叶面喷施NaH_2PO_4,K_2SO_4以及KH_2PO_4对葡萄磷钾吸收相关基因表达的影响
|
摘要
为了研究叶面喷施磷、钾以及磷钾复合肥的时效性。以‘魏可’葡萄为试材,选择葡萄花序展露期、果实膨大期和转色期,喷施磷钾相关肥料,统计分析其相关生理指标,测定磷、钾8个吸收相关基因的表达情况。叶面喷施三种肥料后,葡萄叶片的新梢生长率,叶绿素含量,果实大小等生理指标均有不同程度的提升。植株应答外源磷钾从基因水平、时间长短等与生长发育情况基本一致。喷施磷肥后基因应答效果在果实膨大期最佳,喷施钾肥则是在转色期最佳。磷钾吸收相关基因在6 h就已经开始表达,在12~24 h达到最高,并在需求高的时期可持续至48 h,磷素一般在24 h达到最高,钾素一般在12 h达到最高。综合生理指标和基因表达两个方面三种肥料的肥效为KH_2PO_4>K_2SO_4>Na H_2PO_4。可以利用磷钾吸收相关基因表达情况预先判断植株生长发育的情况,即可以利用该基因信息判断喷施磷钾肥对葡萄生长发育的影响。
In order to elucidate timeliness of foliar application of phosphorus(P),potassium(K),phosphorus and potassium compound fertilizer,'wink'(Muscat Bailey A×Rubel Muscat X)grapes were used as experimental materials,and P and K related fertilizers were sprayed on grapes at the flower-showing stage,fruit inflating stage and color-changed stage.Then,physiological indexes were analyzed and we detected the expression levels of 8 absorption genes of P and K.The new shoot of leaf increment rate,chlorophyll content,fruit size and other physiological traits of grapes were improved in different degrees after foliar spraying of three kinds of fertilizers.The plant response to exogenous P and K was basically consistent with the growth and development from gene level,time length and other aspects.The effect of gene response after the application of P fertilizer was the best in fruits inflating stage,and K fertilizer was best in color-changed period.The P and K uptake related genes expressed after 6hours and reached the highest expression level in 12~24 h.What's more,the expression could maintain at a high level for 48 h under the large requirement of the related genes.Phosphorus generally reached at the highest point in24 h,and potassium reached in 12 h.Comprehensive two aspects of physiology traits and the gene expression,we could conclude thatthe best fertilizer efficiency,followed byNaAll the results could help us predict the condition of plant growth and development by referring to the expression levels of the P and K uptake related genes,which could be used to determine the effect of P and K fertilizers on the growth and development of grape.
In order to elucidate timeliness of foliar application of phosphorus(P),potassium(K),phosphorus and potassium compound fertilizer,'wink'(Muscat Bailey A×Rubel Muscat X)grapes were used as experimental materials,and P and K related fertilizers were sprayed on grapes at the flower-showing stage,fruit inflating stage and color-changed stage.Then,physiological indexes were analyzed and we detected the expression levels of 8 absorption genes of P and K.The new shoot of leaf increment rate,chlorophyll content,fruit size and other physiological traits of grapes were improved in different degrees after foliar spraying of three kinds of fertilizers.The plant response to exogenous P and K was basically consistent with the growth and development from gene level,time length and other aspects.The effect of gene response after the application of P fertilizer was the best in fruits inflating stage,and K fertilizer was best in color-changed period.The P and K uptake related genes expressed after 6hours and reached the highest expression level in 12~24 h.What's more,the expression could maintain at a high level for 48 h under the large requirement of the related genes.Phosphorus generally reached at the highest point in24 h,and potassium reached in 12 h.Comprehensive two aspects of physiology traits and the gene expression,we could conclude thatthe best fertilizer efficiency,followed byNaAll the results could help us predict the condition of plant growth and development by referring to the expression levels of the P and K uptake related genes,which could be used to determine the effect of P and K fertilizers on the growth and development of grape.
