石墨烯量子点在生物医学中的应用:进展与挑战
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摘要
与其他纳米材料相比,以石墨烯量子点为基础的复合纳米材料,具有许多独特的理化性质,因此,在多个应用研究领域特别是在生物医学方面得到了高度关注。本篇综述中,笔者主要着眼于石墨烯量子点在生物传感器、体内外成像以及疾病治疗等领域的研究进展,并讨论了石墨烯量子点尚未解决以及在生物医学应用中有争议的话题,展望了石墨烯量子点为基础的纳米材料在生物医学方面的应用前景。
Graphene quantum dots(GQDs)-based nanohybrid materials have gained great attention in multiple research applications,particularly in biomedical fields due to their unique physicochemical properties and outstanding biocompatibility compared to other nanomaterials.In this review,we focus on the most recent emerging developments including biosensing applications,in vivo and in vitro imaging,and therapy of serious diseases.These unresolved problematic and controversial issues in their biomedical applications.Simultaneously,appplication prospect of GQD-based nanomaterials in biomedical field has also been put forward.
Graphene quantum dots(GQDs)-based nanohybrid materials have gained great attention in multiple research applications,particularly in biomedical fields due to their unique physicochemical properties and outstanding biocompatibility compared to other nanomaterials.In this review,we focus on the most recent emerging developments including biosensing applications,in vivo and in vitro imaging,and therapy of serious diseases.These unresolved problematic and controversial issues in their biomedical applications.Simultaneously,appplication prospect of GQD-based nanomaterials in biomedical field has also been put forward.
引文
[1]ADEEL M,BILAL M,RASHEED T,et al.Graphene and graphene oxide:Functionalization and nano-bio-catalytic system for enzyme immobilization and biotechnological perspective[J].Int J Biol Macromol,2018,120(Pt B):1430-1440.
[2]KALLURI A,DEBNATH D,DHARMADHIKARI B,et al.Graphene quantum dots:synthesis and applications[J].Methods Enzymol,2018,609:335-354.
[3]ARVAND M,HEMMATI S.Analytical methodology for the electro-catalytic determination of estradiol and progesterone based on graphene quantum dots and poly(sulfosalicylic acid)co-modified electrode[J].Talanta,2017,174:243-255.
[4]Pan M,Gu Y,Yun Y,et al.Nanomaterials for Electrochemical Immunosensing[J].Sensors(Basel),2017,17(5).
[5]LAWAL A T.Synthesis and utilisation of graphene for fabrication of electrochemical sensors[J].Talanta,2015,131:424-443.
[6]CHEN F,GAO W,QIU X,et al.Graphene quantum dots in biomedical applications:Recent advances and future challenges[J].Front Lab Med,2017,1(4):192-199.
[7]LAWAL A T.Progress in utilisation of graphene for electrochemical biosensors[J].Biosens Bioelectron,2018,106:149-178.
[8]BALAMURUGAN M,SANTHARAMAN P,MADA-SAMY T,et al.Recent trends in electrochemical biosensors of superoxide dismutases[J].Biosens Bioelectron,2018,116:89-99.
[9]ZHU JIN-LI,TANG YAN-FENG,WANG GANG,et al.Green,rapid,and Universal preparation approach of graphene quantum Dots under ultraviolet irradiation[J].ACSAppl Mater Interfaces,2017,9(16):14470-14477.
[10]BHATNAGAR D,KUMAR V,KUMAR A,et al.Graphene quantum dots FRET based sensor for early detection of heart attack in human[J].Biosens Bioelectron,2016,79:495-499.
[11]BHATNAGAR D,KAUR I,KUMAR A.Ultrasensitive cardiac troponin I antibody based nanohybrid sensor for rapid detection of human heart attack[J].Int J Biol Macromol,2017,95:505-510.
[12]HUANG KE-JING,LI JING,WU YING-YING,et al.Amperometric immunobiosensor for alpha-fetoprotein using Au nanoparticles/chitosan/TiO2-graphene composite based platform[J].Bioelectrochemistry,2013,90:18-23.
[13]RASHEED P A,SANDHYARANI N.Carbon nanostructures as immobilization platform for DNA:A review on current progress in electrochemical DNA sensors[J].Biosens Bioelectron,2017,97:226-237.
[14]KHAKBAZ F,MAHANI M.Micro-RNA detection based on fluorescence resonance energy transfer of DNA-carbon quantum dots probes[J].Anal Biochem,2017,523:32-38.
