苜蓿黄酮的生物活性功能及其在反刍动物中的应用
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摘要
苜蓿作为畜禽青绿饲料,在动物生产中具有十分重要的价值和地位。其次级代谢产物黄酮类化合物具有杀菌、抗氧化、延缓衰老、抗肿瘤、类雌激素、增强机体免疫力等生物活性功能,在医药领域和畜牧生产中有着十分重要的开发与利用价值。本文系统综述了苜蓿黄酮生物活性功能和提取工艺方面的研究现状,分析造成苜蓿黄酮含量差异的因素,旨在探索加工生产功能性无抗饲料,为发展健康安全的畜产品提供理论参考依据。
Alfalfa,as livestock feed and forage grass,has important value and status in animal production.The flavonoids of alfalfa have biological activities such as bactericidal,anti-oxidation,anti-aging,anti-tumor,estrogen-like action and enhance immunity.They have important development and utilization value in the field of medicine and livestock production.In this paper,the research progress of the bioactive function and extraction process of flavonoids were reviewed.The factors causing the difference of flavonoids content were analyzed.The aim was to explore the theoretical basis for the production of functional non-anti-feed and the development of healthy and safe animal products.
Alfalfa,as livestock feed and forage grass,has important value and status in animal production.The flavonoids of alfalfa have biological activities such as bactericidal,anti-oxidation,anti-aging,anti-tumor,estrogen-like action and enhance immunity.They have important development and utilization value in the field of medicine and livestock production.In this paper,the research progress of the bioactive function and extraction process of flavonoids were reviewed.The factors causing the difference of flavonoids content were analyzed.The aim was to explore the theoretical basis for the production of functional non-anti-feed and the development of healthy and safe animal products.
引文
[1]董晓宁,赵海福,赵强,等.航天紫花苜蓿中黄酮的提取及抑菌活性[J].草业科学,2014,31(4):771~775.
[2]冯娜娜.不同光质对紫花苜蓿芽苗品质和抗氧化性影响的研究[D].新乡:河南师范大学,2016.
[3]高微微.苜蓿生物活性及影响其黄酮和皂甙成分因素的研究[D].北京:中国协和医科大学,2004.
[4]黄晓慧,张宏,李鹏,等.微波辅助提取航天紫花黄酮中总黄酮工艺研究[J].中国奶牛,2017,9:61~64.
[5]刘香萍,王国庆,李国良,等.响应面法优化提取紫花苜蓿中总黄酮及其抗氧化活性研究[J].中国食品学报,2016,16(4):145~152.
[6]刘艳丰,王梦竹,王文奇,等.苜蓿黄酮对绵羊血液抗氧化性能的影响[J].饲料工业,2016,37(1):50~52.
[7]唐浩国,魏晓霞,李叶,等.竹叶黄酮对小鼠脾细胞免疫的分子机制研究[J].食品科学,2007,9:523~526.
[8]王凯,王洋,孙娟娟,等.苜蓿和红车轴草黄酮提取物对绵羊生长性能和血液相关指标的影响[J].中国兽医学报,2017,37(4):704~709.
[9]王梦竹,刘艳丰,王文奇,等.苜蓿黄酮对绵羊生长性能和血清指标的影响[J].中国畜牧兽医,2015,42(9):2345~2351.
[10]王伟,王凤云,田苗苗,等.苜蓿总黄酮对未达性成熟期及成年雌性大鼠下丘脑-垂体-卵巢性腺轴相关表达量的影响[J].草地学报,2014,22(3):600~604.
[11]王艳芳,王新华,朱宇同.槲皮素药理作用研究进展[J].天然产物研究与开发,2003,15(2):171~173.
[12]许英一,王宇,杨伟光.紫花苜蓿叶总黄酮提取及抗氧化性[J].草地学报,2018,3:757~763.
[13]杨彩霞,田春莲,耿健,等.黄酮类化合物抗菌作用及机制的研究进展[J].中国畜牧兽医,2014,41(9):158~162.
[14]尹卫,杨国柱,张承胜,等.“青大1号”紫花苜蓿黄酮类物质提取方法优化[J].北方园艺,2016,4:125~127.
[15]占今舜,陈小连,詹康,等.苜蓿黄酮对脂多糖诱导下奶牛乳腺上皮细胞凋亡的影响[J].草业学报,2018,27(1):187~194.
