铜-磺胺嘧啶复合胁迫对蔬菜种子发芽的急性毒性效应
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摘要
研究了黄潮土中常用兽药磺胺嘧啶(SD)与重金属铜(Cu)单一及复合污染对小白菜和西红柿种子发芽(发芽率、根伸长、芽伸长)的影响,分析了土壤中药物浓度与作物生长抑制的剂量-效应关系及复合污染的毒性效应。结果表明,无论是在Cu或SD单一污染物作用下,根伸长和芽伸长抑制率与污染物浓度显著相关(P<0.01),发芽抑制率与污染物浓度不相关(P>0.05);污染物对根伸长及芽伸长的抑制高于对种子发芽的抑制;SD对2种作物的毒性效应明显强于Cu,SD对小白菜和西红柿根伸长的ID50(抑制率为50%时污染物浓度)分别为1.7和33.6 mg·kg~(-1),而Cu为273.6和457.7 mg·kg~(-1)。Cu-SD复合污染,对西红柿的根和芽伸长的拮抗作用显著(P<0.01);但对小白菜来说,在低浓度Cu(100 mg·kg~(-1))作用下,二者的联合拮抗作用不显著(P>0.05),随Cu的增加,拮抗作用显著(P<0.01)。
This study reported the single and joint effects of commonly used sulfadiazine(SD) veterinary drug and copper(Cu) on the seed germination(including germination rate, root and shoot elongation) of pakchoi and tomato in yellow fluvo-aquic soil, analyzed the dose-response relationship between the drug concentration in soil and inhibitory action on crop growth, as well as the toxic effect of combined pollution. The results indicated that the inhibitory rate of root and shoot elongation correlated significantly with pollutant concentration(P <0.01), the inhibitory rate of germination and pollutant concentration were uncorrelated(P >0.05), whether in the presence of individual Cu or SD; the inhibitory effect of pollutants on root and shoot elongation was much stronger than that of the seed germination; the toxic effect of SD was much stronger than that of Cu, the ID50(half inhibition concentration)values of SD on root elongation of pakchoi and tomato were 1.7 and 33.6 mg·kg~(-1), respectively, while Cu were273.6 and 457.7 mg·kg~(-1). The antagonism effect of Cu-SD combined pollution on the root and shoot elongation of the tomato was remarkable(P <0.01); but for the pakchoi, the combined antagonism effect was not significant when the Cu concentration was lower than 100 mg·kg~(-1)(P >0.05), the antagonism effect was gradually notable as the increasing of the Cu concentration(P <0.01).
This study reported the single and joint effects of commonly used sulfadiazine(SD) veterinary drug and copper(Cu) on the seed germination(including germination rate, root and shoot elongation) of pakchoi and tomato in yellow fluvo-aquic soil, analyzed the dose-response relationship between the drug concentration in soil and inhibitory action on crop growth, as well as the toxic effect of combined pollution. The results indicated that the inhibitory rate of root and shoot elongation correlated significantly with pollutant concentration(P <0.01), the inhibitory rate of germination and pollutant concentration were uncorrelated(P >0.05), whether in the presence of individual Cu or SD; the inhibitory effect of pollutants on root and shoot elongation was much stronger than that of the seed germination; the toxic effect of SD was much stronger than that of Cu, the ID50(half inhibition concentration)values of SD on root elongation of pakchoi and tomato were 1.7 and 33.6 mg·kg~(-1), respectively, while Cu were273.6 and 457.7 mg·kg~(-1). The antagonism effect of Cu-SD combined pollution on the root and shoot elongation of the tomato was remarkable(P <0.01); but for the pakchoi, the combined antagonism effect was not significant when the Cu concentration was lower than 100 mg·kg~(-1)(P >0.05), the antagonism effect was gradually notable as the increasing of the Cu concentration(P <0.01).
