香蕉假茎生物炭对根际土壤细菌丰度和群落结构的影响
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摘要
【目的】将香蕉假茎生物炭施加到土壤,探讨香蕉假茎生物炭对香蕉根际土壤微生物的影响。【方法】以香蕉假茎生物炭(BPB)0、1%、2%、3%的质量比与土壤均匀混合。盆栽培养3个月后分离香蕉根际土壤。采用16S rRNA高通量测序技术对根际土壤细菌群落结构和丰度进行表征。【结果】提高BPB施用量可增加土壤有机质、有效钾、有效磷含量,提高土壤pH值,但降低有效氮浓度。在1%BPB样品中获得2278个OTUs,其显示细菌群落中的最大多样性。施加3%的BPB处理土壤,拟杆菌门、疣微菌门和厚壁菌门的相对丰度显著增加;放线菌门、酸杆菌门、芽单胞菌门明显减少。主成分分析发现,1%BPB和2%BPB处理的样本之间土壤细菌群落相似。【结论】施加不同比例BPB改变了根际土壤中细菌丰度和群落结构,且高比例添加改变更加明显。
[Objective] To study the effect of banana pseudostem biochar on soil microorganisms in banana rhizosphere, we added the biochar into soil. [Methods] We mixed banana pseudostem biochar(BPB) with soil uniformly at the mass ratio of 0, 1%, 2% and 3%. Banana rhizosphere soil was separated after 3 months of pot culture. The bacterial community structure and abundance in rhizosphere soil were characterized by 16 S rRNA high throughput sequencing. [Results] Increasing BPB application could increase soil organic matter, available potassium, available phosphorus contents and increase soil pH value, but decrease available nitrogen concentration.In total 2278 OTUs were obtained from 1% BPB samples, showing the greatest diversity of bacterial communities.When 3% BPB was applied to the soil, the relative abundance of Bacteroidates, Verrucomicrobia and Firmicutes increased significantly, whereas that of Actinobacteria, Acidobacteria and Gemmatimonade decreased significantly.Principal component analysis showed that soil bacterial communities were similar between 1% BPB and 2% BPB treated samples. [Conclusion] We changed the bacterial abundance and community structure in rhizosphere soil by applying different proportion of BPB, and the change in high proportion of BPB was more obvious.
[Objective] To study the effect of banana pseudostem biochar on soil microorganisms in banana rhizosphere, we added the biochar into soil. [Methods] We mixed banana pseudostem biochar(BPB) with soil uniformly at the mass ratio of 0, 1%, 2% and 3%. Banana rhizosphere soil was separated after 3 months of pot culture. The bacterial community structure and abundance in rhizosphere soil were characterized by 16 S rRNA high throughput sequencing. [Results] Increasing BPB application could increase soil organic matter, available potassium, available phosphorus contents and increase soil pH value, but decrease available nitrogen concentration.In total 2278 OTUs were obtained from 1% BPB samples, showing the greatest diversity of bacterial communities.When 3% BPB was applied to the soil, the relative abundance of Bacteroidates, Verrucomicrobia and Firmicutes increased significantly, whereas that of Actinobacteria, Acidobacteria and Gemmatimonade decreased significantly.Principal component analysis showed that soil bacterial communities were similar between 1% BPB and 2% BPB treated samples. [Conclusion] We changed the bacterial abundance and community structure in rhizosphere soil by applying different proportion of BPB, and the change in high proportion of BPB was more obvious.
引文
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[4]Zheng JF,Chen JH,Pan GX,Liu XY,Zhang XH,Li LQ,Bian RJ,Cheng K,Zheng JW.Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from southwest China.Science of the Total Environment,2016,571:206-217.
[5]Kelly CN,Calderón FC,Acosta-Martínez V,Mikha MM,Benjamin J,Rutherford DW,Rostad CE.Switchgrass biochar effects on plant biomass and microbial dynamics in two soils from different regions.Pedosphere,2015,25(3):329-342.
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[8]Lv WB.Effect of biochar on soil microbial biomass,respiration and community structure.Master Dissertation of Zhejiang University,2012.(in Chinese)吕伟波.生物炭对土壤微生物生态特征的影响.浙江大学硕士学位论文,2012.
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[11]Imparato V,Hansen V,Santos SS,Nielsen TK,Giagnoni L,Hauggaard-Nielsen H,Johansen A,Renella G,Winding A.Gasification biochar has limited effects on functional and structural diversity of soil microbial communities in a temperate agroecosystem.Soil Biology and Biochemistry,2016,99:128-136.
