茯苓固态发酵产物对益生菌增殖效果初探
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摘要
通过测定菌体培养液吸光值的方法,进行茯苓发酵产物提取液(FPE)对乳杆菌和双歧杆菌的增殖效果体外实验。结果表明:与异麦芽低聚糖(IMO)和低聚果糖(FOS)相比,添加了FPE的培养基可以更好的促进双歧杆菌的生长(P <0.01),说明FPE可以作为一种双歧因子,促进双歧杆菌的增殖。培养基中FPE的浓度为2.5%～5%时对双歧杆菌和乳杆菌的增殖效果最好。在添加了FPE的培养基中生长的双歧杆菌的生长迟缓期较在未添加的基础培养基中更短,添加FPE的培养液的吸光值明显大于未添加培养液(P<0.01),说明发酵产物提取液可以作为一种有效的益生元促进二者生长。
The effect on the proliferation of Bifidobacterium and Lactobacillus by extracts from poria cocos solidfermentation products(FPE) was tested in vitro. The results demonstrated FPE could be a bifidusfactor to promote the proliferation of Bifidobacterium. Compared with other bifidusfactors,such as isomalto oligosaccharides and fructo oligosaccharides,FPE could significantly promote the growth of Bifidobacterium(P < 0.01). High concentration of FPE inhibited the proliferation of Bifidobacterium and Lactobacillus. The best concentration of FPE was from 2.5% to 5%. The absorbance of culture liquid containing FPE was higher than the control group's(P < 0.01). Therefore,the extract can be used as a prebiotic to promote the growth of Bifidobacterium and Lactobacillus.
The effect on the proliferation of Bifidobacterium and Lactobacillus by extracts from poria cocos solidfermentation products(FPE) was tested in vitro. The results demonstrated FPE could be a bifidusfactor to promote the proliferation of Bifidobacterium. Compared with other bifidusfactors,such as isomalto oligosaccharides and fructo oligosaccharides,FPE could significantly promote the growth of Bifidobacterium(P < 0.01). High concentration of FPE inhibited the proliferation of Bifidobacterium and Lactobacillus. The best concentration of FPE was from 2.5% to 5%. The absorbance of culture liquid containing FPE was higher than the control group's(P < 0.01). Therefore,the extract can be used as a prebiotic to promote the growth of Bifidobacterium and Lactobacillus.
引文
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[2]Cuéllar M J,Giner Rosa M,Recio M,et.al.Two fungal lanostane derivatives as phospholipase A2 inhibitor[J].Journal of Natural Products,1996,59(10):977-979.
[3]宋克玉,江振友,严群超,等.党参及茯苓对小鼠肠道菌群调节作用的实验研究[J].中国临床药理学杂志,2011,27(2):142-145.
[4]沈思,李孚杰,梅光明,等.茯苓皮三萜类物质含量的测定及其抑菌活性的研究[J].食品科学,2009,30(1):97-100.
[5]Sekiya N,Goto H,Shimada Y,et al.Inhibitory effects of triterpenes isolated from Hoelen on free radical-induced lysis of red blood cells[J].Phytotherapy Research,2003,17(2):160-162.
[6]Huang Y C,Chang W L,Huang S F,et al.Pachymic acid stimulates glucose uptake through enhanced GLUT4 expression and translocation[J].European Journal of Pharmacology,2010,648(1-3):39-49.
[7]李永明,徐子伟,李芳,等.发酵谷物液体饲料对超早期断奶仔猪生长性能和肠道微生物菌群多样性的影响[J].动物营养学报,2010,22(6):1650-1657.
[8]刘海燕,闫晓刚,邱玉朗,等.发酵酶解豆粕对小鼠血液抗氧化指标和胃肠道消化酶活性及肠道菌群的影响[J].中国畜牧杂志,2011,47(21):41-44.
[9]张泽生,张丽,吕晓玲,等.菊粉低聚果糖与蔗糖低聚果糖对双歧杆菌体外增殖的研究[J].中国食品添加剂,2016(1):76-80.
[10]孔彦卓,尹乐斌,雷志明,张昌运,张臣飞,赵良忠.一株高产酸乳酸菌的分离鉴定及生物学特性研究[J].中国食品添加剂,2017(09):88-94.
[11]鲁庆.常用香辛料和防腐剂对益生菌增殖的影响[D].中南林业科技大学,2014.
[12]卢旭.莲子低聚糖对双歧杆菌增殖效应的研究[D].福建农林大学,2012.
[13]胡开辉,林辉,李佳欢,等.一种茯苓固态发酵型饮料及其制作方法:中国,201410577398.5[P].2014-10-27.
[14]Kaur I P,Kuhad A,Garg A,et al.Probiotics:delineation of prophylactic and therapeutic benefits.[J].Journal of Medicinal Food,2009,12(2):219.
[15]Le R C I,?t?epetova Jelena,Epp S,et al.New insights into the impact of Lactobacillus population on host-bacteria metabolic interplay:[J].Oncotarget,2015,6(31):30545-30556.
[16]Macsharry J,O’Mahony C,Shalaby K H,et al.Immunomodulatory effects of feeding with Bifidobacterium longum,on allergen-induced lung inflammation in the mouse[J].Pulmonary Pharmacology&Therapeutics,2012,25(4):325-334.
[17]Dylag K,Hubalewskamazgaj M,Surmiak M,et al.Probiotics in the mechanism of protection against gut inflammation and therapy of gastrointestinal disorders.[J].Curr Pharm Des,2014,20(7):1149-1155.
[18]Amrouche T,Boutin Y,Moroni O,et al.Production and characterization of anti-bifidobacteria monoclonal antibodies and their application in the development of an immuno-culture detection method[J].Journal of Microbiological Methods,2006,65(1):159-170.
[19]刘树兴,闫莉斐,陈蕊,等.紫苏油对益生菌的增殖作用研究[J].中国油脂,2018,43(1):61-63.
[20]Gao S,Lai C K M,Cheung P C K.Nondigestible carbohydrates isolated from medicinal mushroom sclerotia as novel prebiotics.[J].International Journal of Medicinal Mushrooms,2009,11(1):1-8.
[21]Chang C J,Lin C S,Lu C C,et al.Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota.[J].Nature Communications,2015,6:7489.
[22]冉域辰,黎海芪,刘明芳.金银花水提物对双歧杆菌与乳酸杆菌体外生长的影响[J].时珍国医国药,2008,19(5):1131-1133.