扬麦17/宁麦18的F_2群体株高QTL定位及分析
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摘要
扬麦17和宁麦18均为高产小麦品种,为研究两者的株高遗传机制,以扬麦17与宁麦18杂交获得的310个F_2单株及其衍生F_(2:3)家系为材料,利用已构建的覆盖19条染色体的遗传连锁图谱,对株高性状进行QTL定位。应用复合区间作图法定位到8个株高QTL,分别位于2D、4B、5A、5B和7A染色体上,4个QTL在两世代中均检测到,其中 QPh-YN-5A.1为一主效株高QTL位点,矮秆效应来自扬麦17,LOD值为8.69和12.81,可解释14.36%和20.46%的表型变异; QPh-YN-2D.1、 QPh-YN-5B.2和 QPh-YN-7A的矮秆效应来自宁麦18,可在两世代中分别解释2.84%和6.37%、4.26%和5.46%、2.15%和3.16%的表型变异。另外4个QTL仅在单世代检测到,其中 QPh-YN-2D.2、 QPh-YN-4B和 QPh-YN-5B.1的矮秆效应来自宁麦18,可分别解释5.05%、11.24%和4.62%的表型变异, QPh-YN-5A.2的矮秆效应来自扬麦17,可解释7.72%的表型变异。
Both Yangmai 17 and Ningmai 18 are high-yielding wheat varieties. To study the genetic mechanism of plant height,the population of 310 F_2 and its F_(2:3) generations derived from the hybridization between Yangmai 17 and Ningmai 18 were used to detect the quantitative trait loci(QTLs) for plant height. A genetic linkage map covering 19 chromosomes of wheat was established,and QTL mapping of plant height traits was carried out. A total of eight plant height QTLs were mapped by Composite Interval Mapping(CIM) based on the genetic map and phenotypic data,which are located on 2 D,4 B,5 A,5 B and 7 A chromosomes,and four QTLs were detected in both generations. QPh-YN-5 A.1 is a main-effect QTL,explaining 14.36% and 20.46% of the phenotypic variances,the dwarfing effect of which was contributed by Yangmai 17. QPh-YN-2 D.1, QPh-YN-5 B.2 and QPh-YN-7 A explained 2.84% and 6.37%,4.26% and 5.46%,2.15% and 3.16% of the phenotypic variances respectively,the dwarfing effect of which was contributed by Ningmai 18. Four QTLs were detected only in a single generation. QPh-YN-2 D.2, QPh-YN-4 B and QPh-YN-5 B.1 explained 5.05%,11.24% and 4.62% of the phenotypic variances respectively,the dwarfing effect of which was contributed by Ningmai 18. QPh-YN-5 A.2 explained 7.72% of the phenotypic variances,the dwarfing effect of which was contributed by Yangmai 17.
Both Yangmai 17 and Ningmai 18 are high-yielding wheat varieties. To study the genetic mechanism of plant height,the population of 310 F_2 and its F_(2:3) generations derived from the hybridization between Yangmai 17 and Ningmai 18 were used to detect the quantitative trait loci(QTLs) for plant height. A genetic linkage map covering 19 chromosomes of wheat was established,and QTL mapping of plant height traits was carried out. A total of eight plant height QTLs were mapped by Composite Interval Mapping(CIM) based on the genetic map and phenotypic data,which are located on 2 D,4 B,5 A,5 B and 7 A chromosomes,and four QTLs were detected in both generations. QPh-YN-5 A.1 is a main-effect QTL,explaining 14.36% and 20.46% of the phenotypic variances,the dwarfing effect of which was contributed by Yangmai 17. QPh-YN-2 D.1, QPh-YN-5 B.2 and QPh-YN-7 A explained 2.84% and 6.37%,4.26% and 5.46%,2.15% and 3.16% of the phenotypic variances respectively,the dwarfing effect of which was contributed by Ningmai 18. Four QTLs were detected only in a single generation. QPh-YN-2 D.2, QPh-YN-4 B and QPh-YN-5 B.1 explained 5.05%,11.24% and 4.62% of the phenotypic variances respectively,the dwarfing effect of which was contributed by Ningmai 18. QPh-YN-5 A.2 explained 7.72% of the phenotypic variances,the dwarfing effect of which was contributed by Yangmai 17.
