柠檬酸交联对谷朊纤维稳定性能的影响
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摘要
以谷朊粉为原料,利用湿法纺丝方法制备谷朊纤维,并用柠檬酸对谷朊纤维进行交联处理,制备出交联谷朊纤维。通过比较谷朊粉、谷朊纤维、交联谷朊纤维在热稳定性、水解稳定性、大分子分子量及其构象等方面的差异,分析了柠檬酸交联对谷朊纤维的影响。结果表明,柠檬酸交联处理可提高谷朊大分子的分子量,改变蛋白质大分子的二级结构,从而改善了谷朊纤维的热稳定性和水解稳定性。
Using gluten as raw material,gluten fibers were prepared by wet spinning method and gluten fibers were cross-linked with citric acid to prepare crosslinked gluten fibers. The effects of citric acid crosslinking on gluten fiber were analyzed by comparing the differences in thermal stability,hydrolytic stability,molecular weight and conformation of gluten,gluten fibers and crosslinked gluten fibers. The results showed that the citric acid crosslinking treatment can increase the molecular weight of gluten macromolecules and change the secondary structure of protein macromolecules,thus improving the thermal stability and hydrolytic stability of gluten fibers.
Using gluten as raw material,gluten fibers were prepared by wet spinning method and gluten fibers were cross-linked with citric acid to prepare crosslinked gluten fibers. The effects of citric acid crosslinking on gluten fiber were analyzed by comparing the differences in thermal stability,hydrolytic stability,molecular weight and conformation of gluten,gluten fibers and crosslinked gluten fibers. The results showed that the citric acid crosslinking treatment can increase the molecular weight of gluten macromolecules and change the secondary structure of protein macromolecules,thus improving the thermal stability and hydrolytic stability of gluten fibers.
引文
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[2]王可,宋义虎.交联剂改性小麦醇溶蛋白/壳聚糖复合膜的制备与性能[J].材料科学与工程学报,2013,31(1):78-83.
[3]李临生,张淑娟.戊二醛与蛋白质反应的影响因素和反应机理[J].中国皮革,1997(12):6-10.
[4] Reddy N,Yang Y. Novel protein fibers from wheat gluten[J]. Biomacromolecules,2007,8(2):638-643.
[5]温乐蛟,侯秀良,娄高源,等.谷朊纤维制备及其柠檬酸交联处理研究[J].化工新型材料,2014,42(8):101-103,107.
[6]曹红翠.紫外分光光度法测定蛋白质的含量[J].广东化工,2007,34(8):93-94.
[7]卢雁,李向荣.蛋白质变性机理与变性时的热力学参数研究进展[J].化学进展,2005(5):905-910.
[8] Marqui C. Chemical reactions in cottonseed protein crosslinking by formaldehyde,glutaraldehyde,and glyoxal for the formation of protein films with enhanced mechanical properties[J]. Journal of Agricultural and Food Chemistry,2001,49(10):4676-4681.
[9] Sun S,Song Y,Zheng Q. Morphologies and properties of thermo-molded biodegradable plastics based on glycerolplasticized wheat gluten[J]. Food Hydrocolloids,2007,21(7):1005-1013.
[10] Jiang You,He Muyi,Zhang Wenjing,et al. Recent advances of capillary electrophoresis-mass spectrometry instrumentation and methodology[J]. Chinese Chemical Letters,2017,28(8):1640-1652.
[11] Reddy N,Li Y,Yang Y. Wet cross-linking gliadin fibers with citric acid and a quantitative relationship between cross-linking conditions and mechanical properties[J].Journal of Agricultural and Food Chemistry,2009,57(1):90-98.
[12] Reddy N,Yang Y. Self-crosslinked gliadin fibers with high strength and water stability for potential medical applications[J]. Journal of Materials Science-Materials in Medicine,2008,19(5):2055-2061.