引文
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[19]张娟,郭文录,孙成.磺胺嘧啶在黑土及其不同粒径组分中的吸附行为研究[J].农业环境科学学报,2011,30(2):301-306Zhang J,Guo W L,Sun C.Asorption behavior of sulfadi-azine on black soil and its different particle sizes[J].Journal of Agro-Environment Science,2011,30(2):301-306
[20]Inaba S,Takenaka C.Effects of dissolved organic matter on toxicity and bioavailability of copper for lettuce sprouts[J].Environment International,2005,31:603-608
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[22]霍燕兰.药物分析技术[M].北京:化学工业出版社,2005Huo Y L.Drug Analysis Technology[M].Beijing:Chemical Industry Press,2005
[23]金彩霞,刘军军,鲍林林,等.磺胺间甲氧嘧啶-镉复合污染对作物种子发芽的影响[J].中国环境科学,2010,30(6):839-844Jin C X,Liu J J,Bao L L,et al.Joint toxicity of sulfamonomethoxine and Cd on seed germination and root elongation of crops in soil[J].China Environmental Science,2010,30(6):839-844
[24]周启星,程云,张倩茹,等.复合污染生态毒理效应的定量分析关系[J].中国科学(C辑),2003,33(6):566-573Zhou Q X,Cheng Y,Zhang Q R,et al.Quantitative analysis of the relationship between ecotoxicological effects of complex pollution[J].Science China,2003,33(6):566-573◆
[2]Wu T,Li S F,Dai G,et al.Research process of veterinary drug residues of fluoroquinolones in animal food[J].China Animal Health,2012(3):19-22
[3]Boxall A B A,Johnson P,Smith E J,et al.Uptake of veterinary medicines from soils into crops[J].Journal of Agricultural and Food Chemistry,2006,54(6):2288-2297
[4]Burkhardt M,Stoob K,Stamm C,et al.Veterinary antibiotics in animal slurries-A new environmental issue in grassland research[J].Grassland Science in Europe,2004,9:322-324
[5]邰义萍,罗晓栋,莫测辉,等.广东省畜牧粪便中喹诺酮类和磺胺类抗生素的含量与分布特征研究[J].环境科学,2011,32(4):1188-1193Tai Y P,Luo X D,Mo C H,et al.Occurrence of quinolone and sulfonamide antibiotics in swine and cattle manures from large-scale feeding operation of Guanggong Province[J].Environmental Science,2011,32(4):1188-1193(in Chinese)
[6]刘锋,廖德润,李可,等.畜禽养殖基地磺胺类喹诺酮类和大环内酯类抗生素污染特征[J].农业环境科学学报,2013,32(4):847-853Liu F,Miu D R,Li K,et al.Pollution characteris of the sulfonamindes,quinolones and macrolides in the samples collected from livestock and poultry feedlots[J].Journal of Agro-Environment Science,2013,32(4):847-853(in Chinese)
[7]王小骊,张永志,王刚军,等.蔬菜中有害重金属元素污染研究进展[J].浙江农业学报,2004,16(5):259-262Wang X L,Zhang Y Z,Wang G J,et al.Advance of study on harmful heavy metal pollution on vegetables[J].Acta Agriculture Zhejiangensis,2004,16(5):259-262(in Chinese)
[8]金彩霞,刘军军,陈秋颖,等.兽药污染土壤对小麦和白菜根伸长抑制的毒性效应[J].农业环境科学学报,2009,28(7):1358-1362Jin C X,Liu J J,Chen Q Y,et al.Toxicological effects of veterinary drugs in soil on the inhibition of root elongation of wheat and Chinese cabbages[J].Journal of AgroEnvironment Science,2009,28(7):1358-1362(in Chinese)
[9]Jin C X,Chen Q Y,Sun R L,et al.Ecotoxic effects of sulfadiazine sodium,sulfamonomethoxine sodium and enrofloxacin on wheat,Chinese cabbage and tomato[J].Ecotoxicology,2009,18:878–885
[10]薛艳,沈振国,周东美.蔬菜对土壤重金属吸收的差异与机理[J].土壤,2005,37(1):32-36Xue Y,Shen Z G,Zhou D M.Difference in heavy mental uptake between various vegetables and its mechanism[J].Soil,2005,37(1):32-36(in Chinese)
