虫酰肼和灭幼脲对锈色粒肩天牛氧化酶和解毒酶活性的影响
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摘要
为探究虫酰肼和灭幼脲对锈色粒肩天牛Apriona swainsoni(Hope)生理机制的影响,用经灭幼脲(T1)和虫酰肼(T2)处理的木屑饲喂锈色粒肩天牛幼虫,测定天牛幼虫的过氧化氢酶(CAT),超氧化物歧化酶(SOD),谷胱甘肽S转移酶(GSTs)和羧酸酯酶(Car E)活性,每12 h取样,持续72 h。结果显示:T1处理CAT活性先升高,24 h后降低;T2处理CAT活性降低;与对照组相比,T1和T2处理SOD活性、GSTs活性显著增高(P<0.05),Car E活性则无明显差异。推测锈色粒肩天牛幼虫GSTs活性和虫体抗性的产生有关,GSTs活性可能是天牛幼虫的抗性标识物。
To research the influence of tebufenozide and chlorbenzuron on the physi ological mechanism of Apriona swainsoni(Hope), A. swainsoni larvae were fed with sawdust treated by chlorbenzuron(T1) and tebufenozide(T2). These were then sampled to determine enzymatic activity of catalase(CAT), superoxide dismutase(SOD), glutathione S transferases(GSTs), and carboxylesterase(Car E) in larvae at intervals of 12 h to 72 h in succession. Results showed that after treatment with chlorbenzuron, the enzymatic activity of CAT increased first and then decreased 24 h later. Enzymatic activity of SOD maintained a high level. Also, after treatment by tebufenozide, the enzymatic activity of CAT decreased first with enzymatic activity of SOD being higher than the control. After treatment by two types of the pesticides, enzymatic activity of GSTs was higher than the control with no differences of Car E activity with the control. Therefore, enzymatic activity of GSTs in A. swainsoni was assumed to be associated with resistance generated in the larva and was likely to be a resistance marker of the larvae.
To research the influence of tebufenozide and chlorbenzuron on the physi ological mechanism of Apriona swainsoni(Hope), A. swainsoni larvae were fed with sawdust treated by chlorbenzuron(T1) and tebufenozide(T2). These were then sampled to determine enzymatic activity of catalase(CAT), superoxide dismutase(SOD), glutathione S transferases(GSTs), and carboxylesterase(Car E) in larvae at intervals of 12 h to 72 h in succession. Results showed that after treatment with chlorbenzuron, the enzymatic activity of CAT increased first and then decreased 24 h later. Enzymatic activity of SOD maintained a high level. Also, after treatment by tebufenozide, the enzymatic activity of CAT decreased first with enzymatic activity of SOD being higher than the control. After treatment by two types of the pesticides, enzymatic activity of GSTs was higher than the control with no differences of Car E activity with the control. Therefore, enzymatic activity of GSTs in A. swainsoni was assumed to be associated with resistance generated in the larva and was likely to be a resistance marker of the larvae.
引文
[1]卢希平,杨忠岐,孙绪艮,等.利用花绒寄甲防治锈色粒肩天牛[J].林业科学,2011,47(10):116-121.LU Xiping,YANG Zhongqi,SUN Xugen,et al.Biological control of Apriona swainsoni(Coleoptera:Cerambycidae)by releasing the parasitic beetle Dastarcus helophoroides(Coleoptera:Bothrideridae)[J].Sci Silv Sin,2011,47(10):116-121.
[2]周亚军,赵从志.锈色粒肩天牛的初步研究[J].河南农业大学学报,1987,21(1):65-70.ZHOU Yajun,ZHAO Congzhi.Preliminary studies on Apriona sweingeni(Hope)[J].Acta Agric Univ Henan,1987,21(1):65-70.
[3]孙丹萍.锈色粒肩天牛幼虫空间分布型及抽样技术研究[J].西北林学院学报,2007,22(3):120-123.SUN Danping.Study on the spatial distribution pattern and sampling techniques of the larvas of Apriona swainsoni(Hope)[J].J Northwest For Univ,2007,22(3):120-123.
[4]曹广春.小菜蛾Plutella xylostella(L.)对虫酰肼的抗性及其机理研究[D].南京:南京农业大学,2007.CAO Guangchun.Stuydies on Resistance of Diamondback Moth Plutella xylostella(L.)to Tebufenozide and the Mechanisms[D].Nanjing:Nanjing Agricultural University,2007.
[5]崔全敏,王开运,汪清民,等.2种虫酰肼类新化合物对5种鳞翅目害虫的生物活性[J].昆虫学报,2008,51(5):492-497.CUI Quanmin,WANG Kaiyun,WANG Qingmin,et al.Bioactivities of two tebufenozide derivatives against five lepidopteran pests[J].Acta Entomol Sin,2008,51(5):492-497.
[6]WELLINGA K,MULDER R,van DAALEN J J.Synthesis and laboratory evaluation of 1-(2,6-disubstituted benzoyl)-3-phenylureas,a new class of insecticides.1-(2,6-Dichlorobenzoyl)-3-phenylureas[J].J Agric Food Chem,1973,21(3):348-354.
