纳米银涂层可吸收线吻合小鼠肠壁近期抗炎疗效的实验研究
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摘要
目的探讨纳米银涂层可吸收缝合线的近期抗炎疗效。方法采用化学涂层法将纳米银颗粒涂层在普通可吸收缝合线(Vicryl suture,4-0,Polyglactin 910)表面,制备纳米银涂层线。12只Balb/C小鼠随机分3组:普通可吸收线、抗菌可吸收线和纳米银涂层可吸收线。在小鼠回肠制备肠切开模型后,分别采用上述3组缝线行单层间断端端吻合。术后7天取材吻合口处肠管组织标本,连续冰冻切片进行白介素6(IL-6)、肿瘤坏死因子α(TNF-α)、增殖性细胞核抗原(proliferative cell nuclear antigen,PCNA)免疫组化观察和半定量分析。结果免疫组化和半定量分析显示纳米银涂层可吸收线组吻合口处IL-6和TNF-α表达最少,普通可吸收线组IL-6和TNF-α表达最多(普通、抗菌和纳米银涂层可吸收线IL-6的光密度值分别为0.25±0.03、0.19±0.02、0.10±0.02,F=41.378,P=0.000;TNF-α光密度值分别为0.39±0.04,0.29±0.03,0.13±0.02,F=77.885,P=0.000)。PCNA标志物染色显示纳米银涂层线组吻合口内增殖细胞数量最多[(48.0±7.6)个,(83.5±14.2)个,(126.5±13.2)个,F=42.857,P=0.000]。结论纳米银涂层可吸收线用于肠切除肠吻合之后,吻合口处炎症反应轻微,组织细胞增生和修复明显。
Objective To explore the anti-inflammatory efficacy of Vicryl suture( Polyglactin 910) coated with silver nanoparticles. Methods The absorbable suture( 4-0 in size) was coated with silver nanoparticles through layer-by-layer deposition.A total of 12 Balb/C mice were randomly divided into 3 groups: normal suture group,antibacterial suture group and silver nanoparticles coated suture group. After the mouse ileum was prepared for intestine incision model,the above three groups of sutures were then respectively utilized for single layer interrupted anastomosis of ileum. The tissue samples at post-anastomosis day 7 were harvested to investigate IL-6,TNF-α and proliferative cell nuclear antigen( PCNA) expression via IHC staining and semi-quantitative analysis.Results The immunohistochemistry and semi-quantitative analysis results showed that IL-6 and TNF-α production was at the least levels around anastomotic site in the silver nanoparticles coated suture group,while the most in the normal suture group [the optical delnsity( OD) values of IL-6 in the normal suture,antibacterial suture and silver nanoparticles coated suture group were 0. 25 ± 0. 03,0. 19 ± 0. 02,and 0. 10 ± 0. 02,respectively,F = 41. 378,P = 0. 000; The OD values of TNF-α in the normal suture,antibacterial suture and silver nanoparticles coated suture group were 0. 39 ± 0. 04,0. 29 ± 0. 03,and 0. 13 ± 0. 02,respectively,F = 77. 885,P =0. 000]. The highest PCNA expression was found in the silver nanoparticles coated suture group [the proliferating cells numbers under high magnification random field of vision around anastomotic tissue in the normal suture,antibacterial suture and silver nanoparticles coated suture group were( 48. 0 ± 7. 6) cells,( 83. 5 ± 14. 2) cells,and( 126. 5 ± 13. 2) cells,respectively,F = 42. 857,P =0. 000]. Conclusion Silver nanoparticles-coated sutures have potential to inhibit local inflammation and promote cell proliferation,as well as contribute to intestinal post-anastomotic healing.