[12]Kolton M,Harel YM,Pasternak Z,Graber ER,Elad Y,Cytryn E.Impact of biochar application to soil on the root-associated bacterial community structure of fully developed greenhouse pepper plants.Applied and Environmental Microbiology,2011,77(14):4924-4930.
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[16]Avershina E,Frisli T,Rudi K.De novo semi-alignment of16S rRNA gene sequences for deep phylogenetic characterization of next generation sequencing data.Microbes and Environments,2013,28(2):211-216.
[17]Kim OS,Cho YJ,Lee K,Yoon SH,Kim M,Na H,Park SC,Jeon YS,Lee JH,Yi HN,Won S,Chun J.Introducing EzTaxon-e:a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species.International Journal of Systematic and Evolutionary Microbiology,2012,62:716-721.
[18]Schloss PD,Westcott SL,Ryabin T,Hall JR,Hartmann M,Hollister EB,Lesniewski RA,Oakley BB,Parks DH,Robinson CJ,Sahl JW,Stres B,Thallinger GG,van Horn DJ,Weber CF.Introducing mothur:open-source,platformindependent,community-supported software for describing and comparing microbial communities.Applied and Environmental Microbiology,2009,75(23):7537-7541.
[19]Mago?T,Salzberg SL.FLASH:fast length adjustment of short reads to improve genome assemblies.Bioinformatics,2011,27(21):2957-2963.
[20]Edgar RC.UPARSE:highly accurate OTU sequences from microbial amplicon reads.Nature Methods,2013,10(10):996-998.
[21]Edgar RC,Haas BJ,Clemente JC,Quince C,Knight R.UCHIME improves sensitivity and speed of chimera detection.Bioinformatics,2011,27(16):2194-2200.
[22]Anderson CR,Condron LM,Clough TJ,Fiers M,Stewart A,Hill RA,Sherlock RR.Biochar induced soil microbial community change:implications for biogeochemical cycling of carbon,nitrogen and phosphorus.Pedobiologia,2011,54(5-6):309-320.
[23]Dimitriu PA,Grayston SJ.Relationship between soil properties and patterns of bacterialβ-diversity across reclaimed and natural boreal forest soils.Microbial Ecology,2010,59(3):563-573.
[24]Sun DQ,Meng J,Xu EG,Chen WF.Microbial community structure and predicted bacterial metabolic functions in biochar pellets aged in soil after 34 months.Applied Soil Ecology,2016,100:135-143.
[25]Hu L,Cao LX,Zhang RD.Bacterial and fungal taxon changes in soil microbial community composition induced by short-term biochar amendment in red oxidized loam soil.World Journal of Microbiology and Biotechnology,2014,30(3):1085-1092.
[26]Kishimoto S,Sugiura G.Charcoal as a soil conditioner.International Achievements for the Future,1985,5:12-23.
[27]Brewer CE,Unger R,Schmidt-Rohr K,Brown RC.Criteria to select biochars for field studies based on biochar chemical properties.BioEnergy Research,2011,4(4):312-323.
[28]Watzinger A,Feichtmair S,Kitzler B,Zehetner F,Kloss S,Wimmer B,Zechmeister-Boltenstern S,Soja G.Soil microbial communities responded to biochar application in temperate soils and slowly metabolized 13C-labelled biochar as revealed by 13C PLFA analyses:results from a short-term incubation and pot experiment.European Journal of Soil Science,2014,65(1):40-51.
[29]Gomez JD,Denef K,Stewart CE,Zheng J,Cotrufo MF.Biochar addition rate influences soil microbial abundance and activity in temperate soils.European Journal of Soil Science,2014,65(1):28-39.
[30]Fierer N,Bradford MA,Jackson RB.Toward an ecological classification of soil bacteria.Ecology,2007,88(6):1354-1364.
[31]Prommer J,Wanek W,Hofhansl F,Trojan D,Offre P,Urich T,Schleper C,Sassmann S,Kitzler B,Soja G,Hood-Nowotny RC.Biochar decelerates soil organic nitrogen cycling but stimulates soil nitrification in a temperate arable field trial.PLoS One,2014,9(1):e86388.
[32]Liu XY,Zhang AF,Ji CY,Joseph S,Bian RJ,Li LQ,Pan GX,Paz-Ferreiro J.Biochar’s effect on crop productivity and the dependence on experimental conditions-a meta-analysis of literature data.Plant and Soil,2013,373(1-2):583-594.
[33]Arriagada C,Almonacid L,Cornejo P,Garcia-Romera I,Ocampo J.Influence of an organic amendment comprising saprophytic and mycorrhizal fungi on soil quality and growth of Eucalyptus globulus in the presence of sewage sludge contaminated with aluminium.Archives of Agronomy and Soil Science,2014,60(9):1229-1248.
