不同针灸方法对膜迷路积水模型豚鼠听力及耳蜗形态结构的影响
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摘要
目的探讨不同针灸方法干预对豚鼠膜迷路积水的效应差异。方法 50只豚鼠随机分为空白组、模型组、针刺组、艾灸组、电针组,每组10只。采用腹腔注射醛固酮的方法诱导膜迷路积水模型。各治疗组分别在"百会""听宫"实施相应穴位刺激,每日1次,连续治疗10 d。治疗结束后,行听觉脑干诱发电位测试,观察听阈值。HE染色法观察耳蜗积水程度,计算蜗管横截面积与蜗管加前庭阶横截面积之和的比值(R值)。结果 1针刺组、艾灸组、电针组听阈值均较模型组降低(P<0.01),电针组听阈值较艾灸组、针刺组降低(P<0.01),艾灸组听阈值较针刺组降低(P<0.05)。2针刺组、艾灸组、电针组R值均较模型组降低(P<0.01),电针组R值较针刺组、艾灸组降低(P<0.01,P<0.05),艾灸组R值较针刺组降低(P<0.01)。结论针刺、艾灸、电针刺激"百会""听宫"均能减轻豚鼠膜迷路积水,并且改善耳蜗听功能。电针表现出最好的效应,优于艾灸、针刺;艾灸优于针刺。
Objective This study was conducted to compare the efficacy of acupuncture,moxibustion and electroacupuncture on guinea pig models of cochlear endolymphatic hydrops. Methods Fifty healthy male guinea pigs( body weight 200-250 g) were randomly divided into five groups: the normal group,model group,acupuncture group,moxibustion group,and electro-acupuncture group( n = 10 in each group). Forty guinea pigs received a once-daily intraperitoneal injection of aldosterone in a dose of 100 μg /100 g / d for 5 days to induce endolymphatic hydrops. After a 2-month interval, the animals of the acupuncture, moxibustion and electro-acupuncture groups were treated with corresponding acupoints stimulation at points Baihui( GV 20) and Tinggong( SI 19) once a day for 10 days,respectively.We measured the hearing threshold of the auditory brainstem response( ABR) and evaluated pathologically the degree of hydrops using hematoxylin-eosin staining,and then the ratio of scala media( SM) area / [SM + scala vestibuli( SV)area] was calculated. Results 1. The hearing threshold of ABR in the acupuncture,moxibustion and electro-acupuncturegroups were all significantly lower than that of the model group( P < 0. 01). The ABR threshold in the electro-acupuncture group was significantly decreased in comparison with that of the acupuncture and moxibustion groups( P < 0. 01 for both).The hearing threshold of the moxibustion group was significantly lower than that of the acupuncture group( P < 0. 05). 2.The ratio of SM /( SM + SV) in the acupuncture,moxibustion and electro-acupuncture groups were all significantly lower than that of the model group( P < 0. 01). The ratio in the electro-acupuncture group was significantly decreased in comparison with that of the acupuncture and moxibustion groups( P < 0. 01,P < 0. 05). The ratio of the moxibustion group was significantly lower than that of the acupuncture group( P < 0. 01). Conclusions Our findings suggest that all acupuncture,moxibustion and electro-acupuncture at the acupoints Baihui( GV 20) and Tinggong( SI 19) can significantly suppress the development of endolymphatic hydrops and improve the cochlear function in guinea pigs. Electroacupuncture is the most effective in this experiment,and moxibustion therapy shows better effect than acupuncture.
