宫炎平对子代雄性大鼠生殖发育的影响
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摘要
目的研究宫炎平对子代雄性大鼠生殖发育的影响。方法选取100只妊娠大鼠,随机分为溶媒对照组、低剂量组、中剂量组、高剂量组共4组。各组妊娠大鼠于妊娠15 d至离乳,每日给予雌性大鼠不同剂量的宫炎平片。子代雄性大鼠出生后正常喂养至出生后第43 d。测定子代雄性大鼠的体重,测量脏器系数,检测精子数、精子活动百分数等指标。结果中剂量组脾脏重量、脾脏系数高于溶媒对照组,存在显著性差异(P<0.05),低剂量组及高剂量组胸腺系数存在显著性差异(P<0.01),其余脏器重量及脏器重量系数统计未见异常;各剂量组雄性大鼠睾丸重、附睾重、睾丸重量系数、附睾重量系数无统计学意义(P>0.05)。检查精子活力及精子畸形情况,各剂量组与溶媒对照组比较均无统计学意义(P>0.05)。结论宫炎平对子代雄性大鼠生殖发育无毒性影响。
OBJECTIVE To study the effect of Gongyanping Tablets( GYP) on the reproductive development of offspring male rats. METHODS The respective GYP administration of 0,0.6,1.5,3.75 g·kg-1 to the four groups rats,each with twenty-four female rats,started from the day 15 of gestation( GD15) through weaning. The weight of offspring male rats were detected. Organ coefficient,sperm count,and the percentage of sperm motility were detected at PND43. RESULTS Compared with solvent control group,there was statistically significant difference in spleen weight and spleen coefficient of the GYP middle dose group( P < 0. 05). There was significant difference in the thymus coefficient of the low dose group and the high dose group( P<0.01).The remaining organ weight and organ weight coefficient statistics were normal. There was no significant difference in testicular weight,epididymis weight,testicular weight coefficient and epididymis weight coefficient on male rats in each dose group( P > 0. 05). There was no significant difference between the dose groups and the solvent control group in sperm motility and sperm deformity( P>0.05). CONCLUSION GYP has no toxic effect on reproductive development of offspring male rat.
OBJECTIVE To study the effect of Gongyanping Tablets( GYP) on the reproductive development of offspring male rats. METHODS The respective GYP administration of 0,0.6,1.5,3.75 g·kg-1 to the four groups rats,each with twenty-four female rats,started from the day 15 of gestation( GD15) through weaning. The weight of offspring male rats were detected. Organ coefficient,sperm count,and the percentage of sperm motility were detected at PND43. RESULTS Compared with solvent control group,there was statistically significant difference in spleen weight and spleen coefficient of the GYP middle dose group( P < 0. 05). There was significant difference in the thymus coefficient of the low dose group and the high dose group( P<0.01).The remaining organ weight and organ weight coefficient statistics were normal. There was no significant difference in testicular weight,epididymis weight,testicular weight coefficient and epididymis weight coefficient on male rats in each dose group( P > 0. 05). There was no significant difference between the dose groups and the solvent control group in sperm motility and sperm deformity( P>0.05). CONCLUSION GYP has no toxic effect on reproductive development of offspring male rat.
引文
[1]潘立行,谭永恒,肖细姬,等.宫炎平片体内、外抑菌作用研究[J].今日药学,2012,22(2):85-87.
[2]国家食品药品监督管理局.药物生殖毒性研究技术指导原则[Z].国食药监注[2006]639号.
[3]袁伯俊,廖明阳,李波.药物毒理学实验方法与技术[M].北京:化学工业出版社,2007:333.
[4]范玉明,张舒.毒理学安全性评价标准操作规程指南[M].成都:电子科技大学出版社,2009:369-370.
[5]孙祖越,周莉,闫晗,等.如何成功开展药物临床前生殖毒性试验[J].中国新药杂志2011,20(22):2195-2204.
[6]吴丽明,黄伯俊.多菌灵对小鼠精子形态及雄性生育力的影响[J].职业医学,1992,25(1):41-47.
[7]傅剑云,宋燕华,何佳维,等.铁皮石斛叶对亲代和子代雄性大鼠生殖系统的影响[J].预防医学,2017,29(8):782-785.
[8]吴红娟,佟茵,邱赛红,等.盐酸青藤碱对雄性大鼠精子质量及睾丸组织的影响[J].湖南中医药大学学报,2015,35(7):12-14,29.