引文
Boss P.K.,and Thomas M.R.,2002,Association of swarfdwarfism and floral induction with a grape‘green revolution’mulation,Nature,416(6883):847-850
Benito A.,Romero I.,Domínguez N.,García-Escudero E.,and Martín I.,2013,Leaf blade and petiole analysis for nutrient diagnosis in Vitis vinifera L.cv.Garnacha tinta,Australian Journal of Grape&Wine Research,19(2):285-298
Boss P.K.,and Thomas M.R.,2000,Tendrils,inflorescences and fruitfulness:A molecular perspective,Aust.J.Grape Wine R.,6(2):168-174
Chu J.Q.,Wang W.Y.,Fang J.G.,Zhang C.H.,Zhang Y.P.,and Song C.N.,2012,Effect of foliar applied urea on the expression of genes ralated to nitrogen metabolism in Fujiminori grapevine,Zhiwu Yingyang Yu Feiliao Xuebao(Plant Nutrition and Fertilizer Science),18(2):405-416(初建青,王文艳,房经贵,张春华,张彦平,宋长年,2012,叶面喷施尿素对葡萄氮代谢相关基因表达的影响,植物营养与肥料学报,18(2):405-416)
Du H.Y.,Xue S.C.,and Sun Z.F.,2007,Effects of different application rates of humic acid compound fertilizer on leaf nutrient accumulation and physiological mechanism of grape,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of E-co-Agriculture),15(1):49-51(杜会英,薛世川,孙忠富,2007,不同用量腐殖酸复合肥对葡萄叶片养分积累及其生理指标的影响,中国生态农业学报,15(1):49-51)
Fang J.G.,Liu H.,and Yang G.,2008,Wine grape star in the genomicsera-pinot noir,Zhongwai Putao Yu Putaojiu(SinoOverseas Grapevine&Wine),(4):57-59(房经贵,刘洪,杨光,2008,基因组学研究时代的葡萄酿造明星-黑比诺,中外葡萄与葡萄酒,(4):57-59)
Fang J.G.,2014,Applications of DNA technologies in agriculture,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),22(12):1463-1470(房经贵,2014,DNA技术在农业上的应用,农业生物技术学报,22(12):1463-1470)
Jaillon O.,Aury J.M.,Noel B.,Policriti A.,Clepet C.,Casagrande A.,Choisne N.,Aubourg S.,Vitulo N.,Jubin C.,Vezzi A.Legeai F.,Hugueney P.,Dasilva C.,Horner D.,Mica E.Jublot D.,Poulain J.,Bruyère C.,Billault A.,Segurens B.Gouyvenoux M.,Ugarte E.,Cattonaro F.,Anthouard V.,Vico V.,Del Fabbro C.,Alaux M.,Di Gaspero G.,Dumas V.Felice N.,Paillard S.,Juman I.,Moroldo M.,Scalabrin S.Canaguier A.,Le Clainche I.,Malacrida G.,Durand E.Pesole G.,Laucou V.,Chatelet P.,Merdinoglu D.,Delledonne M.,Pezzotti M.,Lecharny A.,Scarpelli C.,Artiguenave F.,PèM.E.,Valle G.,Morgante M.,Caboche M.Adam-Blondon A.F.,Weissenbach J.,Quétier F.,and Wincker P.,2007,The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla,Nature449(7161):463-467
Jain M.,Ghanashyam C.,and Bhattacharjee A.,2010,Comprhehensive expression analysis suggests overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses,BMC Genomics,11(1):1-17
Jiang W.F.,and Zhou J.B.,2009,Effects of application of nitrogen,phosphorus and potassium on the yield and quality of thompson seedless,Zhongwai Putao Yu Putaojiu(Sino-O-verseas Grapevine&Wine),(3):4-8(蒋万峰,周建斌,2009氮磷钾化肥配合施用对无核白葡萄品质与产量的影响中外葡萄与葡萄酒,(3):4-8)
Khan J.,Wei J.S.,Ringner M.,Saal L.H.,Ladanyi M.,Westermann F.,Berthold F.,Schwab S.,Antonescu C.R.,Teterson C.and Meltzer P.S.,2001,Classification and diagnostic prediction of cancers using gene expression profiling and artificial neural networks,Nat.Med.,7(6):673-679