引文
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[22]KORAUN V,RODER M,WORLAND A J,et al.Intrachromosomal mapping of genes for dwarfing( Rht12) and vernalization response( Vrn1) in wheat by using RFLP and microsatellite markers [J].Plant Breeding,1997,116(3):228.
[23]WANG S C,WONG D,FORREST K,et al.Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array [J].Plant Biotechnology Journal,2014,12:796.
[24]GAO F M,WEN W E,LIU J D,et al.Genome-wide linkage mapping of QTL for yield components,plant height and yield-related physiological traits in the Chinese wheat cross Zhou 8425B/Chinese Spring [J].Frontiers in Plant Science,2015(6):13.
[25]KORZUM V,RODER M S,GANAL M W,et al.Genetic analysis ofthe dwarfing gene( Rht8) in wheat.Part I.Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat(Triticum aestivum L.) [J].Theoretical and Applied Genetics,1998,96:1107.
[26]叶亚琼,栗孟飞,刘媛,等.小麦株高QTL定位及其水分环境互作遗传分析 [J].华北农学报,2015,30(5):86.YE Y Q,LI M F,LIU Y,et al.QTL mapping and QTL×Environmental interactions for plant height in wheat [J].Acta Agriculturae Boreali-Occidentalis Sinica,2015,30(5):86.
[27]陈广凤,陈建省,田纪春.小麦株高相关性状与SNP标记全基因组关联分析 [J].作物学报,2015,41(10):1504.CHEN G F,CHEN J X,TIAN J C.Genome-wide association analysis between SNP markers and plant height related traits in wheat [J].Acta Agronomi Casinica,2015,41(10):1504.
[28]李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答 [J].作物学报,2010,36(6):929.LI H H,ZHANG L Y,WANG J K.Analysis and answers to frequently asked questions in quantitative trait locus mapping [J].Acta Agronomica Sinica,2010,36(6):929.
[2]ELLIS M H,SPIELMEYER W,GALE K R,et al.“Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat [J].Theoretical and Applied Genetics,2002,105:1038.
[3]唐娜,李博,闵红,等.分子标记检测矮秆基因 Rht-B1b, Rht-D1b和 Rht8在我国小麦中的分布 [J].中国农业大学学报,2012,17(4):24.TANG N,LI B,MIN H,et al.Distribution of dwarfing genes Rht-B1b, Rht-D1b and Rht8 in Chinese bread wheat cultivars detected by molecular markers [J].Journal of China Agricultural University,2012,17(4):24.
[4]PEARCE S,SAVILLE R,VAUGHAN S P,et al.Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat [J].Plant Physiology,2011,157:1822.
[5]WU J,KONG X,WAN J,et al.Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1 [J].Plant Physiology,2011,157:2120.
[6]CHEN S L,GAO R H,WANG H Y,et al.Characterization of a novel reduced height gene(Rht23) regulating panicle morphology and plant architecture in bread wheat [J].Euphytica,2015,203:587.
[7]TIAN X L,WEN W E,XIE L,et al.Molecular mapping of reduced plant height gene Rht24 in bread wheat [J].Frontiers in Plant Science,2017,8:1039.
[8]WURSCHUM T,LANGER S M,LONGIN C F.Genetic control of plant height in European winter wheat cultivars [J].Theoretical and Applied Genetics,2015,128:865.
[9]唐娜,逯芳芳,何蓓如,等.矮秆基因对小麦部分农艺性状的效应 [J].西北植物学报,2010,30(1):46.TANG N,LU F F,HE B R,et al.Effects of dwarfing genes on some agronomic characteristics of wheat [J].Acta Botanica Boreali-Occidentalia Sinica,2010,30(1):46.
[10]LAW C N,SNAPE J W,WORLAND A J.Interspecific chromosome manipulation [J].Philosophical Transactions of the Royal Society of London(B),1981,292:509.
[11]余泽高,方燕妮.小麦经济系数与其它若干性状关系的研究 [J].湖北农学院学报,2002,22(6):487.YU Z G,FANG Y N.Study on the relationship between wheat economic coefficient and several other traits [J].Journal of Hubei Agricultural College,2002,22(6):487.