[16]张羽男,黄淼,张宇,等.紫花苜蓿总黄酮的微波辅助提取及其体外抗肿瘤活性研究[J].时珍国医国药,2014,25(6):1300~1302.
[17]赵娅敏,马好文,孟淑娟.紫花苜蓿中黄酮类化学成分及其生物活性研究进展[J].中草药,2013,44(22):3245~3252.
[18]左晓昕,赵倩明,隋雁南,等.紫花苜蓿黄酮对人胃癌MGC-803细胞周期和凋亡的影响[J].时珍国医国药,2016,27(9):2104~2106.
[19]Babii C,Mihalache G,Bahrin L G,et al.A novel synthetic flavonoid with potent antibacterial properties:In vitro activity and proposed mode of action[J].PloS One,2018,13(4):e0194898.
[20]Barros L,Ferreira MJ,Queiros B,et al.Total phenols,ascorbic acid,β-carotene and lycopene in portuguese wild edible mushrooms and their antioxidant activities[J].Food Chemistry,2007,103(2):413~419.
[21]Birari R B,Bhutani KK.Pancreatic lipase inhibitors from natural sources:unexplored potential[J].Drug Discovery Today,2007,12(19~20):879~889.
[22]Colla G,Rouphael Y,Cardarelli M,et al.Effects of saline stress on mineral composition,phenolic acids and flavonoids in leaves of artichoke and cardoon genotypes grown in floating system[J].Journal of the Science of Food and Agriculture,2013,93(5):1119~1127.
[23]Collins-Burow B M,Antoon J W,Frigo D E,et al.Antiestrogenic activity of flavonoid phytochemicals mediated via the c-Jun N-terminal protein kinase pathway.Cell-type specific regulation of estrogen receptor alpha[J].Journal of Steroid Biochemistry and Molecular Biology,2012,132(1~2):186~193.
[24]Cornara L,Xiao J,Burlando B.Therapeutic potential of temperate forage legumes:a review[J].Critical Reviews in Food Science and Nutrition,2016,56 Suppl 1(Suppl 1):S149~161.
[25]Gatouillat G,Magid AA,Bertin E,et al.Cytotoxicity and apoptosis induced by alfalfa(Medicago sativa)leaf extracts in sensitive and multidrug-resistant tumor cells[J].Nutrition&Cancer-an International Journal,2014,66(3):483~491.
[26]Gawel E,Grzelak M,Janyszek M.Lucerne(MedicagosativaL.)in the human diet-Case reports and short reports[J].Journal of Herbal Medicine,2017,10:8~16.
[27]Gonzales GB,Smagghe G,Grootaert C,et al.Flavonoid interactions during digestion,absorption,distribution and metabolism:a sequential structure-activity/property relationship-based approach in the study of bioavailability and bioactivity[J].Drug Metabolism Reviews,2015,47(2):175~190.
[28]Gupta B,Huang B.Mechanism of salinity tolerance in plants:Physiological,biochemical,and molecular characterization[J].International Journal of Genomics,2014,2014(1):701596.
[29]Jing C L,Dong X F,Tong J M.Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method[J].Molecules,2015,20(9):15550~15571.
[30]Kang Y,Han Y,Torres-Jerez I,et al.System responses to longterm drought and re-watering of two contrasting alfalfa varieties[J].Plant Journal,2011,68(5):871~889.
[31]Katarzyna Rafińska,Pawel Pomastowski,Olga Wrona,et al.Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry[J].Phytochemistry Letters,2016,20:520~539.
[32]Karimi E,Oskoueian E,Oskoueian A,et al.Insight into the functional and medicinal properties of Medicago sativa(Alfalfa)leaves extract[J].Journal of the American Ceramic Society,2013,87(11):2093~2100.
[33]Liu X G,Huang M Y,Gao P Y,et al.Bioactive constituents from Medicago sativa L.with antioxidant,neuroprotective and acetylcholinesterase inhibitory activities[J].Journal of Functional Foods,2018,45:371~380.
[34]Martínez R,Kapravelou G,Porres J M,et al.Medicago sativa L.,a functional food to relieve hypertension and metabolic disorders in a spontaneously hypertensive rat model[J].Journal of Functional Foods,2016,26:470~484.
[35]Oskoueian E,Abdullah N,Hendra R,et al.Bioactive compounds,antioxidant,xanthine oxidase inhibitory,tyrosinase inhibitory and anti-Inflammatory activities of selected agro-Industrial by-products[J].International Journal of Molecular Sciences,2011,12(12):8610~8625.