Kou C.L.,and Zhang F.S.,1999,Study on the variety of whea and peanut in utilization to different phosphorus compound Turang Tongbao(Chinese Journal of Soil Science),30(4):181-184(寇长林,张福锁,1999,小麦和花生利用磷形态差异的研究,土壤通报,30(4):181-184)
Li J.,An F.,Lin W.F.,Zhou L.J.,Chen J.M.,Pan J.,Huang J.X.and Zheng D.H.,2016.Effects of different dosages of NPKfertilizer on chloroplastⅡcontent of Wuzhimaotao,Henongxue Bao(Journal of Nuclear Agricultural Science),30(1):193-200(李娟,安锋,林位夫,周立军,陈俊明,潘剑,黄坚雄,郑定华,2016,不同氮磷钾肥用量对五指毛桃叶绿素含量的影响,核农学报,30(1):193-200)
Li J.2011,Soil testing and fertilization techniques of corn,Xiandai Nongye(Modern Agriculture),(2):14(李杰,2010,玉米测土配方施肥技术,现代农业,(2):14)
Li Y.T.,Xiao Y.,Li X.Y.,and Zhao C.Q.,2009,The application of foliar fertilization technology in fruit trees,Zhongguo Nongye Xinxi(China Agriculture Information),(2):28-30(李燕婷,肖艳,李秀英,赵秉强,2009,叶面施肥技术在果树上的应用,中国农业信息,(2):28-30)
Loppes R.,Radoux M.,Ohresser M.C.P.,and Matagne R.F.,1999,Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii:effects of various environmental factors on the expression of a reporter gene under the control of the Nia promoter,Plant Mol.Biol.,41(5):701-711
Ma W.J.,Tong Y.A.,Gao Y.M.,and Kong Y.,2009,Studies on phosphorus absorption and accumulation in main growing period of grape tree,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University),(9):89-94(马文娟,同延安,高义民,孔莹,2009,葡萄树主要生长期内磷素吸收及累积规律,西北农林科技大学学报,(9):89-94)
Ma W.J.,Tong Y.A.,Wang B.X.,and Yang L.F.,2013,Annual change of potassium content and accumulation in grape tree,Xibei Nonglin Keji Daxue Xuebao(Journal Nortweweat A&F University),41(9):127-132(马文娟,同延安,王百祥,杨莉芳,2013,葡萄树主要生长期内钾素的吸收与累积规律,西北农林科技大学学报,41(9):127-132)
Ma Z.Q.,Jia M.F.,Wang J.H.,and Zhai H.,2014.Effects of the timing of phosphatic fertilizer dressing on phosphorus utilization in‘Moldova’grapes,Guoshu Xuebao(Journal of Fruit Science),31(5):848-853(马振强,贾明方,王金欢,翟衡,2014,磷肥追施时期对‘摩尔多瓦’葡萄磷素吸收利用的影响,果树学报,31(5):848-853)
Niu Z.M.,Xu X.F.,Wang Y.,Li T.Z.,Kong J.,and Han Z.H.,2008,Effects of leaf-applied potassium,gibberellin and source-sink ratio on potassium absorption and distribution in grape fruits,Sci.Hortic.,115(2):164-167
Pratelli R.,Lacombe B.,Torregtose L.,Gaymard F.,Romieu C.,Thibaud J.B.,and Sentenac H.,2002,A grapevine gene encoding a guard cell K(+)channel displays developmental regulation in the grapevine berry,Plant Physiol.,128(2):564-577
Song Y.,Cui S.M.,Du J.W.,and Jin P.,2008,The grape leaves growth and the anatomical changes both of the grape leaves and roots through different levels of nitrogen fertilizer applications,Huabei Nongxuebao(Acta Agriculturae Boreai-Sinica),23(3):204-208(宋阳,崔世茂,杜金伟,金丽萍,2008,氮肥不同施用量对葡萄叶片生长及根、叶细胞结构的影响,华北农学报,23(3):204-208)
Tibshirani R.,Hastie T.,Narasimhan B.,and Chu G.,2002,Diagnosis of multiple cancer types by shrunken centroids of gene expression,Proc.Natl.Acad.Sci.USA,99(10):6567-6572