[12]陈亮.矮秆基因 Rht12对小麦重要农艺性状的遗传效应及新矮秆突变体的筛选[D].杨凌:西北农林科技大学,2014:12.CHEN L.Genetic effects of dwarfing gene Rht12 on important agronomic traits of wheat and screening of new dwarf mutants [D].Yangling:Northwest A&F University,2014:12.
[13]姚金保,马鸿翔,姚国才,等.高产抗病弱筋小麦新品种宁麦18的选育及栽培技术 [J].江苏农业科学,2012,40(10):91.YAO J B,MA H X,YAO G C,et al.Breeding and cultivation techniques of nwe wheat variety Ningmai 18 with high yield,disease resistance and weak gluten [J].Jiangsu Agricultural Science,2012,40(10):91.
[14]RODER M S,KORZUN V,Gill B S,et al.The physical mapping of microsatellite markers in wheat [J].Genome,1998,41:280.
[15]SONG Q J,FICKUS E W,CREGAN P B.Characterization of trinucleotide SSR motifs in wheat [J].Theoretial and Applied Genetics,2002,104:289.
[16]SOURDILE P,SINGH S W,CADALEN T,et al.Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat(Triticum aestivum L.) [J].Funct Integr Genomics,2004,4:21.
[17]GUYOMARC'H,SOURDILLE P,CHARMET G V,et al.Characterization of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D genome of bread wheat [J].Theoretial and Applied Genetics,2002,104:1170.
[18]LEI L,SUN F Y,WU D,et al.High-throughput development of genome-wide locus-specific informative SSR markers in wheat [J].Science China Life Sciences,2017,60(6):673.
[19]ZENG Z B.Precision mapping of quantitative trait loci [J].Genetics,1994,136(4):1457.
[20]MCCOUCH S R,CHO Y G,YANO M,et al.Report on QTL nomenclature [J].Rice Genet Newsl,1997,14:11.
[21]梁秀梅,胡文静,李东升,等.扬麦17/宁麦18 F2群体穗部性状的QTL定位 [J].麦类作物学报,2018,38(5):511.LIANG X M,HU W J,LI D S L,et al.QTL mapping for spike traits in wheat(Triticum aestivum L.) using F2 population of Yangmai 17/Ningmai 18[J].Journal of Triticeae Crops,2018,38(5):511.
[22]KORAUN V,RODER M,WORLAND A J,et al.Intrachromosomal mapping of genes for dwarfing( Rht12) and vernalization response( Vrn1) in wheat by using RFLP and microsatellite markers [J].Plant Breeding,1997,116(3):228.
[23]WANG S C,WONG D,FORREST K,et al.Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array [J].Plant Biotechnology Journal,2014,12:796.
[24]GAO F M,WEN W E,LIU J D,et al.Genome-wide linkage mapping of QTL for yield components,plant height and yield-related physiological traits in the Chinese wheat cross Zhou 8425B/Chinese Spring [J].Frontiers in Plant Science,2015(6):13.
[25]KORZUM V,RODER M S,GANAL M W,et al.Genetic analysis ofthe dwarfing gene( Rht8) in wheat.Part I.Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat(Triticum aestivum L.) [J].Theoretical and Applied Genetics,1998,96:1107.
[26]叶亚琼,栗孟飞,刘媛,等.小麦株高QTL定位及其水分环境互作遗传分析 [J].华北农学报,2015,30(5):86.YE Y Q,LI M F,LIU Y,et al.QTL mapping and QTL×Environmental interactions for plant height in wheat [J].Acta Agriculturae Boreali-Occidentalis Sinica,2015,30(5):86.
[27]陈广凤,陈建省,田纪春.小麦株高相关性状与SNP标记全基因组关联分析 [J].作物学报,2015,41(10):1504.CHEN G F,CHEN J X,TIAN J C.Genome-wide association analysis between SNP markers and plant height related traits in wheat [J].Acta Agronomi Casinica,2015,41(10):1504.
[28]李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答 [J].作物学报,2010,36(6):929.LI H H,ZHANG L Y,WANG J K.Analysis and answers to frequently asked questions in quantitative trait locus mapping [J].Acta Agronomica Sinica,2010,36(6):929.