玉米胚芽粕清蛋白酶解产物的制备及其抗氧化活性和护肝作用
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摘要
为开发利用玉米胚芽粕资源,研究玉米胚芽粕清蛋白酶解产物的酶解工艺及其抗氧化活性和护肝活性。研究结果表明:采用酸性蛋白酶酶解玉米胚芽粕清蛋白,在酶解温度44.9℃,p H 3.6,加酶量3 093.8 U/g,酶解时间1.5 h条件下,玉米胚芽粕清蛋白水解物对1,1-二苯基-2-三硝基苯肼的清除率达到最优值为95.15%,水解度为43.13%。采用体外2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法、Fe2+螯合法抗氧化模型考察玉米胚芽粕清蛋白水解物抗氧化活性,结果表明其抗氧化活性与质量浓度呈正相关。体内抗氧化实验结果表明:对于酒精诱导的急性酒精性肝损伤模型小鼠,玉米胚芽粕清蛋白水解物能显著提高体内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)的含量,降低氧化产物丙二醛(MDA)的含量。同时,小鼠肝组织病理学切片结果表明,其对急性酒精性肝损伤模型小鼠的肝脏具有一定的保护作用,能够抑制酒精在体内代谢过程中产生的氧化应激。玉米胚芽粕清蛋白酶解物具有潜在的护肝作用。
In order to develop and utilize the resources of corn germ, we studied the enzymatic hydrolysis process and antioxidant activity of CGMAH(Corn, germ, meal, albumin, hydrolysates) of corn germ meal. The results showed that under the condition of the acidic protease hydrolysis of corn germ meal albumin, hydrolysis temperature 44 ℃, p H 6, enzyme dosage 3 093.8 U/g, enzymolysis time 1.5 h, corn germ albumin hydrolysate for DPPH scavenging rate was optimal. By using the method in vitro, such as ABTS and Fe2 +chelating, to investigate the antioxidant activity of corn germ protein hydrolysate. The results showed that the antioxidant activity was positively correlated with the concentration.The experimental results show that the in vivo antioxidant on oxidative damage of mice induced by alcohol, corn germ meal albumin hydrolysate could significantly increase the content of SOD in vivo, GSH-Px and GSH, decreased the content of oxidation products of MDA. At the same time, liver tissue pathological results showed that corn germ albumin hydrolysate has a protective effect on liver injury caused by alcohol.
In order to develop and utilize the resources of corn germ, we studied the enzymatic hydrolysis process and antioxidant activity of CGMAH(Corn, germ, meal, albumin, hydrolysates) of corn germ meal. The results showed that under the condition of the acidic protease hydrolysis of corn germ meal albumin, hydrolysis temperature 44 ℃, p H 6, enzyme dosage 3 093.8 U/g, enzymolysis time 1.5 h, corn germ albumin hydrolysate for DPPH scavenging rate was optimal. By using the method in vitro, such as ABTS and Fe2 +chelating, to investigate the antioxidant activity of corn germ protein hydrolysate. The results showed that the antioxidant activity was positively correlated with the concentration.The experimental results show that the in vivo antioxidant on oxidative damage of mice induced by alcohol, corn germ meal albumin hydrolysate could significantly increase the content of SOD in vivo, GSH-Px and GSH, decreased the content of oxidation products of MDA. At the same time, liver tissue pathological results showed that corn germ albumin hydrolysate has a protective effect on liver injury caused by alcohol.
引文
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[19]CHENG D,KONG H.The effect of Lycium barbarum polysaccharide on alcohol-induced oxidative stress in rats[J].Molecules,2011,16(3):2542-2550.
[20]ALBANO E.Alcohol oxidative stress and free radical damage[J].Proceedings of the Nutrition Society,2006,65(3):278-290.
[2]张秋琴,叶义杰,张敏,等.玉米胚芽油的生产现状与发展前景[J].农产品加工,2008,6(8):54-56.
[3]李丽,崔波,白伟芳,等.玉米胚蛋白的综合利用[J].食品与发酵科技,2010,24(2):16-18.
[4]魏炳栋,邱玉朗,于维,等.玉米胚芽粕的营养特性及其在动物生产中的应用[J].中国饲料,2014,15(22):11-16.
[5]PARRIS N,ROBERT A M,DAVID B J,et al.Protein distribution in commercial wet and dry milled corn germ[J].Journal of Agricultural and Food Chemistry,2006,54(13):4868-4872.
[6]薛颖,王联结,党艳妮,等.超声波辅助碱法提取玉米胚芽粕蛋白质工艺研究[J].食品工业科技,2015(2):248-252.
[7]张明占.玉米胚芽脱脂粕酶解条件及其产物抗氧化活性的研究[D].齐齐哈尔:齐齐哈尔大学,2013:55-56
[8]BRANDON-WARNER E,SCHRUM L W,SCHMIDT C M,et al.Rodent models of alcoholic liver disease:of mice and men[J].Alcohol,2012,46(8):715-725.
[9]LEE K,Yun B S.Hispidin analogs from the mushroom Inonotus xeranticus and their free radical scavenging activi-ty[J].Biorg Med Chem Lett,2006,16(9):2376-2379.
[10]YAMAGUCHI M,TAKADA M,NOZAKI O,et al.Preparation of corn peptide from corn gluten meal and its administration effect on alcohol metabolism in stroke-prone spontaneously hypertensjve rats[J].Journal of Nu-tritional Science and Vitaminology,1996,42(3):219-231.
[11]邱金东,杨昆.DPPH和ABTS法测定核桃仁的体外抗氧化活性[J].中成药,2008,30(8):1215-1216.
[12]张彦芬,秘尧,何奇龙,等.FH/Wjd大鼠酒精性肝损伤模型探讨[J].中国实验动物学报,2014,22(5):59-62.
[13]肖娟,张瑞芬,黄菲,等.两种不同的酒精摄入方式诱导的小鼠酒精性肝损伤模型比较[J].中国比较医学杂志,2016,26(6):11-17.
[14]孙红,于得伟,崔志勇,等.玉米肽对动物实验性肝损伤的保护作用[J].中药药理与临床,2002,18(3):10-11.
[15]向进乐,李晨露,郭帅,等.红枣醋对慢性酒精肝损伤的保护作用[J].现代食品科技,2015,31(10):40-44.
[16]林晓晖,黄玲,王一铮,等.小鼠急性酒精性肝损伤模型的制备及观察[J].北方药学,2014,11(6):67-69.
[17]杨万枝,吕雄文.酒精性肝病发病机制研究进展[J].安徽医科大学报,2012,47(1):97-99.
[18]LU Y,CEDERBAUM A I.CYP2E1 and oxidative liver injury by alcohol[J].Free Radical Biology and Medicine,2008,44(5):723-738.
[19]CHENG D,KONG H.The effect of Lycium barbarum polysaccharide on alcohol-induced oxidative stress in rats[J].Molecules,2011,16(3):2542-2550.
[20]ALBANO E.Alcohol oxidative stress and free radical damage[J].Proceedings of the Nutrition Society,2006,65(3):278-290.