矮紫堇化学成分的UHPLC-Q-TOF-MS/MS分析
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摘要
目的:采用超高效液相色谱仪与四极杆飞行时间串联质谱仪(UHPLC-Q-TOF-MS/MS)对矮紫堇中化学成分进行快速分析和鉴别。方法:采用YMC-ULtra HT Pro C18色谱柱(100 mm×3 mm,2μm),以甲醇和0.5%甲酸-水的流动相进行快速的梯度洗脱,流速0.4 m L/mi,柱温32℃。质谱定性采用电喷雾离子源(ESI)的飞行时间质谱,正、负离子模式下采集数据,质量扫描范围m/z 100~1000。结果:共鉴别出矮紫堇中的10个生物碱类成分、2个黄酮类成分。结论:UHPLC-Q-TOF-MS/MS方法能快速鉴别矮紫堇中的化学成分,为矮紫堇的药效物质研究奠定基础。
Objective: To analyze and identify the chemical constituents in Corydalis hendersonii Hemsl by UHPLC-Q-TOF-MS.Methods: The analysis was performed on a YMC-ULtra HT Pro C18 analytical column with a gradient solvent system of methanol-water containing 0. 5% formic acid at the flow rate of 0. 4 m L/min. The column temperature was at 30 ℃. Time-of-flight mass spect-rometer(TOF/MS) and electrospray ion source(ESI) was applied for qualitative analysis under the positive and negative ion mode,the mass scan range was m/z100 ~ 1000. Result: Ten alkaloid compounds and two flavone compounds were identified from C. hendersonii Hemsl. Conclusion: Using UHPLC-Q-TOF-MS/MS method can quickly identify the chemical components of C. hendersonii Hemsl which paves a way for further studying the pharmacodynamic material basis of C. hendersonii Hemsl.
Objective: To analyze and identify the chemical constituents in Corydalis hendersonii Hemsl by UHPLC-Q-TOF-MS.Methods: The analysis was performed on a YMC-ULtra HT Pro C18 analytical column with a gradient solvent system of methanol-water containing 0. 5% formic acid at the flow rate of 0. 4 m L/min. The column temperature was at 30 ℃. Time-of-flight mass spect-rometer(TOF/MS) and electrospray ion source(ESI) was applied for qualitative analysis under the positive and negative ion mode,the mass scan range was m/z100 ~ 1000. Result: Ten alkaloid compounds and two flavone compounds were identified from C. hendersonii Hemsl. Conclusion: Using UHPLC-Q-TOF-MS/MS method can quickly identify the chemical components of C. hendersonii Hemsl which paves a way for further studying the pharmacodynamic material basis of C. hendersonii Hemsl.
引文
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[3]许文,傅志勤,林婧,等.UPLC-MS/MS法同时测定三叶青中10种黄酮类成分[J].药学学报,2014,49(12):1 711-1 717.
[4]宋建平,许虎,陈菲,等.罗布麻叶黄酮类成分的UPLC-Q-TOF-MS分析[J].中药材,2014,37(7):1 199-1 204.
[5]卿志星,程辟,曾建国.博落回中生物碱质谱裂解规律研究进展[J].中草药,2013,44(20):2 929-2 939.
[6]丁波.延胡索物质基础及其主要活性单体体内代谢的LC-DAD-MS/MS研究[D].上海:第二军医大学,2007.
[7]李宇航,戴海学,汪明明,等.槲皮素的电喷雾离子阱质谱分析[J].质谱学报,2009,30(6):374-378.
[8]李军茂,何明珍,欧阳辉,等.超高效液相色谱与飞行时间质谱联用快速鉴别木芙蓉叶的化学成分[J].中国药学杂志,2016,51(14):1 162-1 168.
[9]苏靖,戴荣继.液质联用技术在中药研究中的应用[J].生命科学仪器,2014,12(Z1):38-42.
[10]高燕萍,吴强,梁健,等.矮紫堇生物碱成分[J].中国实验方剂学杂志,2016,22(17):67-70.
[11]高燕萍,吴强,梁健,等.藏族药矮紫堇化学成分分离鉴定[J].中国实验方剂学杂志,2016,22(18):60-63.
[12]刘璐,吴立军,杨春娟.紫堇属植物中异喹啉类生物碱的化学结构和生物活性的研究进展[J].实用药物与临床,2016,19(3):371-380.