Box-Behnken响应面法优化茜草中大叶茜草素提取工艺
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摘要
目的:基于Box-Behnken响应面法优化茜草中大叶茜草素的提取工艺。方法:以大叶茜草素的含量为响应值,以提取时间、乙醇体积分数、料液比、提取次数为考察因素,进行茜草中大叶茜草素的提取工艺优化试验。结果:经响应面分析,得最佳提取工艺为乙醇体积分数82%,提取时间97 min,料液比1∶10,提取2次,在此条件下提取大叶茜草素含量可达2.39%,接近预测值2.41%。结论:该优化工艺简便稳定,重复性好,可用于茜草中大叶茜草素的提取。
Objective:To optimize the extraction process of mollugin in Rubia cordifolia L. by the BoxBehnken response surface method. Methods:Taking the content of mollugin as response value,taking extraction time,ethanol volume fraction,material liquid ratio and extraction times as research factors,we optimized the extraction process of mollugin in Rubia cordifolia L.. Results:The optimum extraction conditions were as follows:the extraction time was 97 min,ethanol volume fraction was 82% and materialliquid ratio was 1 ∶ 10 m L. Under these conditions,the content of mollugin was 2.39%,which was well matched with the predicted content of 2.41%. Conclusion:This optimal extraction process can be used for extracting the mollugin in Rubia cordifolia L.. It is simple,stable and reproducible.
Objective:To optimize the extraction process of mollugin in Rubia cordifolia L. by the BoxBehnken response surface method. Methods:Taking the content of mollugin as response value,taking extraction time,ethanol volume fraction,material liquid ratio and extraction times as research factors,we optimized the extraction process of mollugin in Rubia cordifolia L.. Results:The optimum extraction conditions were as follows:the extraction time was 97 min,ethanol volume fraction was 82% and materialliquid ratio was 1 ∶ 10 m L. Under these conditions,the content of mollugin was 2.39%,which was well matched with the predicted content of 2.41%. Conclusion:This optimal extraction process can be used for extracting the mollugin in Rubia cordifolia L.. It is simple,stable and reproducible.
引文
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[2]陈毅,王海丽,薛露,等.茜草的研究进展[J].中草药,2017,48(13):2771-2779.
[3]欧莉,彭成,高晓庆,等.茜草治疗功能性子宫出血的药效学研究[J].中医药导报,2015,21(13):49-51.
[4]朱正光.大叶茜草素抑制脂多糖诱发的炎症反应及分子机制的实验研究[C]//中国药理学会抗炎免疫药理专业委员会.第十届全国抗炎免疫药理学学术会议论文集.西宁:中国药理学会抗炎免疫药理专业委员会,2010:1.
[5]李俊峰,舒建昌,杨钦河,等.大叶茜草素抑制大鼠CFSC-2G细胞活化和胶原合成[J].中国病理生理杂志,2017,33(12):2259-2263.
[6]王晓建,黄胜阳.茜草属植物化学成分及其药理作用研究进展[J].中国中医药信息杂志,2012,19(2):109-112.
[7]康文艺,郅妙利,王金梅,等.RP-HPLC法测定茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-Lrhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量[J].天然产物研究与开发,2008(2):295-297.
[8]许兰芝,陈维宁,张薇,等.茜草醇提物的抗炎免疫作用[J].潍坊医学院学报,2002(1):1-3.
[9]耿天华,汪涛,郭巧生,等.响应面法优化大孔树脂纯化昆仑雪菊色素的工艺[J].中国中药杂志,2016,41(2):220-225.
[10]赵呈雷,曹鹏.响应面法优化葛根素微丸片的处方及工艺[J].中国新药杂志,2018(1):84-90.
[11]张南生,郭虹,孙卫军,等.Box-Behnken试验设计法优化人参皂苷Rg_3自乳化释药系统和质量评价[J].中草药,2015,46(23):3500-3506.
[12]王双双,刘睿,何新.星点设计-效应面法优化桔梗泡腾片的处方[J].中国实验方剂学杂志,2016,22(12):23-27.
[13]苏珊珊,陈文,方贺,等.Box-Behnken效应面法优化松果菊苷PLGA纳米粒处方工艺[J].中国新药杂志,2016,25(9):1069-1074.