一株苏云金亚种的发酵产物对柳蚜3种保护酶活性的影响
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摘要
为探究苏云金杆菌(Bacillus thuringiensis Bt)对蚜虫的生物活性影响及作用方式,以苏云金亚种(Bacillus thuringiensis subsp.thuringiensis)为目的菌株,以发酵产物为材料。利用5种不同浓度的菌液分别喷雾处理柳蚜,72h校正死亡率为29.74%～78.88%,其浓度与及死亡率呈正相关关系。苏云金杆菌亚种发酵液和发酵液滤液处理柳蚜后,12～24h柳蚜超氧化物歧化酶(SOD)比活力均被抑制,其抑制率分别为21.06%～38.11%和4.69%～36.79%,其中18h、24h对SOD比活力的抑制达显著水平(p<0.05)。发酵液和滤液处理组18h前对柳蚜过氧化氢酶(CAT)比活力的影响总体呈激活趋势,其中发酵液和滤液处理组分别在18h、12h对柳蚜CAT的激活率最高,其CAT比活力分别为对照的1.8890倍和2.9463倍。滤液处理组对柳蚜POD比活力总体呈激活作用,其激活率为14.78%～57.39%;发酵液处理组6h和18hPOD比活力显著高于对照,其他时间点POD比活力与对照无显著差异。
To investigate the biological activity and action mechanism of Bacillus thuringiensis(Bt)on aphids,a strain of Bacillus thuringiensis subsp.thuringiensis was used as the fermentation product.The sputum was treated with 5 different concentrations of bacterial liquid,and the corrected mortality rate reached 29.74%-78.88%at 72 h.The concentration was positively correlated with mortality.After treated with the fermentation broth of Suyunjin subspecies and fermentation broth,the activity of superoxide dismutase(SOD)in the sorghum was inhibited from12 hto 24 h,and the inhibition rates were 21.06%-38.11% and 4.69%%-36.79%,respectively,At 18 hand 24 h after treatment,the inhibition of SOD activity reached a significant level(p<0.05).The effects of the fermentation broth and filtrate treatment group on the activity of catalase(CAT)were all activated before 18 h,and the fermentation broth and filtrate treatment group had the highest activation rate of CAT at 18 hand 12 h,respectively.The CAT viability was 1.8890 and 2.9463 folds of the control,respectively.The filtrate treatment group activated the activity of POD in Aphis farinosa Gmelin and the activation rate was 14.78%-57.39%.The POD activity in the fermentation broth treatment group was significantly higher than that in the control at 6 hand 18 h.There was no significant difference between the POD activity and the control at other time points.
To investigate the biological activity and action mechanism of Bacillus thuringiensis(Bt)on aphids,a strain of Bacillus thuringiensis subsp.thuringiensis was used as the fermentation product.The sputum was treated with 5 different concentrations of bacterial liquid,and the corrected mortality rate reached 29.74%-78.88%at 72 h.The concentration was positively correlated with mortality.After treated with the fermentation broth of Suyunjin subspecies and fermentation broth,the activity of superoxide dismutase(SOD)in the sorghum was inhibited from12 hto 24 h,and the inhibition rates were 21.06%-38.11% and 4.69%%-36.79%,respectively,At 18 hand 24 h after treatment,the inhibition of SOD activity reached a significant level(p<0.05).The effects of the fermentation broth and filtrate treatment group on the activity of catalase(CAT)were all activated before 18 h,and the fermentation broth and filtrate treatment group had the highest activation rate of CAT at 18 hand 12 h,respectively.The CAT viability was 1.8890 and 2.9463 folds of the control,respectively.The filtrate treatment group activated the activity of POD in Aphis farinosa Gmelin and the activation rate was 14.78%-57.39%.The POD activity in the fermentation broth treatment group was significantly higher than that in the control at 6 hand 18 h.There was no significant difference between the POD activity and the control at other time points.
引文
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[15]方燕,乔格侠,张广学.蚜虫寄主植物与取食部位的多样性[J].动物分类学报,2006(01):31-39.
[16]马亚玲,刘长仲.蚜虫的生态学特性及其防治[J].草业科学,2014(03):519-525.