[36]Pandey K B,Rizvi S I.Plant polyphenols as dietary antioxidants in human health and disease[J].Oxidative Medicine&Cellular Longevity,2009,2(5):270~278.
[37]Rodrigues F,Palmeiradeoliveira A,Das N J,et al.Medicago spp.extracts as promising ingredients for skin care products[J].Industrial Crops and Products,2013,49(4):634~644.
[38]Xu Y,Li L Z,Cong Q,et al.Bioactive lignans and flavones with in vitro antioxidant and neuroprotective properties from rubusidaeus rhizome[J].Journal of Functional Foods,2017,32:160~169.
[39]Zhan J,Liu M,Su X,et al.Effects of alfalfa flavonoids on the production performance,immune system,and ruminal fermentation of dairy cows[J].Asian-Australasian Journal of Animal Sciences,2017,30(10):1416~1424.
[2]冯娜娜.不同光质对紫花苜蓿芽苗品质和抗氧化性影响的研究[D].新乡:河南师范大学,2016.
[3]高微微.苜蓿生物活性及影响其黄酮和皂甙成分因素的研究[D].北京:中国协和医科大学,2004.
[4]黄晓慧,张宏,李鹏,等.微波辅助提取航天紫花黄酮中总黄酮工艺研究[J].中国奶牛,2017,9:61~64.
[5]刘香萍,王国庆,李国良,等.响应面法优化提取紫花苜蓿中总黄酮及其抗氧化活性研究[J].中国食品学报,2016,16(4):145~152.
[6]刘艳丰,王梦竹,王文奇,等.苜蓿黄酮对绵羊血液抗氧化性能的影响[J].饲料工业,2016,37(1):50~52.
[7]唐浩国,魏晓霞,李叶,等.竹叶黄酮对小鼠脾细胞免疫的分子机制研究[J].食品科学,2007,9:523~526.
[8]王凯,王洋,孙娟娟,等.苜蓿和红车轴草黄酮提取物对绵羊生长性能和血液相关指标的影响[J].中国兽医学报,2017,37(4):704~709.
[9]王梦竹,刘艳丰,王文奇,等.苜蓿黄酮对绵羊生长性能和血清指标的影响[J].中国畜牧兽医,2015,42(9):2345~2351.
[10]王伟,王凤云,田苗苗,等.苜蓿总黄酮对未达性成熟期及成年雌性大鼠下丘脑-垂体-卵巢性腺轴相关表达量的影响[J].草地学报,2014,22(3):600~604.
[11]王艳芳,王新华,朱宇同.槲皮素药理作用研究进展[J].天然产物研究与开发,2003,15(2):171~173.
[12]许英一,王宇,杨伟光.紫花苜蓿叶总黄酮提取及抗氧化性[J].草地学报,2018,3:757~763.
[13]杨彩霞,田春莲,耿健,等.黄酮类化合物抗菌作用及机制的研究进展[J].中国畜牧兽医,2014,41(9):158~162.
[14]尹卫,杨国柱,张承胜,等.“青大1号”紫花苜蓿黄酮类物质提取方法优化[J].北方园艺,2016,4:125~127.
[15]占今舜,陈小连,詹康,等.苜蓿黄酮对脂多糖诱导下奶牛乳腺上皮细胞凋亡的影响[J].草业学报,2018,27(1):187~194.
[16]张羽男,黄淼,张宇,等.紫花苜蓿总黄酮的微波辅助提取及其体外抗肿瘤活性研究[J].时珍国医国药,2014,25(6):1300~1302.
[17]赵娅敏,马好文,孟淑娟.紫花苜蓿中黄酮类化学成分及其生物活性研究进展[J].中草药,2013,44(22):3245~3252.
[18]左晓昕,赵倩明,隋雁南,等.紫花苜蓿黄酮对人胃癌MGC-803细胞周期和凋亡的影响[J].时珍国医国药,2016,27(9):2104~2106.
[19]Babii C,Mihalache G,Bahrin L G,et al.A novel synthetic flavonoid with potent antibacterial properties:In vitro activity and proposed mode of action[J].PloS One,2018,13(4):e0194898.
[20]Barros L,Ferreira MJ,Queiros B,et al.Total phenols,ascorbic acid,β-carotene and lycopene in portuguese wild edible mushrooms and their antioxidant activities[J].Food Chemistry,2007,103(2):413~419.
