海参酶解液对角叉菜胶所致小鼠血栓形成的抑制作用
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摘要
【目的】探讨海参酶解液对角叉菜胶所致的小鼠尾部血栓模型的改善作用。【方法】将实验小鼠分对照组、模型组、以及低、中、高(350、700、1 400 mg/kg)剂量组,采用海参酶解液和生理盐水连续30 d灌胃处理,以角叉菜胶制备小鼠尾部血栓模型,计算24 h和48 h时黑尾长度占尾部总长度的百分比即黑尾比率(%),采用酶联免疫吸附法(ELISA)测定血浆纤维蛋白原(FIB)、纤维蛋白原降解产物(FDP)和组织型纤溶酶原激活剂(t-PA)的含量。【结果】各组小鼠灌胃海参酶解液前后与对照组比体质量无显著性差异(P>0.05);海参酶解液中剂量组与高剂量组小鼠的黑尾比率明显小于模型组(P<0.05),海参酶解液低剂量组小鼠黑尾比率与模型组相比无显著性差异;灌胃海参酶解液组小鼠血浆中的FIB含量显著低于模型组(P<0.05);高剂量组与中剂量组小鼠血浆中的FDP及t-PA的含量显著高于模型组(P<0.05)。【结论】海参酶解液对模型小鼠体内血栓的形成具有改善和溶解作用。
【Objective】To investigate the protective effect of sea cucumber enzymatic hydrolysate(SCEH) on carrageenan-induced tail thrombosis in mice, and provide theoretical basis for the evaluation of thrombolytic function on edible sea cucumber health products.【Method】Mice were divided into Control groups, model groups, and SCEH-treated groups at 350, 700, 1 400 mg/kg. After continuously administrating saline or SCEH for 4 weeks, all animals, except for the control group, were intravenously injected with carrageenan to set up the tail thrombosis model. Then black tail ratio(the percentage of black tail length to total tail length) at 24 h and 48 h(%) were calculated and the levels of fibrinogen(FIB), fibrinogen degradation product(FDP) and tissue plasminogen activator(t-PA) were measured by ELISA. 【 Result 】 There was no difference in body weight among the groups before and after administration of SCEH. The black tail ratios in SCEH-treated groups at 700 and 1400 mg/kg were significantly shorter than that of the model group(P < 0.05). There was no significant difference in the black tail ratio between low dose of SCEH and the model group. The plasma FIB contents in all SCEH-treated mice were significantly lower than that in the model group(P < 0.05), while the plasma levels of FDP and t-PA in SCEH-treated groups at 700 and 1400 mg/kg were significantly higher than those in the model group(P < 0.05).【Conclusion】The SCEH can inhibit the thrombus formation induced by carrageenan in mice.
【Objective】To investigate the protective effect of sea cucumber enzymatic hydrolysate(SCEH) on carrageenan-induced tail thrombosis in mice, and provide theoretical basis for the evaluation of thrombolytic function on edible sea cucumber health products.【Method】Mice were divided into Control groups, model groups, and SCEH-treated groups at 350, 700, 1 400 mg/kg. After continuously administrating saline or SCEH for 4 weeks, all animals, except for the control group, were intravenously injected with carrageenan to set up the tail thrombosis model. Then black tail ratio(the percentage of black tail length to total tail length) at 24 h and 48 h(%) were calculated and the levels of fibrinogen(FIB), fibrinogen degradation product(FDP) and tissue plasminogen activator(t-PA) were measured by ELISA. 【 Result 】 There was no difference in body weight among the groups before and after administration of SCEH. The black tail ratios in SCEH-treated groups at 700 and 1400 mg/kg were significantly shorter than that of the model group(P < 0.05). There was no significant difference in the black tail ratio between low dose of SCEH and the model group. The plasma FIB contents in all SCEH-treated mice were significantly lower than that in the model group(P < 0.05), while the plasma levels of FDP and t-PA in SCEH-treated groups at 700 and 1400 mg/kg were significantly higher than those in the model group(P < 0.05).【Conclusion】The SCEH can inhibit the thrombus formation induced by carrageenan in mice.
引文
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[33]王磊磊.现代色谱分析技术对海参脂肪酸成分的研究[D].上海:上海海洋大学,2009.
[2]龚丽英,袁乐宏,黄洁,等.口服抗栓药物的研究进展[J].中国临床药理学杂志,2016,32(3):279-282.
[3]丁家欣,徐琦,欧兴长,等.重组水蛭素的研究进展[J].中国药学杂志,1994,29(3):134-138.
[4]黄维义,李刚,严丽,等.华法林在慢性心房颤动抗凝治疗中的应用[J].临床心血管病杂志,2006,22(5):276-278.
[5]廖上腾,杨静,刘颖,等.新型ADP受体拮抗剂的合成及其抗凝血活性[J].合成化学,2014,22(2):196-199.
[6]韦豪华,张红玲,李兴太.海参化学成分及生物活性研究进展[J].食品安全质量检测学报,2017,8(6):2054-2061.