[15]QIAN ZHAO-SHENG,SHAN X Y,CHAI LU-JING,et al.DNA nanosensor based on biocompatible graphene quantum dots and Carbon nanotubes[J].Biosens Bioelectron,2014,60:64-70.
[16]HU T,ZHANG L,WEN W,et al.Enzyme catalytic amplification of miRNA-155detection with graphene quantum dot-based electrochemical biosensor[J].Biosens Bioelectron,2016,77:451-456.
[17]ROY E,PATRA S,TIWARI A,et al.Introduction of selectivity and specificity to graphene using an inimitable combination of molecular imprinting and nanotechnology[J].Biosens Bioelectron,2017,89(1,SI):234-248.
[18]LI K H,ZHAO X N,WEI G,et al.Recent advances in the cancer bioimaging with graphene quantum Dots[J].Curr Med Chem,2018,25(25):2876-2893.
[19]MIAO P,HAN K,TANG Y,et al.Recent advances in Carbon nanodots:synthesis,properties and biomedical applications[J].Nanoscale,2015,7(5):1586-1595.
[20]ARUMUGAM N,KIM J S.Quantum dots attached to graphene oxide for sensitive detection of ascorbic acid in aqueous solutions[J].Mater Sci Eng C Mater Biol Appl,2018,92:720-725.
[21]KUO W S,SHAO Y T,HUANG K S,et al.Antimicrobial Amino-Functionalized Nitrogen-Doped graphene quantum Dots for eliminating Multidrug-Resistant species in Dual-Modality photodynamic therapy and bioimaging under Two-Photon excitation[J].ACS Appl Mater Interfaces,2018,10(17):14438-14446.
[22]CHEN M L,HE Y J,CHEN X W,et al.Quantum-dotconjugated graphene as a probe for simultaneous cancertargeted fluorescent imaging,tracking,and monitoring drug delivery[J].Bioconjug Chem,2013,24(3):387-397.
[23]WANG H,CHEN Q W,ZHOU S Q.Carbon-based hybrid nanogels:a synergistic nanoplatform for combined biosensing,bioimaging,and responsive drug delivery[J].Chem Soc Rev,2018,47(11):4198-4232.
[24]WANG X J,SUN X,LAO J,et al.Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery[J].Colloids Surf B Biointerfaces,2014,122:638-644.
[25]YANG Y QI,CHEN S Z,LIU L H,et al.Increasing cancer therapy efficiency through targeting and localized light activation[J].ACS Appl Mater Interfaces,2017,9(28):23400-23408.
[26]KHODADADEI F,SAFARIAN S,GHANBARI N.Methotrexate-loaded nitrogen-doped graphene quantum dots nanocarriers as an efficient anticancer drug delivery system[J].Mater Sci Eng C Mater Biol Appl,2017,79:280-285.
[27]LIU Y,BHATTARAI P,DAI Z,et al.Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer[J].Chem Soc Rev,2018.doi:10.1039/c8cs00618k.[Epub ahead of print].
[28]ISAKOVIC A M,DULOVIC M,MARKOVIC I A,et al.Autophagy suppression sensitizes glioma cells to IMP dehydrogenase inhibition-induced apoptotic death[J].Exp Cell Res,2017,350(1):32-40.
[29]ZOU X F,ZHANG L,WANG Z J,et al.Mechanisms of the antimicrobial activities of graphene materials[J].JAm Chem Soc,2016,138(7):2064-2077.
[30]罗阳,杨永军.石墨烯材料抗菌活性机制研究--终结抗生素滥用的曙光[J].第三军医大学学报,2016(10):1047-1053.
[31]胡超.石墨烯量子点-银纳米材料的制备及其抗菌性能的研究[D].武汉:华中农业大学,2016.
[32]TABISH T A,SCOTTON C J,FERGUSON D C,et al.Biocompatibility and toxicity of graphene quantum dots for potential application in photodynamic therapy[J].Nanomedicine,2018,13(15):1923-1937.
[33]NURUNNABI M,KHATUN Z,HUH K M,et al.In vivo biodistribution and toxicology of carboxylated graphene quantum dots[J].ACS Nano,2013,7(8):6858-6867.
[34]PENG C,HU W B,ZHOU Y T,et al.Intracellular imaging with a Graphene-Based fluorescent probe[J].Small,2010,6(15):1686-1692.
[35]LI J L,BAO H C,HOU X L,et al.Graphene oxide nanoparticles as a nonbleaching optical probe for Two-Photon luminescence imaging and cell therapy[J].Angewandte Chemie-International Edition,2012,51(8):1830-1834.