Objective To explore the anti-inflammatory efficacy of Vicryl suture( Polyglactin 910) coated with silver nanoparticles. Methods The absorbable suture( 4-0 in size) was coated with silver nanoparticles through layer-by-layer deposition.A total of 12 Balb/C mice were randomly divided into 3 groups: normal suture group,antibacterial suture group and silver nanoparticles coated suture group. After the mouse ileum was prepared for intestine incision model,the above three groups of sutures were then respectively utilized for single layer interrupted anastomosis of ileum. The tissue samples at post-anastomosis day 7 were harvested to investigate IL-6,TNF-α and proliferative cell nuclear antigen( PCNA) expression via IHC staining and semi-quantitative analysis.Results The immunohistochemistry and semi-quantitative analysis results showed that IL-6 and TNF-α production was at the least levels around anastomotic site in the silver nanoparticles coated suture group,while the most in the normal suture group [the optical delnsity( OD) values of IL-6 in the normal suture,antibacterial suture and silver nanoparticles coated suture group were 0. 25 ± 0. 03,0. 19 ± 0. 02,and 0. 10 ± 0. 02,respectively,F = 41. 378,P = 0. 000; The OD values of TNF-α in the normal suture,antibacterial suture and silver nanoparticles coated suture group were 0. 39 ± 0. 04,0. 29 ± 0. 03,and 0. 13 ± 0. 02,respectively,F = 77. 885,P =0. 000]. The highest PCNA expression was found in the silver nanoparticles coated suture group [the proliferating cells numbers under high magnification random field of vision around anastomotic tissue in the normal suture,antibacterial suture and silver nanoparticles coated suture group were( 48. 0 ± 7. 6) cells,( 83. 5 ± 14. 2) cells,and( 126. 5 ± 13. 2) cells,respectively,F = 42. 857,P =0. 000]. Conclusion Silver nanoparticles-coated sutures have potential to inhibit local inflammation and promote cell proliferation,as well as contribute to intestinal post-anastomotic healing.
引文
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9 Liu X,Lee PY,Ho CM,et al.Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing.Chem Med Chem,2010,5(3):468-475.
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11 Perelshtein I,Applerot G,Perkas N,et al.Sonochemical coating of silver nanoparticles on textile fabrics(nylon,polyester and cotton)and their antibacterial activity.Nanotechnology,2008,19(24):245705.
12 Du Juan,Liu Xuelai.The application of nano biomaterials in diabetic wounds.中华内分泌外科杂志,2014,8(6):463-466.
2 Mashock MJ,Kappell AD,Hallaj N,et al.Copper oxide nanoparticles inhibit the metabolic activity of Saccharomyces cerevisiae.Environ Toxicol Chem,2016,35(1):134-143.
3 Harshiny M,Matheswaran M,Arthanareeswaran G,et al.Enhancement of antibacterial properties of silver nanoparticlesceftriaxone conjugate through Mukia maderaspatana leaf extract mediated synthesis.Ecotoxicol Environ Saf,2015,121:135-141.
4 Tian B,Chen W,Yu D,et al.Fabrication of silver nanoparticledoped hydroxyapatite coatings with oriented block arrays for enhancing bactericidal effect and osteoinductivity.J Mech Behav Biomed Mater,2016,61:345-359.
5 刘雪来,黄格元.纳米生物材料在创伤修复中的研究与应用.中华外科杂志,2013,51(8):748-752.
6 Tian J,Wong KK,Ho CM,et al.Topical delivery of silver nanoparticles promotes wound healing.Chem Med Chem,2007,2(1):129-136.
7 Wong KK,Cheung SO,Huang L,et al.Further evidence of the antiinflammatory effects of silver nanoparticles.Chem Med Chem,2009,4(7):1129-1135.
8 Liu X,Hao W,Lok CN,et al.Dendrimer encapsulation enhances anti-inflammatory efficacy of silver nanoparticles.J Pediatr Surg,2014,49(12):1846-1851.
9 Liu X,Lee PY,Ho CM,et al.Silver nanoparticles mediate differential responses in keratinocytes and fibroblasts during skin wound healing.Chem Med Chem,2010,5(3):468-475.
10 Kwan KH,Liu X,To MK,et al.Modulation of collagen alignment by silver nanoparticles results in better mechanical properties in wound healing.Nanomedicine,2011,7(4):497-504.
11 Perelshtein I,Applerot G,Perkas N,et al.Sonochemical coating of silver nanoparticles on textile fabrics(nylon,polyester and cotton)and their antibacterial activity.Nanotechnology,2008,19(24):245705.
12 Du Juan,Liu Xuelai.The application of nano biomaterials in diabetic wounds.中华内分泌外科杂志,2014,8(6):463-466.