant positive correlation between the grains per panicle,filled grains per panicle,1000-grain weight and the weight of single panicle with neck blast disease index(P<0.01).Partial correlation analysis showed that:the days from sowing to beginning ear,plant height and seed setting rates were negatively correlated with neck blast disease index(P<0.01),There was a significant positive correlation between the weight of single panicle with neck blast disease index(P<0.01).The results of comprehensive correlation analysis showed that the agronomic characters were precocious,short stem,and large panicle,and large grain type materials were more susceptible to rice blast.Late maturing,high stem,spikelet and small grain type materials had relatively strong resistance to rice blast.The neck blast disease index,grains per panicle,filled grains per panicle,the weight of single spike and grain weight per plant were 5% or 1% significant difference between the two groups.The spike of resistant group was smaller than that of susceptible group,and the yield per plant was lower than that of susceptible group.The families with high yield per plant had relatively weak resistance to blast.Fifteen families with high resistance to rice blast were identified for 2 years,which can be used as a new source of resistance in rice blast breeding.Resistant material improvement were also discussed in the paper.
Rice blast was one of the most important diseases affecting rice yield.Using varieties resistant to rice blast in production was the most effective,economic and environmental protection measures to prevent and control rice blast.The transformation of resistance sources had become an important link in rice blast resistance breeding.The relationship between rice blast resistance and main agronomic traits was significant for the creation and improvement of new resistance sources.Using the recombinant inbred line C55 as the material,the neck blast disease index of C55 families was investigated by natural induction in Shuiwei disease nursery for 2 years.The main agronomic characters of C55 families were investigated in Luxian County,and the correlation between neck blast disease index and main agronomic characters was analyzed.Fifteen families with highest blast disease index<23.0% in the fields and 15 families with grain weight >34.0 g per plant were divided into disease-resistant and susceptible groups.T test was used to compare the differences of main characters between the two groups.The results showed that:by simple correlation analysis,the days from sowing to beginning ear,panicle number per plant and the plant height were significantly negatively correlated with neck blast disease index(P<0.01).There were a significant positive correlation between the grains per panicle,filled grains per panicle,1000-grain weight and the weight of single panicle with neck blast disease index(P<0.01).Partial correlation analysis showed that:the days from sowing to beginning ear,plant height and seed setting rates were negatively correlated with neck blast disease index(P<0.01),There was a significant positive correlation between the weight of single panicle with neck blast disease index(P<0.01).The results of comprehensive correlation analysis showed that the agronomic characters were precocious,short stem,and large panicle,and large grain type materials were more susceptible to rice blast.Late maturing,high stem,spikelet and small grain type materials had relatively strong resistance to rice blast.The neck blast disease index,grains per panicle,filled grains per panicle,the weight of single spike and grain weight per plant were 5% or 1% significant difference between the two groups.The spike of resistant group was smaller than that of susceptible group,and the yield per plant was lower than that of susceptible group.The families with high yield per plant had relatively weak resistance to blast.Fifteen families with high resistance to rice blast were identified for 2 years,which can be used as a new source of resistance in rice blast breeding.Resistant material improvement were also discussed in the paper.
引文
[1]孙国昌,杜新法,陶荣祥,等.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报,1998,28(4):289-292.
[2]陈德西,李仕贵,马炳田,等.水稻稻瘟病育种研究进展[J].安徽农学通报,2008,14(17):206-209.
[3]袁筱萍,魏兴华,余汉勇,等.部分中国栽培稻资源对稻瘟病的抗性分析[J].植物保护,2005,31(5):27-31.
[4]徐灵超,刘二明,黄金杯,等.湖南省103个水稻品种的抗瘟基因型鉴定[J].湖南农业大学学报(自然科学版),2009,35(1):17-20.
[5]李培富,史晓亮,王建飞,等.太湖流域粳稻地方品种黑壳子粳稻抗稻瘟病基因的分子定位[J].中国水稻科学,2007,21(6):579-584.
[6]陈葆棠,彭仲明,徐运启.水稻品种矮梅早3号抗稻瘟病的遗传[J].遗传学报,1993,20(4):354-361.
[7]雷财林,王玖林,毛世宏,等.籼稻品种窄叶青8号抗瘟病基因分析[J].遗传学报,1997,24(1):36-41.
[8]张晋,谢戎,黄富,等.四川水稻抗稻瘟病种质资源农艺性状的遗传改良[J].西南农业学报,2007,20(5):899-906.