[36]WANG D,ZHU L,CHEN J-F,et al.Can graphene quantum dots cause DNA damage in cells?[J].Nanoscale,2015,7(21):9894-9901.
[37]YUAN X C,LIU Z M,GUO Z Y,et al.Cellular distribution and cytotoxicity of graphene quantum dots with different functional groups[J].Nanoscale Res Lett,2014,9(1):108.
[38]MARKOVIC Z M,RISTIC B Z,ARSIKIN K M,et al.Graphene quantum dots as autophagy-inducing photodynamic agents[J].Biomaterials,2012,33(29):7084-7092.
[39]LUO C,LI Y F,GUO L J,et al.Graphene quantum Dots downregulate multiple Multidrug-Resistant genes via interacting with their C-Rich promoters[J].Adv Healthc Mater,2017,6(21):1-11.
[2]KALLURI A,DEBNATH D,DHARMADHIKARI B,et al.Graphene quantum dots:synthesis and applications[J].Methods Enzymol,2018,609:335-354.
[3]ARVAND M,HEMMATI S.Analytical methodology for the electro-catalytic determination of estradiol and progesterone based on graphene quantum dots and poly(sulfosalicylic acid)co-modified electrode[J].Talanta,2017,174:243-255.
[4]Pan M,Gu Y,Yun Y,et al.Nanomaterials for Electrochemical Immunosensing[J].Sensors(Basel),2017,17(5).
[5]LAWAL A T.Synthesis and utilisation of graphene for fabrication of electrochemical sensors[J].Talanta,2015,131:424-443.
[6]CHEN F,GAO W,QIU X,et al.Graphene quantum dots in biomedical applications:Recent advances and future challenges[J].Front Lab Med,2017,1(4):192-199.
[7]LAWAL A T.Progress in utilisation of graphene for electrochemical biosensors[J].Biosens Bioelectron,2018,106:149-178.
[8]BALAMURUGAN M,SANTHARAMAN P,MADA-SAMY T,et al.Recent trends in electrochemical biosensors of superoxide dismutases[J].Biosens Bioelectron,2018,116:89-99.
[9]ZHU JIN-LI,TANG YAN-FENG,WANG GANG,et al.Green,rapid,and Universal preparation approach of graphene quantum Dots under ultraviolet irradiation[J].ACSAppl Mater Interfaces,2017,9(16):14470-14477.
[10]BHATNAGAR D,KUMAR V,KUMAR A,et al.Graphene quantum dots FRET based sensor for early detection of heart attack in human[J].Biosens Bioelectron,2016,79:495-499.
[11]BHATNAGAR D,KAUR I,KUMAR A.Ultrasensitive cardiac troponin I antibody based nanohybrid sensor for rapid detection of human heart attack[J].Int J Biol Macromol,2017,95:505-510.
[12]HUANG KE-JING,LI JING,WU YING-YING,et al.Amperometric immunobiosensor for alpha-fetoprotein using Au nanoparticles/chitosan/TiO2-graphene composite based platform[J].Bioelectrochemistry,2013,90:18-23.
[13]RASHEED P A,SANDHYARANI N.Carbon nanostructures as immobilization platform for DNA:A review on current progress in electrochemical DNA sensors[J].Biosens Bioelectron,2017,97:226-237.
[14]KHAKBAZ F,MAHANI M.Micro-RNA detection based on fluorescence resonance energy transfer of DNA-carbon quantum dots probes[J].Anal Biochem,2017,523:32-38.
[15]QIAN ZHAO-SHENG,SHAN X Y,CHAI LU-JING,et al.DNA nanosensor based on biocompatible graphene quantum dots and Carbon nanotubes[J].Biosens Bioelectron,2014,60:64-70.
[16]HU T,ZHANG L,WEN W,et al.Enzyme catalytic amplification of miRNA-155detection with graphene quantum dot-based electrochemical biosensor[J].Biosens Bioelectron,2016,77:451-456.
[17]ROY E,PATRA S,TIWARI A,et al.Introduction of selectivity and specificity to graphene using an inimitable combination of molecular imprinting and nanotechnology[J].Biosens Bioelectron,2017,89(1,SI):234-248.
[18]LI K H,ZHAO X N,WEI G,et al.Recent advances in the cancer bioimaging with graphene quantum Dots[J].Curr Med Chem,2018,25(25):2876-2893.
