ABCG2和ALDH1在卵巢子宫内膜异位症恶变中的表达和影响研究
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摘要
目的探讨三磷酸腺苷结合盒超家族G家族成员2(ABCG2)、乙醛脱氢酶1(ALDH1)在卵巢子宫内膜异位症恶变中的表达和意义。方法采用免疫组织化学法检测38例卵巢子宫内膜异位症恶变患者(EMs恶变组),35例卵巢子宫内膜异位囊肿患者(EMs组),30例正常子宫内膜患者(对照组)中ABCG2、ALDH1蛋白的表达。结果 ABCG2、ALDH1在EMs恶变组中的阳性表达率明显高于EMs组及对照组,比较差异有统计学意义(P<0.05)。EMs恶变组中ABCG2、ALDH1的表达与患者年龄、痛经史及病理学类型无明显相关性,与血清CA125水平、病理分化程度及分期呈明显相关性(P<0.05);ABCG2的表达与ALDH1的表达无明显相关性(P>0.05)。结论 ABCG2、ALDH1可能与卵巢子宫内膜异位症恶变的发生、发展有关。
Objective To investigate the expression and significance of ABCG2 and ALDH1in malignant transformation of ovarian endometriosis.Methods The protein expressions of ABCG2 and ALDH1were detected with immunohistochemistry in 38 cases of malignant transformation of ovarian endometriosis(EMs malignant transformation group),35 cases of endometriosis(EMs group)and 30 cases of normal endometrium(control group).Results The positive rates of ABCG2 and ALDH1expressions in EMs malignant transformation group were significantly higher than those in EMs group and control group(P<0.05).The expressions of ABCG2 and ALDH1were significantly associated with the serum CA125 level,differentiation and clinical stage of malignant transformation of ovarian endometriosis(P<0.05),but were not related to the patient′s age,dysmenorrhea history and pathology type(P>0.05).There was no correlation between the expression of ABCG2 and ALDH1in malignant transformation of ovarian endometriosis(P>0.05).Conclusion The expressions of ABCG2 and ALDH1might be involved in malignant transformation of ovarian endometriosis.
Objective To investigate the expression and significance of ABCG2 and ALDH1in malignant transformation of ovarian endometriosis.Methods The protein expressions of ABCG2 and ALDH1were detected with immunohistochemistry in 38 cases of malignant transformation of ovarian endometriosis(EMs malignant transformation group),35 cases of endometriosis(EMs group)and 30 cases of normal endometrium(control group).Results The positive rates of ABCG2 and ALDH1expressions in EMs malignant transformation group were significantly higher than those in EMs group and control group(P<0.05).The expressions of ABCG2 and ALDH1were significantly associated with the serum CA125 level,differentiation and clinical stage of malignant transformation of ovarian endometriosis(P<0.05),but were not related to the patient′s age,dysmenorrhea history and pathology type(P>0.05).There was no correlation between the expression of ABCG2 and ALDH1in malignant transformation of ovarian endometriosis(P>0.05).Conclusion The expressions of ABCG2 and ALDH1might be involved in malignant transformation of ovarian endometriosis.
引文
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[12]张春妤,郭红燕,韩劲松,等.三磷酸腺苷结合盒转运蛋白G2、F2在卵巢上皮性癌组织中的表达与意义[J].中华妇产科杂志,2014,49(3):224-225.
[13]Zhang QH,Dou HT,Xu P,et al.Tumor recurrence and drug resistance properties of side population cells in high grade ovary cancer[J].Drug Res(Stuttg),2015,65(3):153-157.
[14]于慧玲,李冬梅,吴罡,等.子宫内膜癌组织中ALDH1、p53和CyclinE蛋白的表达及意义[J].中国妇幼保健,2014,29(25):4165-4167.
[15]范秀丽,卞翠翠,董怡,等.ALDH1作为卵巢癌干细胞标志物的实验研究[J].中国老年学杂志,2015,35(12):3266-3268.
[2]Nozawa-Suzuki N,Nagasawa H,Ohnishi K,et al.The inhibitory effect of hypoxic cytotoxin on the expansion of cancer stem cells in ovarian cancer[J].Biochem Biophys Res Commun,2015,457(4):706-711.
[3]Yan HC,Fang LS,Xu J,et al.The identification of the biological characteristics of human ovarian cancer stem cells[J].Eur Rev Med Pharmacol Sci,2014,18(22):3497-3503.
[4]刘颂平,温坚,殷新明.子宫内膜异位症患者腹腔冲洗液及血清CD133、ABCG2的表达[J].中国医药导报,2013,10(24):45-47.
[5]温坚,刘颂平,殷新明.乙醛脱氢酶1与子宫内膜异位症的相关性研究[J].中国妇幼保健,2013,28(35):5878-5881.
[6]Guo SW.Endometriosis and ovarian cancer:potential benefits and harms of screening and risk-reducing surgery[J].Fertil Steril,2015,104(4):813-830.
[7]Scott RB.Malignant changes in endometriosis[J].Obstet Gynecol,1953,2(3):283-289.
[8]吕昕,王悦红,周建英.TTF-1,SPA表达与Ⅱ期肺腺癌患者术后复发及生存关系的研究[J].浙江医学,2015,37(2):117-123.
[9]Szepesi A,Matula Z,Szigeti A,et al.ABCG2is a selectable marker for enhanced multilineage differentiation potential in periodontal ligament stem cells[J].Stem Cells Dev,2015,24(2):244-252.
[10]Kim N,Choung HK,Lee MJ,et al.Cancer stem cell markers in eyelid sebaceous gland carcinoma:high expression of ALDH1,CD133,and ABCG2correlates with poor prognosis[J].Invest Ophthalmol Vis Sci,2015,56(3):1813-1819.
[11]王任晓.宫颈癌组织中ABCG2与CD133的表达对预后的影响分析[J].实用癌症杂志,2014,29(6):614-616.
[12]张春妤,郭红燕,韩劲松,等.三磷酸腺苷结合盒转运蛋白G2、F2在卵巢上皮性癌组织中的表达与意义[J].中华妇产科杂志,2014,49(3):224-225.
[13]Zhang QH,Dou HT,Xu P,et al.Tumor recurrence and drug resistance properties of side population cells in high grade ovary cancer[J].Drug Res(Stuttg),2015,65(3):153-157.
[14]于慧玲,李冬梅,吴罡,等.子宫内膜癌组织中ALDH1、p53和CyclinE蛋白的表达及意义[J].中国妇幼保健,2014,29(25):4165-4167.
[15]范秀丽,卞翠翠,董怡,等.ALDH1作为卵巢癌干细胞标志物的实验研究[J].中国老年学杂志,2015,35(12):3266-3268.