[7]FU X Y,XUE C H,MIAO B C,et al.Characterization of proteases from the digestive tract of sea cucumber(Stichopus japonicus):High alkaline protease activity[J].Aquaculture,2005,246(1/2/3/4):321-329.
[8]ZHOU Y,YANG H,LIU S,et al.Feeding and growth on bivalve biodeposits by the deposit feeder Stichopus japonicus Selenka(Echinodermata:Holothuroidea)co-cultured in lantern nets[J].Aquaculture,2006,256(1/2/3/4):510-520.
[9]KALININ VI.System-theoretical(holistic)approach to the modelling of structural–functional relationships of biomolecules and their evolution:an example of triterpene glycosides from sea cucumbers(Echinodermata,Holothurioidea)[J].Journal of Theoretical Biology,2000,206(1):151-168.
[10]TROTTER J A,LYONS-LEVY G,THURMOND F A,et al.Covalent composition of collagen fibrils from the dermis of the sea cucumber,Cucumaria frondosa,a tissue with mutable mechanical properties[J].Comparative Biochemistry and Physiology Part A Physiology,1995,112(3/4):463-478.
[11]LI Z,WANG H,LI J.Basic and clinical study on the antithrombotic mechanism of glycosaminoglycan extracted from sea cucumber[J].Chinese Medical Journal,2000,113(8):706-711.
[12]王静,张京楼,王铎喜,等.海参多肽的抗氧化性能研究[J].食品与机械,2010,26(2):67-71.
[13]王林.海参功能保健黄酒的研发[D].青岛:中国海洋大学,2012.
[14]赵兴坤.海参肽的功能特性及其应用[J].中国食物与营养,2003(12):31-33.
[15]逄龙.海参主要活性物质对血管内皮细胞的保护作用及抗肿瘤活性的研究[D].青岛:中国海洋大学,2007.
[16]WU M,XU S,ZHAO J,et al.Free-radical depolymerization of glycosaminoglycan from sea cucumber Thelenata ananas by hydrogen peroxide and copper ions[J].Carbohydrate Polymers,2010,80(4):1116-1124.
[17]王奇,李妍妍,芦红艳,等.东海海参酶解液提高小鼠记忆力和抗疲劳功能的研究[J].营养学报,2011,33(6):580-583;588.
[18]刘彦霞,陈文,温宗妍,等.纳豆激酶对角叉菜胶所致小鼠血栓模型的影响[J].食品工业科技,2012,33(20):330-332;336.
[19]MA N,LIU X W,YANG Y J,et al.Preventive Effect of Aspirin Eugenol Ester on Thrombosis inκ-Carrageenan-Induced Rat Tail Thrombosis Model[J].Plos One,2015,10(7):e0133125.
[20]刘彦霞,郭豫,赵江燕,等.角叉菜胶致血栓动物模型制备及在功能食品评价中的应用[J],食品工业.2012,33(8):93-96.
[21]谢德芳,方成,王春维,等.蚓激酶抗角叉菜胶诱导大鼠血栓形成作用[J].中国医药导刊,2013,15(11):1877-1878;1880.
[22]KAMIYA S,OGASAWARA M,ARAKAWA M,et al.The Effect of Lactic Acid Bacteria-fermented Soybean Milk Products on Carrageenan-induced Tail Thrombosis in Rats[J].Bioscience of Microbiota Food&Health,2013,32(3):101-105.
[23]么秀洁,赵志梅,夏天.炎症与血栓形成[J].血栓与止血学,2015,21(3):190-192.
[24]张卉,贺蓉,贺石林.炎症与血栓形成[J].中华医学杂志,2004,84(9):784-786.
[25]杨欢,程笑,王月华,等.秦皮甲素对角叉菜胶致小鼠足肿胀及炎症的作用[J],中国新药杂志.2016,25(20):2319-2322.
[26]WANG G Q,XU T,BU X M,et al.Anti-inflammation effects of corn silk in a rat model of carrageenin-induced pleurisy[J].Inflammation,2012,35(3):822-827.
[27]魏陵博,戎冬梅,吉中强,等.角叉菜胶致大鼠尾部血栓形成的机制[J].中西医结合心脑血管病杂志,2008,6(5):542-543.
[28]孙瑞,朱武生.缺血性卒中溶栓后出血转化发生机制的研究进展[J].中国脑血管病杂志,2018,15(1):52-56.
[29]杨娟.延胡索乙素抗血栓作用及机制研究[D].郑州:郑州大学,2013.
[30]王学锋,王鸿利,胡大萌,等.玉足海参酸性粘多糖(抗栓胶囊)抗血栓形成作用的观察[J].中华血液学杂志,1997,18(9):457-459.
[31]王学锋,李志文,储海燕,等.海参糖胺聚糖抗血栓形成机制的研究[J].中国新药与临床杂志,2002,21(12):718-721.
[32]沈卫章,周荣富,王学锋,等.玉足海参糖胺聚糖抗血栓形成的研究[J].中华血液学杂志,2006,27(9):579-583.
[33]王磊磊.现代色谱分析技术对海参脂肪酸成分的研究[D].上海:上海海洋大学,2009.