[34]Ni T.Effects of different fertilizing treatments on the microbial flora in rice-wheat rotation field.Master Dissertation of Nanjing Agricultural University,2013.(in Chinese)倪添.稻麦轮作体系下不同施肥措施对土壤微生物区系的影响.南京农业大学硕士学位论文,2013.
[2]Lashari MS,Liu YM,Li LQ,Pan WN,Fu JY,Pan GX,Zheng JF,Zheng JW,Zhang XH,Yu XY.Effects of amendment of biochar-manure compost in conjunction with pyroligneous solution on soil quality and wheat yield of a salt-stressed cropland from Central China Great Plain.Field Crops Research,2013,144:113-118.
[3]Rondon MA,Lehmann J,Ramírez J,Hurtado M.Biological nitrogen fixation by common beans(Phaseolus vulgaris L.)increases with bio-char additions.Biology and Fertility of Soils,2007,43(6):699-708.
[4]Zheng JF,Chen JH,Pan GX,Liu XY,Zhang XH,Li LQ,Bian RJ,Cheng K,Zheng JW.Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from southwest China.Science of the Total Environment,2016,571:206-217.
[5]Kelly CN,Calderón FC,Acosta-Martínez V,Mikha MM,Benjamin J,Rutherford DW,Rostad CE.Switchgrass biochar effects on plant biomass and microbial dynamics in two soils from different regions.Pedosphere,2015,25(3):329-342.
[6]Ippolito JA,Stromberger ME,Lentz RD,Dungan RS.Hardwood biochar influences calcareous soil physicochemical and microbiological status.Journal of Environmental Quality,2014,43(2):681-689.
[7]Steinbeiss S,Gleixner G,Antonietti M.Effect of biochar amendment on soil carbon balance and soil microbial activity.Soil Biology and Biochemistry,2009,41(6):1301-1310.
[8]Lv WB.Effect of biochar on soil microbial biomass,respiration and community structure.Master Dissertation of Zhejiang University,2012.(in Chinese)吕伟波.生物炭对土壤微生物生态特征的影响.浙江大学硕士学位论文,2012.
[9]Muhammad N,Dai ZM,Xiao KC,Meng J,Brookes PC,Liu XM,Wang HZ,Wu JJ,Xu JM.Changes in microbial community structure due to biochars generated from different feedstocks and their relationships with soil chemical properties.Geoderma,2014,226-227:270-278.
[10]Anderson CR,Hamonts K,Clough TJ,Condron LM.Biochar does not affect soil N-transformations or microbial community structure under ruminant urine patches but does alter relative proportions of nitrogen cycling bacteria.Agriculture,Ecosystems&Environment,2014,191:63-72.
[11]Imparato V,Hansen V,Santos SS,Nielsen TK,Giagnoni L,Hauggaard-Nielsen H,Johansen A,Renella G,Winding A.Gasification biochar has limited effects on functional and structural diversity of soil microbial communities in a temperate agroecosystem.Soil Biology and Biochemistry,2016,99:128-136.
[12]Kolton M,Harel YM,Pasternak Z,Graber ER,Elad Y,Cytryn E.Impact of biochar application to soil on the root-associated bacterial community structure of fully developed greenhouse pepper plants.Applied and Environmental Microbiology,2011,77(14):4924-4930.
[13]鲍士旦.土壤农化分析.第3版.北京:中国农业出版社,2007.
[14]Novak JM,Bauer PJ,Hunt PG.Carbon dynamics under long-term conservation and disk tillage management in a Norfolk loamy sand.Soil Science Society of America Journal,2007,71(2):453-456.
[15]Caporaso JG,Kuczynski J,Stombaugh J,Bittinger K,Bushman FD,Costello EK,Fierer N,Pe?a AG,Goodrich JK,Gordon JI,Huttley GA,Kelley ST,Knights D,Koenig JE,Ley RE,Lozupone CA,McDonald D,Muegge BD,Pirrung M,Reeder J,Sevinsky JR,Turnbaugh PJ,Walters WA,Widmann J,Yatsunenko T,Zaneveld J,Knight R.QIIMEallows analysis of high-throughput community sequencing data.Nature Methods,2012,7(5):335-336.
[16]Avershina E,Frisli T,Rudi K.De novo semi-alignment of16S rRNA gene sequences for deep phylogenetic characterization of next generation sequencing data.Microbes and Environments,2013,28(2):211-216.