Wample R.L.,Spayd S.E.,Evans R.G.,and Stevens R.G.,1993,Nitrogen fertilization of white riesling grapes in washington:nitrogen seasonal effects on bud cold hardiness and carbohydrate reserve,Am.J.Enol.Viticult.,44(2):159-167
Wang C.,Han J.,Shangguan L.F.,Yang G.,Kayesh E.,Zhang YY.,Leng X.P.,and Fang J.G.,2014,Depiction of grapevine phenology by genes expression information and test of its workability in guiding fertilization,Plant Mol.Biol.Rep.,32(5):1070-1084
Wang J.,Li B.R.,Li X.P.,Zhu X.D.,Zhu C.G.,and Jia H.F.2016,Evaluation of N fertilizers effects based on the expression of N metabolic genes,Yuanyi Xuebao(Acta Horticultural Sinica),43(1):1-14(王剑,李炳锐,李晓鹏,朱旭东,朱传根,贾海锋,2016,利用葡萄氮代谢基因的表达评价不同氮肥肥效,园艺学报,43(1):1-14)
Wright G.,Tan B.,Rosenwald A.,Hurt E.H.,Wiestner A.,and S-taudt L.M.,2003,A gene expression-based method to diagnose clinically distinct subgroups of diffuse large B cell lymphoma,Proc.Natl.Acad.Sci.USA,100(17):9991-9996
Xie H.X.,Chen B.,Wen Q.K.,and Liao K.,2005,Effect of N,PK fertilizer on yield and quality of red globe grape,Beifang Yuanyi(Northern Horticulture),(4):73-74(谢海霞,陈冰文启凯,廖康,2005,氮、磷、钾肥对“全球红葡萄”产量与品质的影响,北方园艺,(4):73-74)
Ye J.N.,1985,Arnon formula in the measurement of chlorophyll content,Zhiwu Shenglixue Tongxun(Plant Physionlogy Communications),(6):69(叶济宁,1985,关于叶绿素含量测定中的Arnon计算公式,植物生理学通讯,(6):69)
Zhang C.X.,Yang T.Y.,Luo J.,Wang X.L.,Jiang A.L.,and Ye Z.W.,2010,Effects of different fertilizers and application methods on photosynthetic characteristics and quality of Kyoho grape,Xinan Nongye Xuebao(Southwest China Journa of Agricultural Sciences),23(2):440-443(张朝轩,杨天仪骆军,王相林,蒋爱丽,叶正文,2010,不同肥料及施用方式对巨峰葡萄叶片光合特性和果实品质的影响,西南农业学报,23(2):440-443)
Zhang C.,Jia H.F.,Wang J.,Jiu S.T.,and Wang M.Q.,2016,Effectiveness and concentration of foliar-application of potassium fertilizers on grapevine evaluated by expression of potassium uptake related genes,Zhiwu Yingyang Yu Feiliao Xuebao(Plant Nutrition and Fertilizer Science),22(4):1091-1101(张成,贾海峰,王剑,纠松涛,王梦琦,2016,利用钾吸收基因表达评价葡萄叶面喷施钾肥效果和喷施浓度,植物营养与肥料学报,22(4):1091-1101)
Zhang C.,Jia H.F.,Zeng J.J.,Perraiz T.,Xie Z.Q.,Zhu X.D.,and Wang C.,2016,Fertilization of grapevine based on gene expression,Plant Genome,9(3):1-13
Zhang H.X.,Chi D.C.,Wang Q.,Fang J.,and Fang X.Y.,2011,Yield and quality response of cucumber to irrigation and nitrogen fertilization under subsurface drip irrigation in solar greenhouse,Agricultural Sciences in China,10(6):921-930
Zhang N.,2011,Response of the two arabidopsis purple acid phosphatase,At PAP2 and At PAP9,to phosphorus stress,Thesis for M.S.,Inner Mongolia Agricultural University,Supervisor:Wang R.G.,pp.5-10(张娜,2011,拟南芥紫色酸性磷酸酶At PAP2和At PAP9对磷胁迫的响应,硕士学位论文,内蒙古农业大学,导师:王瑞刚,pp.5-10)
Zhong S.,Guo C.H.,Lu C.Y.,Guo G.G.,Zhang C.H.,and Wang Y.J.,2014,Effect of different fertilizer on yield and quality of red globe grape,Xibei Nongye Xuebao(Acta Agriculturae Boreali-Occidentalis Sinica),23(11):104-109(钟帅,郭春会,陆春燕,郭改改,张朝红,王跃进,2014,不同施肥方式对红地球葡萄产量和品质的影响,西北农业学报,23(11):104-109)