[9]郑艳,谢戎,刘成元,等.72份广东水稻种质资源的抗瘟性及农艺性状评价[J].四川农业大学学报,2008,26(2):197-201.
[10]况浩池,曾正明,罗俊涛,等.“十一五”四川审定杂交稻组合产量和稻瘟病抗性分析[J].安徽农业科学,2011,39(27):16503-16506.
[11]曾宪平,何芳,吕建群,等.四川省2001-2010年审定的杂交中稻迟熟品种的农艺性状分析[J].西南师范大学学报(自然科学版),2010,37(4):91-95.
[12]金洪梅,王欢.对我国超级稻品种抗瘟性初步分析与评价[J].中国稻米,2011,17(5):35-37.
[13]Khan M A,Bhuiyan M R,Hossain M S,et al.Neck blast disease influences grain yield and quality traits of aromatic rice[J].C RBiol,2014,337(11):635-641.
[14]潘国君,陈书强,宋成艳,等.寒地早粳品种稻瘟病抗性与产量性状的关系[J].中国农学通报,2009,25(19):236-238.
[15]袁筱萍,徐群,余汉勇,等.国际稻瘟病圃种质稻瘟病抗性的鉴定与评价[J].中国稻米,2011,17(2):11-13.
[16]林宏博.水稻稻瘟病田间抗性与部分农艺性状的关系[J].作物杂志,1988(2):33-34.
[17]欧巧明.单粒传法建立重组自交系之方法[EB/OL].http://blog.sciencenet.cn/home.php?do=blog&id=402036&mod=space&uid=434603,2011.
[18]谢戎,刘成元,李永洪,等.转GO基因水稻杂交后代对稻瘟病及纹枯病的田间评价[J].西南农业学报,2018,31(5):965-972.
[19]郭龙彪,罗利军,钟代斌,等.籼粳交重组自交系群体主要农艺性状分析[J].中国水稻科学,2001,15(3):221-224.
[20]杨惠杰,杨仁崔,李义珍,等.水稻超高产品种的产量潜力及产量构成因素分析[J].福建农业学报,2000,15(3):1-8.
[21]谭酬志,邹小云,熊春梅,等.籼型三系杂交水稻产量性状的相关及通径分析[J].安徽农业科学,2008,36(29):12629-12631.
[22]何秀英,王玲,吴伟怀,等.水稻稻瘟病抗性基因的定位、克隆及育种应用研究进展[J].中国农学通报,2014,30(6):1-12.
[23]Hittalmani S,Parco A,Mew T V,et al.Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J].Theor Appl Genet,2000,100:1121-1128.
[24]Narayanan N N,Baisakh N,Vera Cruz C M,et al.Molecular breeding for the development of blast and bacterial blight resistance in rice cv IR50[J].Crop Sci,2002,42:2072-2079.
[25]田大刚,苏军,陈建民,等.1092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J].分子植物育种,2012,10(2):214-221.
[26]曹立勇,占小登,庄士杰,等.利用分子标记辅助育种技术育成优质高产抗病杂交稻国稻1号[J].杂交水稻,2005,20(3):16-18.
[27]安正帅,刘国兰,梅捍卫,等.标记辅助改良节水抗旱杂交稻亲本材料的稻瘟病抗性[J].分子植物育种,2010,8(6):1172-1176.
[28]Deng Y,Zhai K,Xie Z,et al.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J].Science,2017(355):962-965.
[29]Li W,Zhu Z,Chern M,et al.A natural allele of a transcription factor in rice confers broad-spectrum blast resistance[J].Cell,2017,170(1):114-126.
[30]Zhou X G,Liao H C,Chern M,et al.Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance[J].PNAS,2018,115(12):3174-3179.
[2]陈德西,李仕贵,马炳田,等.水稻稻瘟病育种研究进展[J].安徽农学通报,2008,14(17):206-209.
[3]袁筱萍,魏兴华,余汉勇,等.部分中国栽培稻资源对稻瘟病的抗性分析[J].植物保护,2005,31(5):27-31.
[4]徐灵超,刘二明,黄金杯,等.湖南省103个水稻品种的抗瘟基因型鉴定[J].湖南农业大学学报(自然科学版),2009,35(1):17-20.
[5]李培富,史晓亮,王建飞,等.太湖流域粳稻地方品种黑壳子粳稻抗稻瘟病基因的分子定位[J].中国水稻科学,2007,21(6):579-584.
