北虫草中虫草素和虫草多糖的提取纯化研究
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摘要
目的研究北虫草中虫草素和虫草多糖的提取纯化工艺。方法采用正交实验法研究提取工艺,采用柱分离法,以虫草素和虫草多糖的含量为指标,考察流速、上样体积和洗脱剂浓度对纯化工艺的影响。结果提取的最佳工艺为:加10倍量水,回流提取3次,每次1h。优化的纯化工艺为:将提取液浓缩至1mL,相当于北虫草粉0.1g,以2BV·h~(-1)的流速上样,上样体积为0.6BV,先用2BV的水洗脱,再用4BV体积分数为40%的乙醇溶液以1～2BV·h~(-1)的流速洗脱,收集乙醇洗脱液。结论该提取纯化工艺经验证实验证明可行。
Objective To study the purification process of cordycepin and cordyceps polysaccharide in Cordyceps militaris.Methods Orthogonal experiment was used to study the extraction process.Column separation method was used to investigate the effect of the flow rate,the volume of the upper sample and the concentration of the eluent on the purification process with the evaluation index of the content of cordyceps and cordyceps polysaccharide.Results The optimal extraction process was as follows:adding 10 times of water,refluxing for 3 times with 1 heach time.The optimized purification process was as follows:the extraction solution was concentrated to 1 mL,which was equivalent to 0.1 g of Cordyceps militaris.The volume of the sample was 0.6 BV with the flow rate of 2 BV·h~(-1),firstly washed with 2 BV of water,followed by 4 BV of 40% ethanol solution with the flow rate of 1-2 BV·h~(-1).Finally,the ethanol eluent was collected.Conclusion The extraction and purification process was proved to be feasible.
Objective To study the purification process of cordycepin and cordyceps polysaccharide in Cordyceps militaris.Methods Orthogonal experiment was used to study the extraction process.Column separation method was used to investigate the effect of the flow rate,the volume of the upper sample and the concentration of the eluent on the purification process with the evaluation index of the content of cordyceps and cordyceps polysaccharide.Results The optimal extraction process was as follows:adding 10 times of water,refluxing for 3 times with 1 heach time.The optimized purification process was as follows:the extraction solution was concentrated to 1 mL,which was equivalent to 0.1 g of Cordyceps militaris.The volume of the sample was 0.6 BV with the flow rate of 2 BV·h~(-1),firstly washed with 2 BV of water,followed by 4 BV of 40% ethanol solution with the flow rate of 1-2 BV·h~(-1).Finally,the ethanol eluent was collected.Conclusion The extraction and purification process was proved to be feasible.
引文
[1]蒋志涛,戴国梁,潘金火,等.蛹虫草化学成分及药理作用研究进展[J].现代中药研究与实践,2015,29(5):80-83.
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[3]陈亚丽,洪妍,李继平,等.HPLC法测定冬虫夏草中核苷类成分的含量[J].西北药学杂志,2017,32(4):403-406.
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[8]孙娜,崔建美,李继安,等.冬虫夏草的化学成分与药理作用分析研究[J].辽宁中医药大学学报,2015,17(4):65-67.
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[12]钟石,李有贵,陈诗,等.人工培养蛹虫草与冬虫夏草的主要活性成分比较[J].蚕业科学,2009,35(4):831-836.
[13]李刚,朱华李,毛先兵,等.蛹虫草中虫草素分离纯化工艺研究进展[J].重庆中草药研究,2006,(1):51-54,29.
[14]陈星.一种虫草素的纯化方法:中国,00134688.1[P].2001-06-13.
[15]陈顺志,高剑萍.一种超临萃取虫草脱氧核苷的生产方法:中国:01126910.3[P].2002-03-13.
[16]贺君,王云慧,杜昊.一种虫草多糖的提取工艺:中国,200410023662.7[P].2005-01-05.
[17]刘春泉,宋江峰,李大婧.从蛹虫草中提取精制虫草素和虫草多糖的方法:中国,200710122409.0[P].2008-02-20.
[18]李如意,宋厚盼,魏艳霞,等.冬虫夏草药理作用的研究进展[J].环球中医药,2016,9(10):1284-1288.
[19]严冬,梁举春.冬虫夏草药理作用研究综述[J].北方药学,2013,10(4):54.
[20]刘源才,王磊,陈默思.冬虫夏草药理研究进展[J].食品与药品,2015,17(5):374-377.
[2]陈俐彤,曹红峰,黄文芳.蛹虫草的化学成分、药效及应用[J].现代食品科技,2005,21(3):192-194,197.
[3]陈亚丽,洪妍,李继平,等.HPLC法测定冬虫夏草中核苷类成分的含量[J].西北药学杂志,2017,32(4):403-406.
[4]吴仪洛.本草从新[M].天津:天津科学技术出版社,2003:25-27.
[5]郑壮丽,黄春花,梅彩英,等.蛹虫草国内外研究的新进展[J].环境昆虫学报,2011,33(2):225-233.
[6]孟泽彬,陈林会,韩近雨,等.蛹虫草化学活性成分的研究进展[J].分子植物育种,2015,13(9):2147-2154.
[7]喻林红,林伟.冬虫夏草的化学成分和药理作用及临床应用[J].海峡药学,2013,25(12):63-66.
[8]孙娜,崔建美,李继安,等.冬虫夏草的化学成分与药理作用分析研究[J].辽宁中医药大学学报,2015,17(4):65-67.
[9]万朋,高俊涛,吕世杰.蛹虫草化学成分及药理作用研究进展[J].上海中医药杂志,2015,49(6):95-97.
[10]邹赢锌,陈雅琳,储智勇,等.冬虫夏草成分及活性研究进展[J].海军医学杂志,2014,35(1):83-85.
[11]张志红,杨璐敏,邰丽梅,等.冬虫夏草生理活性成分的研究进展[J].中国食用菌,2014,33(4):1-4.
[12]钟石,李有贵,陈诗,等.人工培养蛹虫草与冬虫夏草的主要活性成分比较[J].蚕业科学,2009,35(4):831-836.
[13]李刚,朱华李,毛先兵,等.蛹虫草中虫草素分离纯化工艺研究进展[J].重庆中草药研究,2006,(1):51-54,29.
[14]陈星.一种虫草素的纯化方法:中国,00134688.1[P].2001-06-13.
[15]陈顺志,高剑萍.一种超临萃取虫草脱氧核苷的生产方法:中国:01126910.3[P].2002-03-13.
[16]贺君,王云慧,杜昊.一种虫草多糖的提取工艺:中国,200410023662.7[P].2005-01-05.
[17]刘春泉,宋江峰,李大婧.从蛹虫草中提取精制虫草素和虫草多糖的方法:中国,200710122409.0[P].2008-02-20.
[18]李如意,宋厚盼,魏艳霞,等.冬虫夏草药理作用的研究进展[J].环球中医药,2016,9(10):1284-1288.
[19]严冬,梁举春.冬虫夏草药理作用研究综述[J].北方药学,2013,10(4):54.
[20]刘源才,王磊,陈默思.冬虫夏草药理研究进展[J].食品与药品,2015,17(5):374-377.