Benito A.,Romero I.,Domínguez N.,García-Escudero E.,and Martín I.,2013,Leaf blade and petiole analysis for nutrient diagnosis in Vitis vinifera L.cv.Garnacha tinta,Australian Journal of Grape&Wine Research,19(2):285-298
Boss P.K.,and Thomas M.R.,2000,Tendrils,inflorescences and fruitfulness:A molecular perspective,Aust.J.Grape Wine R.,6(2):168-174
Chu J.Q.,Wang W.Y.,Fang J.G.,Zhang C.H.,Zhang Y.P.,and Song C.N.,2012,Effect of foliar applied urea on the expression of genes ralated to nitrogen metabolism in Fujiminori grapevine,Zhiwu Yingyang Yu Feiliao Xuebao(Plant Nutrition and Fertilizer Science),18(2):405-416(初建青,王文艳,房经贵,张春华,张彦平,宋长年,2012,叶面喷施尿素对葡萄氮代谢相关基因表达的影响,植物营养与肥料学报,18(2):405-416)
Du H.Y.,Xue S.C.,and Sun Z.F.,2007,Effects of different application rates of humic acid compound fertilizer on leaf nutrient accumulation and physiological mechanism of grape,Zhongguo Shengtai Nongye Xuebao(Chinese Journal of E-co-Agriculture),15(1):49-51(杜会英,薛世川,孙忠富,2007,不同用量腐殖酸复合肥对葡萄叶片养分积累及其生理指标的影响,中国生态农业学报,15(1):49-51)
Fang J.G.,Liu H.,and Yang G.,2008,Wine grape star in the genomicsera-pinot noir,Zhongwai Putao Yu Putaojiu(SinoOverseas Grapevine&Wine),(4):57-59(房经贵,刘洪,杨光,2008,基因组学研究时代的葡萄酿造明星-黑比诺,中外葡萄与葡萄酒,(4):57-59)
Fang J.G.,2014,Applications of DNA technologies in agriculture,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),22(12):1463-1470(房经贵,2014,DNA技术在农业上的应用,农业生物技术学报,22(12):1463-1470)
Jaillon O.,Aury J.M.,Noel B.,Policriti A.,Clepet C.,Casagrande A.,Choisne N.,Aubourg S.,Vitulo N.,Jubin C.,Vezzi A.Legeai F.,Hugueney P.,Dasilva C.,Horner D.,Mica E.Jublot D.,Poulain J.,Bruyère C.,Billault A.,Segurens B.Gouyvenoux M.,Ugarte E.,Cattonaro F.,Anthouard V.,Vico V.,Del Fabbro C.,Alaux M.,Di Gaspero G.,Dumas V.Felice N.,Paillard S.,Juman I.,Moroldo M.,Scalabrin S.Canaguier A.,Le Clainche I.,Malacrida G.,Durand E.Pesole G.,Laucou V.,Chatelet P.,Merdinoglu D.,Delledonne M.,Pezzotti M.,Lecharny A.,Scarpelli C.,Artiguenave F.,PèM.E.,Valle G.,Morgante M.,Caboche M.Adam-Blondon A.F.,Weissenbach J.,Quétier F.,and Wincker P.,2007,The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla,Nature449(7161):463-467
Jain M.,Ghanashyam C.,and Bhattacharjee A.,2010,Comprhehensive expression analysis suggests overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses,BMC Genomics,11(1):1-17
Jiang W.F.,and Zhou J.B.,2009,Effects of application of nitrogen,phosphorus and potassium on the yield and quality of thompson seedless,Zhongwai Putao Yu Putaojiu(Sino-O-verseas Grapevine&Wine),(3):4-8(蒋万峰,周建斌,2009氮磷钾化肥配合施用对无核白葡萄品质与产量的影响中外葡萄与葡萄酒,(3):4-8)
Khan J.,Wei J.S.,Ringner M.,Saal L.H.,Ladanyi M.,Westermann F.,Berthold F.,Schwab S.,Antonescu C.R.,Teterson C.and Meltzer P.S.,2001,Classification and diagnostic prediction of cancers using gene expression profiling and artificial neural networks,Nat.Med.,7(6):673-679
