白细胞介素18在非小细胞肺癌转移中的作用机制
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摘要
目的检测白细胞介素(IL)-18在非小细胞肺癌高、低转移株表达的差异。方法采用半定量RT-PCR及Western印迹技术,检测IL-18在非小细胞肺癌高、低转移株的差异表达;构建IL-18 cDNA的重组质粒,同时把IL-18基因导入非小细胞肺癌高、低转移株,观察IL-18对肿瘤细胞体外转移能力的影响。结果与非小细胞肺癌低转移株相比,IL-18 mRNA和蛋白均只能在非小细胞肺癌高转移株检测到,表达量分别为(5.41±1.20)、(3.98±2.01)。将IL-18基因导入非小细胞肺癌低转移株后,转染细胞的体外迁移能力显著上调,为空白对照组的3倍左右。结论非小细胞肺癌高转移株可组成性表达IL-18,且IL-18表达促进非小细胞肺癌的转移。
Objective To compare interleukin( IL)-18 expression between high and low metastatic plant in non-small cell lung cancer( NSCLC). Methods Semi-quantitative RT-PCR and Western blot analysis were used to compare IL-18 expressions between high and low metastatic plant in NSCLC; IL-18 cDNA recombinant plasmid was published,at the same time the IL-18 genes were leaded in NSCLC with high and low strain. IL-18 effects on tumor cells in vitro metastasis ability were observed. Results Compared with NSCLC with low transfer plant,IL-18 mRNA and protein were only detected in high metastatic strains of NSCLC,expression quantity were( 5. 41 ± 1. 20)( 3.98 ±2.01) respectively,after introducing IL-18 genes in NSCLC into low metastatic plant,metastasis cells in vitro migration ability to rise significantly,about 3 times as the blank control group. Conclusions With IL-18 expression in high metastatic plants of NSCLC,IL-18expression promotes the metastasis of NSCLC cells.
Objective To compare interleukin( IL)-18 expression between high and low metastatic plant in non-small cell lung cancer( NSCLC). Methods Semi-quantitative RT-PCR and Western blot analysis were used to compare IL-18 expressions between high and low metastatic plant in NSCLC; IL-18 cDNA recombinant plasmid was published,at the same time the IL-18 genes were leaded in NSCLC with high and low strain. IL-18 effects on tumor cells in vitro metastasis ability were observed. Results Compared with NSCLC with low transfer plant,IL-18 mRNA and protein were only detected in high metastatic strains of NSCLC,expression quantity were( 5. 41 ± 1. 20)( 3.98 ±2.01) respectively,after introducing IL-18 genes in NSCLC into low metastatic plant,metastasis cells in vitro migration ability to rise significantly,about 3 times as the blank control group. Conclusions With IL-18 expression in high metastatic plants of NSCLC,IL-18expression promotes the metastasis of NSCLC cells.
引文
1 赖远波,陈淡森.揭阳地区汉族人群白细胞介素18基因启动子多态性与结核病的相关性[J].广东医学,2013;34(16):2553-5.
2 周喜云.大肠癌术后患者营养状态与血清中肿瘤坏死因子-α、IL-18和IL-23的关系[J].中国老年学杂志,2013;33(17):4340-1.
3 闭肇龙,侯恩存,吴波,等.血清IL-18对肝细胞癌患者的预后价值[J].中国现代医学杂志,2013;23(22):34-7.
4 邵奇,王宁.白细胞介素-18与肿瘤侵袭转移相关性的研究现状[J].中华肿瘤防治杂志,2012;19(7):547-52.
5 Bian C,Wang ZC,Yang JL,et al.Up-regulation of interleukin-23 induces persistent allodynia via CX3CL1 and interleukin-18 signaling in the rat spinal cord after tetanic sciatic stimulation[J].Brain Behav Immun,2013;1591(13):596-5.
6 陈志芬,邓长生,冯茂辉,等.人白细胞介素-15与18协同增强肿瘤浸润淋巴细胞体内对结肠癌细胞的杀伤功能[J].中华实验外科杂志,2010;27(8):1041-5.
7 蒋常文,夏春波,李鸿文,等.外源性IL-18基因表达对C6胶质瘤细胞侵袭力和克隆形成的影响[J].中华临床医师杂志(电子版),2010;4(10):2001-3.
8 杨文刚,刘俊华,陈嘉宝,等.白细胞介素-18mRNA在非小细胞肺癌中的表达及其临床意义[J].现代肿瘤医学,2011;19(12):2426-8.
9 蒋代凤,邢桂春,陈筱潇,等.白细胞介素18在肺癌细胞的表达及其在肿瘤转移中功能的初步探讨[J].免疫学杂志,2003;19(5):355-60.
10 蒋常文,夏春波,李鸿文,等外源性IL-18基因转染联合顺铂对胶质瘤C6细胞凋亡的诱导作用[J].山东医药,2010;50(31):13-5.
11 张在云,李晓梅,王涓冬.IL-18基因修饰对肺癌细胞来源exosome诱导杀伤肿瘤细胞作用的影响[J].中国病理生理杂志,2011;27(9):1758-61.
12 Krzanowski M,Janda K.Dumnicka P-Relationship between aortic pulse wave velocity,selected proinflammatory cytokines,and vascular calcification parameters in peritoneal dialysis patients[J].J Hypertens,2014;32(1):142-8.
2 周喜云.大肠癌术后患者营养状态与血清中肿瘤坏死因子-α、IL-18和IL-23的关系[J].中国老年学杂志,2013;33(17):4340-1.
3 闭肇龙,侯恩存,吴波,等.血清IL-18对肝细胞癌患者的预后价值[J].中国现代医学杂志,2013;23(22):34-7.
4 邵奇,王宁.白细胞介素-18与肿瘤侵袭转移相关性的研究现状[J].中华肿瘤防治杂志,2012;19(7):547-52.
5 Bian C,Wang ZC,Yang JL,et al.Up-regulation of interleukin-23 induces persistent allodynia via CX3CL1 and interleukin-18 signaling in the rat spinal cord after tetanic sciatic stimulation[J].Brain Behav Immun,2013;1591(13):596-5.
6 陈志芬,邓长生,冯茂辉,等.人白细胞介素-15与18协同增强肿瘤浸润淋巴细胞体内对结肠癌细胞的杀伤功能[J].中华实验外科杂志,2010;27(8):1041-5.
7 蒋常文,夏春波,李鸿文,等.外源性IL-18基因表达对C6胶质瘤细胞侵袭力和克隆形成的影响[J].中华临床医师杂志(电子版),2010;4(10):2001-3.
8 杨文刚,刘俊华,陈嘉宝,等.白细胞介素-18mRNA在非小细胞肺癌中的表达及其临床意义[J].现代肿瘤医学,2011;19(12):2426-8.
9 蒋代凤,邢桂春,陈筱潇,等.白细胞介素18在肺癌细胞的表达及其在肿瘤转移中功能的初步探讨[J].免疫学杂志,2003;19(5):355-60.
10 蒋常文,夏春波,李鸿文,等外源性IL-18基因转染联合顺铂对胶质瘤C6细胞凋亡的诱导作用[J].山东医药,2010;50(31):13-5.
11 张在云,李晓梅,王涓冬.IL-18基因修饰对肺癌细胞来源exosome诱导杀伤肿瘤细胞作用的影响[J].中国病理生理杂志,2011;27(9):1758-61.
12 Krzanowski M,Janda K.Dumnicka P-Relationship between aortic pulse wave velocity,selected proinflammatory cytokines,and vascular calcification parameters in peritoneal dialysis patients[J].J Hypertens,2014;32(1):142-8.