[6]陈葆棠,彭仲明,徐运启.水稻品种矮梅早3号抗稻瘟病的遗传[J].遗传学报,1993,20(4):354-361.
[7]雷财林,王玖林,毛世宏,等.籼稻品种窄叶青8号抗瘟病基因分析[J].遗传学报,1997,24(1):36-41.
[8]张晋,谢戎,黄富,等.四川水稻抗稻瘟病种质资源农艺性状的遗传改良[J].西南农业学报,2007,20(5):899-906.
[9]郑艳,谢戎,刘成元,等.72份广东水稻种质资源的抗瘟性及农艺性状评价[J].四川农业大学学报,2008,26(2):197-201.
[10]况浩池,曾正明,罗俊涛,等.“十一五”四川审定杂交稻组合产量和稻瘟病抗性分析[J].安徽农业科学,2011,39(27):16503-16506.
[11]曾宪平,何芳,吕建群,等.四川省2001-2010年审定的杂交中稻迟熟品种的农艺性状分析[J].西南师范大学学报(自然科学版),2010,37(4):91-95.
[12]金洪梅,王欢.对我国超级稻品种抗瘟性初步分析与评价[J].中国稻米,2011,17(5):35-37.
[13]Khan M A,Bhuiyan M R,Hossain M S,et al.Neck blast disease influences grain yield and quality traits of aromatic rice[J].C RBiol,2014,337(11):635-641.
[14]潘国君,陈书强,宋成艳,等.寒地早粳品种稻瘟病抗性与产量性状的关系[J].中国农学通报,2009,25(19):236-238.
[15]袁筱萍,徐群,余汉勇,等.国际稻瘟病圃种质稻瘟病抗性的鉴定与评价[J].中国稻米,2011,17(2):11-13.
[16]林宏博.水稻稻瘟病田间抗性与部分农艺性状的关系[J].作物杂志,1988(2):33-34.
[17]欧巧明.单粒传法建立重组自交系之方法[EB/OL].http://blog.sciencenet.cn/home.php?do=blog&id=402036&mod=space&uid=434603,2011.
[18]谢戎,刘成元,李永洪,等.转GO基因水稻杂交后代对稻瘟病及纹枯病的田间评价[J].西南农业学报,2018,31(5):965-972.
[19]郭龙彪,罗利军,钟代斌,等.籼粳交重组自交系群体主要农艺性状分析[J].中国水稻科学,2001,15(3):221-224.
[20]杨惠杰,杨仁崔,李义珍,等.水稻超高产品种的产量潜力及产量构成因素分析[J].福建农业学报,2000,15(3):1-8.
[21]谭酬志,邹小云,熊春梅,等.籼型三系杂交水稻产量性状的相关及通径分析[J].安徽农业科学,2008,36(29):12629-12631.
[22]何秀英,王玲,吴伟怀,等.水稻稻瘟病抗性基因的定位、克隆及育种应用研究进展[J].中国农学通报,2014,30(6):1-12.
[23]Hittalmani S,Parco A,Mew T V,et al.Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice[J].Theor Appl Genet,2000,100:1121-1128.
[24]Narayanan N N,Baisakh N,Vera Cruz C M,et al.Molecular breeding for the development of blast and bacterial blight resistance in rice cv IR50[J].Crop Sci,2002,42:2072-2079.
[25]田大刚,苏军,陈建民,等.1092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J].分子植物育种,2012,10(2):214-221.
[26]曹立勇,占小登,庄士杰,等.利用分子标记辅助育种技术育成优质高产抗病杂交稻国稻1号[J].杂交水稻,2005,20(3):16-18.
[27]安正帅,刘国兰,梅捍卫,等.标记辅助改良节水抗旱杂交稻亲本材料的稻瘟病抗性[J].分子植物育种,2010,8(6):1172-1176.
[28]Deng Y,Zhai K,Xie Z,et al.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J].Science,2017(355):962-965.
[29]Li W,Zhu Z,Chern M,et al.A natural allele of a transcription factor in rice confers broad-spectrum blast resistance[J].Cell,2017,170(1):114-126.
[30]Zhou X G,Liao H C,Chern M,et al.Loss of function of a rice TPR-domain RNA-binding protein confers broad-spectrum disease resistance[J].PNAS,2018,115(12):3174-3179.