同步酶解发酵瞬时弹射式蒸汽爆破玉米秸秆生产生物饲料研究
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摘要
通过优化瞬时弹射式蒸汽爆破(Instant Catapult Steam Explosion,ICSE)预处理玉米秸秆同步酶解发酵(Synchronous saccharification and fermentation,SSF)工艺,提高玉米秸秆转化生物饲料工艺的效率和可行性。使用合成培养基和秸秆酶解物培养产朊假丝酵母(Candida utilis BNCC 336517),比较不同温度条件下单纯酶解和同步酶解发酵对瞬时弹射式蒸汽爆破预处理秸秆的糖转化率影响;进一步设计正交试验获得玉米秸秆转化真蛋白最佳条件。结果显示,产朊假丝酵母能高效利用纤维二糖;37℃、单纯酶解100 g瞬时弹射式蒸汽爆破预处理玉米秸秆可产32.16 g总糖,同步酶解发酵后干物质降解率51.61%,总糖利用率为84.1%;相同温度条件下三因素三水平正交试验结果 :料水比1:7,纤维素酶复合物20 FPU,添加1%(NH_4)_2SO_4,发酵96 h后产品真蛋白质含量为14.66%。表明得到产朊假丝酵母最优同步酶解发酵条件,且该条件下转化玉米秸秆生产生物饲料具有经济可行性。
To improve the economic feasibility of producing protein feedstuff from corn straw, we optimized the process of synchronous saccharification and fermentation(SSF) with Instant Catapult Steam Explosion(ICSE) on pretreated corn straw. Candida utilis BNCC336517 strain was screened using synthetic medium and straw enzymatic hydrolysate. The effects of enzymatic hydrolysis and SSF at different temperatures on the sugar conversion of ICSE pretreated straw were compared. Furthermore, orthogonal experiments were designed to obtain the optimum conditions for conversion of true protein from corn straw. Results showed that Candida utilis strain was obtained with cellobiose as the sole carbon source. Under the condition of 37 ℃, 100 g ICSE pretreatment of corn stalk can produce 32.16 g total sugar,the dry matter degradation rate under SSF conditions was 51.61%. The results of orthogonal experiment of 3 factors and3 levels under the same temperature conditions: the ratio of material to water 1 : 7, cellulase complex 20 FPU, adding1%(NH_4)_2 SO_4, the true protein content of the product after fermentation for 96 h was 14.66%. The optimal simultaneous enzymatic hydrolysis conditions of Candida utilis were obtained, and the conversion of corn stalks to produce biological feed under such conditions was economically feasible.
To improve the economic feasibility of producing protein feedstuff from corn straw, we optimized the process of synchronous saccharification and fermentation(SSF) with Instant Catapult Steam Explosion(ICSE) on pretreated corn straw. Candida utilis BNCC336517 strain was screened using synthetic medium and straw enzymatic hydrolysate. The effects of enzymatic hydrolysis and SSF at different temperatures on the sugar conversion of ICSE pretreated straw were compared. Furthermore, orthogonal experiments were designed to obtain the optimum conditions for conversion of true protein from corn straw. Results showed that Candida utilis strain was obtained with cellobiose as the sole carbon source. Under the condition of 37 ℃, 100 g ICSE pretreatment of corn stalk can produce 32.16 g total sugar,the dry matter degradation rate under SSF conditions was 51.61%. The results of orthogonal experiment of 3 factors and3 levels under the same temperature conditions: the ratio of material to water 1 : 7, cellulase complex 20 FPU, adding1%(NH_4)_2 SO_4, the true protein content of the product after fermentation for 96 h was 14.66%. The optimal simultaneous enzymatic hydrolysis conditions of Candida utilis were obtained, and the conversion of corn stalks to produce biological feed under such conditions was economically feasible.
引文
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[19]胡艳丽,王克然.饲料中真蛋白的测定及其意义[C]//河南省畜牧兽医学会理事会会议暨学术研讨会,2008.
[20]胡艳丽,王克然.饲料中真蛋白的测定[J].河南畜牧兽医(综合版),2007(10):31-32.
[21]P Béguin,Aubert J P.The biological degradation of cellulose[J].Fems Microbiology Reviews,1993,13(1):25.
[22]高晗旸,孙清,吕济敏,等.利用紫外诱变绿色木霉对玉米秸秆进行酶解糖化的研究[J].沈阳农业大学学报,2013,44(2):237-240.