Objective This study was conducted to compare the efficacy of acupuncture,moxibustion and electroacupuncture on guinea pig models of cochlear endolymphatic hydrops. Methods Fifty healthy male guinea pigs( body weight 200-250 g) were randomly divided into five groups: the normal group,model group,acupuncture group,moxibustion group,and electro-acupuncture group( n = 10 in each group). Forty guinea pigs received a once-daily intraperitoneal injection of aldosterone in a dose of 100 μg /100 g / d for 5 days to induce endolymphatic hydrops. After a 2-month interval, the animals of the acupuncture, moxibustion and electro-acupuncture groups were treated with corresponding acupoints stimulation at points Baihui( GV 20) and Tinggong( SI 19) once a day for 10 days,respectively.We measured the hearing threshold of the auditory brainstem response( ABR) and evaluated pathologically the degree of hydrops using hematoxylin-eosin staining,and then the ratio of scala media( SM) area / [SM + scala vestibuli( SV)area] was calculated. Results 1. The hearing threshold of ABR in the acupuncture,moxibustion and electro-acupuncturegroups were all significantly lower than that of the model group( P < 0. 01). The ABR threshold in the electro-acupuncture group was significantly decreased in comparison with that of the acupuncture and moxibustion groups( P < 0. 01 for both).The hearing threshold of the moxibustion group was significantly lower than that of the acupuncture group( P < 0. 05). 2.The ratio of SM /( SM + SV) in the acupuncture,moxibustion and electro-acupuncture groups were all significantly lower than that of the model group( P < 0. 01). The ratio in the electro-acupuncture group was significantly decreased in comparison with that of the acupuncture and moxibustion groups( P < 0. 01,P < 0. 05). The ratio of the moxibustion group was significantly lower than that of the acupuncture group( P < 0. 01). Conclusions Our findings suggest that all acupuncture,moxibustion and electro-acupuncture at the acupoints Baihui( GV 20) and Tinggong( SI 19) can significantly suppress the development of endolymphatic hydrops and improve the cochlear function in guinea pigs. Electroacupuncture is the most effective in this experiment,and moxibustion therapy shows better effect than acupuncture.
引文
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[2]张良,尹亚东,刘书红.中西医结合治疗内耳眩晕体会[J].中国中医急症,2014,23(2):284-285.
[3]李彦,田兴德,涂德根.美尼埃病的非手术治疗[J].长江大学学报(自科版),2013,10(12):99-102.
[4]崔彪,时海波,于栋祯.Meniett低压脉冲发生器治疗梅尼埃病的疗效分析[J].临床耳鼻咽喉头颈外科杂志,2014,28(1):6-8.
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[8]张晓哲.电针针刺治疗耳鸣临床疗效对比研究[J].中国针灸,2002,22(2):91-92.
[9]周青梅.针刺联合艾灸治疗痰浊中阻型梅尼埃病70例临床观察[J].实用中医内科杂志,2013,27(14):147-149.
[10]管烨.针灸治疗美尼尔综合症的研究进展[J].针灸临床杂志,2012,28(9):66-68.
[11]叶家豪,李涛.美尼尔病的中医研究近况[J].陕西中医,2012,33(4):501-503.
[12]蒋子栋,张连山.醛固酮诱发豚鼠双耳膜迷路积水[J].中国医学科学院学报,2002,24(5):501-504.
[13]Dunnebier EA,Segenhout JM,Wit HP,et al.Two-phase endolymphatic hydrops:a new dynamic guinea pig model[J].Acta Otolaryngol,1997,117(1):13-19.
[14]林文注,王佩.实验针灸学[M].上海:上海科学技术出版社出版,1999:289.
[15]贾贤浩,梁琴,池涨才,等.豚鼠内淋巴积水早期低频听力损失的形态学变化[J].生理学报,2012,64(1):48-54.
[16]徐亚平,顾健,刘海斌,等.内耳形态学和生理指标对缺铁性肾虚耳聋大鼠模型的评价作用[J].中国实验动物学报,2012,20(3):2 9-32.
[17]李琦.水通道蛋白1在豚鼠耳蜗形态改变时的表达及其相关因素分析[D].中国人民解放军军医进修学院,2006.
[18]杨丽君,孙立新.针灸治疗内耳眩晕症45例临床观察[J].中国医药导报,2010,7(35):68-69.
[19]王福海.中药联合针灸治疗梅尼埃病30例[J].中医研究,2012,25(11):72-73.
[20]熊锡元,娄明志.116例梅尼埃病听力损失的临床分析[J].医学新知杂志,2006,16(2):120-122.
[21]徐志勇,刘兆华.豚鼠膜迷路积水早期的听功能改变[J].中华耳鼻咽喉科杂志,2002,37(3):226-227.
[22]贾君君,解秸萍,农艳,等.传统手针与电针对比研究现状[J].针灸临床杂志,2007,23(4):62-63.
[23]史美育,曾兆麟,施建蓉,等.针刺对豚鼠皮层听觉中枢兴奋性的影响[J].上海针灸杂志,1997,16(3):29-31.