[11]郑爱珍,刘传平,沈振国.铜对白菜、青菜生长的影响[J].北方园艺,2005(2):42-43Zheng A Z,Liu C P,Shen Z G.Influences of copper on cabbage,and Chinese cabbage grown[J].Northern Horticulture,2005(2):42-43(in Chinese)
[12]钱晓薇.硫酸铜对蚕豆根尖细胞有丝分裂的影响[J].细胞生物学杂志,2004,26(1):171-175Qian X W.The effect of copper sulphate on mitosis of Vicia faba root tip cells[J].Chinese Journal of Cell Biology,2004,26(1):171-175(in Chinese)
[13]Arduini I,Godbold D L,Onnis A.Cadmium and copperchange root growth and morphology of Pinus pinea and Pinus pinaster seedlings[J].Physiologia Plantarum,1994,92(4):675-680
[14]Liu T F,Wang T,Sun C,et al.Single and joint toxicity of cypermethrin and copper on Chinese cabbage(Pakchoi)seeds[J].Journal of Hazardous Materials,2009,163:344–348
[15]Stewart A R,Malley D F.Effect of metal mixture(Cu,Zn,Pb and Ni)on cadmium partitioning in littoral sediments and its accumulation by the freshwater macrophytc Eriocaùlon septangulàre[J].Environmental Toxicology and Chemistry,1999,18(3):436-447
[16]Sharma S S,Schat H,Vooijs R,et al.Combination toxicology of copper,zinc,and cadmium in binary mixtures:Concentration dependent antagonistic,nonadditive,and synergistic effects on root growth in Silene vulgaris[J].Environmental Toxicology and Chemistry,1999,18(2):348-355
[17]王米道,程凤侠,司友斌.铜与草甘膦复合污染对小麦种子发芽与根伸长的抑制作用[J].生态毒理学报,2009,4(4):591-596Wang M D,Cheng F X,Si Y B.The inhibition of the combined pollution of copper and glyphosate to the seed germination and root elongation of wheat[J].Asian Journal of Ecotoxicology,2009,4(4):591-596
[18]Chao L,Zhou Q X,Chen S,et al.Single and joint stress of acetochlor and Pb on three agricultural crops in Northeast China[J].Journal of Environmental Science,2007,19:719-724
[19]张娟,郭文录,孙成.磺胺嘧啶在黑土及其不同粒径组分中的吸附行为研究[J].农业环境科学学报,2011,30(2):301-306Zhang J,Guo W L,Sun C.Asorption behavior of sulfadi-azine on black soil and its different particle sizes[J].Journal of Agro-Environment Science,2011,30(2):301-306
[20]Inaba S,Takenaka C.Effects of dissolved organic matter on toxicity and bioavailability of copper for lettuce sprouts[J].Environment International,2005,31:603-608
[21]Chauhan L K S,Gupta S K.Combined cytogenetic and ultrastructural effects of substituted urea herbicides and synthetic pyrethroid insecticide on the root meristem cells of Allium cepa[J].Pesticide Biochemistry and Physiology,2005,82:27–35
[22]霍燕兰.药物分析技术[M].北京:化学工业出版社,2005Huo Y L.Drug Analysis Technology[M].Beijing:Chemical Industry Press,2005
[23]金彩霞,刘军军,鲍林林,等.磺胺间甲氧嘧啶-镉复合污染对作物种子发芽的影响[J].中国环境科学,2010,30(6):839-844Jin C X,Liu J J,Bao L L,et al.Joint toxicity of sulfamonomethoxine and Cd on seed germination and root elongation of crops in soil[J].China Environmental Science,2010,30(6):839-844
[24]周启星,程云,张倩茹,等.复合污染生态毒理效应的定量分析关系[J].中国科学(C辑),2003,33(6):566-573Zhou Q X,Cheng Y,Zhang Q R,et al.Quantitative analysis of the relationship between ecotoxicological effects of complex pollution[J].Science China,2003,33(6):566-573◆