[7]金敏娜,林欣大.保幼激素在昆虫中的分子作用机理[J].生态学报,2014,34(6):1362-1370.JIN Minna,LIN Xinda.Molecular mechanisms of the insect juvenile hormone[J].Acta Ecol Sin,2014,34(6):1362-1370.
[8]唐进根.灭幼脲在桑天牛Apriona germari(Hope)世代间的传递机制[D].南京:南京农业大学,2001.TANG Jingen.The Transfer Mode of Diflubenzuron(DFB)in Between Generations of Mulberry Borer Apriona germari(Hope)(Coleoptera:Cerambycisae)[D].Nanjing:Nanjing Agricultural University,2001.
[9]田铃.桑天牛保幼激素生物合成机制及其对生殖的调控[D].南京:南京农业大学,2008.TIAN Ling.The Study on Biosynthetic Mechanism of Juvenile Hormone in Apriona germari and Its Regulation on Reproducation[D].Nanjing:Nanjing Agricultural University,2008.
[10]杨荣萍,刘明辉,曾春,等.灭幼脲的作用机理及其在林业害虫防治中的应用[J].现代农业科技,2010(15):220-223.YANG Rongping,LIU Minghui,ZENG Chun,et al.mechanism of diflubenzuron and its application in control of forest pests[J].Mod Agric Sci Technol,2010(15):220-223.
[11]TASSOU K T,SCHULZ R.Low field-relevant tebufenozide concentrations affect reproduction in Chironomus riparius(Diptera:Chironomidae)in a long-term toxicity test[J].Environ Sci Pollut Res,2013,20(6):3735-3742.
[12]Mc CORD J M,FRIDOWICH I.Superoxide dismutase:an enzymatic funcation for erythrocuprein(hemocuprein)[J].J Biol Chem,1969,244(22):6049-6055.
[13]张永强.黄花蒿杀螨物质活性追踪及杀螨作用机理研究[D].重庆:西南大学,2008.ZHANG Yongqiang.Studies on Acaricidal Action Mechanism and Bio-guided Isolation of Bioactive Sub stance from Artemisia annua L.[D].Chongqing:Southwest University,2008.
[14]LE Baothanh,颜学武,许潜.桑天牛幼虫龄数林间识别研究[J].湖南林业科技,2014,41(1):7-13.LE Baothanh,YAN Xuewu,XU Qian.Study on larval feces and instar identification of Apriona germari(Hope)[J].J Hunan For Sci Technol,2014,41(1):7-13.
[15]王小艺,杨忠岐,唐艳龙,等.栗山天牛幼虫龄数和龄期的测定[J].昆虫学报,2012,55(5):575-584.WANG Xiaoyi,YANG Zhongqi,TANG Yanlong,et al.Determination of larval instar number and duration in the oak longhorn beetle,Massicus raddei(Coleoptera:Cerambycidae)[J].Acta Entomol Sin,2012,55(5):575-584.
[16]张海滨,王建伟,朱小峰,等.星天牛幼虫龄期的划分[J].应用昆虫学报,2011,48(3):688-694.ZHANG Haibin,WANG Jianwei,ZHU Xiaofeng,et al.Derermination of larval instars of citrus long-horned beetle,Anoplophora chinensis(Coleoptera:Cerambycidae)[J].Chin J Appl Entomol,2011,48(3):688-694.
[17]DHADIALLA T S,CARLSON G R,LE D P.New insecticides with ecdysteroidal and juvenile hormone activity[J].Annu Rev Entomol,1998,43:545-569.
[18]徐希宝.与谷胱甘肽S-转移酶相关联的棉铃虫对甲氧虫酰肼的抗性机制研究[D].北京:中国农业科学院,2014.XU Xibao.Mechanisms of Resistance to Methoxyfenozide Rekated to Glutathione S-transferase in Cotton Bollworm,Helicoverpa armigera[D].Beijing:Chinese Academy of Agricultural Sciences,2014.
[19]SMAGGHE G,DHADIALLA T S,DERYCKE S,et al.Action of the ecdysteroid agonist tebufenozide in susceptible and artificially sekected beet armyworm[J].Pest Manage Sci,1998,54(1):27-34.
[2]周亚军,赵从志.锈色粒肩天牛的初步研究[J].河南农业大学学报,1987,21(1):65-70.ZHOU Yajun,ZHAO Congzhi.Preliminary studies on Apriona sweingeni(Hope)[J].Acta Agric Univ Henan,1987,21(1):65-70.
[3]孙丹萍.锈色粒肩天牛幼虫空间分布型及抽样技术研究[J].西北林学院学报,2007,22(3):120-123.SUN Danping.Study on the spatial distribution pattern and sampling techniques of the larvas of Apriona swainsoni(Hope)[J].J Northwest For Univ,2007,22(3):120-123.
[4]曹广春.小菜蛾Plutella xylostella(L.)对虫酰肼的抗性及其机理研究[D].南京:南京农业大学,2007.CAO Guangchun.Stuydies on Resistance of Diamondback Moth Plutella xylostella(L.)to Tebufenozide and the Mechanisms[D].Nanjing:Nanjing Agricultural University,2007.