[24]后婕,戴艳红,佘万东.盐皮质激素受体对糖皮质激素的影响及其在自身免疫性内耳疾病中的作用[J].听力学及言语疾病杂志,2014,22(3):313-316.
[25]杨晨,杨艳,刁明芳,等.持续静脉给予肾上腺素对大鼠听力以及下丘脑-垂体-肾上腺轴相关激素的影响[J].中国耳鼻咽喉颅底外科杂志,2014,20(1):21-24.
[2]张良,尹亚东,刘书红.中西医结合治疗内耳眩晕体会[J].中国中医急症,2014,23(2):284-285.
[3]李彦,田兴德,涂德根.美尼埃病的非手术治疗[J].长江大学学报(自科版),2013,10(12):99-102.
[4]崔彪,时海波,于栋祯.Meniett低压脉冲发生器治疗梅尼埃病的疗效分析[J].临床耳鼻咽喉头颈外科杂志,2014,28(1):6-8.
[5]刘立公,顾杰.眩晕证的古代针灸治疗特点[J].中医文献杂志,1997,(2):6-7.
[6]茅利玉,陆海娟,沈小红,等.针刺治疗梅尼埃病疗效观察[J].上海针灸杂志,2014,33(6):575.
[7]许舜沛,贺君,陈裕,等.针灸治疗梅尼埃病国内外研究概况[J].针灸临床杂志,2001,17(12):44-45.
[8]张晓哲.电针针刺治疗耳鸣临床疗效对比研究[J].中国针灸,2002,22(2):91-92.
[9]周青梅.针刺联合艾灸治疗痰浊中阻型梅尼埃病70例临床观察[J].实用中医内科杂志,2013,27(14):147-149.
[10]管烨.针灸治疗美尼尔综合症的研究进展[J].针灸临床杂志,2012,28(9):66-68.
[11]叶家豪,李涛.美尼尔病的中医研究近况[J].陕西中医,2012,33(4):501-503.
[12]蒋子栋,张连山.醛固酮诱发豚鼠双耳膜迷路积水[J].中国医学科学院学报,2002,24(5):501-504.
[13]Dunnebier EA,Segenhout JM,Wit HP,et al.Two-phase endolymphatic hydrops:a new dynamic guinea pig model[J].Acta Otolaryngol,1997,117(1):13-19.
[14]林文注,王佩.实验针灸学[M].上海:上海科学技术出版社出版,1999:289.
[15]贾贤浩,梁琴,池涨才,等.豚鼠内淋巴积水早期低频听力损失的形态学变化[J].生理学报,2012,64(1):48-54.
[16]徐亚平,顾健,刘海斌,等.内耳形态学和生理指标对缺铁性肾虚耳聋大鼠模型的评价作用[J].中国实验动物学报,2012,20(3):2 9-32.
[17]李琦.水通道蛋白1在豚鼠耳蜗形态改变时的表达及其相关因素分析[D].中国人民解放军军医进修学院,2006.
[18]杨丽君,孙立新.针灸治疗内耳眩晕症45例临床观察[J].中国医药导报,2010,7(35):68-69.
[19]王福海.中药联合针灸治疗梅尼埃病30例[J].中医研究,2012,25(11):72-73.
[20]熊锡元,娄明志.116例梅尼埃病听力损失的临床分析[J].医学新知杂志,2006,16(2):120-122.
[21]徐志勇,刘兆华.豚鼠膜迷路积水早期的听功能改变[J].中华耳鼻咽喉科杂志,2002,37(3):226-227.
[22]贾君君,解秸萍,农艳,等.传统手针与电针对比研究现状[J].针灸临床杂志,2007,23(4):62-63.
[23]史美育,曾兆麟,施建蓉,等.针刺对豚鼠皮层听觉中枢兴奋性的影响[J].上海针灸杂志,1997,16(3):29-31.
[24]后婕,戴艳红,佘万东.盐皮质激素受体对糖皮质激素的影响及其在自身免疫性内耳疾病中的作用[J].听力学及言语疾病杂志,2014,22(3):313-316.
[25]杨晨,杨艳,刁明芳,等.持续静脉给予肾上腺素对大鼠听力以及下丘脑-垂体-肾上腺轴相关激素的影响[J].中国耳鼻咽喉颅底外科杂志,2014,20(1):21-24.