不同试验周期下Cu对玉米种子发芽和根伸长的影响
摘要
为探究植物种子是否会因为受试物暴露时间不同而得到不同的发芽抑制率、根长下降率及EC_(50),进而影响受试物生态毒理学危害性级别的判定,选用玉米为试验种子,CuSO_4·5H_2O为受试物,设置了6个受试物浓度组,开展了周期为2~7d的试验。结果表明:在中低暴露浓度下,不同周期对玉米种子发芽率影响较大,发芽抑制率的EC_(50)最值相差高达352.9mg/L,也使根长出现显著性差异,但根长下降率及其EC_(50)无明显差异。得出了试验周期不同,不影响CuSO_4·5H_2O生态毒理学危害性分级结果的结论。
引文
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[3]Ting Ting Liu,Peng Wu,Li Hong Wang.Response of soybean seed germination to cadmium and acid rain[J].Biol Trace Elem Res,2011,144(13):1186~1196.
[4]OPPTS.Ecological Effects Test Guidelines,OPPTS 850.4200Seed Germination/Root Elongation Toxicity Test[S].US EPA,1996.
[5]中华人民共和国环境保护部.新化学物质申报登记指南[S].北京:中国标准出版社,2010.
[6]OPPTS.Ecological Effects Test Guidelines,OPPTS 850.4200Seed Germination/Root Elongation Toxicity Test[S].US EPA,1996.
[7]ISO 10390:2005.Soil Quality-Determination of pH[S].[s.l.]、[s.n.].2005.
[8]浙江农业大学种子教研组.种子检验简明教程[M].北京:农业出版社.1980.
[9]高柱,王小玲,刘腾云,等.重金属Cu污染对苏丹草种子发芽及幼苗生长的影响[J].中国农学通报.2013,29(25):199~204.
[10]张景光,王新平,李新荣,等.荒漠植物生活史对策研究进展与展望[J].中国沙漠.2005,25(3):306~313.
[11]金彩霞,郝苗青,王庆纬,等.利巴韦林对4种作物种子发芽的影响[J].生态毒理学报.2013,8(1):37~41.
[12]宋玉芳,许华夏,任丽萍,等.土壤重金属对白菜种子发芽与根伸长抑制的生态毒性效应[J].环境科学.2002,23(1):103~107.
[13]Liu X L,Zhang S Z,Shan X Q,et al.Toxicity of arsenate and arsenite on germination seedling growth and amylolytic activity of wheat[J].Chemosphere,2005,61(13):293~301
[14]Kapustka L,Lipton J,Galbraith H,et al.Metal and arsenic impacts to soils,vegetation communities and wild-life habitat in southwest Montana up lands contaminated by smelter emissions.Ⅱ.Laboratory phytotoxicity studies[J].Environmental Txicology and Chemistry,1995(14):1905~1912
[15]Kjaer C,Pedersen N,Elmegaard N.Effects of soil copper on black bindweed(Fallopia convovulus)in the laboratory and in the field.Arch.Environ.Contamn[J].Toxicol.,1998,35(4):14~19.
[16]国家环境保护总局.新化学物质危害性鉴别导则:HJ/T154-2004[S].北京:中国标准出版社,2004.
[17]聂晶磊,周红,刁树林,等.新化学物质环境管理与生态毒理学[J].毒理学杂志,2005,19(1):12~14.