Kou C.L.,and Zhang F.S.,1999,Study on the variety of whea and peanut in utilization to different phosphorus compound Turang Tongbao(Chinese Journal of Soil Science),30(4):181-184(寇长林,张福锁,1999,小麦和花生利用磷形态差异的研究,土壤通报,30(4):181-184)
Li J.,An F.,Lin W.F.,Zhou L.J.,Chen J.M.,Pan J.,Huang J.X.and Zheng D.H.,2016.Effects of different dosages of NPKfertilizer on chloroplastⅡcontent of Wuzhimaotao,Henongxue Bao(Journal of Nuclear Agricultural Science),30(1):193-200(李娟,安锋,林位夫,周立军,陈俊明,潘剑,黄坚雄,郑定华,2016,不同氮磷钾肥用量对五指毛桃叶绿素含量的影响,核农学报,30(1):193-200)
Li J.2011,Soil testing and fertilization techniques of corn,Xiandai Nongye(Modern Agriculture),(2):14(李杰,2010,玉米测土配方施肥技术,现代农业,(2):14)
Li Y.T.,Xiao Y.,Li X.Y.,and Zhao C.Q.,2009,The application of foliar fertilization technology in fruit trees,Zhongguo Nongye Xinxi(China Agriculture Information),(2):28-30(李燕婷,肖艳,李秀英,赵秉强,2009,叶面施肥技术在果树上的应用,中国农业信息,(2):28-30)
Loppes R.,Radoux M.,Ohresser M.C.P.,and Matagne R.F.,1999,Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii:effects of various environmental factors on the expression of a reporter gene under the control of the Nia promoter,Plant Mol.Biol.,41(5):701-711
Ma W.J.,Tong Y.A.,Gao Y.M.,and Kong Y.,2009,Studies on phosphorus absorption and accumulation in main growing period of grape tree,Xibei Nonglin Keji Daxue Xuebao(Journal of Northwest A&F University),(9):89-94(马文娟,同延安,高义民,孔莹,2009,葡萄树主要生长期内磷素吸收及累积规律,西北农林科技大学学报,(9):89-94)
Ma W.J.,Tong Y.A.,Wang B.X.,and Yang L.F.,2013,Annual change of potassium content and accumulation in grape tree,Xibei Nonglin Keji Daxue Xuebao(Journal Nortweweat A&F University),41(9):127-132(马文娟,同延安,王百祥,杨莉芳,2013,葡萄树主要生长期内钾素的吸收与累积规律,西北农林科技大学学报,41(9):127-132)
Ma Z.Q.,Jia M.F.,Wang J.H.,and Zhai H.,2014.Effects of the timing of phosphatic fertilizer dressing on phosphorus utilization in‘Moldova’grapes,Guoshu Xuebao(Journal of Fruit Science),31(5):848-853(马振强,贾明方,王金欢,翟衡,2014,磷肥追施时期对‘摩尔多瓦’葡萄磷素吸收利用的影响,果树学报,31(5):848-853)
Niu Z.M.,Xu X.F.,Wang Y.,Li T.Z.,Kong J.,and Han Z.H.,2008,Effects of leaf-applied potassium,gibberellin and source-sink ratio on potassium absorption and distribution in grape fruits,Sci.Hortic.,115(2):164-167
Pratelli R.,Lacombe B.,Torregtose L.,Gaymard F.,Romieu C.,Thibaud J.B.,and Sentenac H.,2002,A grapevine gene encoding a guard cell K(+)channel displays developmental regulation in the grapevine berry,Plant Physiol.,128(2):564-577
Song Y.,Cui S.M.,Du J.W.,and Jin P.,2008,The grape leaves growth and the anatomical changes both of the grape leaves and roots through different levels of nitrogen fertilizer applications,Huabei Nongxuebao(Acta Agriculturae Boreai-Sinica),23(3):204-208(宋阳,崔世茂,杜金伟,金丽萍,2008,氮肥不同施用量对葡萄叶片生长及根、叶细胞结构的影响,华北农学报,23(3):204-208)
Tibshirani R.,Hastie T.,Narasimhan B.,and Chu G.,2002,Diagnosis of multiple cancer types by shrunken centroids of gene expression,Proc.Natl.Acad.Sci.USA,99(10):6567-6572