[19]MIAO P,HAN K,TANG Y,et al.Recent advances in Carbon nanodots:synthesis,properties and biomedical applications[J].Nanoscale,2015,7(5):1586-1595.
[20]ARUMUGAM N,KIM J S.Quantum dots attached to graphene oxide for sensitive detection of ascorbic acid in aqueous solutions[J].Mater Sci Eng C Mater Biol Appl,2018,92:720-725.
[21]KUO W S,SHAO Y T,HUANG K S,et al.Antimicrobial Amino-Functionalized Nitrogen-Doped graphene quantum Dots for eliminating Multidrug-Resistant species in Dual-Modality photodynamic therapy and bioimaging under Two-Photon excitation[J].ACS Appl Mater Interfaces,2018,10(17):14438-14446.
[22]CHEN M L,HE Y J,CHEN X W,et al.Quantum-dotconjugated graphene as a probe for simultaneous cancertargeted fluorescent imaging,tracking,and monitoring drug delivery[J].Bioconjug Chem,2013,24(3):387-397.
[23]WANG H,CHEN Q W,ZHOU S Q.Carbon-based hybrid nanogels:a synergistic nanoplatform for combined biosensing,bioimaging,and responsive drug delivery[J].Chem Soc Rev,2018,47(11):4198-4232.
[24]WANG X J,SUN X,LAO J,et al.Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery[J].Colloids Surf B Biointerfaces,2014,122:638-644.
[25]YANG Y QI,CHEN S Z,LIU L H,et al.Increasing cancer therapy efficiency through targeting and localized light activation[J].ACS Appl Mater Interfaces,2017,9(28):23400-23408.
[26]KHODADADEI F,SAFARIAN S,GHANBARI N.Methotrexate-loaded nitrogen-doped graphene quantum dots nanocarriers as an efficient anticancer drug delivery system[J].Mater Sci Eng C Mater Biol Appl,2017,79:280-285.
[27]LIU Y,BHATTARAI P,DAI Z,et al.Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer[J].Chem Soc Rev,2018.doi:10.1039/c8cs00618k.[Epub ahead of print].
[28]ISAKOVIC A M,DULOVIC M,MARKOVIC I A,et al.Autophagy suppression sensitizes glioma cells to IMP dehydrogenase inhibition-induced apoptotic death[J].Exp Cell Res,2017,350(1):32-40.
[29]ZOU X F,ZHANG L,WANG Z J,et al.Mechanisms of the antimicrobial activities of graphene materials[J].JAm Chem Soc,2016,138(7):2064-2077.
[30]罗阳,杨永军.石墨烯材料抗菌活性机制研究--终结抗生素滥用的曙光[J].第三军医大学学报,2016(10):1047-1053.
[31]胡超.石墨烯量子点-银纳米材料的制备及其抗菌性能的研究[D].武汉:华中农业大学,2016.
[32]TABISH T A,SCOTTON C J,FERGUSON D C,et al.Biocompatibility and toxicity of graphene quantum dots for potential application in photodynamic therapy[J].Nanomedicine,2018,13(15):1923-1937.
[33]NURUNNABI M,KHATUN Z,HUH K M,et al.In vivo biodistribution and toxicology of carboxylated graphene quantum dots[J].ACS Nano,2013,7(8):6858-6867.
[34]PENG C,HU W B,ZHOU Y T,et al.Intracellular imaging with a Graphene-Based fluorescent probe[J].Small,2010,6(15):1686-1692.
[35]LI J L,BAO H C,HOU X L,et al.Graphene oxide nanoparticles as a nonbleaching optical probe for Two-Photon luminescence imaging and cell therapy[J].Angewandte Chemie-International Edition,2012,51(8):1830-1834.
[36]WANG D,ZHU L,CHEN J-F,et al.Can graphene quantum dots cause DNA damage in cells?[J].Nanoscale,2015,7(21):9894-9901.
[37]YUAN X C,LIU Z M,GUO Z Y,et al.Cellular distribution and cytotoxicity of graphene quantum dots with different functional groups[J].Nanoscale Res Lett,2014,9(1):108.
[38]MARKOVIC Z M,RISTIC B Z,ARSIKIN K M,et al.Graphene quantum dots as autophagy-inducing photodynamic agents[J].Biomaterials,2012,33(29):7084-7092.
[39]LUO C,LI Y F,GUO L J,et al.Graphene quantum Dots downregulate multiple Multidrug-Resistant genes via interacting with their C-Rich promoters[J].Adv Healthc Mater,2017,6(21):1-11.