[23]陈合,余建军,舒国伟,等.产朊假丝酵母1807高产培养基的优化[J].中国调味品,2010,35(7):52-54.
[24]Kurcz A,Blazejak S,Kot A M,et al.Application of Industrial Wastes for the Production of Microbial Single-Cell Protein by Fodder Yeast Candida utilis[J].Waste and Biomass Valorization,2018(2):57-64.
[2]高梦祥,许育彬,熊雷锋,等.玉米秸秆的综合利用途径[J].陕西农业科学,2000(7):29-31.
[3]杨兴,张起凯,李萍.玉米秸秆预处理技术及资源化研究进展[J].辽宁农业科学,2009(6):35-37.
[4]Collins,Pritchard R M.Alternate day supplementation of corn stalk diets with soybean meal or corn gluten meal fed to ruminants[J].Journal of Animal Science,1992,70(12):3899.
[5]柴磊,杨美荣,寇秋霜,等.玉米秸秆发酵饲料在养殖业中的应用现状[J].畜禽业,2018,29(10):23-24.
[6]曹红梅,刘文文.玉米秸秆生物发酵饲料的最新研究进展[J].中国饲料,2016(15):24-27.
[7]Sun S,Sun S,Cao X,et al.The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials[J].Bioresource Technology,2015,199:49-58.
[8]Manners D J.Cellulose and its derivatives.Chemistry,biochemistry and applications[J].Febs Letters,1987,219(2):488-489.
[9]韩明鹏,高永革,王成章,等.玉米秸秆发酵饲料的研究进展[J].江苏农业科学,2010,(2):242-245.
[10]黄俊华,李仕坚,严高彰,等.玉米秸秆微贮技术与青贮技术生产奶牛粗饲料的对比试验研究[J].安徽农学通报(下半月刊),2009,15(20):33-34,42.
[11]He L,Wu H,Wang G,et al.The effects of including corn silage,corn stalk silage,and corn grain in finishing ration of beef steers on meat quality and oxidative stability[J].Meat Science,2018,139:142-148.
[12]王彪,徐景会,宋丽萍.秸秆微贮饲料的制作与利用[J].饲料博览,1995(6):35-36.
[13]Sun Z,Li X,Liu L.Effect of steam explosion on solid-state fermentation of maize stalk by Penicillium decumbens and Phanerochaete chrysosporium for animal feed production[J].Journal of Animal Physiology&Animal Nutrition,2017,(6):596-599.
[14]马鹏,冯冲,刘会丽,等.瞬时弹射式蒸汽爆破对玉米秸秆化学结构的影响[J].河南农业大学学报,2018,52(1):85-90.
[15]郭照宙,许灵敏,宋建楼,等.产朊假丝酵母功能的探究及应用[J].饲料博览,2016(3):33-35,39.
[16]赖智乐,常春,马晓建,等.汽爆玉米秸秆同步糖化发酵产乙醇的工艺优化[J].农业工程学报,2010,26(7):250-254.
[17]刘剑虹,陈庆森,陈剑勇,等.酸处理玉米秸秆生物转化单细胞蛋白的研究[J].天津商业大学学报,2001,21(3):1-5.
[18]何欢,吕美,甄广田,等.利用玉米秸秆生产单细胞蛋白关键技术研究[J].沈阳师范大学学报(自然科学版),2016,34(3):311-315.
[19]胡艳丽,王克然.饲料中真蛋白的测定及其意义[C]//河南省畜牧兽医学会理事会会议暨学术研讨会,2008.
[20]胡艳丽,王克然.饲料中真蛋白的测定[J].河南畜牧兽医(综合版),2007(10):31-32.
[21]P Béguin,Aubert J P.The biological degradation of cellulose[J].Fems Microbiology Reviews,1993,13(1):25.
[22]高晗旸,孙清,吕济敏,等.利用紫外诱变绿色木霉对玉米秸秆进行酶解糖化的研究[J].沈阳农业大学学报,2013,44(2):237-240.
[23]陈合,余建军,舒国伟,等.产朊假丝酵母1807高产培养基的优化[J].中国调味品,2010,35(7):52-54.
[24]Kurcz A,Blazejak S,Kot A M,et al.Application of Industrial Wastes for the Production of Microbial Single-Cell Protein by Fodder Yeast Candida utilis[J].Waste and Biomass Valorization,2018(2):57-64.