Wample R.L.,Spayd S.E.,Evans R.G.,and Stevens R.G.,1993,Nitrogen fertilization of white riesling grapes in washington:nitrogen seasonal effects on bud cold hardiness and carbohydrate reserve,Am.J.Enol.Viticult.,44(2):159-167
Wang C.,Han J.,Shangguan L.F.,Yang G.,Kayesh E.,Zhang YY.,Leng X.P.,and Fang J.G.,2014,Depiction of grapevine phenology by genes expression information and test of its workability in guiding fertilization,Plant Mol.Biol.Rep.,32(5):1070-1084
Wang J.,Li B.R.,Li X.P.,Zhu X.D.,Zhu C.G.,and Jia H.F.2016,Evaluation of N fertilizers effects based on the expression of N metabolic genes,Yuanyi Xuebao(Acta Horticultural Sinica),43(1):1-14(王剑,李炳锐,李晓鹏,朱旭东,朱传根,贾海锋,2016,利用葡萄氮代谢基因的表达评价不同氮肥肥效,园艺学报,43(1):1-14)
Wright G.,Tan B.,Rosenwald A.,Hurt E.H.,Wiestner A.,and S-taudt L.M.,2003,A gene expression-based method to diagnose clinically distinct subgroups of diffuse large B cell lymphoma,Proc.Natl.Acad.Sci.USA,100(17):9991-9996
Xie H.X.,Chen B.,Wen Q.K.,and Liao K.,2005,Effect of N,PK fertilizer on yield and quality of red globe grape,Beifang Yuanyi(Northern Horticulture),(4):73-74(谢海霞,陈冰文启凯,廖康,2005,氮、磷、钾肥对“全球红葡萄”产量与品质的影响,北方园艺,(4):73-74)
Ye J.N.,1985,Arnon formula in the measurement of chlorophyll content,Zhiwu Shenglixue Tongxun(Plant Physionlogy Communications),(6):69(叶济宁,1985,关于叶绿素含量测定中的Arnon计算公式,植物生理学通讯,(6):69)
Zhang C.X.,Yang T.Y.,Luo J.,Wang X.L.,Jiang A.L.,and Ye Z.W.,2010,Effects of different fertilizers and application methods on photosynthetic characteristics and quality of Kyoho grape,Xinan Nongye Xuebao(Southwest China Journa of Agricultural Sciences),23(2):440-443(张朝轩,杨天仪骆军,王相林,蒋爱丽,叶正文,2010,不同肥料及施用方式对巨峰葡萄叶片光合特性和果实品质的影响,西南农业学报,23(2):440-443)
Zhang C.,Jia H.F.,Wang J.,Jiu S.T.,and Wang M.Q.,2016,Effectiveness and concentration of foliar-application of potassium fertilizers on grapevine evaluated by expression of potassium uptake related genes,Zhiwu Yingyang Yu Feiliao Xuebao(Plant Nutrition and Fertilizer Science),22(4):1091-1101(张成,贾海峰,王剑,纠松涛,王梦琦,2016,利用钾吸收基因表达评价葡萄叶面喷施钾肥效果和喷施浓度,植物营养与肥料学报,22(4):1091-1101)
Zhang C.,Jia H.F.,Zeng J.J.,Perraiz T.,Xie Z.Q.,Zhu X.D.,and Wang C.,2016,Fertilization of grapevine based on gene expression,Plant Genome,9(3):1-13
Zhang H.X.,Chi D.C.,Wang Q.,Fang J.,and Fang X.Y.,2011,Yield and quality response of cucumber to irrigation and nitrogen fertilization under subsurface drip irrigation in solar greenhouse,Agricultural Sciences in China,10(6):921-930
Zhang N.,2011,Response of the two arabidopsis purple acid phosphatase,At PAP2 and At PAP9,to phosphorus stress,Thesis for M.S.,Inner Mongolia Agricultural University,Supervisor:Wang R.G.,pp.5-10(张娜,2011,拟南芥紫色酸性磷酸酶At PAP2和At PAP9对磷胁迫的响应,硕士学位论文,内蒙古农业大学,导师:王瑞刚,pp.5-10)
Zhong S.,Guo C.H.,Lu C.Y.,Guo G.G.,Zhang C.H.,and Wang Y.J.,2014,Effect of different fertilizer on yield and quality of red globe grape,Xibei Nongye Xuebao(Acta Agriculturae Boreali-Occidentalis Sinica),23(11):104-109(钟帅,郭春会,陆春燕,郭改改,张朝红,王跃进,2014,不同施肥方式对红地球葡萄产量和品质的影响,西北农业学报,23(11):104-109)