[17]张新芳.栾树蚜虫发生规律及防治新技术[J].现代农业科技,2018(23):148-151.
[18]谷彦冰,张永军,谢微,等.蚜虫报警信息素对不同药剂防治桃蚜增效作用研究[J].农业与技术,2018(23):28-31.
[19]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002(04):503-507.
[20]蒋志胜,尚稚珍,万树青,等.光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响[J].昆虫学报,2003(01).
[21]牛宇,薛皎亮,谢映平.油松毛虫感染白僵菌后超氧化物歧化酶和过氧化氢酶的变化[J].应用与环境生物学报,2005,11(02):182,186.
[2]Schnepf E,Crickmore N,Rie J V,et al..Bacillus thuringiensis and its pesticidal crystal proteins[J].Microbiol Mol Biol Rev,1998,62(03):775-806.
[3]李今煜,陈小旋,关雄.苏云金芽孢杆菌抗癌的研究进展[J].农业生物技术学报,2002(03):301-304.
[4]Gould F,Martinez-Ramirez A,Anderson A,et al..Broadspectrum resistance to Bacillus thuringiensis toxins in Heliothis virescens,Proceedings of the National Academy of Sciences of the United States of America,1992,89(17):7986-7990.
[5]张继红,王琛柱,钦俊德.苏云金芽孢杆菌δ-内毒素的杀虫机理及其增效途径[J].昆虫学报,1998(03):101-110.
[6]洪海林,曹春霞,饶辉福,等.苏云金杆菌环保剂型防治蔬菜鳞翅目害虫试验[J].湖北植保,2014(05):13-14.
[7]徐庆贤,张无敌,尹芳,等.苏云金杆菌的利用现状、发展及问题探讨[J].农业与技术,2003(05):62-66.
[8]Shah J V,Yadav R,Ingle S S.Engineered Cry1Ac-Cry9Aa hybrid Bacillus thuringiensis delta-endotoxin with improved insecticidal activity against Helicoverpa armigera[J].Archives of Microbiology,2017,199(07):1-7.
[9]张立猛,田盼盼,孙跃先,等.云南烟区小春作物蚜虫和蚜茧蜂种类研究[J].云南农业大学学报(自然科学),2018(03):567-571.
[10]张海英,黄叶梅,吴小莉,等.一株抗高粱蚜芽孢杆菌的分离与鉴定[J].宜宾学院学报,2017(06):111-114.
[11]武海斌,宫庆涛,陈珍珍,等.昆虫病原线虫和噻虫嗪混用对韭蛆的杀虫效果及其体内保护酶和解毒酶活性的影响[J].昆虫学报,2018(07):851-859.
[12]黄菁,乔传令.昆虫解毒酶解毒机理及其在农药污染治理中的应用[J].农业环境科学学报,2002(03):285-287.
[13]Thomas M,Jelen G A.Hybrid Bacillus thuringiensis delta-endotoxins with novel broad-spectrum insecticidal activity,Monsanto Company,2000.
[14]王志英,孙丽丽,张健,等.苏云金杆菌和白僵菌可湿性粉剂研制及杀虫毒力测定[J].北京林业大学学报,2014(03):34-41.
[15]方燕,乔格侠,张广学.蚜虫寄主植物与取食部位的多样性[J].动物分类学报,2006(01):31-39.
[16]马亚玲,刘长仲.蚜虫的生态学特性及其防治[J].草业科学,2014(03):519-525.
[17]张新芳.栾树蚜虫发生规律及防治新技术[J].现代农业科技,2018(23):148-151.
[18]谷彦冰,张永军,谢微,等.蚜虫报警信息素对不同药剂防治桃蚜增效作用研究[J].农业与技术,2018(23):28-31.
[19]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002(04):503-507.
[20]蒋志胜,尚稚珍,万树青,等.光活化杀虫剂α-三噻吩的电子自旋共振分析及其对库蚊保护酶系统活性的影响[J].昆虫学报,2003(01).
[21]牛宇,薛皎亮,谢映平.油松毛虫感染白僵菌后超氧化物歧化酶和过氧化氢酶的变化[J].应用与环境生物学报,2005,11(02):182,186.