[21]Birari R B,Bhutani KK.Pancreatic lipase inhibitors from natural sources:unexplored potential[J].Drug Discovery Today,2007,12(19~20):879~889.
[22]Colla G,Rouphael Y,Cardarelli M,et al.Effects of saline stress on mineral composition,phenolic acids and flavonoids in leaves of artichoke and cardoon genotypes grown in floating system[J].Journal of the Science of Food and Agriculture,2013,93(5):1119~1127.
[23]Collins-Burow B M,Antoon J W,Frigo D E,et al.Antiestrogenic activity of flavonoid phytochemicals mediated via the c-Jun N-terminal protein kinase pathway.Cell-type specific regulation of estrogen receptor alpha[J].Journal of Steroid Biochemistry and Molecular Biology,2012,132(1~2):186~193.
[24]Cornara L,Xiao J,Burlando B.Therapeutic potential of temperate forage legumes:a review[J].Critical Reviews in Food Science and Nutrition,2016,56 Suppl 1(Suppl 1):S149~161.
[25]Gatouillat G,Magid AA,Bertin E,et al.Cytotoxicity and apoptosis induced by alfalfa(Medicago sativa)leaf extracts in sensitive and multidrug-resistant tumor cells[J].Nutrition&Cancer-an International Journal,2014,66(3):483~491.
[26]Gawel E,Grzelak M,Janyszek M.Lucerne(MedicagosativaL.)in the human diet-Case reports and short reports[J].Journal of Herbal Medicine,2017,10:8~16.
[27]Gonzales GB,Smagghe G,Grootaert C,et al.Flavonoid interactions during digestion,absorption,distribution and metabolism:a sequential structure-activity/property relationship-based approach in the study of bioavailability and bioactivity[J].Drug Metabolism Reviews,2015,47(2):175~190.
[28]Gupta B,Huang B.Mechanism of salinity tolerance in plants:Physiological,biochemical,and molecular characterization[J].International Journal of Genomics,2014,2014(1):701596.
[29]Jing C L,Dong X F,Tong J M.Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method[J].Molecules,2015,20(9):15550~15571.
[30]Kang Y,Han Y,Torres-Jerez I,et al.System responses to longterm drought and re-watering of two contrasting alfalfa varieties[J].Plant Journal,2011,68(5):871~889.
[31]Katarzyna Rafińska,Pawel Pomastowski,Olga Wrona,et al.Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry[J].Phytochemistry Letters,2016,20:520~539.
[32]Karimi E,Oskoueian E,Oskoueian A,et al.Insight into the functional and medicinal properties of Medicago sativa(Alfalfa)leaves extract[J].Journal of the American Ceramic Society,2013,87(11):2093~2100.
[33]Liu X G,Huang M Y,Gao P Y,et al.Bioactive constituents from Medicago sativa L.with antioxidant,neuroprotective and acetylcholinesterase inhibitory activities[J].Journal of Functional Foods,2018,45:371~380.
[34]Martínez R,Kapravelou G,Porres J M,et al.Medicago sativa L.,a functional food to relieve hypertension and metabolic disorders in a spontaneously hypertensive rat model[J].Journal of Functional Foods,2016,26:470~484.
[35]Oskoueian E,Abdullah N,Hendra R,et al.Bioactive compounds,antioxidant,xanthine oxidase inhibitory,tyrosinase inhibitory and anti-Inflammatory activities of selected agro-Industrial by-products[J].International Journal of Molecular Sciences,2011,12(12):8610~8625.
[36]Pandey K B,Rizvi S I.Plant polyphenols as dietary antioxidants in human health and disease[J].Oxidative Medicine&Cellular Longevity,2009,2(5):270~278.
[37]Rodrigues F,Palmeiradeoliveira A,Das N J,et al.Medicago spp.extracts as promising ingredients for skin care products[J].Industrial Crops and Products,2013,49(4):634~644.
[38]Xu Y,Li L Z,Cong Q,et al.Bioactive lignans and flavones with in vitro antioxidant and neuroprotective properties from rubusidaeus rhizome[J].Journal of Functional Foods,2017,32:160~169.
[39]Zhan J,Liu M,Su X,et al.Effects of alfalfa flavonoids on the production performance,immune system,and ruminal fermentation of dairy cows[J].Asian-Australasian Journal of Animal Sciences,2017,30(10):1416~1424.