[17]Kim OS,Cho YJ,Lee K,Yoon SH,Kim M,Na H,Park SC,Jeon YS,Lee JH,Yi HN,Won S,Chun J.Introducing EzTaxon-e:a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species.International Journal of Systematic and Evolutionary Microbiology,2012,62:716-721.
[18]Schloss PD,Westcott SL,Ryabin T,Hall JR,Hartmann M,Hollister EB,Lesniewski RA,Oakley BB,Parks DH,Robinson CJ,Sahl JW,Stres B,Thallinger GG,van Horn DJ,Weber CF.Introducing mothur:open-source,platformindependent,community-supported software for describing and comparing microbial communities.Applied and Environmental Microbiology,2009,75(23):7537-7541.
[19]Mago?T,Salzberg SL.FLASH:fast length adjustment of short reads to improve genome assemblies.Bioinformatics,2011,27(21):2957-2963.
[20]Edgar RC.UPARSE:highly accurate OTU sequences from microbial amplicon reads.Nature Methods,2013,10(10):996-998.
[21]Edgar RC,Haas BJ,Clemente JC,Quince C,Knight R.UCHIME improves sensitivity and speed of chimera detection.Bioinformatics,2011,27(16):2194-2200.
[22]Anderson CR,Condron LM,Clough TJ,Fiers M,Stewart A,Hill RA,Sherlock RR.Biochar induced soil microbial community change:implications for biogeochemical cycling of carbon,nitrogen and phosphorus.Pedobiologia,2011,54(5-6):309-320.
[23]Dimitriu PA,Grayston SJ.Relationship between soil properties and patterns of bacterialβ-diversity across reclaimed and natural boreal forest soils.Microbial Ecology,2010,59(3):563-573.
[24]Sun DQ,Meng J,Xu EG,Chen WF.Microbial community structure and predicted bacterial metabolic functions in biochar pellets aged in soil after 34 months.Applied Soil Ecology,2016,100:135-143.
[25]Hu L,Cao LX,Zhang RD.Bacterial and fungal taxon changes in soil microbial community composition induced by short-term biochar amendment in red oxidized loam soil.World Journal of Microbiology and Biotechnology,2014,30(3):1085-1092.
[26]Kishimoto S,Sugiura G.Charcoal as a soil conditioner.International Achievements for the Future,1985,5:12-23.
[27]Brewer CE,Unger R,Schmidt-Rohr K,Brown RC.Criteria to select biochars for field studies based on biochar chemical properties.BioEnergy Research,2011,4(4):312-323.
[28]Watzinger A,Feichtmair S,Kitzler B,Zehetner F,Kloss S,Wimmer B,Zechmeister-Boltenstern S,Soja G.Soil microbial communities responded to biochar application in temperate soils and slowly metabolized 13C-labelled biochar as revealed by 13C PLFA analyses:results from a short-term incubation and pot experiment.European Journal of Soil Science,2014,65(1):40-51.
[29]Gomez JD,Denef K,Stewart CE,Zheng J,Cotrufo MF.Biochar addition rate influences soil microbial abundance and activity in temperate soils.European Journal of Soil Science,2014,65(1):28-39.
[30]Fierer N,Bradford MA,Jackson RB.Toward an ecological classification of soil bacteria.Ecology,2007,88(6):1354-1364.
[31]Prommer J,Wanek W,Hofhansl F,Trojan D,Offre P,Urich T,Schleper C,Sassmann S,Kitzler B,Soja G,Hood-Nowotny RC.Biochar decelerates soil organic nitrogen cycling but stimulates soil nitrification in a temperate arable field trial.PLoS One,2014,9(1):e86388.
[32]Liu XY,Zhang AF,Ji CY,Joseph S,Bian RJ,Li LQ,Pan GX,Paz-Ferreiro J.Biochar’s effect on crop productivity and the dependence on experimental conditions-a meta-analysis of literature data.Plant and Soil,2013,373(1-2):583-594.
[33]Arriagada C,Almonacid L,Cornejo P,Garcia-Romera I,Ocampo J.Influence of an organic amendment comprising saprophytic and mycorrhizal fungi on soil quality and growth of Eucalyptus globulus in the presence of sewage sludge contaminated with aluminium.Archives of Agronomy and Soil Science,2014,60(9):1229-1248.
[34]Ni T.Effects of different fertilizing treatments on the microbial flora in rice-wheat rotation field.Master Dissertation of Nanjing Agricultural University,2013.(in Chinese)倪添.稻麦轮作体系下不同施肥措施对土壤微生物区系的影响.南京农业大学硕士学位论文,2013.