[5]崔全敏,王开运,汪清民,等.2种虫酰肼类新化合物对5种鳞翅目害虫的生物活性[J].昆虫学报,2008,51(5):492-497.CUI Quanmin,WANG Kaiyun,WANG Qingmin,et al.Bioactivities of two tebufenozide derivatives against five lepidopteran pests[J].Acta Entomol Sin,2008,51(5):492-497.
[6]WELLINGA K,MULDER R,van DAALEN J J.Synthesis and laboratory evaluation of 1-(2,6-disubstituted benzoyl)-3-phenylureas,a new class of insecticides.1-(2,6-Dichlorobenzoyl)-3-phenylureas[J].J Agric Food Chem,1973,21(3):348-354.
[7]金敏娜,林欣大.保幼激素在昆虫中的分子作用机理[J].生态学报,2014,34(6):1362-1370.JIN Minna,LIN Xinda.Molecular mechanisms of the insect juvenile hormone[J].Acta Ecol Sin,2014,34(6):1362-1370.
[8]唐进根.灭幼脲在桑天牛Apriona germari(Hope)世代间的传递机制[D].南京:南京农业大学,2001.TANG Jingen.The Transfer Mode of Diflubenzuron(DFB)in Between Generations of Mulberry Borer Apriona germari(Hope)(Coleoptera:Cerambycisae)[D].Nanjing:Nanjing Agricultural University,2001.
[9]田铃.桑天牛保幼激素生物合成机制及其对生殖的调控[D].南京:南京农业大学,2008.TIAN Ling.The Study on Biosynthetic Mechanism of Juvenile Hormone in Apriona germari and Its Regulation on Reproducation[D].Nanjing:Nanjing Agricultural University,2008.
[10]杨荣萍,刘明辉,曾春,等.灭幼脲的作用机理及其在林业害虫防治中的应用[J].现代农业科技,2010(15):220-223.YANG Rongping,LIU Minghui,ZENG Chun,et al.mechanism of diflubenzuron and its application in control of forest pests[J].Mod Agric Sci Technol,2010(15):220-223.
[11]TASSOU K T,SCHULZ R.Low field-relevant tebufenozide concentrations affect reproduction in Chironomus riparius(Diptera:Chironomidae)in a long-term toxicity test[J].Environ Sci Pollut Res,2013,20(6):3735-3742.
[12]Mc CORD J M,FRIDOWICH I.Superoxide dismutase:an enzymatic funcation for erythrocuprein(hemocuprein)[J].J Biol Chem,1969,244(22):6049-6055.
[13]张永强.黄花蒿杀螨物质活性追踪及杀螨作用机理研究[D].重庆:西南大学,2008.ZHANG Yongqiang.Studies on Acaricidal Action Mechanism and Bio-guided Isolation of Bioactive Sub stance from Artemisia annua L.[D].Chongqing:Southwest University,2008.
[14]LE Baothanh,颜学武,许潜.桑天牛幼虫龄数林间识别研究[J].湖南林业科技,2014,41(1):7-13.LE Baothanh,YAN Xuewu,XU Qian.Study on larval feces and instar identification of Apriona germari(Hope)[J].J Hunan For Sci Technol,2014,41(1):7-13.
[15]王小艺,杨忠岐,唐艳龙,等.栗山天牛幼虫龄数和龄期的测定[J].昆虫学报,2012,55(5):575-584.WANG Xiaoyi,YANG Zhongqi,TANG Yanlong,et al.Determination of larval instar number and duration in the oak longhorn beetle,Massicus raddei(Coleoptera:Cerambycidae)[J].Acta Entomol Sin,2012,55(5):575-584.
[16]张海滨,王建伟,朱小峰,等.星天牛幼虫龄期的划分[J].应用昆虫学报,2011,48(3):688-694.ZHANG Haibin,WANG Jianwei,ZHU Xiaofeng,et al.Derermination of larval instars of citrus long-horned beetle,Anoplophora chinensis(Coleoptera:Cerambycidae)[J].Chin J Appl Entomol,2011,48(3):688-694.
[17]DHADIALLA T S,CARLSON G R,LE D P.New insecticides with ecdysteroidal and juvenile hormone activity[J].Annu Rev Entomol,1998,43:545-569.
[18]徐希宝.与谷胱甘肽S-转移酶相关联的棉铃虫对甲氧虫酰肼的抗性机制研究[D].北京:中国农业科学院,2014.XU Xibao.Mechanisms of Resistance to Methoxyfenozide Rekated to Glutathione S-transferase in Cotton Bollworm,Helicoverpa armigera[D].Beijing:Chinese Academy of Agricultural Sciences,2014.
[19]SMAGGHE G,DHADIALLA T S,DERYCKE S,et al.Action of the ecdysteroid agonist tebufenozide in susceptible and artificially sekected beet armyworm[J].Pest Manage Sci,1998,54(1):27-34.