微流控技术在口岸呼吸道传染病检测中的应用
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摘要
目的呼吸道感染是全球关注的公共卫生问题,随着国际贸易的快速发展,在人流和物流集中的国境口岸传播风险极高。因此当务之急是口岸卫生检疫部门寻找一种多通量、检测速度快、特异性高和准确性好的呼吸道病原体检测技术。方法目前呼吸道病原体检测技术主要有微生物的分离培养、血清免疫学和核酸检测技术,但是存在检测时间长、通量小和不适合口岸现场检测等缺点,不能满足口岸现场检测要求。结果总结分析呼吸道病原体的检测技术,微流控技术应用在口岸呼吸道传染病检测具有很强的实用性。结论微流控技术具有通量大、便携性和准确性好等优点,可以满足口岸呼吸道传染病检测的方法要求。
Objective Respiratory tract infections(RTIs) are a public health issue of global concern. With the rapid development of international trade,the risk of transmission is extremely high at frontier ports where people flow and logistics beingconcentrated. Therefore,it is urgent for the port health and quarantine adminstrationto find a multi-flux,rapiddetection,high specificity and accuracy of respiratory pathogens detection technology. Methods Currently,the detection technologies of respiratory pathogens mainly include microorganism isolation and culture,serum immunology and nucleic acid detection,etc.. However,these technologies have some disadvantages such as long detection time,small flux and unsuitability for port field detection,,which cannot meet the requirements of port field detection. Results It is concluded that the application of microfluidic control technology in the detection of respiratory infectious diseases at ports is very practical. Conclusion Microfluidic control technology has the advantages of large flux,portability and accuracy,and can meet the requirements of the method for the detection of respiratory infectious diseases at ports.
Objective Respiratory tract infections(RTIs) are a public health issue of global concern. With the rapid development of international trade,the risk of transmission is extremely high at frontier ports where people flow and logistics beingconcentrated. Therefore,it is urgent for the port health and quarantine adminstrationto find a multi-flux,rapiddetection,high specificity and accuracy of respiratory pathogens detection technology. Methods Currently,the detection technologies of respiratory pathogens mainly include microorganism isolation and culture,serum immunology and nucleic acid detection,etc.. However,these technologies have some disadvantages such as long detection time,small flux and unsuitability for port field detection,,which cannot meet the requirements of port field detection. Results It is concluded that the application of microfluidic control technology in the detection of respiratory infectious diseases at ports is very practical. Conclusion Microfluidic control technology has the advantages of large flux,portability and accuracy,and can meet the requirements of the method for the detection of respiratory infectious diseases at ports.
引文
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[2]马文.多重快速检测6种呼吸道病毒的研究[D].军事科学院,2018.
[3]郝良玉,曲晗,李志萍,等.微流控技术在病原微生物检测中的应用[J].检验医学与临床,2018,15(21):3299-3302.
[4] Wan L, Chen T, Gao J, et al. A digital microfluidicsys-tem for loop-mediated isothermal amplification andse-quenec specific pathogen detection[J]. Sci Rep,2017,7:14586.
[5] Burns MA,JohnsonBN,BrahmasandraSN,etal.An integratednanoliter DNA analysis device[J]. Science,1998,282(5388):484-487.
[6] Manz A, Graber N, Widmer H M. Miniaturized total chemi-cal analysis systems:A novel concept for chemical sens-ing[J]. Sensor Actuartos B,1990,1(1):244-248.
[7] Sui G D, Wang J Y, Lee C C, et al.Solution-phase sur-face modification in intact poly microfluidic channels[J].Ana-lytical Chemistry,2006,78(15):5543-5551.
[8] Andersson H. Microtechnologies and nanotechnologies forsingle-cell analysis[J].Current Opinion Biotechnolo-gy,2004,15(1):44-49.
[9]毕颖楠,张惠静.微流控分析芯片在医学领域的应用[J].生物工程学报,2006,22(1):167-171.
[10]孔文.几种呼吸道传染病病毒的微流控等温扩增荧光检测技术研究[D].广西医科大学,2018.
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[13] FERNANDES A C, SEMENOVA D, PANJAN P, et al.Multi-function microfluidic platform for sensor integra-tion[J]. New Biotechnol,2018,162(1):2231-2237.