复方紫草油体外抑菌实验研究
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摘要
目的:研究复方紫草油主要成分及成药的体外抑菌作用特点。方法:利用平板打孔法、牛津杯法和菌落计数法研究1)紫草、白芷、忍冬藤水煎液及不同浓度醇提液对金黄色葡萄球菌的抑制能力; 2)紫草、白芷的95%乙醇浸泡液及忍冬藤水煎液对创面常见感染菌种和益生菌的抑制作用; 3)含50%复方紫草油的复方紫草油-乳化剂混合物对创面常见感染菌种和益生菌的抑制作用。结果:复方紫草油的抑菌特点与紫草醇提物抑菌特点基本一致,对白色念珠菌、铜绿假单胞菌、金黄色葡萄球菌、表皮葡萄球菌等常见感染致病菌的ATCC菌株均有较好抑制作用,同时也对益生菌有一定抑制作用,对大肠埃希菌无效。结论:复方紫草油抑菌谱不等于各组分抑菌谱的简单叠加。根据该药抑菌特点,可以考虑用于烧烫伤、特应性皮炎等病的创面护理。
Objective: To study the characteristics of antibacterial activity in vitro of the main components and compound medicines of compound Radix Lithospermi oil. Methods: The plate punching method,oxford cup method and colony counting method were used to study 1) the inhibitory ability of Radix Lithospermi,Radix Angelicae Dahuricae,Caulis Lonicerae Decoction and different concentrations of alcohol extract on staphylococcus aureus; 2) inhibition of common infectious strains and probiotics on wounds by95% ethanol soaking solution of Radix Lithospermi and Radix Angelicae Dahuricae; 3) inhibition of common infectious strains and probiotics on wounds by compound Radix Lithospermi oil-emulsifier mixture containing 50% compound Radix Lithospermi oil. Results: The antibacterial characteristics of compound Radix Lithospermi oil were basically consistent with the antibacterial characteristics of licorice extract,and the ATCC strains of common pathogens such as candida albicans,pseudomonas aeruginosa,staphylococcus aureus,staphylococcus epidermidis had good inhibitory effect,but also had a certain inhibitory effect on probiotics,and was ineffective against Escherichia coli. Conclusion: The antibacterial spectrum of compound Radix Lithospermi oil is not equal to the simple superposition of the antibacterial spectrum of each component. According to the antibacterial characteristics of this medicine,wound care for burns,atopic dermatitis and other diseases can be considered.
Objective: To study the characteristics of antibacterial activity in vitro of the main components and compound medicines of compound Radix Lithospermi oil. Methods: The plate punching method,oxford cup method and colony counting method were used to study 1) the inhibitory ability of Radix Lithospermi,Radix Angelicae Dahuricae,Caulis Lonicerae Decoction and different concentrations of alcohol extract on staphylococcus aureus; 2) inhibition of common infectious strains and probiotics on wounds by95% ethanol soaking solution of Radix Lithospermi and Radix Angelicae Dahuricae; 3) inhibition of common infectious strains and probiotics on wounds by compound Radix Lithospermi oil-emulsifier mixture containing 50% compound Radix Lithospermi oil. Results: The antibacterial characteristics of compound Radix Lithospermi oil were basically consistent with the antibacterial characteristics of licorice extract,and the ATCC strains of common pathogens such as candida albicans,pseudomonas aeruginosa,staphylococcus aureus,staphylococcus epidermidis had good inhibitory effect,but also had a certain inhibitory effect on probiotics,and was ineffective against Escherichia coli. Conclusion: The antibacterial spectrum of compound Radix Lithospermi oil is not equal to the simple superposition of the antibacterial spectrum of each component. According to the antibacterial characteristics of this medicine,wound care for burns,atopic dermatitis and other diseases can be considered.
引文
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[12]杨璐,吴长梦,李庆蓉,等.2073株烧伤患者创面分泌物病原菌种类分布及耐药性分析[J].中国抗生素杂志,2018,43(5):577-582.
[13]朱红,宋莉莉.婴儿尿布皮炎与念珠菌感染的关系[J].浙江医学,2002,24(3):169-170.
[2]陈书杨,高兴新,梁自乾,等.小儿烧伤患者病原菌分布及耐药性分析[J].现代预防医学,2016,43(6):1027-1029,1036.
[3]文细毛,任南,吴安华,等.全国医院感染监控网老年患者医院感染病原菌及其耐药特征分析[J].中华医院感染学杂志,2005,15(12):1346-1348.
[4]丁华荣,胡加平,李德绘,等.烧伤整形外科1963株细菌及其耐药情况分析[J].广西医科大学学报,2019,36(1):102-106.
[5]王源,赵文文,刁新平.枯草芽孢杆菌的研究进展[J].黑龙江畜牧兽医,2018,4(19):62-64.
[6]李晓云,牟阳,王淑清.枯草芽孢杆菌活菌制剂促进创伤皮肤愈合的实验研究[J].黑龙江医药,2002,15(5):369-370.
[7]高媛,刘云云,马晓昱,等.复方紫草油微生物限度检查法的建立[J].实用医药杂志,2018,35(1):51-53.
[8]刘小萍,高延瑞.抗菌肽和微生态在银屑病中的研究进展[J].中国麻风皮肤病杂志,2013,29(11):712-714.
[9]樊春梅,张虎田.新辅助化疗治疗晚期卵巢癌的疗效观察[J].中国继续医学教育,2016,8(33):195-197.
[10]Kong HH,Oh J,Deming C,et al.Temporal shifts in the skin microbiome associated with disease flares and treatment in children with atopic dermatitis[J].Genome Res,2012,22(5):850-859.
[11]张秀钦.特应性皮炎患者皮损致病真菌定植分析[D].福州:福建医科大学,2013.
[12]杨璐,吴长梦,李庆蓉,等.2073株烧伤患者创面分泌物病原菌种类分布及耐药性分析[J].中国抗生素杂志,2018,43(5):577-582.
[13]朱红,宋莉莉.婴儿尿布皮炎与念珠菌感染的关系[J].浙江医学,2002,24(3):169-170.