[9]杨滨,夏欣一,黄宇烽,等.精子的表观遗传学研究进展[J].中华男科学杂志,2017,13(12):1125-1129.
[10]张莹,王尚明,张斌,等.慢性不可预知性温和刺激对雄性大鼠生殖系统的影响[J].中国计划生育学杂志,2015,23(7):440-442.
[11]高艳,王志新,常成,等.亚硝酸盐暴露致雄性小鼠生殖毒性的探讨[J].解剖学报,2017,48(5):576-584.
[12]张莹,王尚明,张斌,等.慢性不可预知性温和刺激对雄性大鼠生殖系统的影响[J].中国计划生育学杂志,2015,23(7):440-442.
[13]刘清珍,邵永,商学军,等.吗啡耐受对雄性大鼠生精功能影响的初步探讨[J].中华男科学杂志,2014,20(12):1073-1076.
[14]唐夏楠,杨涛,岳静,等.常用麻醉药对雄性生殖毒性的研究现状[J].中华生殖与避孕杂志,2018,38(8):698-700.
[15]潘立行,谭永恒,肖细姬,等.宫炎平片体内、外抑菌作用研究[J].今日药学,2012,22(2):85-87.
[16]张延林,孔卫青,樊勇,等.精子分析仪和血细胞计数板计数大鼠附睾精子的比较[J].中国药理学与毒理学杂志,2013,27(3):582.
[17]苏晓慧,孙明琪,张梅洁,等.穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用[J].山东大学学报(医学版),2018,56(6):1-5.
[18]史晓蓉,刘舒瑜,陈亚,等.芹菜素对小鼠精液参数影响的研究[J].中华男科学杂志,2010,16(9):778-782.
[2]国家食品药品监督管理局.药物生殖毒性研究技术指导原则[Z].国食药监注[2006]639号.
[3]袁伯俊,廖明阳,李波.药物毒理学实验方法与技术[M].北京:化学工业出版社,2007:333.
[4]范玉明,张舒.毒理学安全性评价标准操作规程指南[M].成都:电子科技大学出版社,2009:369-370.
[5]孙祖越,周莉,闫晗,等.如何成功开展药物临床前生殖毒性试验[J].中国新药杂志2011,20(22):2195-2204.
[6]吴丽明,黄伯俊.多菌灵对小鼠精子形态及雄性生育力的影响[J].职业医学,1992,25(1):41-47.
[7]傅剑云,宋燕华,何佳维,等.铁皮石斛叶对亲代和子代雄性大鼠生殖系统的影响[J].预防医学,2017,29(8):782-785.
[8]吴红娟,佟茵,邱赛红,等.盐酸青藤碱对雄性大鼠精子质量及睾丸组织的影响[J].湖南中医药大学学报,2015,35(7):12-14,29.
[9]杨滨,夏欣一,黄宇烽,等.精子的表观遗传学研究进展[J].中华男科学杂志,2017,13(12):1125-1129.
[10]张莹,王尚明,张斌,等.慢性不可预知性温和刺激对雄性大鼠生殖系统的影响[J].中国计划生育学杂志,2015,23(7):440-442.
[11]高艳,王志新,常成,等.亚硝酸盐暴露致雄性小鼠生殖毒性的探讨[J].解剖学报,2017,48(5):576-584.
[12]张莹,王尚明,张斌,等.慢性不可预知性温和刺激对雄性大鼠生殖系统的影响[J].中国计划生育学杂志,2015,23(7):440-442.
[13]刘清珍,邵永,商学军,等.吗啡耐受对雄性大鼠生精功能影响的初步探讨[J].中华男科学杂志,2014,20(12):1073-1076.
[14]唐夏楠,杨涛,岳静,等.常用麻醉药对雄性生殖毒性的研究现状[J].中华生殖与避孕杂志,2018,38(8):698-700.
[15]潘立行,谭永恒,肖细姬,等.宫炎平片体内、外抑菌作用研究[J].今日药学,2012,22(2):85-87.
[16]张延林,孔卫青,樊勇,等.精子分析仪和血细胞计数板计数大鼠附睾精子的比较[J].中国药理学与毒理学杂志,2013,27(3):582.
[17]苏晓慧,孙明琪,张梅洁,等.穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用[J].山东大学学报(医学版),2018,56(6):1-5.
[18]史晓蓉,刘舒瑜,陈亚,等.芹菜素对小鼠精液参数影响的研究[J].中华男科学杂志,2010,16(9):778-782.